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The map label for this gene is trmB

Identifier: 120611696

GI number: 120611696

Start: 3341705

End: 3342454

Strand: Direct

Name: trmB

Synonym: Aave_3033

Alternate gene names: 120611696

Gene position: 3341705-3342454 (Clockwise)

Preceding gene: 120611695

Following gene: 120611697

Centisome position: 62.43

GC content: 69.73

Gene sequence:

>750_bases
ATGCATTCCATCCCGGCCGATACCGGCCACACCCCTTCGCGCGCGCCTGCCGGCAACGGCTCGCCGCCCGCCGATGTTGC
CTTTCCCAAGGCGATCAAGAGCTACGTCCGCCGCGCCGGCCGCACCACCACCGGACAGGCCAAGGCCCTGGAGACCTTCG
GCTCGCAGTTCGTGCTCGACTACACCCCCGGGCCGCTCGACGCGCAAGCCGCTTTCGGCCGCGCCGCGCCACTCATCCTG
GAGATCGGCTTCGGCATGGGAGAAGCCACGGCCCACATCGCCGGCGTGCGGCCGCAGGACGACTTCCTGTGCTGCGAAGT
GCACGAGCCGGGCGTCGGCGCCCTGCTCAAGCGCATCGGCGAACGGGGCCTGACCAACATCCGCATCCTGCAGCACGACG
CCGTGGAGGTGATCGACCACATGCTCCCGGAAGGCAGCCTCGACGGCGTGCACATCTTCTTTCCGGATCCCTGGCACAAG
AAGCGCCACAACAAGCGGCGGCTGGTCCAGCCACCGCTCGTGGCCAAGCTCGCGCGGCGGCTGAAGCCCGGGGGCTACCT
GCACTGCGCGACCGACTGGCAACCCTATGCGGAGCAGATGCTGGAGGTGCTCTCGGCGGAGCCGCTGCTGGCCAACTCTG
CCGACGGCTACGCGCCGCAGCCCGACTACCGCCCGCTCACCAAGTTCGAGAACCGGGGCCTGCGCCTGGGCCACGGCGTG
TGGGATATCCTCTTCCGGCGCGTGGCCTGA

Upstream 100 bases:

>100_bases
TGCCGCCTCTGCAGCATCCGCACGACACCACCGTCGGCGAGGCGCTGGCGTGGTGGGCGCAGGCATGGCCGCGAATCTAC
AATCGGCCATCGTGACAGCC

Downstream 100 bases:

>100_bases
CAGGCAGCGCCGCGCATCCGTCGCAACGCCCGGCCCCCTTCCATGAAGCCCATCCTCGACAACCTGGTCGAAATGACCGG
CCATCGCGATCACCTGCGGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MHSIPADTGHTPSRAPAGNGSPPADVAFPKAIKSYVRRAGRTTTGQAKALETFGSQFVLDYTPGPLDAQAAFGRAAPLIL
EIGFGMGEATAHIAGVRPQDDFLCCEVHEPGVGALLKRIGERGLTNIRILQHDAVEVIDHMLPEGSLDGVHIFFPDPWHK
KRHNKRRLVQPPLVAKLARRLKPGGYLHCATDWQPYAEQMLEVLSAEPLLANSADGYAPQPDYRPLTKFENRGLRLGHGV
WDILFRRVA

Sequences:

>Translated_249_residues
MHSIPADTGHTPSRAPAGNGSPPADVAFPKAIKSYVRRAGRTTTGQAKALETFGSQFVLDYTPGPLDAQAAFGRAAPLIL
EIGFGMGEATAHIAGVRPQDDFLCCEVHEPGVGALLKRIGERGLTNIRILQHDAVEVIDHMLPEGSLDGVHIFFPDPWHK
KRHNKRRLVQPPLVAKLARRLKPGGYLHCATDWQPYAEQMLEVLSAEPLLANSADGYAPQPDYRPLTKFENRGLRLGHGV
WDILFRRVA
>Mature_249_residues
MHSIPADTGHTPSRAPAGNGSPPADVAFPKAIKSYVRRAGRTTTGQAKALETFGSQFVLDYTPGPLDAQAAFGRAAPLIL
EIGFGMGEATAHIAGVRPQDDFLCCEVHEPGVGALLKRIGERGLTNIRILQHDAVEVIDHMLPEGSLDGVHIFFPDPWHK
KRHNKRRLVQPPLVAKLARRLKPGGYLHCATDWQPYAEQMLEVLSAEPLLANSADGYAPQPDYRPLTKFENRGLRLGHGV
WDILFRRVA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=221, Percent_Identity=52.9411764705882, Blast_Score=241, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_ACIAC (A1TRL2)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971374.1
- ProteinModelPortal:   A1TRL2
- SMR:   A1TRL2
- STRING:   A1TRL2
- GeneID:   4666851
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3033
- NMPDR:   fig|397945.5.peg.2582
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   A1TRL2
- ProtClustDB:   PRK00121
- BioCyc:   AAVE397945:AAVE_3033-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27271; Mature: 27271

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: BINDING 81-81 BINDING 106-106 BINDING 133-133 BINDING 156-156 BINDING 160-160 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSIPADTGHTPSRAPAGNGSPPADVAFPKAIKSYVRRAGRTTTGQAKALETFGSQFVLD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEE
YTPGPLDAQAAFGRAAPLILEIGFGMGEATAHIAGVRPQDDFLCCEVHEPGVGALLKRIG
CCCCCCCHHHHHCCCCCEEEEECCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHH
ERGLTNIRILQHDAVEVIDHMLPEGSLDGVHIFFPDPWHKKRHNKRRLVQPPLVAKLARR
HCCCCEEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHH
LKPGGYLHCATDWQPYAEQMLEVLSAEPLLANSADGYAPQPDYRPLTKFENRGLRLGHGV
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHH
WDILFRRVA
HHHHHHHCC
>Mature Secondary Structure
MHSIPADTGHTPSRAPAGNGSPPADVAFPKAIKSYVRRAGRTTTGQAKALETFGSQFVLD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEE
YTPGPLDAQAAFGRAAPLILEIGFGMGEATAHIAGVRPQDDFLCCEVHEPGVGALLKRIG
CCCCCCCHHHHHCCCCCEEEEECCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHH
ERGLTNIRILQHDAVEVIDHMLPEGSLDGVHIFFPDPWHKKRHNKRRLVQPPLVAKLARR
HCCCCEEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHH
LKPGGYLHCATDWQPYAEQMLEVLSAEPLLANSADGYAPQPDYRPLTKFENRGLRLGHGV
CCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHH
WDILFRRVA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA