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The map label for this gene is ycdT [H]

Identifier: 120611697

GI number: 120611697

Start: 3342497

End: 3343540

Strand: Direct

Name: ycdT [H]

Synonym: Aave_3034

Alternate gene names: 120611697

Gene position: 3342497-3343540 (Clockwise)

Preceding gene: 120611696

Following gene: 120611701

Centisome position: 62.44

GC content: 67.24

Gene sequence:

>1044_bases
ATGAAGCCCATCCTCGACAACCTGGTCGAAATGACCGGCCATCGCGATCACCTGCGGCTGGAAGTGTCGGTCCTGTCCAC
GCTGCACAAGCTTCCTTCCATCAAGGCGGTGCGCGCGCTGGAGATCTTCGAGTCGGGGGGCGAAGCCTGCGTGCGGCCGC
GCACCTGGCACCAGGACGGGCAGATGGTCACCAGCGACTGGGAAGCCCCGGTCGATCCGCGGCGCGAACCGCTGTCGGCA
TTCCCGGCGCTGCATGAATGCATCGCGCAGCGCCGGCCGCACGTGCTGCAGTCCGTGCGGCGCGGCGAGCACATGCTGTG
GCTGCCGGTGTGGCTGCACGATTCGGTGCATGCCTGCATCGAAGTCACGCAGTCGCGGCCCTTCTCGGCCCACAAGCTCG
ACGTGATCCAGAGCGTCTTCTTCGTCTATCAGAACTACCAGAGCCTGCTGGACTACAGCGAGCGCGACGCGCTGACCGGC
CTCTTCAACCGCAAGACCTTCGACGAGCAGTTCGCCCGGCGCGGCGGCGTGTCCAGCAACGCGGCGAGACAGGCGCCCAC
CGAGCCGGCCGATGCGTCCGAGCCGCGCGGCGAGGCGCCGCAGCACTGGCTGGCCGTGGTGGACATCGACCACTTCAAGC
AGGTCAACGACCGCTTCGGTCACCTGTACGGCGACGAGGTGCTGATCCTCGTCGCCAACATCCTGCGCAACTCCTTCCGC
GGGCACGACCGCATCTTCCGCTTCGGCGGGGAGGAGTTCGTGGTTCTGCTGCGCTCGACGACGCTCGCCACCGCCCACAA
GGCCTTCAACCGGTTCCGCATCGCCGTGCAGGAATACGACTTCCCGCAGGTGGGCCGCGTCACGGTGAGCCTGGGCTTCG
CGAGCACGGAGCGCGGCGCCCCGGTGGAGATCCTGGGCCAGGCGGACCAGGCGCTCTACTACGCCAAGGAACACGGCCGC
AACCGCGTGTGCTACTACGACGACCTGGTGCGCGAGGGCCATCTGCACGCCAAGGTCGCGAACGACAACGTCGAACTGTT
CTGA

Upstream 100 bases:

>100_bases
CCGGGGCCTGCGCCTGGGCCACGGCGTGTGGGATATCCTCTTCCGGCGCGTGGCCTGACAGGCAGCGCCGCGCATCCGTC
GCAACGCCCGGCCCCCTTCC

Downstream 100 bases:

>100_bases
CCGGGCCGGCCGGGGCAGTGTTCCGGCCCACGCCCCGCTGCACTCCAGCTGTCTCTTGAATGCAAAAAGGCCCGCGCAGG
CGGGCCTTCTGCTCTGGGCT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKPILDNLVEMTGHRDHLRLEVSVLSTLHKLPSIKAVRALEIFESGGEACVRPRTWHQDGQMVTSDWEAPVDPRREPLSA
FPALHECIAQRRPHVLQSVRRGEHMLWLPVWLHDSVHACIEVTQSRPFSAHKLDVIQSVFFVYQNYQSLLDYSERDALTG
LFNRKTFDEQFARRGGVSSNAARQAPTEPADASEPRGEAPQHWLAVVDIDHFKQVNDRFGHLYGDEVLILVANILRNSFR
GHDRIFRFGGEEFVVLLRSTTLATAHKAFNRFRIAVQEYDFPQVGRVTVSLGFASTERGAPVEILGQADQALYYAKEHGR
NRVCYYDDLVREGHLHAKVANDNVELF

Sequences:

