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The map label for this gene is citH [H]

Identifier: 120610329

GI number: 120610329

Start: 1804648

End: 1805967

Strand: Direct

Name: citH [H]

Synonym: Aave_1646

Alternate gene names: 120610329

Gene position: 1804648-1805967 (Clockwise)

Preceding gene: 120610328

Following gene: 120610334

Centisome position: 33.71

GC content: 67.88

Gene sequence:

>1320_bases
ATGACTCCTTCACACCAATCCTTCATCGCGGCTTCCGGCAGCGAAGCCGCCCCGTCCGCGCCCGCGCAGTCCAAGGTGGG
CACCGTCCTGCGCGTGACGGGCGGCAATTTCATGGAGATGTTCGATTTTTTCCTGTTCGGCTTCTACGCCACGCAGATCT
CCAAGGCGTTCTTCCCCGCAGGCAACGAGTTCGCCTCGCTCATGCTGACGTTCATGACCTTCGGCGCGGGCTTCCTCATG
CGGCCGCTGGGGGCGGTGTTCCTCGGGGCGTACGTGGACCGCGTCGGCCGCCGCAAGGGGCTGATCGTGACGCTGGCGCT
GATGGCGCTGGGCACGCTGCTGATCGCCTGCGTGCCCGGCCACGCCACCATCGGCCTGGCGGCGCCGCTGCTGGTGCTGA
TCGGGCGCCTGCTGCAGGGCTTCTCGGCCGGCGTGGAGCTGGGCGGGGTGTCGGTGTACCTTGCCGAGATAGCGACACCG
GGCCGCAAGGGCTTCTACGTGAGCTGGCAGTCGGCCAGCCAGCAGGTGGCGATCATCGTCGCGGCGGCGCTGGGCTACTG
GCTCAACGTGACCTTCGTGCCGCAGGAGATCGCGGACTTCTACTGGCGGGTGCCGTTCTTCGTGGGTTGCCTGATCGTGC
CGGTGCTGTTCATCATCCGCCGCTCGCTGCAGGAGACCGAGGAGTTCAAGGCGCGCAGGCACCGTCCTTCCACGCGCGAG
ATCTTCCGCTCCATCGTCTCCAACTGGGGCTTGGTGATCGCGGGGATGATGATGGTGTCGATGACGACCGTGTCGTTCTA
CCTCATCACCGTGTACACGCCGACCTTCGGCAAGAGCGTGCTGCACCTGAGCACCACGGACGCGCTGGTCGTGACGCTGT
GCGTGGCGCTGTCCAACTTCTTCTGGCTGCCGGTGATGGGCGCGCTCTCCGACCGCGTGGGCCGCAAGCCGCTGCTGGTG
GCCTTCACCGTGCTGACCATCTTCACCGCCTATCCGGCCCTGAAGTGGCTGGTGGCCGCTCCCGATTTCGCGCGCATGCT
GGAGGTGGAGCTGTGGCTGTCCTTCCTGTACGCGAGCTACAACGGCGCGATGGTCGTCGCGCTGACCGAGGTGATGCCGG
TGGACGTGCGCACGGTGGGCTTCTCGCTGGCCTACAGCCTGGCGACCGCGGTGTTCGGCGGCTTCACGCCGGCCGTCGCC
ACGGGCCTGATCGAATGGTCGGGCGACAAGGCCGCTCCGGGCTGGTGGATGACGGCCGCCGCGGTCTGCGGCCTGCTGGC
CACGCTGCGGCTGTACCGCCGCGCGGCCGGCCGCGGCTGA

Upstream 100 bases:

>100_bases
TACGCAATTCCACGGAAGGATTTCGCGAACGGCTGAGCTCTAATTCCACTGTTTCCACTCCTGGACGCGCGTGCCTGTCG
TCGCCGCGCTCCTGCCATCC

Downstream 100 bases:

>100_bases
AGGCCCGCCGTCCGCAAGCCCGGAGCCGGCGGTATCCGGGCTGGGCGTCGCATCAGACTTCCCTTTGGTGGATCGTGATG
TGGGGCAGGCGGGAGGATCG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTPSHQSFIAASGSEAAPSAPAQSKVGTVLRVTGGNFMEMFDFFLFGFYATQISKAFFPAGNEFASLMLTFMTFGAGFLM
RPLGAVFLGAYVDRVGRRKGLIVTLALMALGTLLIACVPGHATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEIATP
GRKGFYVSWQSASQQVAIIVAAALGYWLNVTFVPQEIADFYWRVPFFVGCLIVPVLFIIRRSLQETEEFKARRHRPSTRE
IFRSIVSNWGLVIAGMMMVSMTTVSFYLITVYTPTFGKSVLHLSTTDALVVTLCVALSNFFWLPVMGALSDRVGRKPLLV
AFTVLTIFTAYPALKWLVAAPDFARMLEVELWLSFLYASYNGAMVVALTEVMPVDVRTVGFSLAYSLATAVFGGFTPAVA
TGLIEWSGDKAAPGWWMTAAAVCGLLATLRLYRRAAGRG

