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The map label for this gene is ampD [H]

Identifier: 120609559

GI number: 120609559

Start: 938808

End: 939437

Strand: Reverse

Name: ampD [H]

Synonym: Aave_0865

Alternate gene names: 120609559

Gene position: 939437-938808 (Counterclockwise)

Preceding gene: 120609560

Following gene: 120609558

Centisome position: 17.55

GC content: 71.43

Gene sequence:

>630_bases
ATGCAGCCTCCTGACCCGAACGACGCTCCCGGGCACGGCGGCCCGGATGAAGCCGGCCCGCGCTGGAACGGCGGCTGGTA
CCTGCCCGCCATGGCCCTGCCCTCGCCGAATTTCGGGCCCCGCCCCGCCCAGGCCGCCATCGATCTCATCGTGGTGCATT
CCATCAGCCTGCCCCCGGGCGAATACGGCACCGGCGAAGTCCAGCGCCTCTTCACCAACGCGCTGGACTGGGACGCCCAC
CCCTACTACGCCGGCATCCGCGGCCTGCAGGTATCGGCCCACTTCTTCATCGAGCGCGGCGGGCGGCTGTGGCAGTTCGT
GGACTGCGGCGACCGCGCATGGCACGCGGGCCCATCCGCCTACCGGGGCCGGGACAACTGCAACGACGACTCGATCGGCA
TCGAACTGGAAGGACTGGAGGGCGACACCTTCGAGCCCGCCCAGTACCAGGCGCTCGCCACGCTGTGCCGGGACATCGCG
CAACGCTATCCCGTGGCCCACGTCGCGGGCCATGAGCATGTCGCGCCCGGACGCAAGCGGGACCCCGGGCCGGGGTTCGA
CTGGCGGGAGCTGCGGTCCCGGCTGGGCAGCCCGCGCATCGACTTCCCGCCGCAAGTCAAGCCCTCCTGA

Upstream 100 bases:

>100_bases
GCCACCGCCGCCCGCCTGGGCATCAGCCTGCGGCAGATCCGCTACCGCATCGACCGCCTGGCCATCGCCACGCCGCAGGA
CGACGACGCGCCGGACGACC

Downstream 100 bases:

>100_bases
CCTGCGAGCAAGGGTTTTCCCGAGGTGCTGGCGCGGACGGGGGCAAATCGCGACACTCGCTCCCCTTGCGCTCCGCCAGC
ACTGGCGCCGGCCCGCGCCG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MQPPDPNDAPGHGGPDEAGPRWNGGWYLPAMALPSPNFGPRPAQAAIDLIVVHSISLPPGEYGTGEVQRLFTNALDWDAH
PYYAGIRGLQVSAHFFIERGGRLWQFVDCGDRAWHAGPSAYRGRDNCNDDSIGIELEGLEGDTFEPAQYQALATLCRDIA
QRYPVAHVAGHEHVAPGRKRDPGPGFDWRELRSRLGSPRIDFPPQVKPS

Sequences:

>Translated_209_residues
MQPPDPNDAPGHGGPDEAGPRWNGGWYLPAMALPSPNFGPRPAQAAIDLIVVHSISLPPGEYGTGEVQRLFTNALDWDAH
PYYAGIRGLQVSAHFFIERGGRLWQFVDCGDRAWHAGPSAYRGRDNCNDDSIGIELEGLEGDTFEPAQYQALATLCRDIA
QRYPVAHVAGHEHVAPGRKRDPGPGFDWRELRSRLGSPRIDFPPQVKPS
>Mature_209_residues
MQPPDPNDAPGHGGPDEAGPRWNGGWYLPAMALPSPNFGPRPAQAAIDLIVVHSISLPPGEYGTGEVQRLFTNALDWDAH
PYYAGIRGLQVSAHFFIERGGRLWQFVDCGDRAWHAGPSAYRGRDNCNDDSIGIELEGLEGDTFEPAQYQALATLCRDIA
QRYPVAHVAGHEHVAPGRKRDPGPGFDWRELRSRLGSPRIDFPPQVKPS

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=170, Percent_Identity=48.8235294117647, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1787092, Length=148, Percent_Identity=39.8648648648649, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 22939; Mature: 22939

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPPDPNDAPGHGGPDEAGPRWNGGWYLPAMALPSPNFGPRPAQAAIDLIVVHSISLPPG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHEEEEEEECCCCCC
EYGTGEVQRLFTNALDWDAHPYYAGIRGLQVSAHFFIERGGRLWQFVDCGDRAWHAGPSA
CCCCHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
YRGRDNCNDDSIGIELEGLEGDTFEPAQYQALATLCRDIAQRYPVAHVAGHEHVAPGRKR
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
DPGPGFDWRELRSRLGSPRIDFPPQVKPS
CCCCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPPDPNDAPGHGGPDEAGPRWNGGWYLPAMALPSPNFGPRPAQAAIDLIVVHSISLPPG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHEEEEEEECCCCCC
EYGTGEVQRLFTNALDWDAHPYYAGIRGLQVSAHFFIERGGRLWQFVDCGDRAWHAGPSA
CCCCHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
YRGRDNCNDDSIGIELEGLEGDTFEPAQYQALATLCRDIAQRYPVAHVAGHEHVAPGRKR
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
DPGPGFDWRELRSRLGSPRIDFPPQVKPS
CCCCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]