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The map label for this gene is mpl [H]

Identifier: 120609548

GI number: 120609548

Start: 924806

End: 926227

Strand: Reverse

Name: mpl [H]

Synonym: Aave_0854

Alternate gene names: 120609548

Gene position: 926227-924806 (Counterclockwise)

Preceding gene: 120609556

Following gene: 120609543

Centisome position: 17.3

GC content: 71.94

Gene sequence:

>1422_bases
ATGCACATTCACATTCTGGGCATCTGCGGAACGTTCATGGGAGGCCTGGCCGCGCTGGCGCGGGAGGCGGGCCACAAGGT
CACGGGCTGCGATGCCGGGGTGTATCCGCCGATGAGCGACCAGCTGCGCGCGCTGGGCATCGAATTGATCGAAGGCTACG
GCGCCGAGCAGCTGGACCTGGCGCCGGACATGTTCGTCGTGGGCAACGTGGTGAGCCGCGCGCGGCTTCCCGACGGCGGC
CCCAAGTTCCCCCTGATGGAAGCCATCCTGGACGCGGGCGCCCCCTACACGAGCGGCCCGCAGTGGCTGGCCGAGCACGT
GCTGCAGGGGCGCCACGTGCTCGCGGTGGCGGGCACGCACGGCAAGACCACGACCACGTCCATGCTCGCCTGGGTCCTCG
AATGCGCGGGCCTGCAGCCCGGCTTCCTGGTGGGCGGCGTTCCCCTGGATTTCGGTGTCTCCGCCCGGCTGGGCGCGGCG
CAGCGGCCGGTGGCGCAGGACGGCCCGGCCGGCGGCGCGCCGCTCTTCGTGATCGAGGCCGACGAATACGACACGGCCTT
CTTCGACAAGCGCAGCAAGTTCGTCCACTACCGCCCGCGCACCGCGGTGCTGAACAACCTCGAGTTCGACCACGCGGACA
TCTTCGACGACCTGGCCGCGATCGAGCGGCAGTTCCACCACCTCGTGCGCACGGTGCCGCCTTCGGGCCGGGTGGTGTTC
AACGGGCTGGAGGAAAGCCTGGCACGGGTGCTGCACCAGGGCTGCTGGAGCGGCGTGGCCAGCTTCGGTGCGGCGGTGAG
CGATTTCACGGCGCGCGGCGAGCCGCAGGCCTTCGACGTGCTGCGCGGCGGCCATGCCGTCGGCCGCGTGGAGTGGGAAC
TCAGCGGCGTGCACAACCAGCTCAACGCGCTGGCGGCCATCGCGGCCGCGGACCACGTGGGCGTGGCGCCCGCCGAGGCC
GCGGCGGCGCTGGGACGATTCCGCAACGTCAAGCGGCGCATGGAACTGCGCGGCACGGCCGGCGGCGTCGCGGTGTATGA
CGATTTCGCGCACCATCCCACGGCGCTGCGCACCACGCTGGACGGGCTGCGCCGCCGCGTCGGGCCGGATGCACGCATCC
TCGCGGCCTTCGAACCGCGCAGCAACACGATGAAGCTCGGCACGATGAAGGCGCAGCTGCCGTGGGCCCTGGAAGCCGCC
GACCTCGCCTTCTGCCACACGGCAGGGCTGGACTGGGACGCAGCCGAGGCGCTCGCCCCGCTGGGGAGCAGGGCCTGCAC
GGCAGGCGACATCGCCACCCTGGTGGAACGGATCACCGAGGCGGCGCGTCCGGGCGACCATGTGGTGTGCATGAGCAACG
GCAGCTTCGGCGGCATCCACGCCCGGCTGCTGGGCGCCCTGGCGGAGCGCGCCGGTCAGTAG

Upstream 100 bases:

>100_bases
GGGCCACGCCTGCCGTGCCGACGGTGCATGGCTCCGGAAAGAATGGCAGCCCCCGTCCTTCCCGCGGTCATGCGGGAGCC
GAACCGTAGAATCGGCGCCC

Downstream 100 bases:

>100_bases
CTGATCGCCATCGCCGGCAGATCGACGCGCACGACCGGCCGGGCCAGCGCCGCGGACGCCGCCGCGGCGAACCGGCGGCC
CCAGTCCTGCGCCAGCAGGG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MHIHILGICGTFMGGLAALAREAGHKVTGCDAGVYPPMSDQLRALGIELIEGYGAEQLDLAPDMFVVGNVVSRARLPDGG
PKFPLMEAILDAGAPYTSGPQWLAEHVLQGRHVLAVAGTHGKTTTTSMLAWVLECAGLQPGFLVGGVPLDFGVSARLGAA
QRPVAQDGPAGGAPLFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQFHHLVRTVPPSGRVVF
NGLEESLARVLHQGCWSGVASFGAAVSDFTARGEPQAFDVLRGGHAVGRVEWELSGVHNQLNALAAIAAADHVGVAPAEA
AAALGRFRNVKRRMELRGTAGGVAVYDDFAHHPTALRTTLDGLRRRVGPDARILAAFEPRSNTMKLGTMKAQLPWALEAA
DLAFCHTAGLDWDAAEALAPLGSRACTAGDIATLVERITEAARPGDHVVCMSNGSFGGIHARLLGALAERAGQ

