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The map label for this gene is ligA

Identifier: 120611505

GI number: 120611505

Start: 3125701

End: 3127899

Strand: Reverse

Name: ligA

Synonym: Aave_2841

Alternate gene names: 120611505

Gene position: 3127899-3125701 (Counterclockwise)

Preceding gene: 120611506

Following gene: 120611504

Centisome position: 58.44

GC content: 70.4

Gene sequence:

>2199_bases
ATGGCCGAACATCCCGACCTGTTTTCCGCCCCTGCCCAGGAGGCCCCTGAAGACCTGGGCCTGCGCGCCGCCGCGCTGCG
GGCGCAGTTGCACCAATGGGCACACCAGTACTACGTGCTGGACGCACCCACCGTGCCGGATGCCGAATACGACCGCGTCT
TCCAGGCCCTGCAGGCACTGGAGACGGCGCACCCCGAGCTCGTGACACCCGATTCGCCCACGCAGCGCGTGATCGGCGCG
GTGATGGAGGGCCTGACGCCCGTTCGCCACACGGTGCCGATGCTCAGCATCCGCACCGAGACCGACACCGAGGCCAGCGG
CGCCGAGACGTTCGATGCCCGCGTCCGCCGGGAGCTCAAGCTCGCGCCCGACGCACCGCCGGTCGAATACGTCGCCGAGC
CCAAATTCGACGGCCTGGCCATGAGCCTGCGCTATGAGAACGGTCGCCTGGTACAGGCCGCCACGCGCGGCGACGGCGAG
GTGGGCGAGGACGTGACCCACAACATCCGTACCATCCGCCAGATTCCCCTCACGCTGCCCACGGGCGGCCGCTATGGCGT
GCCGCCCGTACTGGAGGTGCGGGGCGAGGTCTACATGCGCCGGGCGGATTTCGACCGGCTCAACGAGCGCCAGCGCGAGG
CCGGCGGCAAGACCTTCGTCAACCCGCGCAATGCGGCGGCCGGCGCGGTGCGCCAGCTCGACTCGGGCATTGCCGCGCAG
CGGCCCCTGAGCTTCTTCGCCTATGGCCTGGGCGACATCACTCCGGCAGCAGAAGGCGGCCCCGACTTCGCGACCCATTT
CGACATGCTGCGGCAGCTCAAGGCCTGGGGCTTCCCGGTCGCCGCGCAGGTGCGGACCGCGCGGGGCGCGTCCGAGCTGG
TCGCTTTCCACCAGGAGGTGGGCGCGAGCCGCGACCAGTTGCCCTACGACATCGACGGGGTGGTGTACAAGGTCAACAGC
CTGGCGCTGCAGCGCCAGCTGGGATTCGTGACCCGCGAGCCGCGGTGGGCCGTGGCGCACAAGTACCCGGCGCAGGAGAT
GGTCACCCGCGTGGAGGGTATCGACGTACAGGTGGGCCGCACCGGCAAGCTCACCCCCGTGGCGCGGCTGGCTCCCGTCT
TCGTCGGTGGCGTCACCGTCACCAACGCCACGCTGCACAACCTGTTCGAGATCCGAAAGAAGGGCGTGCGCGTGGGCGAC
CAGGTCATCGTGCGCCGCGCGGGCGACGTGATCCCCGAAGTGGTGGGCACGGTTCCTGCGGCCCTGCTGCCCGTGGCGGG
CGCGCTGCAGGGCTCCGATGCCCTGGCGGACGCCGCTTCCGGGGCCGACGGCACGGCGCCGGGCGCCGACGCGGCGCGTG
CCGCGCCGCGGTCGCCCTACGTGCCGAACTTCCGCATGCCGCGCCAGTGCCCCATCTGCGGCAGTACGGTGGTCCGGGAA
AAAGGCGAAGCCAACCATCGCTGCACGGGGGGGCTCTTCTGCCCGGCCCAGCGCAAGGAGGCGCTGCTGCACTTCGCCCA
GCGCCGGGCCATGGACATCGAAGGCCTGGGCGAAAAGCTGGTGGACCAGCTGGTGGAGGGCCAGGTCATCCGTACCCTGC
CGGACCTCTACCGCCTGGGCCTGACGGCGCTCAGCAGCCTGGACCGCATGGCGGAGAAATCCGCGCAGAACGTGCTGGCG
GCCCTGGAAAAGTCCAAGCACACCACGCTGCCGCGCTTCCTGTTCGGCCTGGGCATCCGCCATGTAGGCGAAGCCACGGC
CAAGGACCTGGCCCGCCATTTCGGCGGCCTCGATGCCATCATGGACGCCAGCGTGGAGCAACTGCTGGAGGTGAACGACG
TCGGCCCCGTCGTGGCGGAGGCCATCCACACGTTCTTCGCGCAGCCCCACAACCGCGAGGTGGTCGAGCAGCTGCGGGCC
TGCGGCGTCACCTGGAAGGAAGGCCCGCCTGCAGAACGCGCCACCTTGCCGCTGGCCGGGAAAACCTTTGTTCTCACCGG
CACGCTGCCGACTCTGAGCCGTGAAGACGCCAAGGACCGGCTCGAAGCCGCGGGCGCGAAGGTCGCAGGCTCCGTCAGCA
GGAAAACGCACTACGTCGTGGCAGGCGAGGAGGCCGGCAGCAAGCTGGCCAAGGCACAGGAGCTGGGCGTGCCGGTACTG
GACGAGGCGGGCATGCTCGCCCTCCTGCAGGGCCGCTGA

Upstream 100 bases:

>100_bases
TGTCTGCCGGATCGGTGCTGGCGCGGCGGCTGTTCAGCTGAACATCGCACGGCGGCCCGGCGGCGGCCCGCCGTGCCCCC
TTTCCCGAAAGACGATCGCC

Downstream 100 bases:

>100_bases
GCCAGGGCGCGGAAACGGCGTGGCAGCGGGTTGGGACCCGCTGCATTTCAGGGCGAGGAGGTCGCATGCTGCCAGCGTCT
GCGGCGGAGCAGCCTGCGTG

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 732; Mature: 731

Protein sequence:

>732_residues
MAEHPDLFSAPAQEAPEDLGLRAAALRAQLHQWAHQYYVLDAPTVPDAEYDRVFQALQALETAHPELVTPDSPTQRVIGA
VMEGLTPVRHTVPMLSIRTETDTEASGAETFDARVRRELKLAPDAPPVEYVAEPKFDGLAMSLRYENGRLVQAATRGDGE
VGEDVTHNIRTIRQIPLTLPTGGRYGVPPVLEVRGEVYMRRADFDRLNERQREAGGKTFVNPRNAAAGAVRQLDSGIAAQ
RPLSFFAYGLGDITPAAEGGPDFATHFDMLRQLKAWGFPVAAQVRTARGASELVAFHQEVGASRDQLPYDIDGVVYKVNS
LALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFEIRKKGVRVGD
QVIVRRAGDVIPEVVGTVPAALLPVAGALQGSDALADAASGADGTAPGADAARAAPRSPYVPNFRMPRQCPICGSTVVRE
KGEANHRCTGGLFCPAQRKEALLHFAQRRAMDIEGLGEKLVDQLVEGQVIRTLPDLYRLGLTALSSLDRMAEKSAQNVLA
ALEKSKHTTLPRFLFGLGIRHVGEATAKDLARHFGGLDAIMDASVEQLLEVNDVGPVVAEAIHTFFAQPHNREVVEQLRA
CGVTWKEGPPAERATLPLAGKTFVLTGTLPTLSREDAKDRLEAAGAKVAGSVSRKTHYVVAGEEAGSKLAKAQELGVPVL
DEAGMLALLQGR

Sequences:

>Translated_732_residues
MAEHPDLFSAPAQEAPEDLGLRAAALRAQLHQWAHQYYVLDAPTVPDAEYDRVFQALQALETAHPELVTPDSPTQRVIGA
VMEGLTPVRHTVPMLSIRTETDTEASGAETFDARVRRELKLAPDAPPVEYVAEPKFDGLAMSLRYENGRLVQAATRGDGE
VGEDVTHNIRTIRQIPLTLPTGGRYGVPPVLEVRGEVYMRRADFDRLNERQREAGGKTFVNPRNAAAGAVRQLDSGIAAQ
RPLSFFAYGLGDITPAAEGGPDFATHFDMLRQLKAWGFPVAAQVRTARGASELVAFHQEVGASRDQLPYDIDGVVYKVNS
LALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFEIRKKGVRVGD
QVIVRRAGDVIPEVVGTVPAALLPVAGALQGSDALADAASGADGTAPGADAARAAPRSPYVPNFRMPRQCPICGSTVVRE
KGEANHRCTGGLFCPAQRKEALLHFAQRRAMDIEGLGEKLVDQLVEGQVIRTLPDLYRLGLTALSSLDRMAEKSAQNVLA
ALEKSKHTTLPRFLFGLGIRHVGEATAKDLARHFGGLDAIMDASVEQLLEVNDVGPVVAEAIHTFFAQPHNREVVEQLRA
CGVTWKEGPPAERATLPLAGKTFVLTGTLPTLSREDAKDRLEAAGAKVAGSVSRKTHYVVAGEEAGSKLAKAQELGVPVL
DEAGMLALLQGR
>Mature_731_residues
AEHPDLFSAPAQEAPEDLGLRAAALRAQLHQWAHQYYVLDAPTVPDAEYDRVFQALQALETAHPELVTPDSPTQRVIGAV
MEGLTPVRHTVPMLSIRTETDTEASGAETFDARVRRELKLAPDAPPVEYVAEPKFDGLAMSLRYENGRLVQAATRGDGEV
GEDVTHNIRTIRQIPLTLPTGGRYGVPPVLEVRGEVYMRRADFDRLNERQREAGGKTFVNPRNAAAGAVRQLDSGIAAQR
PLSFFAYGLGDITPAAEGGPDFATHFDMLRQLKAWGFPVAAQVRTARGASELVAFHQEVGASRDQLPYDIDGVVYKVNSL
ALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFEIRKKGVRVGDQ
VIVRRAGDVIPEVVGTVPAALLPVAGALQGSDALADAASGADGTAPGADAARAAPRSPYVPNFRMPRQCPICGSTVVREK
GEANHRCTGGLFCPAQRKEALLHFAQRRAMDIEGLGEKLVDQLVEGQVIRTLPDLYRLGLTALSSLDRMAEKSAQNVLAA
LEKSKHTTLPRFLFGLGIRHVGEATAKDLARHFGGLDAIMDASVEQLLEVNDVGPVVAEAIHTFFAQPHNREVVEQLRAC
GVTWKEGPPAERATLPLAGKTFVLTGTLPTLSREDAKDRLEAAGAKVAGSVSRKTHYVVAGEEAGSKLAKAQELGVPVLD
EAGMLALLQGR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=74, Percent_Identity=45.945945945946, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788750, Length=707, Percent_Identity=52.7581329561528, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=395, Percent_Identity=27.5949367088608, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ACIAC (A1TR21)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971183.1
- ProteinModelPortal:   A1TR21
- STRING:   A1TR21
- GeneID:   4667429
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2841
- NMPDR:   fig|397945.5.peg.2415
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A1TR21
- ProtClustDB:   CLSK951442
- BioCyc:   AAVE397945:AAVE_2841-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 78898; Mature: 78767

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 135-135 BINDING 133-133 BINDING 156-156 BINDING 196-196 BINDING 317-317 BINDING 341-341

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHPDLFSAPAQEAPEDLGLRAAALRAQLHQWAHQYYVLDAPTVPDAEYDRVFQALQAL
CCCCCCCCCCCHHCCCHHHCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHH
ETAHPELVTPDSPTQRVIGAVMEGLTPVRHTVPMLSIRTETDTEASGAETFDARVRRELK
HHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHC
LAPDAPPVEYVAEPKFDGLAMSLRYENGRLVQAATRGDGEVGEDVTHNIRTIRQIPLTLP
CCCCCCCHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEECC
TGGRYGVPPVLEVRGEVYMRRADFDRLNERQREAGGKTFVNPRNAAAGAVRQLDSGIAAQ
CCCCCCCCHHHHHCCHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHH
RPLSFFAYGLGDITPAAEGGPDFATHFDMLRQLKAWGFPVAAQVRTARGASELVAFHQEV
CCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
GASRDQLPYDIDGVVYKVNSLALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGR
CCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEHHCCCCHHHHHHHHCCCEEEECC
TGKLTPVARLAPVFVGGVTVTNATLHNLFEIRKKGVRVGDQVIVRRAGDVIPEVVGTVPA
CCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ALLPVAGALQGSDALADAASGADGTAPGADAARAAPRSPYVPNFRMPRQCPICGSTVVRE
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHC
KGEANHRCTGGLFCPAQRKEALLHFAQRRAMDIEGLGEKLVDQLVEGQVIRTLPDLYRLG
CCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LTALSSLDRMAEKSAQNVLAALEKSKHTTLPRFLFGLGIRHVGEATAKDLARHFGGLDAI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MDASVEQLLEVNDVGPVVAEAIHTFFAQPHNREVVEQLRACGVTWKEGPPAERATLPLAG
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KTFVLTGTLPTLSREDAKDRLEAAGAKVAGSVSRKTHYVVAGEEAGSKLAKAQELGVPVL
CEEEEECCCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEE
DEAGMLALLQGR
CCCCCEEEECCC
>Mature Secondary Structure 
AEHPDLFSAPAQEAPEDLGLRAAALRAQLHQWAHQYYVLDAPTVPDAEYDRVFQALQAL
CCCCCCCCCCHHCCCHHHCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHH
ETAHPELVTPDSPTQRVIGAVMEGLTPVRHTVPMLSIRTETDTEASGAETFDARVRRELK
HHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHC
LAPDAPPVEYVAEPKFDGLAMSLRYENGRLVQAATRGDGEVGEDVTHNIRTIRQIPLTLP
CCCCCCCHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEECC
TGGRYGVPPVLEVRGEVYMRRADFDRLNERQREAGGKTFVNPRNAAAGAVRQLDSGIAAQ
CCCCCCCCHHHHHCCHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHH
RPLSFFAYGLGDITPAAEGGPDFATHFDMLRQLKAWGFPVAAQVRTARGASELVAFHQEV
CCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
GASRDQLPYDIDGVVYKVNSLALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGR
CCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEHHCCCCHHHHHHHHCCCEEEECC
TGKLTPVARLAPVFVGGVTVTNATLHNLFEIRKKGVRVGDQVIVRRAGDVIPEVVGTVPA
CCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ALLPVAGALQGSDALADAASGADGTAPGADAARAAPRSPYVPNFRMPRQCPICGSTVVRE
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHC
KGEANHRCTGGLFCPAQRKEALLHFAQRRAMDIEGLGEKLVDQLVEGQVIRTLPDLYRLG
CCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LTALSSLDRMAEKSAQNVLAALEKSKHTTLPRFLFGLGIRHVGEATAKDLARHFGGLDAI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MDASVEQLLEVNDVGPVVAEAIHTFFAQPHNREVVEQLRACGVTWKEGPPAERATLPLAG
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KTFVLTGTLPTLSREDAKDRLEAAGAKVAGSVSRKTHYVVAGEEAGSKLAKAQELGVPVL
CEEEEECCCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEE
DEAGMLALLQGR
CCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA