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The map label for this gene is nasD [H]

Identifier: 120610784

GI number: 120610784

Start: 2310788

End: 2313259

Strand: Reverse

Name: nasD [H]

Synonym: Aave_2107

Alternate gene names: 120610784

Gene position: 2313259-2310788 (Counterclockwise)

Preceding gene: 120610785

Following gene: 120610783

Centisome position: 43.22

GC content: 67.15

Gene sequence:

>2472_bases
ATGAAGAAATCCAGATTGGTGATGGTGGGCAACGGCATGGCCGGCGTGCGCACGCTCGAGGAGCTGCTGAAGATCGCGCC
CGATCTGTACGAGATCACCGTCTTCGGCGCCGAACCGCACCCCAACTACAACCGCATCCTGCTGTCGCCCGTGCTCGCGG
GCGAGCAGACGCTGGAGGAGATCGTCCTCAACGACTGGTCCTGGTACGAGGACAACGGCATCACGCTGCACGCGGGCTGC
ACCGTGCGCGAGGTGGATCGTACCCGGCGCATCGTGCACGGCACGGACGCCCAGGGCCGCACCATCGAGGCGCCCTACGA
CCGGCTGATCCTCGCCACCGGCTCCAATCCCTTCATCCTGCCCATTCCCGGCAATCAGCTTTCGGGCGTGCTGGCCTACC
GCGACATCGCCGACACCCAGGCCATGATCGATGCGGCCGCCATCTACCGCGACGCGGTCGTGATCGGCGGAGGACTGCTC
GGGCTGGAGGCCGCCAACGGCCTCATGAAGCGCGGCATGCGCGTGACCGTGGTGCATGCGAGCGAGTGGCTCATGGAGCG
CCAGCTCGACGACGTGGCCGGCCGCATGCTGCAGAAATCCCTGGCGGAGCGCGGCATGGGCTTCCTCATGAAGGCGCAGA
CCCGGGAGCTGGTGGGCGATGCCTCGGGCCGCGTGGCCGCCGTGCGCTTCCAGGACGGCACGGAGGTGCCTGCCCAGCTC
GTCGTCATGGCCGTGGGCATCCGCCCGAGCACGCAGCTCGCCGAGAGCATGCGCCTGCACGTGAACCGCGGCATCGTGGT
GAGCGACACGCTGCAGACGGTGACCGATGCCCGCATCTACGCGGTGGGCGAATGCGCCGCGCACCGCGGCATCGCCTACG
GCCTCGTGGCGCCGCTCTTCGAGCAGGGCAAGGTGCTGGCCAACCACCTGGCCGAGTACGGCATCGCGCGCTACCAGGGC
TCGCTCACCTCCACCAAGCTCAAGGTGACCGGCATCGACCTGTTTTCCGCCGGCAACTTCCAGGGCGGCGAGGGCACGGA
GGAAATCGTGATGAGCGATCCCTTCGGCGGGGTGTACAAGAAGCTGGTGATCCGCGACGACCAACTCGTGGGTGCCTGTC
TCTACGGCGACACCGTGGACGGCAGCTGGTACTTCAAGCTGCTGCGCGAGGGCCGCTCCATCGGAGATATCCGCGACAAG
CTGATGTTCGGCGAATCCCACCTCGGCGACACCGGCCACCAGGGCCAGAGCAAGGCCGCCGCCATGCAGGATACCGACGA
GGTCTGCGGCTGCAATGGCGTGACCAAGGGCGCGATCTGCAAGGCGATCAAGGACAAGGGCCTGTTCACGCTGGAGGACG
TGCGCAAGCACACCAAGGCCAGCGCGAGCTGCGGCTCCTGCACCGGCCTCGTGGAGCAGATCATCATGTTCACCGCGGGC
GGCGACTACTCCGCCACGCCCCGGACCAAGGCCGTCTGCGGCTGCACCGACCACGGCCACCAGGCCGTGCGCGACGCGAT
CCGCACCCACCGCCTGCTCACCATCCAGGACGTTTTCAAATTCCTGGAGTGGAAGACGCCCGATGGCTGCCCCACCTGCC
GGCCCGCGGTGAACTACTACCTGATCAGCACCTGGCCCAAGGAAGCCAGGGACGACCCGCAGAGCCGCGCCATCAACGAG
CGCTCGCACGCCAACATCCAGAAGGACGGCACGTACAGCGTGGTTCCGCGCATGTGGGGCGGCGAAACCACCGCCGACGA
ACTGCGCCGCATCGCCGATGCCGTGGACAAATACAACATCCCCACCGTGAAGGTCACGGGCGGCCAGCGCATCGACCTGC
TCGGCGTGAAGAAGGAAGACCTCGTCAACGTCTGGAAGGACATCGGCATGCCCAGCGGCCACGCCTACGCCAAGGCGCTG
CGCACGGTGAAGACCTGCGTGGGCAGCGAATGGTGCCGCATGGGCACGCAGGACAGCACGCAGATGGGCAAGGACCTGGA
GCGCGCGATGTGGCGCATGTACGCCCCCCACAAGGTCAAGTTCGCCGTGAGCGGCTGCCCGCGCAACTGCGCCGAGGCCG
GCATCAAGGACGTGGGCATCATCGGCGTGGACTCCGGCTGGGAGATGTACATCGCCGGCAACGGCGGCATCAAGACCGAG
GTCGCCCACTTCTTCACCAAGCTCAAGACCGCCGAGGAAGTGCTCGAATACACGGGCGCCTTCTGCGAGCTCTATCGCCA
GGAGGGCTGGTACCTGGAGCGCACCGTGCACTACGTGAACCGCGTGGGCCTGGACTACGTGAAGAAGCGCATCCTCGAAG
ACCACGCAGGCCGCAAGGCGCTCTGGGCCCAGCTGCAGTTCGCGCTCGACGGCGAGCCCGACCCCTGGTTCGAGTTCGAC
AAGGCCGCGGTGGATACCCGGCAGTTCGTCCCGCTGGCGCCTTCCGCTGCTGCAGCCGAAGCCACCCGCTGA

Upstream 100 bases:

>100_bases
CAGGACGAAGGCGTCCCCACCCGTGCGAGCGGCCCGCTATTGCGGCGCATGCAGGGTGCGGACGCCTTTTTTGTTTTCCG
AACCGCCCGTGGGGGTCACG

Downstream 100 bases:

>100_bases
TTCCTGACCGTCCCGATCCTTCCCCGAATTCCCACGAAACCCGCGAGAGGCCCCCGATGACCCAATGGACCCCCATCTGC
CGCGTGGACGACATCCCCGT

Product: assimilatory nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 823; Mature: 823

Protein sequence:

>823_residues
MKKSRLVMVGNGMAGVRTLEELLKIAPDLYEITVFGAEPHPNYNRILLSPVLAGEQTLEEIVLNDWSWYEDNGITLHAGC
TVREVDRTRRIVHGTDAQGRTIEAPYDRLILATGSNPFILPIPGNQLSGVLAYRDIADTQAMIDAAAIYRDAVVIGGGLL
GLEAANGLMKRGMRVTVVHASEWLMERQLDDVAGRMLQKSLAERGMGFLMKAQTRELVGDASGRVAAVRFQDGTEVPAQL
VVMAVGIRPSTQLAESMRLHVNRGIVVSDTLQTVTDARIYAVGECAAHRGIAYGLVAPLFEQGKVLANHLAEYGIARYQG
SLTSTKLKVTGIDLFSAGNFQGGEGTEEIVMSDPFGGVYKKLVIRDDQLVGACLYGDTVDGSWYFKLLREGRSIGDIRDK
LMFGESHLGDTGHQGQSKAAAMQDTDEVCGCNGVTKGAICKAIKDKGLFTLEDVRKHTKASASCGSCTGLVEQIIMFTAG
GDYSATPRTKAVCGCTDHGHQAVRDAIRTHRLLTIQDVFKFLEWKTPDGCPTCRPAVNYYLISTWPKEARDDPQSRAINE
RSHANIQKDGTYSVVPRMWGGETTADELRRIADAVDKYNIPTVKVTGGQRIDLLGVKKEDLVNVWKDIGMPSGHAYAKAL
RTVKTCVGSEWCRMGTQDSTQMGKDLERAMWRMYAPHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIAGNGGIKTE
VAHFFTKLKTAEEVLEYTGAFCELYRQEGWYLERTVHYVNRVGLDYVKKRILEDHAGRKALWAQLQFALDGEPDPWFEFD
KAAVDTRQFVPLAPSAAAAEATR

Sequences:

>Translated_823_residues
MKKSRLVMVGNGMAGVRTLEELLKIAPDLYEITVFGAEPHPNYNRILLSPVLAGEQTLEEIVLNDWSWYEDNGITLHAGC
TVREVDRTRRIVHGTDAQGRTIEAPYDRLILATGSNPFILPIPGNQLSGVLAYRDIADTQAMIDAAAIYRDAVVIGGGLL
GLEAANGLMKRGMRVTVVHASEWLMERQLDDVAGRMLQKSLAERGMGFLMKAQTRELVGDASGRVAAVRFQDGTEVPAQL
VVMAVGIRPSTQLAESMRLHVNRGIVVSDTLQTVTDARIYAVGECAAHRGIAYGLVAPLFEQGKVLANHLAEYGIARYQG
SLTSTKLKVTGIDLFSAGNFQGGEGTEEIVMSDPFGGVYKKLVIRDDQLVGACLYGDTVDGSWYFKLLREGRSIGDIRDK
LMFGESHLGDTGHQGQSKAAAMQDTDEVCGCNGVTKGAICKAIKDKGLFTLEDVRKHTKASASCGSCTGLVEQIIMFTAG
GDYSATPRTKAVCGCTDHGHQAVRDAIRTHRLLTIQDVFKFLEWKTPDGCPTCRPAVNYYLISTWPKEARDDPQSRAINE
RSHANIQKDGTYSVVPRMWGGETTADELRRIADAVDKYNIPTVKVTGGQRIDLLGVKKEDLVNVWKDIGMPSGHAYAKAL
RTVKTCVGSEWCRMGTQDSTQMGKDLERAMWRMYAPHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIAGNGGIKTE
VAHFFTKLKTAEEVLEYTGAFCELYRQEGWYLERTVHYVNRVGLDYVKKRILEDHAGRKALWAQLQFALDGEPDPWFEFD
KAAVDTRQFVPLAPSAAAAEATR
>Mature_823_residues
MKKSRLVMVGNGMAGVRTLEELLKIAPDLYEITVFGAEPHPNYNRILLSPVLAGEQTLEEIVLNDWSWYEDNGITLHAGC
TVREVDRTRRIVHGTDAQGRTIEAPYDRLILATGSNPFILPIPGNQLSGVLAYRDIADTQAMIDAAAIYRDAVVIGGGLL
GLEAANGLMKRGMRVTVVHASEWLMERQLDDVAGRMLQKSLAERGMGFLMKAQTRELVGDASGRVAAVRFQDGTEVPAQL
VVMAVGIRPSTQLAESMRLHVNRGIVVSDTLQTVTDARIYAVGECAAHRGIAYGLVAPLFEQGKVLANHLAEYGIARYQG
SLTSTKLKVTGIDLFSAGNFQGGEGTEEIVMSDPFGGVYKKLVIRDDQLVGACLYGDTVDGSWYFKLLREGRSIGDIRDK
LMFGESHLGDTGHQGQSKAAAMQDTDEVCGCNGVTKGAICKAIKDKGLFTLEDVRKHTKASASCGSCTGLVEQIIMFTAG
GDYSATPRTKAVCGCTDHGHQAVRDAIRTHRLLTIQDVFKFLEWKTPDGCPTCRPAVNYYLISTWPKEARDDPQSRAINE
RSHANIQKDGTYSVVPRMWGGETTADELRRIADAVDKYNIPTVKVTGGQRIDLLGVKKEDLVNVWKDIGMPSGHAYAKAL
RTVKTCVGSEWCRMGTQDSTQMGKDLERAMWRMYAPHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIAGNGGIKTE
VAHFFTKLKTAEEVLEYTGAFCELYRQEGWYLERTVHYVNRVGLDYVKKRILEDHAGRKALWAQLQFALDGEPDPWFEFD
KAAVDTRQFVPLAPSAAAAEATR

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=283, Percent_Identity=26.8551236749117, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI226437568, Length=283, Percent_Identity=26.8551236749117, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI21389617, Length=283, Percent_Identity=26.8551236749117, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1789765, Length=798, Percent_Identity=35.5889724310777, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1789065, Length=311, Percent_Identity=31.8327974276527, Blast_Score=114, Evalue=4e-26,
Organism=Escherichia coli, GI1788892, Length=289, Percent_Identity=25.9515570934256, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=403, Percent_Identity=24.8138957816377, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585130, Length=327, Percent_Identity=24.4648318042813, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281359715, Length=327, Percent_Identity=26.2996941896024, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281359713, Length=327, Percent_Identity=26.2996941896024, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24639250, Length=327, Percent_Identity=26.2996941896024, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18543267, Length=327, Percent_Identity=26.2996941896024, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24639252, Length=327, Percent_Identity=26.2996941896024, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24639257, Length=327, Percent_Identity=26.2996941896024, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28573993, Length=227, Percent_Identity=27.7533039647577, Blast_Score=75, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581020, Length=227, Percent_Identity=27.7533039647577, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 90318; Mature: 90318

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00022 EGF_1 ; PS00037 MYB_1 ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSRLVMVGNGMAGVRTLEELLKIAPDLYEITVFGAEPHPNYNRILLSPVLAGEQTLEE
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEHHHCCHHHHHH
IVLNDWSWYEDNGITLHAGCTVREVDRTRRIVHGTDAQGRTIEAPYDRLILATGSNPFIL
HHHCCCCEECCCCEEEECCCCHHHHHHHHHHEECCCCCCCEEECCCCEEEEEECCCCEEE
PIPGNQLSGVLAYRDIADTQAMIDAAAIYRDAVVIGGGLLGLEAANGLMKRGMRVTVVHA
ECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCEEEEEEH
SEWLMERQLDDVAGRMLQKSLAERGMGFLMKAQTRELVGDASGRVAAVRFQDGTEVPAQL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCEE
VVMAVGIRPSTQLAESMRLHVNRGIVVSDTLQTVTDARIYAVGECAAHRGIAYGLVAPLF
EHEEECCCCHHHHHHHHHHHHCCCEEEECHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHH
EQGKVLANHLAEYGIARYQGSLTSTKLKVTGIDLFSAGNFQGGEGTEEIVMSDPFGGVYK
HCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECCCCHHHH
KLVIRDDQLVGACLYGDTVDGSWYFKLLREGRSIGDIRDKLMFGESHLGDTGHQGQSKAA
HHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHH
AMQDTDEVCGCNGVTKGAICKAIKDKGLFTLEDVRKHTKASASCGSCTGLVEQIIMFTAG
HHCCCHHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHEECC
GDYSATPRTKAVCGCTDHGHQAVRDAIRTHRLLTIQDVFKFLEWKTPDGCPTCRPAVNYY
CCCCCCCCCCEEECCCCCHHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEE
LISTWPKEARDDPQSRAINERSHANIQKDGTYSVVPRMWGGETTADELRRIADAVDKYNI
EEECCCHHHCCCHHHHHCCCHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCC
PTVKVTGGQRIDLLGVKKEDLVNVWKDIGMPSGHAYAKALRTVKTCVGSEWCRMGTQDST
CEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCHH
QMGKDLERAMWRMYAPHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIAGNGGIKTE
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEEEEECCCCCHHH
VAHFFTKLKTAEEVLEYTGAFCELYRQEGWYLERTVHYVNRVGLDYVKKRILEDHAGRKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHH
LWAQLQFALDGEPDPWFEFDKAAVDTRQFVPLAPSAAAAEATR
HHHHEEEEECCCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKSRLVMVGNGMAGVRTLEELLKIAPDLYEITVFGAEPHPNYNRILLSPVLAGEQTLEE
CCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEEHHHCCHHHHHH
IVLNDWSWYEDNGITLHAGCTVREVDRTRRIVHGTDAQGRTIEAPYDRLILATGSNPFIL
HHHCCCCEECCCCEEEECCCCHHHHHHHHHHEECCCCCCCEEECCCCEEEEEECCCCEEE
PIPGNQLSGVLAYRDIADTQAMIDAAAIYRDAVVIGGGLLGLEAANGLMKRGMRVTVVHA
ECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCEEEEEEH
SEWLMERQLDDVAGRMLQKSLAERGMGFLMKAQTRELVGDASGRVAAVRFQDGTEVPAQL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCEE
VVMAVGIRPSTQLAESMRLHVNRGIVVSDTLQTVTDARIYAVGECAAHRGIAYGLVAPLF
EHEEECCCCHHHHHHHHHHHHCCCEEEECHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHH
EQGKVLANHLAEYGIARYQGSLTSTKLKVTGIDLFSAGNFQGGEGTEEIVMSDPFGGVYK
HCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECCCCHHHH
KLVIRDDQLVGACLYGDTVDGSWYFKLLREGRSIGDIRDKLMFGESHLGDTGHQGQSKAA
HHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHH
AMQDTDEVCGCNGVTKGAICKAIKDKGLFTLEDVRKHTKASASCGSCTGLVEQIIMFTAG
HHCCCHHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHEECC
GDYSATPRTKAVCGCTDHGHQAVRDAIRTHRLLTIQDVFKFLEWKTPDGCPTCRPAVNYY
CCCCCCCCCCEEECCCCCHHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCCCCCCCCCEE
LISTWPKEARDDPQSRAINERSHANIQKDGTYSVVPRMWGGETTADELRRIADAVDKYNI
EEECCCHHHCCCHHHHHCCCHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCC
PTVKVTGGQRIDLLGVKKEDLVNVWKDIGMPSGHAYAKALRTVKTCVGSEWCRMGTQDST
CEEEECCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCHH
QMGKDLERAMWRMYAPHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIAGNGGIKTE
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEEECCCCEEEEECCCCCHHH
VAHFFTKLKTAEEVLEYTGAFCELYRQEGWYLERTVHYVNRVGLDYVKKRILEDHAGRKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCHH
LWAQLQFALDGEPDPWFEFDKAAVDTRQFVPLAPSAAAAEATR
HHHHEEEEECCCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]