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The map label for this gene is purF [H]

Identifier: 120610543

GI number: 120610543

Start: 2017532

End: 2019037

Strand: Direct

Name: purF [H]

Synonym: Aave_1863

Alternate gene names: 120610543

Gene position: 2017532-2019037 (Clockwise)

Preceding gene: 120610542

Following gene: 120610544

Centisome position: 37.69

GC content: 67.99

Gene sequence:

>1506_bases
ATGTGTGGAATCGTCGGTGTGGTCAGCACGGCTCCCGTCAACCAGCTGATCTATGACGCCTTGCTGCTGCTGCAGCACCG
GGGGCAGGATGCCGCGGGCATCGTCACGCAGCAGGAACGCAAGTTCTTCATGCACAAGGCCAAGGGCATGGTCCGCGACG
TGTTCCGCACCCGCAACATGCGCGCACTGCCCGGCACGGTGGGCCTGGGGCAGGTGCGCTATCCCACGGCCGGCAACGCC
TACAGCGAGGAAGAGGCCCAGCCCTTCTACGTGAACGCCCCGTTCGGCATCGTGCTGGTGCACAACGGCAACCTCACCAA
TGCCCAGTCGCTGCGGGCCGAGCTGTTCTCCACCGACCACCGCCACACCAACACGGAAAGCGACTCCGAGGTGCTGCTCA
ACGTGTTCGCGCACGAACTCGAGCGCGCCACGCGCGGCGTGCCGCTGCAGGTCGAGGACGTCTTCGCCGCGGTGGCCGCC
GTGCACCGGCGCGTGAAGGGCTCCTATGCCGTGATCGCGCTGATCGCGGGCCATGGCCTGCTGGCCTTCCGCGATCCGCA
CGGCATCCGCCCGCTGTGCATCGGCCGCAGCGCGGACGGCACGGTGATGGTGGGCAGCGAATCGGTGGCGCTGGAAGGCA
CCAACCATGTGTTCGAGCGCGACGTGCAGCCCGGCGAGGCCGTGTTCATCACGCCCGACGGCACCGTGCACGCCCGCCAG
TGCGCGCAGGCGCCGCAGCTCAACCCCTGCATCTTCGAGTTCGTCTATCTCGCGCGGCCCGATTCCGTGCTGGACGGCAT
CTCCGTGTACCAGGCGCGCCTCAACCTGGGCGAGGCCCTGGCCAAGCGCGTGGTGTCCACGGTGCCGCCCAACGAGATCG
ACGTGATCATCCCGATCCCCGAATCCAGCCGCCCGAGCGCCACGCAGCTCGCGCACCTGCTCGGCATTCCGTACCGCGAG
GGCTTCGTGAAGAACCGCTACGTGGGCCGCACCTTCATCATGCCGGGGCAGGGCGTGCGCAAGAAGTCCGTGCGCCAGAA
GCTCAACGTGATCGCCAGCGAATTCAAGGGCCGCAACGTGCTGCTGGTGGACGACTCCATCGTGCGCGGCACGACCTCGC
GCGAGATCGTGCAGATGGCCCGCGACGCCGGCGCGCGCAAGGTGTACCTCGCCAGCGCCGCGCCGCCGGTGCGCTATCCC
AACGTGTACGGCATCGACATGCCCACCAGCAATGAGCTGGTCGCGCATGGCCGCACGGTGGAGGAAGTGCGCCAGGCCAT
CGGCTGCGACGCCCTGATCTACCAGGACGTGAATGCCATGAAGAAGGCCGTGGGCTCGCTCAACGGCGGCATCGAGGGCT
TCGACGCTTCGTGCTTCGACGGCGTGTATGTCACGGGCGACATCACCGCGCAGGACATCGCGCGCCTGAACGAGGGCCGC
GTCGGCAAGGAGGAACAGGAAGAGGACACCTCCCGCCTGGCCCTGCCGAACGCCCAGGACGCCTGA

Upstream 100 bases:

>100_bases
CGGTGTTTGTTTCGTTCGCGGCGGCCATCCCTGCAAAGGACGCCGCGGCGGGTTTTTGGGATATCGCGGGCCAGGCCCGC
ATCTGGATGGAACGCTAACT

Downstream 100 bases:

>100_bases
AGGAAACGCCGTGGCCCATGACAGAACGAACGGCGCCGGGGGGCTTCCCGACGGCCTGCATCCCGACACGCTCGCCGTGC
GGGAATCCATTCCGCGCAGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MCGIVGVVSTAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFMHKAKGMVRDVFRTRNMRALPGTVGLGQVRYPTAGNA
YSEEEAQPFYVNAPFGIVLVHNGNLTNAQSLRAELFSTDHRHTNTESDSEVLLNVFAHELERATRGVPLQVEDVFAAVAA
VHRRVKGSYAVIALIAGHGLLAFRDPHGIRPLCIGRSADGTVMVGSESVALEGTNHVFERDVQPGEAVFITPDGTVHARQ
CAQAPQLNPCIFEFVYLARPDSVLDGISVYQARLNLGEALAKRVVSTVPPNEIDVIIPIPESSRPSATQLAHLLGIPYRE
GFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPVRYP
NVYGIDMPTSNELVAHGRTVEEVRQAIGCDALIYQDVNAMKKAVGSLNGGIEGFDASCFDGVYVTGDITAQDIARLNEGR
VGKEEQEEDTSRLALPNAQDA

Sequences:

>Translated_501_residues
MCGIVGVVSTAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFMHKAKGMVRDVFRTRNMRALPGTVGLGQVRYPTAGNA
YSEEEAQPFYVNAPFGIVLVHNGNLTNAQSLRAELFSTDHRHTNTESDSEVLLNVFAHELERATRGVPLQVEDVFAAVAA
VHRRVKGSYAVIALIAGHGLLAFRDPHGIRPLCIGRSADGTVMVGSESVALEGTNHVFERDVQPGEAVFITPDGTVHARQ
CAQAPQLNPCIFEFVYLARPDSVLDGISVYQARLNLGEALAKRVVSTVPPNEIDVIIPIPESSRPSATQLAHLLGIPYRE
GFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPVRYP
NVYGIDMPTSNELVAHGRTVEEVRQAIGCDALIYQDVNAMKKAVGSLNGGIEGFDASCFDGVYVTGDITAQDIARLNEGR
VGKEEQEEDTSRLALPNAQDA
>Mature_501_residues
MCGIVGVVSTAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFMHKAKGMVRDVFRTRNMRALPGTVGLGQVRYPTAGNA
YSEEEAQPFYVNAPFGIVLVHNGNLTNAQSLRAELFSTDHRHTNTESDSEVLLNVFAHELERATRGVPLQVEDVFAAVAA
VHRRVKGSYAVIALIAGHGLLAFRDPHGIRPLCIGRSADGTVMVGSESVALEGTNHVFERDVQPGEAVFITPDGTVHARQ
CAQAPQLNPCIFEFVYLARPDSVLDGISVYQARLNLGEALAKRVVSTVPPNEIDVIIPIPESSRPSATQLAHLLGIPYRE
GFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPVRYP
NVYGIDMPTSNELVAHGRTVEEVRQAIGCDALIYQDVNAMKKAVGSLNGGIEGFDASCFDGVYVTGDITAQDIARLNEGR
VGKEEQEEDTSRLALPNAQDA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=476, Percent_Identity=35.0840336134454, Blast_Score=258, Evalue=7e-69,
Organism=Escherichia coli, GI1788651, Length=473, Percent_Identity=58.985200845666, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1790167, Length=214, Percent_Identity=30.3738317757009, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=485, Percent_Identity=34.020618556701, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=477, Percent_Identity=50.5241090146751, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24659598, Length=499, Percent_Identity=33.8677354709419, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24659604, Length=479, Percent_Identity=34.6555323590814, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28573187, Length=502, Percent_Identity=33.4661354581673, Blast_Score=251, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54461; Mature: 54461

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVVSTAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFMHKAKGMVRDVFRTRNM
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
RALPGTVGLGQVRYPTAGNAYSEEEAQPFYVNAPFGIVLVHNGNLTNAQSLRAELFSTDH
EECCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHCCC
RHTNTESDSEVLLNVFAHELERATRGVPLQVEDVFAAVAAVHRRVKGSYAVIALIAGHGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCE
LAFRDPHGIRPLCIGRSADGTVMVGSESVALEGTNHVFERDVQPGEAVFITPDGTVHARQ
EEEECCCCCCCEEECCCCCCEEEECCCEEEECCCCHHHHCCCCCCCEEEECCCCCCHHHH
CAQAPQLNPCIFEFVYLARPDSVLDGISVYQARLNLGEALAKRVVSTVPPNEIDVIIPIP
HHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
ESSRPSATQLAHLLGIPYREGFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNV
CCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEE
LLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPVRYPNVYGIDMPTSNELVAHGRTV
EEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
EEVRQAIGCDALIYQDVNAMKKAVGSLNGGIEGFDASCFDGVYVTGDITAQDIARLNEGR
HHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCC
VGKEEQEEDTSRLALPNAQDA
CCCCHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MCGIVGVVSTAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFMHKAKGMVRDVFRTRNM
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCC
RALPGTVGLGQVRYPTAGNAYSEEEAQPFYVNAPFGIVLVHNGNLTNAQSLRAELFSTDH
EECCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHCCC
RHTNTESDSEVLLNVFAHELERATRGVPLQVEDVFAAVAAVHRRVKGSYAVIALIAGHGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCE
LAFRDPHGIRPLCIGRSADGTVMVGSESVALEGTNHVFERDVQPGEAVFITPDGTVHARQ
EEEECCCCCCCEEECCCCCCEEEECCCEEEECCCCHHHHCCCCCCCEEEECCCCCCHHHH
CAQAPQLNPCIFEFVYLARPDSVLDGISVYQARLNLGEALAKRVVSTVPPNEIDVIIPIP
HHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
ESSRPSATQLAHLLGIPYREGFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNV
CCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEE
LLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPVRYPNVYGIDMPTSNELVAHGRTV
EEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
EEVRQAIGCDALIYQDVNAMKKAVGSLNGGIEGFDASCFDGVYVTGDITAQDIARLNEGR
HHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCC
VGKEEQEEDTSRLALPNAQDA
CCCCHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]