Sequences:

>Translated_473_residues
MHIHILGICGTFMGGLAALAREAGHKVTGCDAGVYPPMSDQLRALGIELIEGYGAEQLDLAPDMFVVGNVVSRARLPDGG
PKFPLMEAILDAGAPYTSGPQWLAEHVLQGRHVLAVAGTHGKTTTTSMLAWVLECAGLQPGFLVGGVPLDFGVSARLGAA
QRPVAQDGPAGGAPLFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQFHHLVRTVPPSGRVVF
NGLEESLARVLHQGCWSGVASFGAAVSDFTARGEPQAFDVLRGGHAVGRVEWELSGVHNQLNALAAIAAADHVGVAPAEA
AAALGRFRNVKRRMELRGTAGGVAVYDDFAHHPTALRTTLDGLRRRVGPDARILAAFEPRSNTMKLGTMKAQLPWALEAA
DLAFCHTAGLDWDAAEALAPLGSRACTAGDIATLVERITEAARPGDHVVCMSNGSFGGIHARLLGALAERAGQ
>Mature_473_residues
MHIHILGICGTFMGGLAALAREAGHKVTGCDAGVYPPMSDQLRALGIELIEGYGAEQLDLAPDMFVVGNVVSRARLPDGG
PKFPLMEAILDAGAPYTSGPQWLAEHVLQGRHVLAVAGTHGKTTTTSMLAWVLECAGLQPGFLVGGVPLDFGVSARLGAA
QRPVAQDGPAGGAPLFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQFHHLVRTVPPSGRVVF
NGLEESLARVLHQGCWSGVASFGAAVSDFTARGEPQAFDVLRGGHAVGRVEWELSGVHNQLNALAAIAAADHVGVAPAEA
AAALGRFRNVKRRMELRGTAGGVAVYDDFAHHPTALRTTLDGLRRRVGPDARILAAFEPRSNTMKLGTMKAQLPWALEAA
DLAFCHTAGLDWDAAEALAPLGSRACTAGDIATLVERITEAARPGDHVVCMSNGSFGGIHARLLGALAERAGQ

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=475, Percent_Identity=54.3157894736842, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1786279, Length=398, Percent_Identity=30.4020100502513, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50009; Mature: 50009

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAALAREAGHKVTGCDAGVYPPMSDQLRALGIELIEGYGAEQLDL
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
APDMFVVGNVVSRARLPDGGPKFPLMEAILDAGAPYTSGPQWLAEHVLQGRHVLAVAGTH
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCC
GKTTTTSMLAWVLECAGLQPGFLVGGVPLDFGVSARLGAAQRPVAQDGPAGGAPLFVIEA
CCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
DEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQFHHLVRTVPPSGRVVF
CCCCHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
NGLEESLARVLHQGCWSGVASFGAAVSDFTARGEPQAFDVLRGGHAVGRVEWELSGVHNQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEHHHHHHH
LNALAAIAAADHVGVAPAEAAAALGRFRNVKRRMELRGTAGGVAVYDDFAHHPTALRTTL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHH
DGLRRRVGPDARILAAFEPRSNTMKLGTMKAQLPWALEAADLAFCHTAGLDWDAAEALAP
HHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHHHCCCCCCHHHHHHH
LGSRACTAGDIATLVERITEAARPGDHVVCMSNGSFGGIHARLLGALAERAGQ
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAALAREAGHKVTGCDAGVYPPMSDQLRALGIELIEGYGAEQLDL
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
APDMFVVGNVVSRARLPDGGPKFPLMEAILDAGAPYTSGPQWLAEHVLQGRHVLAVAGTH
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCC
GKTTTTSMLAWVLECAGLQPGFLVGGVPLDFGVSARLGAAQRPVAQDGPAGGAPLFVIEA
CCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
DEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQFHHLVRTVPPSGRVVF
CCCCHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
NGLEESLARVLHQGCWSGVASFGAAVSDFTARGEPQAFDVLRGGHAVGRVEWELSGVHNQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEHHHHHHH
LNALAAIAAADHVGVAPAEAAAALGRFRNVKRRMELRGTAGGVAVYDDFAHHPTALRTTL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHH
DGLRRRVGPDARILAAFEPRSNTMKLGTMKAQLPWALEAADLAFCHTAGLDWDAAEALAP
HHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHHHCCCCCCHHHHHHH
LGSRACTAGDIATLVERITEAARPGDHVVCMSNGSFGGIHARLLGALAERAGQ
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]