Click here to switch to the map view.

The map label for this gene is metZ [H]

Identifier: 120610544

GI number: 120610544

Start: 2019048

End: 2020283

Strand: Direct

Name: metZ [H]

Synonym: Aave_1864

Alternate gene names: 120610544

Gene position: 2019048-2020283 (Clockwise)

Preceding gene: 120610543

Following gene: 120610545

Centisome position: 37.72

GC content: 70.71

Gene sequence:

>1236_bases
GTGGCCCATGACAGAACGAACGGCGCCGGGGGGCTTCCCGACGGCCTGCATCCCGACACGCTCGCCGTGCGGGAATCCAT
TCCGCGCAGCCAGTACGGGGAGCATTCCGAAGCGCTCTACCTGAGCAGCAGCTTCGTGCAGCCCGATGCGCAGACCGCCG
CGCTGCGCTTCGCGGGCGAGGAAGACGGCTACACCTATACGCGCACGGCCAATCCCACGGTGACGGCCTTCGAGCGGCGG
CTGGCCGCCATGGAGGGCACCGAGACCGCCATCGGCACGGCCACCGGCATGTCCGCCATCCTGCTCATGTGCCTGGCGCT
GCTCAAGGCGGGCGACCACGTGGTGTGCTCGCAGTCGATGTTCGGCTCCACCATCAAGCTGATCGGCGGCGAGCTGTCGC
GCTTCGGCGTCGAGACCACCTTCGTGCCGCAGACGGATGCCGCCGCCTGGAAGGCCGCGCTGCGCCCCGCGACGCGCCTG
ATGTTCGCCGAGACGCCCACCAACCCGCTCACCGACCTGTGCGACATCGCCGCGCTGTCGGCCATCGCGCGCGAGGCCGG
CGTGCTGCTGGCCGTGGACAACTGCTTCGCTTCGCCCGCCCTGCAGCAGCCGGCGAAGTGGGGCGCCGACCTCGTCATCC
ATTCCGGCACCAAGTTCCTCGACGGCCAGGGCCGCGTCATGGCCGGCGCCGTGTGCGGCCCGCGCTCGCTCATCGACGAC
AAGCTGGGCCCCATGATCCGCAGCGCGGGCATGAACCTGTCGCCCTTCAACGCCTGGGTGGTGCTCAAGGGGCTGGAGAC
GCTGTCCATCCGCGTGCGGGCCCAGGCCGACAATGCCCTGCGCCTGGCCACGTGGCTTCAGGCGCATCCCGGCGTGGCGC
GCGTCTATTACCCGGGCCTCGCCTCGCATCCCCAGCATGCGCTGGCGATGCGCCAGCAATCCGGCCTGGGCGGCGCGGTG
CTCTCCTTCGACGTGAAGTCGCAGGGCGATGCCGATGCGGCGCGCCGCAGCGCCTTCCACGTCATCGACAGCACGCGCGT
GTGCAGCATCACCGCCAACCTCGGCGACGTGAAGACCACCGTCACCCATCCCGCGAGCACCTCGCACGGGCGCCTGACCG
AGGCGCAGCGCCAGGCCGCCGGCATCGGCCAGGGGCTGATCCGCGTGGCCGTCGGCCTGGAACACATCGAAGACCTGAAG
GCCGACCTCGCGCGCGGCCTGGATACCCTGCAATGA

Upstream 100 bases:

>100_bases
ATCGCGCGCCTGAACGAGGGCCGCGTCGGCAAGGAGGAACAGGAAGAGGACACCTCCCGCCTGGCCCTGCCGAACGCCCA
GGACGCCTGAAGGAAACGCC

Downstream 100 bases:

>100_bases
GCCAAAGCCTTTCTTCCGCTTCCTCCAGCGGCGCGCCGCAGCGCGTGCGCACCCGTTTCGCGCCGTCCCCCACGGGCTTC
ATCCACCTGGGCAACATCCG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MAHDRTNGAGGLPDGLHPDTLAVRESIPRSQYGEHSEALYLSSSFVQPDAQTAALRFAGEEDGYTYTRTANPTVTAFERR
LAAMEGTETAIGTATGMSAILLMCLALLKAGDHVVCSQSMFGSTIKLIGGELSRFGVETTFVPQTDAAAWKAALRPATRL
MFAETPTNPLTDLCDIAALSAIAREAGVLLAVDNCFASPALQQPAKWGADLVIHSGTKFLDGQGRVMAGAVCGPRSLIDD
KLGPMIRSAGMNLSPFNAWVVLKGLETLSIRVRAQADNALRLATWLQAHPGVARVYYPGLASHPQHALAMRQQSGLGGAV
LSFDVKSQGDADAARRSAFHVIDSTRVCSITANLGDVKTTVTHPASTSHGRLTEAQRQAAGIGQGLIRVAVGLEHIEDLK
ADLARGLDTLQ

Sequences:

>Translated_411_residues
MAHDRTNGAGGLPDGLHPDTLAVRESIPRSQYGEHSEALYLSSSFVQPDAQTAALRFAGEEDGYTYTRTANPTVTAFERR
LAAMEGTETAIGTATGMSAILLMCLALLKAGDHVVCSQSMFGSTIKLIGGELSRFGVETTFVPQTDAAAWKAALRPATRL
MFAETPTNPLTDLCDIAALSAIAREAGVLLAVDNCFASPALQQPAKWGADLVIHSGTKFLDGQGRVMAGAVCGPRSLIDD
KLGPMIRSAGMNLSPFNAWVVLKGLETLSIRVRAQADNALRLATWLQAHPGVARVYYPGLASHPQHALAMRQQSGLGGAV
LSFDVKSQGDADAARRSAFHVIDSTRVCSITANLGDVKTTVTHPASTSHGRLTEAQRQAAGIGQGLIRVAVGLEHIEDLK
ADLARGLDTLQ
>Mature_410_residues
AHDRTNGAGGLPDGLHPDTLAVRESIPRSQYGEHSEALYLSSSFVQPDAQTAALRFAGEEDGYTYTRTANPTVTAFERRL
AAMEGTETAIGTATGMSAILLMCLALLKAGDHVVCSQSMFGSTIKLIGGELSRFGVETTFVPQTDAAAWKAALRPATRLM
FAETPTNPLTDLCDIAALSAIAREAGVLLAVDNCFASPALQQPAKWGADLVIHSGTKFLDGQGRVMAGAVCGPRSLIDDK
LGPMIRSAGMNLSPFNAWVVLKGLETLSIRVRAQADNALRLATWLQAHPGVARVYYPGLASHPQHALAMRQQSGLGGAVL
SFDVKSQGDADAARRSAFHVIDSTRVCSITANLGDVKTTVTHPASTSHGRLTEAQRQAAGIGQGLIRVAVGLEHIEDLKA
DLARGLDTLQ

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=335, Percent_Identity=32.5373134328358, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI299473759, Length=335, Percent_Identity=29.5522388059701, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI25453487, Length=334, Percent_Identity=26.9461077844311, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1790375, Length=391, Percent_Identity=36.0613810741688, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1789383, Length=347, Percent_Identity=26.5129682997118, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560056, Length=339, Percent_Identity=34.2182890855457, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17538055, Length=339, Percent_Identity=35.1032448377581, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI71980708, Length=411, Percent_Identity=31.8734793187348, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=32.1951219512195, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=357, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=413, Percent_Identity=26.634382566586, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=302, Percent_Identity=27.8145695364238, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=360, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322970, Length=259, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922554, Length=352, Percent_Identity=33.5227272727273, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43407; Mature: 43276

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDRTNGAGGLPDGLHPDTLAVRESIPRSQYGEHSEALYLSSSFVQPDAQTAALRFAGE
CCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCEEEEEECCC
EDGYTYTRTANPTVTAFERRLAAMEGTETAIGTATGMSAILLMCLALLKAGDHVVCSQSM
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHH
FGSTIKLIGGELSRFGVETTFVPQTDAAAWKAALRPATRLMFAETPTNPLTDLCDIAALS
HCCHHHHHCCHHHHHCCCEEECCCCCHHHHHHHHCHHHHEEEECCCCCHHHHHHHHHHHH
AIAREAGVLLAVDNCFASPALQQPAKWGADLVIHSGTKFLDGQGRVMAGAVCGPRSLIDD
HHHHHCCEEEEECCHHCCCHHCCHHHCCCCEEEECCCEEECCCCCEEEEECCCCHHHHHH
KLGPMIRSAGMNLSPFNAWVVLKGLETLSIRVRAQADNALRLATWLQAHPGVARVYYPGL
HHHHHHHHCCCCCCCCCEEEEEECCEEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCC
ASHPQHALAMRQQSGLGGAVLSFDVKSQGDADAARRSAFHVIDSTRVCSITANLGDVKTT
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHEEECCCEEEEEECCCCCCEEE
VTHPASTSHGRLTEAQRQAAGIGQGLIRVAVGLEHIEDLKADLARGLDTLQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AHDRTNGAGGLPDGLHPDTLAVRESIPRSQYGEHSEALYLSSSFVQPDAQTAALRFAGE
CCCCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCEEEEEECCC
EDGYTYTRTANPTVTAFERRLAAMEGTETAIGTATGMSAILLMCLALLKAGDHVVCSQSM
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHH
FGSTIKLIGGELSRFGVETTFVPQTDAAAWKAALRPATRLMFAETPTNPLTDLCDIAALS
HCCHHHHHCCHHHHHCCCEEECCCCCHHHHHHHHCHHHHEEEECCCCCHHHHHHHHHHHH
AIAREAGVLLAVDNCFASPALQQPAKWGADLVIHSGTKFLDGQGRVMAGAVCGPRSLIDD
HHHHHCCEEEEECCHHCCCHHCCHHHCCCCEEEECCCEEECCCCCEEEEECCCCHHHHHH
KLGPMIRSAGMNLSPFNAWVVLKGLETLSIRVRAQADNALRLATWLQAHPGVARVYYPGL
HHHHHHHHCCCCCCCCCEEEEEECCEEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCC
ASHPQHALAMRQQSGLGGAVLSFDVKSQGDADAARRSAFHVIDSTRVCSITANLGDVKTT
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHEEECCCEEEEEECCCCCCEEE
VTHPASTSHGRLTEAQRQAAGIGQGLIRVAVGLEHIEDLKADLARGLDTLQ
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]