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The map label for this gene is rimO

Identifier: 120610328

GI number: 120610328

Start: 1802834

End: 1804225

Strand: Direct

Name: rimO

Synonym: Aave_1645

Alternate gene names: 120610328

Gene position: 1802834-1804225 (Clockwise)

Preceding gene: 120610324

Following gene: 120610329

Centisome position: 33.68

GC content: 67.67

Gene sequence:

>1392_bases
ATGAACGAAACGATTGCACCGGCCCGCCTGCCGCGTATAGGTATGGTATCCCTCGGGTGCCCGAAGGCCCTCACGGATTC
CGAACTCATCCTCACGCAGCTGAGCGCCGAGGGTTACCAGACTTCCAAGACCTTCGAGGGCGCCGACCTCGTCATCGTCA
ACACCTGCGGCTTCATCGACGATGCCGTGAAGGAAAGCCTCGACACCATCGGCGAGGCGCTGGCCGAGAACGGCAAGGTG
ATCGTCACCGGCTGCCTGGGCGCGCGCGCGGGCGAGGGCGGCGGCAACCTGGTGCGCGAAATGCACCCTAGCGTGCTGGC
CGTGACCGGCCCGCATGCCACGCAGGAGGTGATGGACGCCGTCCACCAGAACCTGCCCAAGCCGCACGATCCGTTCATCG
ACCTCGTGCCCGGCGGCTTCGGCGTGGCGGGCGTGAAGCTCACGCCCAGGCACTATGCGTACCTGAAGATCAGCGAGGGC
TGCAACCACCGCTGCACCTTCTGCATCATCCCCTCGATGCGCGGCGACCTGGTCTCGCGCCCCGTGGGCGATGTGCTGAG
CGAGGCGAAGGCGCTCTTCGAGGGTGGCGTGAAGGAACTGCTGGTGATCAGCCAGGACACGTCCGCCTACGGCGTGGACG
TGAAGTACCGCACCGGCTTCTGGGACGGCAAGCCGGTCAAGACGCGCATGCTGGAGCTGGTGCAGACGCTGGGCGAGATC
GCCGAGCCCTACGGCGCCTGGGTGCGCCTGCATTACGTGTATCCGTACCCCAGCGTGGACGAGGTGATCCCGTTCATGGC
CACGGGCAAGATCCTGCCCTACCTGGACGTGCCGTTCCAGCACAGCCACCCCGACGTGCTCCGGCGCATGAAGCGGCCTG
CGAGCGGCGAGCGCAACCTGGAGCGCATCCAGCGCTGGCGCGAGGCCTGCCCGGAGCTGGTGATCCGCAGCACCTTCATC
GCGGGCTTCCCCGGCGAGACGGAAGAAGAATTCCAGCACCTGCTGGACTTCCTGCGCGAAGCGCAGATCGACCGCGCGGG
CTGCTTCGCCTACAGCGACGTGGACGGCGCCGTCGCGAACGAGCTTCCCGGCATGCTGCCGATGGAAGTGCGCGAGGAGC
GCCGCGCGCGCTTCATGGCCGTGGCCGAAGAGGTTTCCATCGAACGCCTGCGCCGCCGCGTGGGCGCGACCATGCAGGTG
CTGGTGGACCATGCCCCGGCGCTGGGCCGCAAGGGCGGCCGGGGCCGCAGCTATGCCGATGCGCCGGAAATCGACGGCGT
GGTGCACCTGCTGCCGCCCGAGAAGATCAGCAAGCAGCTGAAGGTGGGCGAATTCACGCGCGCGCAGATCGTGGGCGTGC
AGGGGCACGACCTGGTCGCAAGACCGATCTGA

Upstream 100 bases:

>100_bases
CGCGTACGCCTCCTGGCGCTCGCGGCTGTGGCCAGCGGCCTGGCGCGGATCTGAAAACCGCATGTAGCTGTAGACAAGAC
CTTTCAAGACACATTCCCCC

Downstream 100 bases:

>100_bases
ATCCCCCCCTGGGTCGCCTTCGGCGCCTCCGCGCAGCCGCCGGAGTCGGCAGCTCTTCAGGCACGTCCAGGCCTGCGCAG
GCAGGCCCGGAGCCGCGGCC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNETIAPARLPRIGMVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFIDDAVKESLDTIGEALAENGKV
IVTGCLGARAGEGGGNLVREMHPSVLAVTGPHATQEVMDAVHQNLPKPHDPFIDLVPGGFGVAGVKLTPRHYAYLKISEG
CNHRCTFCIIPSMRGDLVSRPVGDVLSEAKALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELVQTLGEI
AEPYGAWVRLHYVYPYPSVDEVIPFMATGKILPYLDVPFQHSHPDVLRRMKRPASGERNLERIQRWREACPELVIRSTFI
AGFPGETEEEFQHLLDFLREAQIDRAGCFAYSDVDGAVANELPGMLPMEVREERRARFMAVAEEVSIERLRRRVGATMQV
LVDHAPALGRKGGRGRSYADAPEIDGVVHLLPPEKISKQLKVGEFTRAQIVGVQGHDLVARPI

Sequences:

>Translated_463_residues
MNETIAPARLPRIGMVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFIDDAVKESLDTIGEALAENGKV
IVTGCLGARAGEGGGNLVREMHPSVLAVTGPHATQEVMDAVHQNLPKPHDPFIDLVPGGFGVAGVKLTPRHYAYLKISEG
CNHRCTFCIIPSMRGDLVSRPVGDVLSEAKALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELVQTLGEI
AEPYGAWVRLHYVYPYPSVDEVIPFMATGKILPYLDVPFQHSHPDVLRRMKRPASGERNLERIQRWREACPELVIRSTFI
AGFPGETEEEFQHLLDFLREAQIDRAGCFAYSDVDGAVANELPGMLPMEVREERRARFMAVAEEVSIERLRRRVGATMQV
LVDHAPALGRKGGRGRSYADAPEIDGVVHLLPPEKISKQLKVGEFTRAQIVGVQGHDLVARPI
>Mature_463_residues
MNETIAPARLPRIGMVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFIDDAVKESLDTIGEALAENGKV
IVTGCLGARAGEGGGNLVREMHPSVLAVTGPHATQEVMDAVHQNLPKPHDPFIDLVPGGFGVAGVKLTPRHYAYLKISEG
CNHRCTFCIIPSMRGDLVSRPVGDVLSEAKALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELVQTLGEI
AEPYGAWVRLHYVYPYPSVDEVIPFMATGKILPYLDVPFQHSHPDVLRRMKRPASGERNLERIQRWREACPELVIRSTFI
AGFPGETEEEFQHLLDFLREAQIDRAGCFAYSDVDGAVANELPGMLPMEVREERRARFMAVAEEVSIERLRRRVGATMQV
LVDHAPALGRKGGRGRSYADAPEIDGVVHLLPPEKISKQLKVGEFTRAQIVGVQGHDLVARPI

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=429, Percent_Identity=26.1072261072261, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28872782, Length=429, Percent_Identity=26.1072261072261, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI93277076, Length=404, Percent_Identity=24.7524752475248, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=61.0244988864143, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1786882, Length=407, Percent_Identity=27.2727272727273, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=487, Percent_Identity=23.2032854209446, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=28.343949044586, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356207, Length=422, Percent_Identity=26.5402843601896, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922432, Length=350, Percent_Identity=27.4285714285714, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_ACIAC (A1TMP4)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970006.1
- ProteinModelPortal:   A1TMP4
- SMR:   A1TMP4
- STRING:   A1TMP4
- GeneID:   4666756
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1645
- NMPDR:   fig|397945.5.peg.1443
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   A1TMP4
- ProtClustDB:   PRK14862
- BioCyc:   AAVE397945:AAVE_1645-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50827; Mature: 50827

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETIAPARLPRIGMVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFID
CCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHH
DAVKESLDTIGEALAENGKVIVTGCLGARAGEGGGNLVREMHPSVLAVTGPHATQEVMDA
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHH
VHQNLPKPHDPFIDLVPGGFGVAGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSR
HHHCCCCCCCCCEEECCCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHC
PVGDVLSEAKALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELVQTLGEI
CHHHHHHHHHHHHHCCCHHEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
AEPYGAWVRLHYVYPYPSVDEVIPFMATGKILPYLDVPFQHSHPDVLRRMKRPASGERNL
HCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCCCCHHH
ERIQRWREACPELVIRSTFIAGFPGETEEEFQHLLDFLREAQIDRAGCFAYSDVDGAVAN
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHCCEEECCCCCHHHH
ELPGMLPMEVREERRARFMAVAEEVSIERLRRRVGATMQVLVDHAPALGRKGGRGRSYAD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCCCCCCCCCC
APEIDGVVHLLPPEKISKQLKVGEFTRAQIVGVQGHDLVARPI
CCCCCCEEEECCCHHHHHHCCCCCCCEEEEEECCCCCEECCCC
>Mature Secondary Structure
MNETIAPARLPRIGMVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFID
CCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHH
DAVKESLDTIGEALAENGKVIVTGCLGARAGEGGGNLVREMHPSVLAVTGPHATQEVMDA
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHH
VHQNLPKPHDPFIDLVPGGFGVAGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSR
HHHCCCCCCCCCEEECCCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHC
PVGDVLSEAKALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELVQTLGEI
CHHHHHHHHHHHHHCCCHHEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
AEPYGAWVRLHYVYPYPSVDEVIPFMATGKILPYLDVPFQHSHPDVLRRMKRPASGERNL
HCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCCCCHHH
ERIQRWREACPELVIRSTFIAGFPGETEEEFQHLLDFLREAQIDRAGCFAYSDVDGAVAN
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHCCEEECCCCCHHHH
ELPGMLPMEVREERRARFMAVAEEVSIERLRRRVGATMQVLVDHAPALGRKGGRGRSYAD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCCCCCCCCCC
APEIDGVVHLLPPEKISKQLKVGEFTRAQIVGVQGHDLVARPI
CCCCCCEEEECCCHHHHHHCCCCCCCEEEEEECCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA