Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 120610341

GI number: 120610341

Start: 1818677

End: 1820227

Strand: Direct

Name: gsiB [H]

Synonym: Aave_1658

Alternate gene names: 120610341

Gene position: 1818677-1820227 (Clockwise)

Preceding gene: 120610340

Following gene: 120610342

Centisome position: 33.98

GC content: 64.41

Gene sequence:

>1551_bases
ATGAAGAAGACCCCCGCTTCCCGCCGCGTCGCCGCCAGCCTGCTGGCGCTGGCCGCCCTGTCCGGCACCGGCTCGGCGCT
CGCCGCCGGCAATGCCGTGCTGGCCATCTACCAGCAGCCCGAGACGCTGGACCCCTACAACACGAACACGACCATCACCA
CGGCCGTCACCAAGAGCTTCTACGAAGGCCTGTTCGCGTTCGACAAGGACATGAAGCTCAAGAACGTGCTGGCCGAGAGC
TATGAGGTTTCCAAGGACGGCCTGGTCTATACCTTCAAGCTGCGTTCGGGCGTGAAGTTCCACGACGGCACCGACTTCAA
CGCCGCCGCGGCCAAGGCCAACCTGGACCGCGTGATGGACCCGGCCAACCGCCTGCTGCGCGCCAACCAGTTCAACCGGG
TGGCCAAGGTCGAGGCCGTGAATCCGCAGACCCTGCGCATCACGCTCAAGGAGCCCTTCGGCCCCTTCATCAACGCGCTG
GGCCATGCCTCGGCCGCGATGATCTCGCCCGCGGCGCTCGCCAAGTGGGGCAGCAAGGACATCGCCTTCCACCCCGTGGG
CACCGGCCCGTTCGAGTTCGTCGAGTGGAAGCAGACCGAAGCCATCGTGGGCAAGAAGTTCGCCAACTACTGGAAGAAGG
GCTATCCCAAGGTGGACCAGGTGACCTGGAAGCCCGTCACCGAGAACAACACCCGCGCCGCCATGCTGCAGACGGGCGAG
GCCGACTTCGCCTTCACGCTGCCCTATGAGCAGATCGCCACGCTGAAGAAGAGCGACAAGGTGGAAGTGGTGACCGCGCC
CTCCATCATCCAGCGCTTCCTGACGTTCAACATGCTGCAGAAGCCCTTCGACAACCCGAAGGTGCGCGAGGCCATCGGCT
ACGCCATCAACAAGGAGGCGCTGTCCAAGGTGGCCTTCAACGGCTACGCCTTCCCGGCCCAGGGCTTCGTGCCCCAGGGC
GTGGAATACGCCGTGAAGATGCAGCCGATTCCCTACAACGTGGCCAAGGCCAGGGAACTGCTGAAGGAAGCCGGCTACCC
CAACGGTTTCGAGACCGTGCTGTGGAGCGGCTACAACAACACCACCAGCCAGAAGGTGATCCAGTTCCTGCAGCAGCAGC
TGCAGCAGGTGGGCATCAAGGTGTCGGTGGAGGCGCTGGAGCCCGGCAAGCGCGCCGAAACAGTCGATGCCTGGCCCGAT
CCCAAGACCTCCAAGCTGCGCCTGTACTACATCGGCTGGTCGTCCTCCACCGGCGAGGCCGACTGGGCGCTGCGTCCGCT
GTTCTCCACCGAATCGTGGGCGCCCAAGCTCGCGAACTACGCCTTCTACAGCAACCAGGTGGTGGATGACGCCATCGCCA
AGGCGCTGTTGAGCACCGACAGCTCCGAGCGTGCCGCGCTCTACAAGACCGCGCAGGAGCAGCTCGCCAAGGACCTGCCG
CGCATTCCGCTGGTCACCGAGGAAGTGCTCTACGCCCACGCCAAGCGCCTCTCGGGCGTGTACGTGATGCCCGACGGCAA
CATCAACAGCGAAGAAATCTCGCTGAAGTAA

