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The map label for this gene is gltX

Identifier: 120610545

GI number: 120610545

Start: 2020280

End: 2021716

Strand: Direct

Name: gltX

Synonym: Aave_1865

Alternate gene names: 120610545

Gene position: 2020280-2021716 (Clockwise)

Preceding gene: 120610544

Following gene: 120610546

Centisome position: 37.74

GC content: 68.06

Gene sequence:

>1437_bases
ATGAGCCAAAGCCTTTCTTCCGCTTCCTCCAGCGGCGCGCCGCAGCGCGTGCGCACCCGTTTCGCGCCGTCCCCCACGGG
CTTCATCCACCTGGGCAACATCCGCTCGGCGCTCTACCCGTGGGCGTTCGCGCGCGCCACGGGCGGCGACTTCATCCTGC
GCATCGAGGACACCGACGTGGAGCGTTCGAGCCAGGCCGCCGTGGATGTCATCCTGGAAGGCATGCAGTGGCTGGGCATG
GAGCCGGACGAAGGCCCGTTCTACCAGATGCAGCGCATGGACCGCTACAAGGAAGTGCTGGCGCAGATGCAGGCGCAGGG
CCTGGTCTATCCGTGCTACATGAGCGTGGCGGAGCTCGACGCGCTGCGCGAGCGCCAGATGCAGGCCAAGCAGAAGCCGC
GCTACGACGGCACGTGGCGCCCGGAGGAGGGCAAGACGCTGCCGCCGGTGCCCGAGGGCGTGCAGCCGGTGCTGCGCTTC
CGCAATCCCGTCGGCGGCTCCGTGGTCTGGGAAGACAAGGTCAAGGGCCGCATCGAGATCCAGAACGACGAGCTCGACGA
CCTCGTCATCGCGCGTCCGGACGGCACGCCCACGTACAACTTCTGCGTGGTGGTGGACGACATCGACATGGCCATCACCC
ACGTGATCCGCGGTGACGACCACGTCAACAACACGCCGCGCCAGATCAACATCTTCCGCGCGCTCGGCAAGGAGCCGCCG
GTGTATGCCCACCTGCCCACCGTGCTCAACGAGCAGGGCGAGAAGATGAGCAAGCGCAACGGCGCCAAGCCCGTCACGCA
GTACCGCGACGAAGGCTACCTGCCCGAGGCGATGGTGAACTACCTCGCGCGCCTGGGCTGGAGCCATGGCGACGACGAAA
TCTTCTCGCGCGAGCAGTTCCTGGAATGGTTCGACCTGGACCACCTGGGCCGCAGCGCGGCGCAGTTCGACGAGGCCAAG
CTGCGCTGGGTGAACGCCCAGCACCTGAAGGCCACGGCCGACGAGCGTCTGGCCGAGCTGGTGGCGCCGCGCCTCGCGGC
CCGCGGCATCGCGCAGTCCGAACTGGCTGACGGCCGGCTGCCACGCATCTGCGCGCTCTTCAAGGACCGCTGCGACACGC
TGGTGGCGCTGGCCGACTGGGCCCATGTGTTCTACGGCGAAGTGACCCCGAACGAGGAGGAACGCGCCAAGCACGTGGTG
GACGCGGTGAAGCCGGCGATCGCCGCGCTCTCGGATGCGCTCGCGCAGTGCGCCTGGGACAAGGCCAGCATCGCCGCCGC
CTTCAAGGAAGTGCTGGCCGCGCAGGGCCTCAAGATGCCCCAATTGGCCATGCCGGTGCGCGTGCTCACCGTGGGCACGG
CGCACACGCCGTCGGTCGATGCGGTGCTCGAACTGCTCGGACGCGAAAAAATTGCAGCGAGATTGCGAACGGCCTGA

Upstream 100 bases:

>100_bases
AGGCCGCCGGCATCGGCCAGGGGCTGATCCGCGTGGCCGTCGGCCTGGAACACATCGAAGACCTGAAGGCCGACCTCGCG
CGCGGCCTGGATACCCTGCA

Downstream 100 bases:

>100_bases
AAACCTGTCTATAATTCAAGGCTGAGGAGATGACGACGATAAATAACAATATCGACGGCATCGAAGTGGGGGTATAGCTC
AGCTGGGAGAGCGCTTGCAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSQSLSSASSSGAPQRVRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVILEGMQWLGM
EPDEGPFYQMQRMDRYKEVLAQMQAQGLVYPCYMSVAELDALRERQMQAKQKPRYDGTWRPEEGKTLPPVPEGVQPVLRF
RNPVGGSVVWEDKVKGRIEIQNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKEPP
VYAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGYLPEAMVNYLARLGWSHGDDEIFSREQFLEWFDLDHLGRSAAQFDEAK
LRWVNAQHLKATADERLAELVAPRLAARGIAQSELADGRLPRICALFKDRCDTLVALADWAHVFYGEVTPNEEERAKHVV
DAVKPAIAALSDALAQCAWDKASIAAAFKEVLAAQGLKMPQLAMPVRVLTVGTAHTPSVDAVLELLGREKIAARLRTA

Sequences:

>Translated_478_residues
MSQSLSSASSSGAPQRVRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVILEGMQWLGM
EPDEGPFYQMQRMDRYKEVLAQMQAQGLVYPCYMSVAELDALRERQMQAKQKPRYDGTWRPEEGKTLPPVPEGVQPVLRF
RNPVGGSVVWEDKVKGRIEIQNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKEPP
VYAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGYLPEAMVNYLARLGWSHGDDEIFSREQFLEWFDLDHLGRSAAQFDEAK
LRWVNAQHLKATADERLAELVAPRLAARGIAQSELADGRLPRICALFKDRCDTLVALADWAHVFYGEVTPNEEERAKHVV
DAVKPAIAALSDALAQCAWDKASIAAAFKEVLAAQGLKMPQLAMPVRVLTVGTAHTPSVDAVLELLGREKIAARLRTA
>Mature_477_residues
SQSLSSASSSGAPQRVRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDVERSSQAAVDVILEGMQWLGME
PDEGPFYQMQRMDRYKEVLAQMQAQGLVYPCYMSVAELDALRERQMQAKQKPRYDGTWRPEEGKTLPPVPEGVQPVLRFR
NPVGGSVVWEDKVKGRIEIQNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKEPPV
YAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGYLPEAMVNYLARLGWSHGDDEIFSREQFLEWFDLDHLGRSAAQFDEAKL
RWVNAQHLKATADERLAELVAPRLAARGIAQSELADGRLPRICALFKDRCDTLVALADWAHVFYGEVTPNEEERAKHVVD
AVKPAIAALSDALAQCAWDKASIAAAFKEVLAAQGLKMPQLAMPVRVLTVGTAHTPSVDAVLELLGREKIAARLRTA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=328, Percent_Identity=35.6707317073171, Blast_Score=206, Evalue=5e-53,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=44.8497854077253, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=34.7368421052632, Blast_Score=142, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=27.4633123689727, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=331, Percent_Identity=36.5558912386707, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321192, Length=215, Percent_Identity=27.4418604651163, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665321, Length=502, Percent_Identity=29.0836653386454, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_ACIAC (A1TNB1)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970223.1
- ProteinModelPortal:   A1TNB1
- SMR:   A1TNB1
- STRING:   A1TNB1
- GeneID:   4666363
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1865
- NMPDR:   fig|397945.5.peg.1615
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NGSGIRD
- PhylomeDB:   A1TNB1
- ProtClustDB:   PRK01406
- BioCyc:   AAVE397945:AAVE_1865-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53282; Mature: 53151

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSLSSASSSGAPQRVRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDV
CCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCC
ERSSQAAVDVILEGMQWLGMEPDEGPFYQMQRMDRYKEVLAQMQAQGLVYPCYMSVAELD
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALRERQMQAKQKPRYDGTWRPEEGKTLPPVPEGVQPVLRFRNPVGGSVVWEDKVKGRIEI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCEEEE
QNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKEPP
CCCCCCCEEEECCCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
VYAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGYLPEAMVNYLARLGWSHGDDEIFSREQF
CEEECHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
LEWFDLDHLGRSAAQFDEAKLRWVNAQHLKATADERLAELVAPRLAARGIAQSELADGRL
HHHHCHHHHCCHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PRICALFKDRCDTLVALADWAHVFYGEVTPNEEERAKHVVDAVKPAIAALSDALAQCAWD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KASIAAAFKEVLAAQGLKMPQLAMPVRVLTVGTAHTPSVDAVLELLGREKIAARLRTA
HHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQSLSSASSSGAPQRVRTRFAPSPTGFIHLGNIRSALYPWAFARATGGDFILRIEDTDV
CCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCC
ERSSQAAVDVILEGMQWLGMEPDEGPFYQMQRMDRYKEVLAQMQAQGLVYPCYMSVAELD
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALRERQMQAKQKPRYDGTWRPEEGKTLPPVPEGVQPVLRFRNPVGGSVVWEDKVKGRIEI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCEEEE
QNDELDDLVIARPDGTPTYNFCVVVDDIDMAITHVIRGDDHVNNTPRQINIFRALGKEPP
CCCCCCCEEEECCCCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
VYAHLPTVLNEQGEKMSKRNGAKPVTQYRDEGYLPEAMVNYLARLGWSHGDDEIFSREQF
CEEECHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
LEWFDLDHLGRSAAQFDEAKLRWVNAQHLKATADERLAELVAPRLAARGIAQSELADGRL
HHHHCHHHHCCHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
PRICALFKDRCDTLVALADWAHVFYGEVTPNEEERAKHVVDAVKPAIAALSDALAQCAWD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KASIAAAFKEVLAAQGLKMPQLAMPVRVLTVGTAHTPSVDAVLELLGREKIAARLRTA
HHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA