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The map label for this gene is pilR [H]

Identifier: 120609560

GI number: 120609560

Start: 939424

End: 940914

Strand: Reverse

Name: pilR [H]

Synonym: Aave_0866

Alternate gene names: 120609560

Gene position: 940914-939424 (Counterclockwise)

Preceding gene: 120609561

Following gene: 120609559

Centisome position: 17.58

GC content: 73.57

Gene sequence:

>1491_bases
ATGAATGCCCCCGCTGCCACCATCCTGGTCGTCGATGACGAACCCGACCTGCGCACGCTCTACGAACTCACGCTGCTGCG
CGAGGGCTACCGCGTGGAGACGGCGGCGACCGTCGAAGAGGCCCGCCAGCAGCTCCAGACCCGGCGTTTCGACGCCGTCA
TCACCGACATGCGCCTGCCGGACGGCTTCGGCATGGAGCTGCTGCAGGACATCCGCCAGCAGAAGCGGCGCGAGCGCTGC
GTGGTGATGACCGCCTACGGCTCCGCGGAGAACGCCGTCGAAGCGCTGCGCTCCGGCGCGTTCGACTACCTGACCAAGCC
GGTGGACCTCAAGCAGTTCCGCTCCGTGGTCGCCTCGGCCGTACAGGGCACGGGTGGCGTGCCGCCGCCCCGCTCCGCGC
GGCCCGGCCCGCCGGGGGCCGCCGCGGCCGCCAGCCTTTCCGGGCTCGATCCGCTCGTGGGCGACTCGGAGGCCATGCGC
AACGTCAAGCAGCGCATCGCCAAGGTCGCGCGCAGCATGGCTCCCGTGCTGATCCGCGGCGAATCGGGCACCGGCAAGGA
ACTCGTCGCCCGCGCCCTGCACGGCTGCAGCCAGCGCGCCGACGGCCCGCTGGTGGCCGTGAACTGCGGCGCCATCCCCG
AGAACCTGCTGGAGGCGGAATTCTTCGGCGCGCGCAAGGGTTCCTACACCGGCGCCAGCCAGGACCGGGACGGCTACTTC
CAGGCCGCCCGCGGGGGCACGCTGTTCCTCGACGAGATCGGCGACCTGCCCCTGTCCATGCAGGCCAAGCTGCTGCGCGC
CATCCAGGAGCGCAGCGTGCGCCCGCTGGGGTCCACCCAGGAAGACACCGTGGACGTGCGGATCGTCAGCGCCACCCACC
GCGACCTGGCCGCCGACGTGCAGGCGGGCCGCTTCCGGCAGGACCTCTACTACCGCCTCAACGTGATCGAGGTCGTGATC
CCGCCGCTGCGCGAGCGCCGCGAGGATCTTCCGGCCCTGTGCGCCGCGCTGCTGGACCGCATCGCCGCGGAAACCGGCCT
GCCCGTGCCCGCGCTCACCGGCGAGGCCCTGGCGGACATCGCCCGGCACCCGCTCACCGGCAATGTGCGCGAACTGGAGA
ACCTGCTGCACCGCGCCGTGGCGCTGAGCGACGGCGGCGAACTGCACATCGAATGCCCCGCGGATGCCGCTGCCGGCACG
CCGCGCGCGCCGGTGGCCGCGGCCCCGGCGCCCGAGGCGTCCGGCGCGCCTGCCGGCACCGCGGACCCGCTGCCCGCGGC
CCACGTGCCCCTGCCCAGCGACCTGCAGGGCTGGCTGGACCAGCAGGAGCGCGAGATCCTGGTGCGCGCCCTGCGCGAGC
ACGGGTTCAACCGCACGGCCACCGCCGCCCGCCTGGGCATCAGCCTGCGGCAGATCCGCTACCGCATCGACCGCCTGGCC
ATCGCCACGCCGCAGGACGACGACGCGCCGGACGACCATGCAGCCTCCTGA

Upstream 100 bases:

>100_bases
GCAACGCATTCACGGTGGCCTTCCGCCGCGCCGCCAGGCCGGGAGGACCGGCCTCCCTGTTTGACACAATCGTCGTCTGA
CACCGCCCGACCATGCCGCC

Downstream 100 bases:

>100_bases
CCCGAACGACGCTCCCGGGCACGGCGGCCCGGATGAAGCCGGCCCGCGCTGGAACGGCGGCTGGTACCTGCCCGCCATGG
CCCTGCCCTCGCCGAATTTC

Product: Fis family two component sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNAPAATILVVDDEPDLRTLYELTLLREGYRVETAATVEEARQQLQTRRFDAVITDMRLPDGFGMELLQDIRQQKRRERC
VVMTAYGSAENAVEALRSGAFDYLTKPVDLKQFRSVVASAVQGTGGVPPPRSARPGPPGAAAAASLSGLDPLVGDSEAMR
NVKQRIAKVARSMAPVLIRGESGTGKELVARALHGCSQRADGPLVAVNCGAIPENLLEAEFFGARKGSYTGASQDRDGYF
QAARGGTLFLDEIGDLPLSMQAKLLRAIQERSVRPLGSTQEDTVDVRIVSATHRDLAADVQAGRFRQDLYYRLNVIEVVI
PPLRERREDLPALCAALLDRIAAETGLPVPALTGEALADIARHPLTGNVRELENLLHRAVALSDGGELHIECPADAAAGT
PRAPVAAAPAPEASGAPAGTADPLPAAHVPLPSDLQGWLDQQEREILVRALREHGFNRTATAARLGISLRQIRYRIDRLA
IATPQDDDAPDDHAAS

Sequences:

>Translated_496_residues
MNAPAATILVVDDEPDLRTLYELTLLREGYRVETAATVEEARQQLQTRRFDAVITDMRLPDGFGMELLQDIRQQKRRERC
VVMTAYGSAENAVEALRSGAFDYLTKPVDLKQFRSVVASAVQGTGGVPPPRSARPGPPGAAAAASLSGLDPLVGDSEAMR
NVKQRIAKVARSMAPVLIRGESGTGKELVARALHGCSQRADGPLVAVNCGAIPENLLEAEFFGARKGSYTGASQDRDGYF
QAARGGTLFLDEIGDLPLSMQAKLLRAIQERSVRPLGSTQEDTVDVRIVSATHRDLAADVQAGRFRQDLYYRLNVIEVVI
PPLRERREDLPALCAALLDRIAAETGLPVPALTGEALADIARHPLTGNVRELENLLHRAVALSDGGELHIECPADAAAGT
PRAPVAAAPAPEASGAPAGTADPLPAAHVPLPSDLQGWLDQQEREILVRALREHGFNRTATAARLGISLRQIRYRIDRLA
IATPQDDDAPDDHAAS
>Mature_496_residues
MNAPAATILVVDDEPDLRTLYELTLLREGYRVETAATVEEARQQLQTRRFDAVITDMRLPDGFGMELLQDIRQQKRRERC
VVMTAYGSAENAVEALRSGAFDYLTKPVDLKQFRSVVASAVQGTGGVPPPRSARPGPPGAAAAASLSGLDPLVGDSEAMR
NVKQRIAKVARSMAPVLIRGESGTGKELVARALHGCSQRADGPLVAVNCGAIPENLLEAEFFGARKGSYTGASQDRDGYF
QAARGGTLFLDEIGDLPLSMQAKLLRAIQERSVRPLGSTQEDTVDVRIVSATHRDLAADVQAGRFRQDLYYRLNVIEVVI
PPLRERREDLPALCAALLDRIAAETGLPVPALTGEALADIARHPLTGNVRELENLLHRAVALSDGGELHIECPADAAAGT
PRAPVAAAPAPEASGAPAGTADPLPAAHVPLPSDLQGWLDQQEREILVRALREHGFNRTATAARLGISLRQIRYRIDRLA
IATPQDDDAPDDHAAS

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=471, Percent_Identity=42.0382165605096, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1788550, Length=477, Percent_Identity=38.7840670859539, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788905, Length=384, Percent_Identity=43.2291666666667, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1790299, Length=460, Percent_Identity=38.695652173913, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=40.9356725146199, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=331, Percent_Identity=39.8791540785499, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=39.9408284023669, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87082152, Length=348, Percent_Identity=38.2183908045977, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=243, Percent_Identity=44.8559670781893, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI87081872, Length=328, Percent_Identity=36.890243902439, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1787583, Length=248, Percent_Identity=41.5322580645161, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=35.6846473029046, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI87081858, Length=223, Percent_Identity=35.4260089686099, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786911, Length=125, Percent_Identity=30.4, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53248; Mature: 53248

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPAATILVVDDEPDLRTLYELTLLREGYRVETAATVEEARQQLQTRRFDAVITDMRLP
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGFGMELLQDIRQQKRRERCVVMTAYGSAENAVEALRSGAFDYLTKPVDLKQFRSVVASA
CCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
VQGTGGVPPPRSARPGPPGAAAAASLSGLDPLVGDSEAMRNVKQRIAKVARSMAPVLIRG
HHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
ESGTGKELVARALHGCSQRADGPLVAVNCGAIPENLLEAEFFGARKGSYTGASQDRDGYF
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
QAARGGTLFLDEIGDLPLSMQAKLLRAIQERSVRPLGSTQEDTVDVRIVSATHRDLAADV
HHCCCCEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHHH
QAGRFRQDLYYRLNVIEVVIPPLRERREDLPALCAALLDRIAAETGLPVPALTGEALADI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ARHPLTGNVRELENLLHRAVALSDGGELHIECPADAAAGTPRAPVAAAPAPEASGAPAGT
HHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADPLPAAHVPLPSDLQGWLDQQEREILVRALREHGFNRTATAARLGISLRQIRYRIDRLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHE
IATPQDDDAPDDHAAS
EECCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPAATILVVDDEPDLRTLYELTLLREGYRVETAATVEEARQQLQTRRFDAVITDMRLP
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGFGMELLQDIRQQKRRERCVVMTAYGSAENAVEALRSGAFDYLTKPVDLKQFRSVVASA
CCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
VQGTGGVPPPRSARPGPPGAAAAASLSGLDPLVGDSEAMRNVKQRIAKVARSMAPVLIRG
HHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
ESGTGKELVARALHGCSQRADGPLVAVNCGAIPENLLEAEFFGARKGSYTGASQDRDGYF
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
QAARGGTLFLDEIGDLPLSMQAKLLRAIQERSVRPLGSTQEDTVDVRIVSATHRDLAADV
HHCCCCEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHHH
QAGRFRQDLYYRLNVIEVVIPPLRERREDLPALCAALLDRIAAETGLPVPALTGEALADI
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ARHPLTGNVRELENLLHRAVALSDGGELHIECPADAAAGTPRAPVAAAPAPEASGAPAGT
HHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADPLPAAHVPLPSDLQGWLDQQEREILVRALREHGFNRTATAARLGISLRQIRYRIDRLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHE
IATPQDDDAPDDHAAS
EECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]