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The map label for this gene is gacS [H]

Identifier: 120611753

GI number: 120611753

Start: 3412195

End: 3413982

Strand: Direct

Name: gacS [H]

Synonym: Aave_3093

Alternate gene names: 120611753

Gene position: 3412195-3413982 (Clockwise)

Preceding gene: 120611752

Following gene: 120611756

Centisome position: 63.75

GC content: 69.24

Gene sequence:

>1788_bases
ATGCACCAGACATCCCTCCCCAGCACCACCGCAGAGCAGTCCCCGGCCCTGCCCGTGCACGGCGCGCATGGCAGCCGGCG
GCTGCGCGTTCTGGTGGTCGGCACCCTGCTGCTGACGCTCACGGTCACGTGGCTCCTGGTCGCCGTCTTGCTGAACACCA
AATGGCAGGACGCTGTGCGCTCCCAGGAGCGGCAGAACGCCAACCTGGCCCGGGTGCTGGAAGAGCAGACGCTCCGCGTC
CTGGCGGCCGCCGACCAGGCCACACTGCGCGTGGCGGCGGAAGTCCGCAGCGGCGAATTCAGCGAGCAGGACTACGCGCT
GTTCGCCAACGAGACCGGGCTGGCACCCGACATCCTCACCCAGTTGTCCATCGTGGGCCCCGACGGCCGTTTCGTCGGCA
GCAACCTCGATCCCTCGGCGGCCCGCAACCAGGACACCGACCTGAGCGAGCGCGAACATATCCGGGTGCACCTGGAGCCC
GGCAAGGAACCCAGGGCGCGGACGCGGATGTCCGCATCGGGACTGTTCGTCGGCAGGCCCGTGACCGGGCGGGTCTCCGG
CCGCCGGACCATGCAGTTGAGCCGGCCCATCGTCGGCAGCAACCGCTACCTGCACGGCGTGGTGGTCGCCTCGCTCAATC
CGGGCTACTTCGAGCAGGTGTACGGGAGCGTGGACATCGGCGCGCAGGGCGCGGTCGCGCTCATGGGCGCGGACTTCAAC
GTGCTGGCCCGGGTGATCGGAGCCCAGGCGGAAAGCATCGACAAGACCATCGAATTCCCGCCAGGCCATCCCCTCACCGA
CACCACCCGGACGCAGGGCACCTACCAGCGCGTCAGCCATATCGACGGACTGGGCCGCATCACCGCCTTCCATCGCGTGC
CGGGGTATCCGCTGACCGTCCTCGTCTCCACCACCGACGACGAGGCCCTGGCGGAATGGAAGACCATGCGGACCTTCACC
GTGGTGTTCGCCGGGCTTTTTTCCGCGACGCTGATCGCCCTCGGCGCGGGATTCCTGCGGGGGCTGCACCAGCTGGAGCA
GAAGAACCTGGCGCTAGCTGCGAGCGAGGCCCAGGCCCGTTCCGCCAACCGGGCCAAGAGCGAATTCCTGACGGCCATCT
CGCACGAACTGCGCACCCCCCTCACCAGCATCCACGGGTTCGCCGAACTCATGGAGCAGCGCCTGGAACAGCCGTCCTAC
CGCGAGGCCGCCGGCCTCATCCGCAGCGCGGCGGAACATCTCAACACGCTGCTGTCCGAGATCCTGGATCTGGCCAAGGT
CCAGGCCGGCGCCATGCCCATCGTGCCCGGGGACCATGCCGTGCGCGAACTCGTCGGCACCACCGTCGATCTCTTCGCCA
TCTCGGCAGTGCAGAAAGGGCTCACGCTGGAAATGCACATCGCTCCCGGCGTCCCGCAGGTACTGCGGTGCGACGGCCTG
CGGCTGAAGCAGATCCTCAACAACCTGCTGAGCAACGCGCTCAAGTTCACGCGGGAGGGGGGCGTGCGACTGGAGACGGA
CACCGCCGACGGCCACAGCGTGCGCTTCCGGGTCACCGATACCGGCCCCGGCATCCCCGCCGATCTGCACGAGGTCATCT
TCGAGAAATTCAGCCAGGGCAGCGCCGACGTGAGCACGCAGCACGGCGGCACCGGCCTGGGACTGGCGCTCTCGCGCGGA
CTGGCCGAGCTCATGGGCGGCCGCCTGACCGTGGCCTCGGAGCCGGGCCAGGGCGCCTGCTTCACGCTGGAGCTGCCGAT
GCACCCGCCGGCCGCCGACGGGGCCTGA

Upstream 100 bases:

>100_bases
GCGCCGCATCCTCGGGCACGGCGGCGGAAATGGCATGTTCGGCACGCCGTCGCCCCCCTGAGGCGTTCCCCACCGCACAG
GAACGTGGCAAGCTAGCCGC

Downstream 100 bases:

>100_bases
CGCCTCCGCACCTGCCCGCCCCCCCGGCCATGGCCGACCCCTTTTTCCTGGACGTCCCGGAAACAGAAAGGGCCGGCGCA
TGCGCGCCGGCCCCCGGACG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MHQTSLPSTTAEQSPALPVHGAHGSRRLRVLVVGTLLLTLTVTWLLVAVLLNTKWQDAVRSQERQNANLARVLEEQTLRV
LAAADQATLRVAAEVRSGEFSEQDYALFANETGLAPDILTQLSIVGPDGRFVGSNLDPSAARNQDTDLSEREHIRVHLEP
GKEPRARTRMSASGLFVGRPVTGRVSGRRTMQLSRPIVGSNRYLHGVVVASLNPGYFEQVYGSVDIGAQGAVALMGADFN
VLARVIGAQAESIDKTIEFPPGHPLTDTTRTQGTYQRVSHIDGLGRITAFHRVPGYPLTVLVSTTDDEALAEWKTMRTFT
VVFAGLFSATLIALGAGFLRGLHQLEQKNLALAASEAQARSANRAKSEFLTAISHELRTPLTSIHGFAELMEQRLEQPSY
REAAGLIRSAAEHLNTLLSEILDLAKVQAGAMPIVPGDHAVRELVGTTVDLFAISAVQKGLTLEMHIAPGVPQVLRCDGL
RLKQILNNLLSNALKFTREGGVRLETDTADGHSVRFRVTDTGPGIPADLHEVIFEKFSQGSADVSTQHGGTGLGLALSRG
LAELMGGRLTVASEPGQGACFTLELPMHPPAADGA

Sequences:

>Translated_595_residues
MHQTSLPSTTAEQSPALPVHGAHGSRRLRVLVVGTLLLTLTVTWLLVAVLLNTKWQDAVRSQERQNANLARVLEEQTLRV
LAAADQATLRVAAEVRSGEFSEQDYALFANETGLAPDILTQLSIVGPDGRFVGSNLDPSAARNQDTDLSEREHIRVHLEP
GKEPRARTRMSASGLFVGRPVTGRVSGRRTMQLSRPIVGSNRYLHGVVVASLNPGYFEQVYGSVDIGAQGAVALMGADFN
VLARVIGAQAESIDKTIEFPPGHPLTDTTRTQGTYQRVSHIDGLGRITAFHRVPGYPLTVLVSTTDDEALAEWKTMRTFT
VVFAGLFSATLIALGAGFLRGLHQLEQKNLALAASEAQARSANRAKSEFLTAISHELRTPLTSIHGFAELMEQRLEQPSY
REAAGLIRSAAEHLNTLLSEILDLAKVQAGAMPIVPGDHAVRELVGTTVDLFAISAVQKGLTLEMHIAPGVPQVLRCDGL
RLKQILNNLLSNALKFTREGGVRLETDTADGHSVRFRVTDTGPGIPADLHEVIFEKFSQGSADVSTQHGGTGLGLALSRG
LAELMGGRLTVASEPGQGACFTLELPMHPPAADGA
>Mature_595_residues
MHQTSLPSTTAEQSPALPVHGAHGSRRLRVLVVGTLLLTLTVTWLLVAVLLNTKWQDAVRSQERQNANLARVLEEQTLRV
LAAADQATLRVAAEVRSGEFSEQDYALFANETGLAPDILTQLSIVGPDGRFVGSNLDPSAARNQDTDLSEREHIRVHLEP
GKEPRARTRMSASGLFVGRPVTGRVSGRRTMQLSRPIVGSNRYLHGVVVASLNPGYFEQVYGSVDIGAQGAVALMGADFN
VLARVIGAQAESIDKTIEFPPGHPLTDTTRTQGTYQRVSHIDGLGRITAFHRVPGYPLTVLVSTTDDEALAEWKTMRTFT
VVFAGLFSATLIALGAGFLRGLHQLEQKNLALAASEAQARSANRAKSEFLTAISHELRTPLTSIHGFAELMEQRLEQPSY
REAAGLIRSAAEHLNTLLSEILDLAKVQAGAMPIVPGDHAVRELVGTTVDLFAISAVQKGLTLEMHIAPGVPQVLRCDGL
RLKQILNNLLSNALKFTREGGVRLETDTADGHSVRFRVTDTGPGIPADLHEVIFEKFSQGSADVSTQHGGTGLGLALSRG
LAELMGGRLTVASEPGQGACFTLELPMHPPAADGA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=244, Percent_Identity=35.655737704918, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI145693157, Length=230, Percent_Identity=37.3913043478261, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081816, Length=236, Percent_Identity=40.6779661016949, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=35.8870967741936, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=37.2384937238494, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=32.5301204819277, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=33.4821428571429, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=29.2682926829268, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87082128, Length=226, Percent_Identity=30.0884955752212, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=223, Percent_Identity=28.2511210762332, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786600, Length=238, Percent_Identity=29.8319327731092, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=30.6772908366534, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=263, Percent_Identity=28.1368821292776, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=249, Percent_Identity=28.9156626506024, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786783, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1789808, Length=308, Percent_Identity=24.6753246753247, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787894, Length=231, Percent_Identity=28.1385281385281, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=75, Percent_Identity=45.3333333333333, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63868; Mature: 63868

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQTSLPSTTAEQSPALPVHGAHGSRRLRVLVVGTLLLTLTVTWLLVAVLLNTKWQDAVR
CCCCCCCCCCCCCCCCCCEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SQERQNANLARVLEEQTLRVLAAADQATLRVAAEVRSGEFSEQDYALFANETGLAPDILT
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHH
QLSIVGPDGRFVGSNLDPSAARNQDTDLSEREHIRVHLEPGKEPRARTRMSASGLFVGRP
HEEEECCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCEEECCC
VTGRVSGRRTMQLSRPIVGSNRYLHGVVVASLNPGYFEQVYGSVDIGAQGAVALMGADFN
CCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCCEEEECCCHH
VLARVIGAQAESIDKTIEFPPGHPLTDTTRTQGTYQRVSHIDGLGRITAFHRVPGYPLTV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEE
LVSTTDDEALAEWKTMRTFTVVFAGLFSATLIALGAGFLRGLHQLEQKNLALAASEAQAR
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
SANRAKSEFLTAISHELRTPLTSIHGFAELMEQRLEQPSYREAAGLIRSAAEHLNTLLSE
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILDLAKVQAGAMPIVPGDHAVRELVGTTVDLFAISAVQKGLTLEMHIAPGVPQVLRCDGL
HHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCH
RLKQILNNLLSNALKFTREGGVRLETDTADGHSVRFRVTDTGPGIPADLHEVIFEKFSQG
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
SADVSTQHGGTGLGLALSRGLAELMGGRLTVASEPGQGACFTLELPMHPPAADGA
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MHQTSLPSTTAEQSPALPVHGAHGSRRLRVLVVGTLLLTLTVTWLLVAVLLNTKWQDAVR
CCCCCCCCCCCCCCCCCCEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SQERQNANLARVLEEQTLRVLAAADQATLRVAAEVRSGEFSEQDYALFANETGLAPDILT
HHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHH
QLSIVGPDGRFVGSNLDPSAARNQDTDLSEREHIRVHLEPGKEPRARTRMSASGLFVGRP
HEEEECCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCEEECCC
VTGRVSGRRTMQLSRPIVGSNRYLHGVVVASLNPGYFEQVYGSVDIGAQGAVALMGADFN
CCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCCEEEECCCHH
VLARVIGAQAESIDKTIEFPPGHPLTDTTRTQGTYQRVSHIDGLGRITAFHRVPGYPLTV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEE
LVSTTDDEALAEWKTMRTFTVVFAGLFSATLIALGAGFLRGLHQLEQKNLALAASEAQAR
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
SANRAKSEFLTAISHELRTPLTSIHGFAELMEQRLEQPSYREAAGLIRSAAEHLNTLLSE
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILDLAKVQAGAMPIVPGDHAVRELVGTTVDLFAISAVQKGLTLEMHIAPGVPQVLRCDGL
HHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCH
RLKQILNNLLSNALKFTREGGVRLETDTADGHSVRFRVTDTGPGIPADLHEVIFEKFSQG
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCC
SADVSTQHGGTGLGLALSRGLAELMGGRLTVASEPGQGACFTLELPMHPPAADGA
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]