Upstream 100 bases:

>100_bases
GGTGGGCCCGGGCCATTCCGTGGCGCGCCACGCCATCGCCAACCTGTACTGATCCCGCCTTTCCTTCCTGCCTTCGTTCC
TTCCCCAACCGGAGTCCACC

Downstream 100 bases:

>100_bases
GCCCGGTGCACGGCCCCCGCAGACAGCGGGGGCCCTTTTTCCCGTTTCCCGCTCCGCTCCCGTTTTCCCCCTCGCTGCAC
ACCGCGGCGCCGCATCATGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKKTPASRRVAASLLALAALSGTGSALAAGNAVLAIYQQPETLDPYNTNTTITTAVTKSFYEGLFAFDKDMKLKNVLAES
YEVSKDGLVYTFKLRSGVKFHDGTDFNAAAAKANLDRVMDPANRLLRANQFNRVAKVEAVNPQTLRITLKEPFGPFINAL
GHASAAMISPAALAKWGSKDIAFHPVGTGPFEFVEWKQTEAIVGKKFANYWKKGYPKVDQVTWKPVTENNTRAAMLQTGE
ADFAFTLPYEQIATLKKSDKVEVVTAPSIIQRFLTFNMLQKPFDNPKVREAIGYAINKEALSKVAFNGYAFPAQGFVPQG
VEYAVKMQPIPYNVAKARELLKEAGYPNGFETVLWSGYNNTTSQKVIQFLQQQLQQVGIKVSVEALEPGKRAETVDAWPD
PKTSKLRLYYIGWSSSTGEADWALRPLFSTESWAPKLANYAFYSNQVVDDAIAKALLSTDSSERAALYKTAQEQLAKDLP
RIPLVTEEVLYAHAKRLSGVYVMPDGNINSEEISLK

Sequences:

>Translated_516_residues
MKKTPASRRVAASLLALAALSGTGSALAAGNAVLAIYQQPETLDPYNTNTTITTAVTKSFYEGLFAFDKDMKLKNVLAES
YEVSKDGLVYTFKLRSGVKFHDGTDFNAAAAKANLDRVMDPANRLLRANQFNRVAKVEAVNPQTLRITLKEPFGPFINAL
GHASAAMISPAALAKWGSKDIAFHPVGTGPFEFVEWKQTEAIVGKKFANYWKKGYPKVDQVTWKPVTENNTRAAMLQTGE
ADFAFTLPYEQIATLKKSDKVEVVTAPSIIQRFLTFNMLQKPFDNPKVREAIGYAINKEALSKVAFNGYAFPAQGFVPQG
VEYAVKMQPIPYNVAKARELLKEAGYPNGFETVLWSGYNNTTSQKVIQFLQQQLQQVGIKVSVEALEPGKRAETVDAWPD
PKTSKLRLYYIGWSSSTGEADWALRPLFSTESWAPKLANYAFYSNQVVDDAIAKALLSTDSSERAALYKTAQEQLAKDLP
RIPLVTEEVLYAHAKRLSGVYVMPDGNINSEEISLK
>Mature_516_residues
MKKTPASRRVAASLLALAALSGTGSALAAGNAVLAIYQQPETLDPYNTNTTITTAVTKSFYEGLFAFDKDMKLKNVLAES
YEVSKDGLVYTFKLRSGVKFHDGTDFNAAAAKANLDRVMDPANRLLRANQFNRVAKVEAVNPQTLRITLKEPFGPFINAL
GHASAAMISPAALAKWGSKDIAFHPVGTGPFEFVEWKQTEAIVGKKFANYWKKGYPKVDQVTWKPVTENNTRAAMLQTGE
ADFAFTLPYEQIATLKKSDKVEVVTAPSIIQRFLTFNMLQKPFDNPKVREAIGYAINKEALSKVAFNGYAFPAQGFVPQG
VEYAVKMQPIPYNVAKARELLKEAGYPNGFETVLWSGYNNTTSQKVIQFLQQQLQQVGIKVSVEALEPGKRAETVDAWPD
PKTSKLRLYYIGWSSSTGEADWALRPLFSTESWAPKLANYAFYSNQVVDDAIAKALLSTDSSERAALYKTAQEQLAKDLP
RIPLVTEEVLYAHAKRLSGVYVMPDGNINSEEISLK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=511, Percent_Identity=56.3600782778865, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1789966, Length=537, Percent_Identity=27.56052141527, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787551, Length=541, Percent_Identity=27.9112754158965, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787762, Length=515, Percent_Identity=25.2427184466019, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787495, Length=519, Percent_Identity=26.3969171483622, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789887, Length=495, Percent_Identity=25.0505050505051, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789397, Length=509, Percent_Identity=23.7721021611002, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081878, Length=506, Percent_Identity=21.9367588932806, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56973; Mature: 56973

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTPASRRVAASLLALAALSGTGSALAAGNAVLAIYQQPETLDPYNTNTTITTAVTKSF
CCCCCHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCCCEEHHHHHHHH
YEGLFAFDKDMKLKNVLAESYEVSKDGLVYTFKLRSGVKFHDGTDFNAAAAKANLDRVMD
HHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHC
PANRLLRANQFNRVAKVEAVNPQTLRITLKEPFGPFINALGHASAAMISPAALAKWGSKD
HHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCHHHHHHCCCHHHHCCHHHHHHCCCCC
IAFHPVGTGPFEFVEWKQTEAIVGKKFANYWKKGYPKVDQVTWKPVTENNTRAAMLQTGE
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEECCC
ADFAFTLPYEQIATLKKSDKVEVVTAPSIIQRFLTFNMLQKPFDNPKVREAIGYAINKEA
CCEEEECCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHH
LSKVAFNGYAFPAQGFVPQGVEYAVKMQPIPYNVAKARELLKEAGYPNGFETVLWSGYNN
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCC
TTSQKVIQFLQQQLQQVGIKVSVEALEPGKRAETVDAWPDPKTSKLRLYYIGWSSSTGEA
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
DWALRPLFSTESWAPKLANYAFYSNQVVDDAIAKALLSTDSSERAALYKTAQEQLAKDLP
CCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
RIPLVTEEVLYAHAKRLSGVYVMPDGNINSEEISLK
CCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEECC
>Mature Secondary Structure
MKKTPASRRVAASLLALAALSGTGSALAAGNAVLAIYQQPETLDPYNTNTTITTAVTKSF
CCCCCHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCCCEEHHHHHHHH
YEGLFAFDKDMKLKNVLAESYEVSKDGLVYTFKLRSGVKFHDGTDFNAAAAKANLDRVMD
HHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHC
PANRLLRANQFNRVAKVEAVNPQTLRITLKEPFGPFINALGHASAAMISPAALAKWGSKD
HHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCHHHHHHCCCHHHHCCHHHHHHCCCCC
IAFHPVGTGPFEFVEWKQTEAIVGKKFANYWKKGYPKVDQVTWKPVTENNTRAAMLQTGE
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEECCC
ADFAFTLPYEQIATLKKSDKVEVVTAPSIIQRFLTFNMLQKPFDNPKVREAIGYAINKEA
CCEEEECCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHH
LSKVAFNGYAFPAQGFVPQGVEYAVKMQPIPYNVAKARELLKEAGYPNGFETVLWSGYNN
HHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCC
TTSQKVIQFLQQQLQQVGIKVSVEALEPGKRAETVDAWPDPKTSKLRLYYIGWSSSTGEA
CHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCC
DWALRPLFSTESWAPKLANYAFYSNQVVDDAIAKALLSTDSSERAALYKTAQEQLAKDLP
CCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
RIPLVTEEVLYAHAKRLSGVYVMPDGNINSEEISLK
CCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA