Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 120611780

GI number: 120611780

Start: 3442615

End: 3444267

Strand: Reverse

Name: nadE [H]

Synonym: Aave_3120

Alternate gene names: 120611780

Gene position: 3444267-3442615 (Counterclockwise)

Preceding gene: 120611782

Following gene: 120611779

Centisome position: 64.35

GC content: 69.33

Gene sequence:

>1653_bases
ATGCCATTCGTCATCAGCATCGCGCAATTCAACTTCATCGTCGGCGATGTCGAAGGCAATGCCCGAAAGATCATCTCCGC
CGCCCATGAGGCCCACGCCCAGGGTGCCGCGCTGCTGCTCACTCCCGAACTGGCCCTGTGCGGCTATGCCGCCGAGGATC
TCTTCCTGCGCCCCGCGTTCCTCGATGCCTGCGACCGGGCGCTGCAGGACATCGCGCAGGCCACGGCGGCATGCGGCGAC
CTGGCGGTGGTGGTCGGCCACCCGTGGCGCAGCGTGGCCGGTGGCGCCGAGCGGTGCCACAACGCCGCCAGCGTGCTGCG
TGGCGGGCGCGTCGAGCAGACCTACGCCAAGCAGGAACTGCCCAACTACGCCGTGTTCGACGAGCAGCGGTATTTCGACC
CGGGCACGCAACCCTGCGTGTTCGACGTGCAGGGCGTGCGCGTCGGGCTGCTGATCTGCGAGGACGCCTGGTTTCCCGGG
CCGGCGGCCCGCGCGGCGCAGGCGGGCGCCCAGTTGCTCGCGACGCTGAACGCCTCGCCCTTCCACCTGGGCAAGAGCGC
CGAGCGCGAGCAGATCATGCGCGAACGTGTTGCGGAAACGGGGCTGCCCCTGGTCTATGCCCACCTGGTGGGCGGGCAGG
ACGAGGTGGTCTTCGAAGGCCGCTCGTTCGCGCTGGATGCCACCGGCTTCGTCGGCGTCCGCGCGCCGGGCTTCGCCGAA
GCGCTGGTGCGGGTCGTGGCGCGTGCCGAAGACGGCGTGCTCCGGTTCGAGCCCGACGTGGCGCCCATTCCCGCGCCCGA
GGCCGATCTCTGGAGCGCACTGGTGCTGGGCGTGCGCGACTACGTGGGCAAGAACGGATTCCCGGGCGTGCTGCTGGGCC
TGTCCGGCGGCATCGATTCGGCGCTGGTGCTCGCCATCGCGGTGGACGCGCTCGGCCCCGACAAGGTGCGCGCGGTGATG
ATGCCTTCGCCCTATACGGCCGACATCAGCTGGATCGATGCGCGCGACATGGCCGAGCGGCTCGGCGTGCGCTATGACGA
GATCGCCATCGCCCCGCAGTTCGAGGCCTTCAAGGCCGCGCTCGCATCCGAGTTCGCCGGGCGTGCGGAAGACGCCACCG
AGGAAAACCTGCAGGCGCGCATCCGCGGCACGCTGCTCATGGCGCTGTCCAACAAGTTCGGCTCGGTCGTGCTCACCACC
GGCAACAAGAGCGAAATGGCCACGGGCTATTGCACGCTCTACGGCGACATGGCCGGCGGCTTCGCGGTCATCAAGGACGT
GGCCAAGACGACCGTCTATGGACTGGCGCGGTGGCGCAATGCGCACGACCCCTTCGGCACCGGCGCCTCGCCCATTCCCG
AACGCATCATCACCCGCCCGCCGAGCGCGGAGCTGCGGCCGGACCAGAAGGACCAGGACAGCCTGCCGCCCTACGACGTG
CTGGACGCCATCGTCGGGCGCTACATGGAAAACGACGAACCCATCGAGTCCATCATCGCCGCGGGTTACGCGCGCGCGGA
CGTCGAGCGCGTCACCCGTCTCATCCAGGTCAACGAGTACAAGCGCCGGCAATCCCCCGTCGGCATCCGCGTCACCCGCC
GCAGCTTCGGCAAGGACTGGCGCTACCCGATCACCAACCGTTTCCGGGCCTGA

Upstream 100 bases:

>100_bases
TATCCACAGAGTTGTCCAGCGTACGCGGGGATAACGTGACGCGCCGTGCGTGGCGGACACGGATGACATAATCTCCCGAG
TCCTTTCCCGCAGCCTTTCC

Downstream 100 bases:

>100_bases
CGGCCCGTGAGCATCCGTCCGCGGCGCAGCGGCGGCCCCCAGGGCCCGCAGCGTCCGCCGGCATCACGTTTCGAGGAGTT
CCCGCCATGAAAATGATTAC

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MPFVISIAQFNFIVGDVEGNARKIISAAHEAHAQGAALLLTPELALCGYAAEDLFLRPAFLDACDRALQDIAQATAACGD
LAVVVGHPWRSVAGGAERCHNAASVLRGGRVEQTYAKQELPNYAVFDEQRYFDPGTQPCVFDVQGVRVGLLICEDAWFPG
PAARAAQAGAQLLATLNASPFHLGKSAEREQIMRERVAETGLPLVYAHLVGGQDEVVFEGRSFALDATGFVGVRAPGFAE
ALVRVVARAEDGVLRFEPDVAPIPAPEADLWSALVLGVRDYVGKNGFPGVLLGLSGGIDSALVLAIAVDALGPDKVRAVM
MPSPYTADISWIDARDMAERLGVRYDEIAIAPQFEAFKAALASEFAGRAEDATEENLQARIRGTLLMALSNKFGSVVLTT
GNKSEMATGYCTLYGDMAGGFAVIKDVAKTTVYGLARWRNAHDPFGTGASPIPERIITRPPSAELRPDQKDQDSLPPYDV
LDAIVGRYMENDEPIESIIAAGYARADVERVTRLIQVNEYKRRQSPVGIRVTRRSFGKDWRYPITNRFRA

Sequences:

>Translated_550_residues
MPFVISIAQFNFIVGDVEGNARKIISAAHEAHAQGAALLLTPELALCGYAAEDLFLRPAFLDACDRALQDIAQATAACGD
LAVVVGHPWRSVAGGAERCHNAASVLRGGRVEQTYAKQELPNYAVFDEQRYFDPGTQPCVFDVQGVRVGLLICEDAWFPG
PAARAAQAGAQLLATLNASPFHLGKSAEREQIMRERVAETGLPLVYAHLVGGQDEVVFEGRSFALDATGFVGVRAPGFAE
ALVRVVARAEDGVLRFEPDVAPIPAPEADLWSALVLGVRDYVGKNGFPGVLLGLSGGIDSALVLAIAVDALGPDKVRAVM
MPSPYTADISWIDARDMAERLGVRYDEIAIAPQFEAFKAALASEFAGRAEDATEENLQARIRGTLLMALSNKFGSVVLTT
GNKSEMATGYCTLYGDMAGGFAVIKDVAKTTVYGLARWRNAHDPFGTGASPIPERIITRPPSAELRPDQKDQDSLPPYDV
LDAIVGRYMENDEPIESIIAAGYARADVERVTRLIQVNEYKRRQSPVGIRVTRRSFGKDWRYPITNRFRA
>Mature_549_residues
PFVISIAQFNFIVGDVEGNARKIISAAHEAHAQGAALLLTPELALCGYAAEDLFLRPAFLDACDRALQDIAQATAACGDL
AVVVGHPWRSVAGGAERCHNAASVLRGGRVEQTYAKQELPNYAVFDEQRYFDPGTQPCVFDVQGVRVGLLICEDAWFPGP
AARAAQAGAQLLATLNASPFHLGKSAEREQIMRERVAETGLPLVYAHLVGGQDEVVFEGRSFALDATGFVGVRAPGFAEA
LVRVVARAEDGVLRFEPDVAPIPAPEADLWSALVLGVRDYVGKNGFPGVLLGLSGGIDSALVLAIAVDALGPDKVRAVMM
PSPYTADISWIDARDMAERLGVRYDEIAIAPQFEAFKAALASEFAGRAEDATEENLQARIRGTLLMALSNKFGSVVLTTG
NKSEMATGYCTLYGDMAGGFAVIKDVAKTTVYGLARWRNAHDPFGTGASPIPERIITRPPSAELRPDQKDQDSLPPYDVL
DAIVGRYMENDEPIESIIAAGYARADVERVTRLIQVNEYKRRQSPVGIRVTRRSFGKDWRYPITNRFRA

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59422; Mature: 59291

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFVISIAQFNFIVGDVEGNARKIISAAHEAHAQGAALLLTPELALCGYAAEDLFLRPAF
CCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHEECHHH
LDACDRALQDIAQATAACGDLAVVVGHPWRSVAGGAERCHNAASVLRGGRVEQTYAKQEL
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHC
PNYAVFDEQRYFDPGTQPCVFDVQGVRVGLLICEDAWFPGPAARAAQAGAQLLATLNASP
CCCEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
FHLGKSAEREQIMRERVAETGLPLVYAHLVGGQDEVVFEGRSFALDATGFVGVRAPGFAE
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEEECCCEEEECCCCHHH
ALVRVVARAEDGVLRFEPDVAPIPAPEADLWSALVLGVRDYVGKNGFPGVLLGLSGGIDS
HHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
ALVLAIAVDALGPDKVRAVMMPSPYTADISWIDARDMAERLGVRYDEIAIAPQFEAFKAA
HHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCHHHHHHHHCCCCHHEEECCCHHHHHHH
LASEFAGRAEDATEENLQARIRGTLLMALSNKFGSVVLTTGNKSEMATGYCTLYGDMAGG
HHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCEEEEEEEECCCCH
FAVIKDVAKTTVYGLARWRNAHDPFGTGASPIPERIITRPPSAELRPDQKDQDSLPPYDV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHH
LDAIVGRYMENDEPIESIIAAGYARADVERVTRLIQVNEYKRRQSPVGIRVTRRSFGKDW
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCC
RYPITNRFRA
CCCCCCCCCC
>Mature Secondary Structure 
PFVISIAQFNFIVGDVEGNARKIISAAHEAHAQGAALLLTPELALCGYAAEDLFLRPAF
CEEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHEECHHH
LDACDRALQDIAQATAACGDLAVVVGHPWRSVAGGAERCHNAASVLRGGRVEQTYAKQEL
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHC
PNYAVFDEQRYFDPGTQPCVFDVQGVRVGLLICEDAWFPGPAARAAQAGAQLLATLNASP
CCCEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
FHLGKSAEREQIMRERVAETGLPLVYAHLVGGQDEVVFEGRSFALDATGFVGVRAPGFAE
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEEECCCEEEECCCCHHH
ALVRVVARAEDGVLRFEPDVAPIPAPEADLWSALVLGVRDYVGKNGFPGVLLGLSGGIDS
HHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
ALVLAIAVDALGPDKVRAVMMPSPYTADISWIDARDMAERLGVRYDEIAIAPQFEAFKAA
HHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCHHHHHHHHCCCCHHEEECCCHHHHHHH
LASEFAGRAEDATEENLQARIRGTLLMALSNKFGSVVLTTGNKSEMATGYCTLYGDMAGG
HHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCEEEEEEEECCCCH
FAVIKDVAKTTVYGLARWRNAHDPFGTGASPIPERIITRPPSAELRPDQKDQDSLPPYDV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHH
LDAIVGRYMENDEPIESIIAAGYARADVERVTRLIQVNEYKRRQSPVGIRVTRRSFGKDW
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCC
RYPITNRFRA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]