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The map label for this gene is oprF [H]

Identifier: 120613225

GI number: 120613225

Start: 5111057

End: 5111947

Strand: Direct

Name: oprF [H]

Synonym: Aave_4592

Alternate gene names: 120613225

Gene position: 5111057-5111947 (Clockwise)

Preceding gene: 120613224

Following gene: 120613226

Centisome position: 95.48

GC content: 70.82

Gene sequence:

>891_bases
ATGCGCCATACCCTTCGCCGCTCCACCGCCCTCGCCGCCGCGCTCGTTGCCTCAGGCCTCCTGGCCGCCTGCGCGACGCG
CGCGCCACTCGATTCGGTCGAAACCGCCCGCATCGCCGTCAACCGTGCGGCCAATGACCCGGCCGTCGTGCAGAACGCAC
CGCTGGAACTGAAGGCCGCCACCGATACGCTCGCCCGTGCGGACCGTGTCTGGAGCGACAAGAACGACGCCGCCGAGGCC
AACCACCTCGCCTATCTCGCCACGCAGCGCGCCGATATCGCCTCCAACGTGGCCCGCTCGCGGGTGCTGGACGCCGACAT
CAAGAAGGCGGGCGCCGATGCCGAGCGCCTGCGCCTGACCAGCGAAGCCGACCGGGCCCGCATGCAGGCCGAGGCCAACG
CCCGCAGCGCCCAGCAGGCACAACTGCAGGCCGCGAGCGCCGAACAGCGTGCCGCCGACAATGCCGCCCGCGTCCGTGCG
CTGGAAGCGCAACTGCGCGACATCGAGGCGCAGCAGACCGAGCGCGGCCTGCTGGTAACGCTGGGCGACGTGCTGTTCGC
CTTCAACAAGGCCGAACTGTCCGCCCAGGCCGGGCCGCGGCTGGACAAGCTGGCGAACTTCCTGCGCCAGTTCCCGGACC
GCAAGCTCATCATCGAAGGCCATACCGACAGCGTGGGCGGCGACGCCTACAACCAGAGCCTGTCGGAGCGCCGTGCGCAG
GCCGTGCAGAGCGCGCTGGTGCAGCGCGGCGTCGCACCGGACCGCATCACCGCCCGCGGATACGGCAAGACCTACCCGGT
GGCCGACAACAGTTCGCCCGAAGGCCGCGCCATGAACCGGCGCGTGGAAATCGTCATCGCCGACGAGAAGGGCAATCTGC
GGGGCCGCTGA

Upstream 100 bases:

>100_bases
CACCCCGACCGCCGTGCCCGGCATGCCGGTGCCCCCCAACCCGGTCCCCGCCCGCTGAGCCCGACCGAACGAACGACCAC
CCGACAGGGAGAACCACACC

Downstream 100 bases:

>100_bases
TCCCCAGGCCCAGGCCCTGACCGCGCCCGCGCCGGCAGGGCCTTTTTGCCGCGCGGCGCGGCTCTGTCCGATACTGCATC
CCATGACCGCCCAAGACGTC

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRHTLRRSTALAAALVASGLLAACATRAPLDSVETARIAVNRAANDPAVVQNAPLELKAATDTLARADRVWSDKNDAAEA
NHLAYLATQRADIASNVARSRVLDADIKKAGADAERLRLTSEADRARMQAEANARSAQQAQLQAASAEQRAADNAARVRA
LEAQLRDIEAQQTERGLLVTLGDVLFAFNKAELSAQAGPRLDKLANFLRQFPDRKLIIEGHTDSVGGDAYNQSLSERRAQ
AVQSALVQRGVAPDRITARGYGKTYPVADNSSPEGRAMNRRVEIVIADEKGNLRGR

Sequences:

>Translated_296_residues
MRHTLRRSTALAAALVASGLLAACATRAPLDSVETARIAVNRAANDPAVVQNAPLELKAATDTLARADRVWSDKNDAAEA
NHLAYLATQRADIASNVARSRVLDADIKKAGADAERLRLTSEADRARMQAEANARSAQQAQLQAASAEQRAADNAARVRA
LEAQLRDIEAQQTERGLLVTLGDVLFAFNKAELSAQAGPRLDKLANFLRQFPDRKLIIEGHTDSVGGDAYNQSLSERRAQ
AVQSALVQRGVAPDRITARGYGKTYPVADNSSPEGRAMNRRVEIVIADEKGNLRGR
>Mature_296_residues
MRHTLRRSTALAAALVASGLLAACATRAPLDSVETARIAVNRAANDPAVVQNAPLELKAATDTLARADRVWSDKNDAAEA
NHLAYLATQRADIASNVARSRVLDADIKKAGADAERLRLTSEADRARMQAEANARSAQQAQLQAASAEQRAADNAARVRA
LEAQLRDIEAQQTERGLLVTLGDVLFAFNKAELSAQAGPRLDKLANFLRQFPDRKLIIEGHTDSVGGDAYNQSLSERRAQ
AVQSALVQRGVAPDRITARGYGKTYPVADNSSPEGRAMNRRVEIVIADEKGNLRGR

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=113, Percent_Identity=42.4778761061947, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI48994946, Length=133, Percent_Identity=35.3383458646617, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786962, Length=102, Percent_Identity=39.2156862745098, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788957, Length=104, Percent_Identity=38.4615384615385, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 31851; Mature: 31851

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHTLRRSTALAAALVASGLLAACATRAPLDSVETARIAVNRAANDPAVVQNAPLELKAA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCCCEEEHH
TDTLARADRVWSDKNDAAEANHLAYLATQRADIASNVARSRVLDADIKKAGADAERLRLT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SEADRARMQAEANARSAQQAQLQAASAEQRAADNAARVRALEAQLRDIEAQQTERGLLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LGDVLFAFNKAELSAQAGPRLDKLANFLRQFPDRKLIIEGHTDSVGGDAYNQSLSERRAQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
AVQSALVQRGVAPDRITARGYGKTYPVADNSSPEGRAMNRRVEIVIADEKGNLRGR
HHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MRHTLRRSTALAAALVASGLLAACATRAPLDSVETARIAVNRAANDPAVVQNAPLELKAA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCCCEEEHH
TDTLARADRVWSDKNDAAEANHLAYLATQRADIASNVARSRVLDADIKKAGADAERLRLT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SEADRARMQAEANARSAQQAQLQAASAEQRAADNAARVRALEAQLRDIEAQQTERGLLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
LGDVLFAFNKAELSAQAGPRLDKLANFLRQFPDRKLIIEGHTDSVGGDAYNQSLSERRAQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
AVQSALVQRGVAPDRITARGYGKTYPVADNSSPEGRAMNRRVEIVIADEKGNLRGR
HHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]