>Translated_347_residues
MKPILDNLVEMTGHRDHLRLEVSVLSTLHKLPSIKAVRALEIFESGGEACVRPRTWHQDGQMVTSDWEAPVDPRREPLSA
FPALHECIAQRRPHVLQSVRRGEHMLWLPVWLHDSVHACIEVTQSRPFSAHKLDVIQSVFFVYQNYQSLLDYSERDALTG
LFNRKTFDEQFARRGGVSSNAARQAPTEPADASEPRGEAPQHWLAVVDIDHFKQVNDRFGHLYGDEVLILVANILRNSFR
GHDRIFRFGGEEFVVLLRSTTLATAHKAFNRFRIAVQEYDFPQVGRVTVSLGFASTERGAPVEILGQADQALYYAKEHGR
NRVCYYDDLVREGHLHAKVANDNVELF
>Mature_347_residues
MKPILDNLVEMTGHRDHLRLEVSVLSTLHKLPSIKAVRALEIFESGGEACVRPRTWHQDGQMVTSDWEAPVDPRREPLSA
FPALHECIAQRRPHVLQSVRRGEHMLWLPVWLHDSVHACIEVTQSRPFSAHKLDVIQSVFFVYQNYQSLLDYSERDALTG
LFNRKTFDEQFARRGGVSSNAARQAPTEPADASEPRGEAPQHWLAVVDIDHFKQVNDRFGHLYGDEVLILVANILRNSFR
GHDRIFRFGGEEFVVLLRSTTLATAHKAFNRFRIAVQEYDFPQVGRVTVSLGFASTERGAPVEILGQADQALYYAKEHGR
NRVCYYDDLVREGHLHAKVANDNVELF

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=186, Percent_Identity=38.1720430107527, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=38.4615384615385, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1786584, Length=202, Percent_Identity=31.6831683168317, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082007, Length=129, Percent_Identity=39.5348837209302, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787816, Length=181, Percent_Identity=31.4917127071823, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=198, Percent_Identity=31.3131313131313, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=31.7919075144509, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787056, Length=183, Percent_Identity=30.6010928961749, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=204, Percent_Identity=26.9607843137255, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787541, Length=131, Percent_Identity=35.8778625954198, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39584; Mature: 39584

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPILDNLVEMTGHRDHLRLEVSVLSTLHKLPSIKAVRALEIFESGGEACVRPRTWHQDG
CCHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCCCCC
QMVTSDWEAPVDPRREPLSAFPALHECIAQRRPHVLQSVRRGEHMLWLPVWLHDSVHACI
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEHHHHHHHHHH
EVTQSRPFSAHKLDVIQSVFFVYQNYQSLLDYSERDALTGLFNRKTFDEQFARRGGVSSN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCC
AARQAPTEPADASEPRGEAPQHWLAVVDIDHFKQVNDRFGHLYGDEVLILVANILRNSFR
CCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GHDRIFRFGGEEFVVLLRSTTLATAHKAFNRFRIAVQEYDFPQVGRVTVSLGFASTERGA
CCHHHEEECCCEEEEEEEHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEECCCCCCC
PVEILGQADQALYYAKEHGRNRVCYYDDLVREGHLHAKVANDNVELF
CEEEECCCHHHHHHHHHCCCCCEEEHHHHHHCCCCEEEEECCCCEEC
>Mature Secondary Structure
MKPILDNLVEMTGHRDHLRLEVSVLSTLHKLPSIKAVRALEIFESGGEACVRPRTWHQDG
CCHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCCCCC
QMVTSDWEAPVDPRREPLSAFPALHECIAQRRPHVLQSVRRGEHMLWLPVWLHDSVHACI
CEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEHHHHHHHHHH
EVTQSRPFSAHKLDVIQSVFFVYQNYQSLLDYSERDALTGLFNRKTFDEQFARRGGVSSN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCC
AARQAPTEPADASEPRGEAPQHWLAVVDIDHFKQVNDRFGHLYGDEVLILVANILRNSFR
CCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GHDRIFRFGGEEFVVLLRSTTLATAHKAFNRFRIAVQEYDFPQVGRVTVSLGFASTERGA
CCHHHEEECCCEEEEEEEHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEECCCCCCC
PVEILGQADQALYYAKEHGRNRVCYYDDLVREGHLHAKVANDNVELF
CEEEECCCHHHHHHHHHCCCCCEEEHHHHHHCCCCEEEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]