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The map label for this gene is gatA2 [H]

Identifier: 120609543

GI number: 120609543

Start: 919355

End: 920728

Strand: Reverse

Name: gatA2 [H]

Synonym: Aave_0849

Alternate gene names: 120609543

Gene position: 920728-919355 (Counterclockwise)

Preceding gene: 120609548

Following gene: 120609542

Centisome position: 17.2

GC content: 75.55

Gene sequence:

>1374_bases
ATGCCCGACCGCCCCGCCCTTCCCACGATCCGCGAACTCGCCCGCTCCCTGGCCGAGGGCGCCACGACGAGCGAGGCCCT
GGTGGAGGCGGCACTGGAGCGCATCGCGGCGCACCGTGCCGGCGGAGGCGCCGCCTACGTCGGCGAAGTGGACGCGCTGG
CCGCCCGGCTGGCGGCACGGGCGAGCGACCTCGCCCGTGCCGCGGGCCACGTGCCGTCTCCGCTCGCGGGCCTGCCGGTG
TCGGTCAAGGACCTGTTCGATGTGCGCGGACAGGTGACGCGCGCCGGCTCCGCCGTGCTGGCGGATGCCGGGCCCGCCCC
GAGCGATGCGGCCGCCGTGGCCCGGCTGCGCGCCGCGGGGGCCGTCCTGCTGGGCCGCACCAACATGAGCGAGTTCGCCT
TTTCCGGGCTGGGCCTCAACCCCCACCACGGCACGCCGGCGCATCCACAGGATGCCGGCCGGGTCACGGGTGGATCCAGC
TCCGGCGCGGCGGCCACGGTAGCGCTGGACCTGGCCGCTGCGGCGCTGGGTACGGACACGGGCGGCTCCATCCGCATTCC
GTCGGCGTTCTGCGGGCTCACGGGCTTCAAGCCGACCGCACGGCGGGTGCCGCTCGCGGGCGCGTATCCGCTCTCGCGCA
GCCTCGACTCCGTCGGCCCGCTGGCGCGCAGCGTGGATTGCTGCGCGATCCTGGACGCGGTGCTGTCGGGCGAGGGAAGC
GACGCCGCCCCTGCCGGCGCGTGGCCGCTGGCCGGGATGCGGCTGGGGGTCGTGCAGGATCTCGTCATGGACGGCCTGGA
GCCCGAGGTGGCCGCCGCGTTCGAGGACGCGCTGCGCCGGCTTTCCGCGGCAGGGGCCCGCATCGAACGCGTGGCCTTTG
CGGAACTGCACGCGCTGCCGCGCATCAACGCGGCGGGCGGGCTGATCGCGGCGGAGGCCTGGCAGGTGCACCGGGCACGC
CTCGTGGACGCCTCCGTGGCGGCCCGCTACGACCCGCGCGTGGCGCAGCGCACCCGGCGTGGGGACGCCATCCTGGCAGC
GGACTACATCGATGTGCAGGATGCCCGCGTCCGGCTGCAGGCCGCCGCGCAGGAGCGACTGGGCCACCTGGACGCCTGGC
TGATGCCCACGGTGGCGGTGCGTGCGCCGCTGCGCGAGCCGCTGGAGCAGGACGACGCCCACTTCTTCGCGACCAACGCC
CTGGTGCTGCGCAATACCTCGGTGATCAATTTCCTGGACGGCTGCGCGCTGTCGCTGCCCTGCGGGGCCCCGGACGAACT
GCCAGTGGGCCTGTCGGTGGCGGGGCTGCACGGCGCGGATGCCCGCATCCTGCAGGTGGGCCGCGCCGTGGAGGCGTGCC
TGCGCGCGGACTAA

Upstream 100 bases:

>100_bases
CTCTCGGAACCTGCCCGGGGTGCGGCGCCGGCCTGCAAGAATGCACGGTCAGCCCTTTCCCTGCTTCATCCAACCGGCCC
AGCCCAGGAGACCGCCGTCC

Downstream 100 bases:

>100_bases
GAGCGGCTAACACCACCGACTGAGACCTGCGCAGAGCCGTTATGCTTGGCGCCCTCATGGCAAGAAGACCCGTCAGCAAA
GAACTGTGGCGACAGCTGCA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MPDRPALPTIRELARSLAEGATTSEALVEAALERIAAHRAGGGAAYVGEVDALAARLAARASDLARAAGHVPSPLAGLPV
SVKDLFDVRGQVTRAGSAVLADAGPAPSDAAAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHHGTPAHPQDAGRVTGGSS
SGAAATVALDLAAAALGTDTGGSIRIPSAFCGLTGFKPTARRVPLAGAYPLSRSLDSVGPLARSVDCCAILDAVLSGEGS
DAAPAGAWPLAGMRLGVVQDLVMDGLEPEVAAAFEDALRRLSAAGARIERVAFAELHALPRINAAGGLIAAEAWQVHRAR
LVDASVAARYDPRVAQRTRRGDAILAADYIDVQDARVRLQAAAQERLGHLDAWLMPTVAVRAPLREPLEQDDAHFFATNA
LVLRNTSVINFLDGCALSLPCGAPDELPVGLSVAGLHGADARILQVGRAVEACLRAD

Sequences:

>Translated_457_residues
MPDRPALPTIRELARSLAEGATTSEALVEAALERIAAHRAGGGAAYVGEVDALAARLAARASDLARAAGHVPSPLAGLPV
SVKDLFDVRGQVTRAGSAVLADAGPAPSDAAAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHHGTPAHPQDAGRVTGGSS
SGAAATVALDLAAAALGTDTGGSIRIPSAFCGLTGFKPTARRVPLAGAYPLSRSLDSVGPLARSVDCCAILDAVLSGEGS
DAAPAGAWPLAGMRLGVVQDLVMDGLEPEVAAAFEDALRRLSAAGARIERVAFAELHALPRINAAGGLIAAEAWQVHRAR
LVDASVAARYDPRVAQRTRRGDAILAADYIDVQDARVRLQAAAQERLGHLDAWLMPTVAVRAPLREPLEQDDAHFFATNA
LVLRNTSVINFLDGCALSLPCGAPDELPVGLSVAGLHGADARILQVGRAVEACLRAD
>Mature_456_residues
PDRPALPTIRELARSLAEGATTSEALVEAALERIAAHRAGGGAAYVGEVDALAARLAARASDLARAAGHVPSPLAGLPVS
VKDLFDVRGQVTRAGSAVLADAGPAPSDAAAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHHGTPAHPQDAGRVTGGSSS
GAAATVALDLAAAALGTDTGGSIRIPSAFCGLTGFKPTARRVPLAGAYPLSRSLDSVGPLARSVDCCAILDAVLSGEGSD
AAPAGAWPLAGMRLGVVQDLVMDGLEPEVAAAFEDALRRLSAAGARIERVAFAELHALPRINAAGGLIAAEAWQVHRARL
VDASVAARYDPRVAQRTRRGDAILAADYIDVQDARVRLQAAAQERLGHLDAWLMPTVAVRAPLREPLEQDDAHFFATNAL
VLRNTSVINFLDGCALSLPCGAPDELPVGLSVAGLHGADARILQVGRAVEACLRAD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=26.9461077844311, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI195972892, Length=239, Percent_Identity=31.3807531380753, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI166795287, Length=156, Percent_Identity=39.1025641025641, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=300, Percent_Identity=28.3333333333333, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=476, Percent_Identity=26.6806722689076, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=228, Percent_Identity=34.2105263157895, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=204, Percent_Identity=34.3137254901961, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=225, Percent_Identity=30.6666666666667, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=236, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=236, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=197, Percent_Identity=32.48730964467, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=384, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=26.6509433962264, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648113, Length=514, Percent_Identity=28.5992217898833, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=23.1237322515213, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161078093, Length=488, Percent_Identity=25.8196721311475, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550774, Length=228, Percent_Identity=30.7017543859649, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648435, Length=228, Percent_Identity=30.7017543859649, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648437, Length=228, Percent_Identity=30.7017543859649, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356731, Length=228, Percent_Identity=32.0175438596491, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652985, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922090, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652981, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652983, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648441, Length=140, Percent_Identity=36.4285714285714, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648439, Length=140, Percent_Identity=36.4285714285714, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 46753; Mature: 46621

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRPALPTIRELARSLAEGATTSEALVEAALERIAAHRAGGGAAYVGEVDALAARLAAR
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
ASDLARAAGHVPSPLAGLPVSVKDLFDVRGQVTRAGSAVLADAGPAPSDAAAVARLRAAG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHC
AVLLGRTNMSEFAFSGLGLNPHHGTPAHPQDAGRVTGGSSSGAAATVALDLAAAALGTDT
EEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
GGSIRIPSAFCGLTGFKPTARRVPLAGAYPLSRSLDSVGPLARSVDCCAILDAVLSGEGS
CCCEECCHHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DAAPAGAWPLAGMRLGVVQDLVMDGLEPEVAAAFEDALRRLSAAGARIERVAFAELHALP
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
RINAAGGLIAAEAWQVHRARLVDASVAARYDPRVAQRTRRGDAILAADYIDVQDARVRLQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCHHHHHHHHH
AAAQERLGHLDAWLMPTVAVRAPLREPLEQDDAHFFATNALVLRNTSVINFLDGCALSLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCHHHHHHCCCEEECC
CGAPDELPVGLSVAGLHGADARILQVGRAVEACLRAD
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDRPALPTIRELARSLAEGATTSEALVEAALERIAAHRAGGGAAYVGEVDALAARLAAR
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHH
ASDLARAAGHVPSPLAGLPVSVKDLFDVRGQVTRAGSAVLADAGPAPSDAAAVARLRAAG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHC
AVLLGRTNMSEFAFSGLGLNPHHGTPAHPQDAGRVTGGSSSGAAATVALDLAAAALGTDT
EEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
GGSIRIPSAFCGLTGFKPTARRVPLAGAYPLSRSLDSVGPLARSVDCCAILDAVLSGEGS
CCCEECCHHHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DAAPAGAWPLAGMRLGVVQDLVMDGLEPEVAAAFEDALRRLSAAGARIERVAFAELHALP
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
RINAAGGLIAAEAWQVHRARLVDASVAARYDPRVAQRTRRGDAILAADYIDVQDARVRLQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCHHHHHHHHH
AAAQERLGHLDAWLMPTVAVRAPLREPLEQDDAHFFATNALVLRNTSVINFLDGCALSLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCHHHHHHCCCEEECC
CGAPDELPVGLSVAGLHGADARILQVGRAVEACLRAD
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]