Sequences:

>Translated_439_residues
MTPSHQSFIAASGSEAAPSAPAQSKVGTVLRVTGGNFMEMFDFFLFGFYATQISKAFFPAGNEFASLMLTFMTFGAGFLM
RPLGAVFLGAYVDRVGRRKGLIVTLALMALGTLLIACVPGHATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEIATP
GRKGFYVSWQSASQQVAIIVAAALGYWLNVTFVPQEIADFYWRVPFFVGCLIVPVLFIIRRSLQETEEFKARRHRPSTRE
IFRSIVSNWGLVIAGMMMVSMTTVSFYLITVYTPTFGKSVLHLSTTDALVVTLCVALSNFFWLPVMGALSDRVGRKPLLV
AFTVLTIFTAYPALKWLVAAPDFARMLEVELWLSFLYASYNGAMVVALTEVMPVDVRTVGFSLAYSLATAVFGGFTPAVA
TGLIEWSGDKAAPGWWMTAAAVCGLLATLRLYRRAAGRG
>Mature_438_residues
TPSHQSFIAASGSEAAPSAPAQSKVGTVLRVTGGNFMEMFDFFLFGFYATQISKAFFPAGNEFASLMLTFMTFGAGFLMR
PLGAVFLGAYVDRVGRRKGLIVTLALMALGTLLIACVPGHATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEIATPG
RKGFYVSWQSASQQVAIIVAAALGYWLNVTFVPQEIADFYWRVPFFVGCLIVPVLFIIRRSLQETEEFKARRHRPSTREI
FRSIVSNWGLVIAGMMMVSMTTVSFYLITVYTPTFGKSVLHLSTTDALVVTLCVALSNFFWLPVMGALSDRVGRKPLLVA
FTVLTIFTAYPALKWLVAAPDFARMLEVELWLSFLYASYNGAMVVALTEVMPVDVRTVGFSLAYSLATAVFGGFTPAVAT
GLIEWSGDKAAPGWWMTAAAVCGLLATLRLYRRAAGRG

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=29.8578199052133, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=32.4825986078886, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788292, Length=385, Percent_Identity=27.2727272727273, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=27.8846153846154, Blast_Score=124, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47523; Mature: 47391

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSHQSFIAASGSEAAPSAPAQSKVGTVLRVTGGNFMEMFDFFLFGFYATQISKAFFPA
CCCCCCCCEEECCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
GNEFASLMLTFMTFGAGFLMRPLGAVFLGAYVDRVGRRKGLIVTLALMALGTLLIACVPG
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
HATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEIATPGRKGFYVSWQSASQQVAIIV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEHHHHCCCCCCCEEEEECCCCCHHHHHH
AAALGYWLNVTFVPQEIADFYWRVPFFVGCLIVPVLFIIRRSLQETEEFKARRHRPSTRE
HHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IFRSIVSNWGLVIAGMMMVSMTTVSFYLITVYTPTFGKSVLHLSTTDALVVTLCVALSNF
HHHHHHHCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHH
FWLPVMGALSDRVGRKPLLVAFTVLTIFTAYPALKWLVAAPDFARMLEVELWLSFLYASY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
NGAMVVALTEVMPVDVRTVGFSLAYSLATAVFGGFTPAVATGLIEWSGDKAAPGWWMTAA
CCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHHHHH
AVCGLLATLRLYRRAAGRG
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPSHQSFIAASGSEAAPSAPAQSKVGTVLRVTGGNFMEMFDFFLFGFYATQISKAFFPA
CCCCCCCEEECCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
GNEFASLMLTFMTFGAGFLMRPLGAVFLGAYVDRVGRRKGLIVTLALMALGTLLIACVPG
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
HATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYLAEIATPGRKGFYVSWQSASQQVAIIV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEHHHHCCCCCCCEEEEECCCCCHHHHHH
AAALGYWLNVTFVPQEIADFYWRVPFFVGCLIVPVLFIIRRSLQETEEFKARRHRPSTRE
HHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IFRSIVSNWGLVIAGMMMVSMTTVSFYLITVYTPTFGKSVLHLSTTDALVVTLCVALSNF
HHHHHHHCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHH
FWLPVMGALSDRVGRKPLLVAFTVLTIFTAYPALKWLVAAPDFARMLEVELWLSFLYASY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
NGAMVVALTEVMPVDVRTVGFSLAYSLATAVFGGFTPAVATGLIEWSGDKAAPGWWMTAA
CCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHHHHH
AVCGLLATLRLYRRAAGRG
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]