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The map label for this gene is pit [H]

Identifier: 120610561

GI number: 120610561

Start: 2039090

End: 2040100

Strand: Direct

Name: pit [H]

Synonym: Aave_1881

Alternate gene names: 120610561

Gene position: 2039090-2040100 (Clockwise)

Preceding gene: 120610560

Following gene: 120610564

Centisome position: 38.09

GC content: 66.27

Gene sequence:

>1011_bases
ATGGAAACCGTACAAACCGCCCTGTGGGTCGTCGCCCTCCTGGTCGCGCTCGCCATCCTGTTCGATTTCATGAACGGGTT
CCACGACGCCGCCAATTCGATCGCGACCGTCGTCTCCACCGGCGTTCTCAAGCCCACGCAGGCCGTCATCTTCGCCGCGT
TCTTCAACTTCATCGCGATCTTCATCTTCCACCTGAGCGTGGCCGCCACCATCGGCAAGGGAATCGTGCAGCCCGGGGTG
GTGGACACCCATGTGATCTTCGGCGCGCTGGTGGGTGCCATCACCTGGAACGTCATCACCTGGTACTACGGCATCCCCAG
CAGCTCCTCGCACGCGCTGATCGGCGGCATCGTGGGGGCGGTGATCGCCAAGGCCGGCATCGGGTCGCTGATTTCCGGAG
GCATCTTCAAGACCGTGGCGTTCATCTTCGTGTCGCCGCTGCTCGGCTTCCTGCTCGGCTCCCTCATGATGGTGGCCGTG
GCGTGGATCTTCCGCCGCTCCCGGCCCAGCAAGGTGGACAAGTGGTTCCGCCGCCTGCAGCTGCTCTCCGCCGGCGCCTA
CAGCCTCGGCCACGGCGGCAACGACGCGCAGAAGACGATCGGCATCATCTGGCTGCTGCTGATCGCCACCGGGCATGCCT
CGGCCTCGGACACCGCGCCCCCGACCTGGACCATCGTCGCGTGCTACCTGGCCATCGGCCTGGGCACCATGTTCGGCGGC
TGGCGCATCGTGAAGACGATGGGCCAGAAGATCACCAAGCTCAAGCCGGTCGGCGGCTTCTGTGCCGAGACGGGCGGGGC
GATGACCCTGTTCCTCGCCACCACCCTCGGCATCCCGGTGTCCACCACCCACACCATCACCGGCGCCATCGTCGGCGTGG
GCTCCACCCAGCGCGCAAGCGCCGTGCGCTGGGGCGTGGCCGGCAACATCGTCTGGGCGTGGATCCTCACGATTCCCGCC
AGCGCCTTCGTGGCCGCGATTGCCTACTGGATCAGCCTGCAGCTGTATTGA

Upstream 100 bases:

>100_bases
GCTGGAAACCATCACCGACAAGTGCGAGGACGTGGCCAACCACATCGAGGGCATCATCCTCGAGAACTCCTGATCCGCGG
CGGACGCAAGCGCTCAAAGC

Downstream 100 bases:

>100_bases
TCCGGGCGCCGCGGCGCCGCCTCACTGCGAAATCTTGCGTGCCTCTTCGATCTGGTATTCGAAATAGCGCTGGAAGCTGG
ACGCGATGCTCGCCATGAGC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
METVQTALWVVALLVALAILFDFMNGFHDAANSIATVVSTGVLKPTQAVIFAAFFNFIAIFIFHLSVAATIGKGIVQPGV
VDTHVIFGALVGAITWNVITWYYGIPSSSSHALIGGIVGAVIAKAGIGSLISGGIFKTVAFIFVSPLLGFLLGSLMMVAV
AWIFRRSRPSKVDKWFRRLQLLSAGAYSLGHGGNDAQKTIGIIWLLLIATGHASASDTAPPTWTIVACYLAIGLGTMFGG
WRIVKTMGQKITKLKPVGGFCAETGGAMTLFLATTLGIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWILTIPA
SAFVAAIAYWISLQLY

Sequences:

>Translated_336_residues
METVQTALWVVALLVALAILFDFMNGFHDAANSIATVVSTGVLKPTQAVIFAAFFNFIAIFIFHLSVAATIGKGIVQPGV
VDTHVIFGALVGAITWNVITWYYGIPSSSSHALIGGIVGAVIAKAGIGSLISGGIFKTVAFIFVSPLLGFLLGSLMMVAV
AWIFRRSRPSKVDKWFRRLQLLSAGAYSLGHGGNDAQKTIGIIWLLLIATGHASASDTAPPTWTIVACYLAIGLGTMFGG
WRIVKTMGQKITKLKPVGGFCAETGGAMTLFLATTLGIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWILTIPA
SAFVAAIAYWISLQLY
>Mature_336_residues
METVQTALWVVALLVALAILFDFMNGFHDAANSIATVVSTGVLKPTQAVIFAAFFNFIAIFIFHLSVAATIGKGIVQPGV
VDTHVIFGALVGAITWNVITWYYGIPSSSSHALIGGIVGAVIAKAGIGSLISGGIFKTVAFIFVSPLLGFLLGSLMMVAV
AWIFRRSRPSKVDKWFRRLQLLSAGAYSLGHGGNDAQKTIGIIWLLLIATGHASASDTAPPTWTIVACYLAIGLGTMFGG
WRIVKTMGQKITKLKPVGGFCAETGGAMTLFLATTLGIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWILTIPA
SAFVAAIAYWISLQLY

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=164, Percent_Identity=34.7560975609756, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=36.1842105263158, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=33.1797235023041, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=34.1013824884793, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17539280, Length=170, Percent_Identity=32.9411764705882, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=30.2469135802469, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32566716, Length=174, Percent_Identity=29.8850574712644, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17536725, Length=178, Percent_Identity=29.2134831460674, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557328, Length=155, Percent_Identity=34.8387096774194, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319773, Length=169, Percent_Identity=29.585798816568, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356511, Length=182, Percent_Identity=30.2197802197802, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35345; Mature: 35345

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVQTALWVVALLVALAILFDFMNGFHDAANSIATVVSTGVLKPTQAVIFAAFFNFIAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FIFHLSVAATIGKGIVQPGVVDTHVIFGALVGAITWNVITWYYGIPSSSSHALIGGIVGA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
VIAKAGIGSLISGGIFKTVAFIFVSPLLGFLLGSLMMVAVAWIFRRSRPSKVDKWFRRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLSAGAYSLGHGGNDAQKTIGIIWLLLIATGHASASDTAPPTWTIVACYLAIGLGTMFGG
HHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WRIVKTMGQKITKLKPVGGFCAETGGAMTLFLATTLGIPVSTTHTITGAIVGVGSTQRAS
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCCCHHH
AVRWGVAGNIVWAWILTIPASAFVAAIAYWISLQLY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METVQTALWVVALLVALAILFDFMNGFHDAANSIATVVSTGVLKPTQAVIFAAFFNFIAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FIFHLSVAATIGKGIVQPGVVDTHVIFGALVGAITWNVITWYYGIPSSSSHALIGGIVGA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHH
VIAKAGIGSLISGGIFKTVAFIFVSPLLGFLLGSLMMVAVAWIFRRSRPSKVDKWFRRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLSAGAYSLGHGGNDAQKTIGIIWLLLIATGHASASDTAPPTWTIVACYLAIGLGTMFGG
HHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WRIVKTMGQKITKLKPVGGFCAETGGAMTLFLATTLGIPVSTTHTITGAIVGVGSTQRAS
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCCCHHH
AVRWGVAGNIVWAWILTIPASAFVAAIAYWISLQLY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]