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The map label for this gene is rlmI [H]

Identifier: 120609499

GI number: 120609499

Start: 869362

End: 870549

Strand: Direct

Name: rlmI [H]

Synonym: Aave_0805

Alternate gene names: 120609499

Gene position: 869362-870549 (Clockwise)

Preceding gene: 120609498

Following gene: 120609501

Centisome position: 16.24

GC content: 70.88

Gene sequence:

>1188_bases
ATGAAAACCATCCGCCTGCGCCCTGGCAAGGAGCGTTCCCTCCTGCGCCGCCATCCCTGGATCTTCGAATCGGCCATCGC
CAAAGGCGGTGGGGACGGCGGCGAAACCGTGCGGGTGGAGTCGGCGGACGGCCGCTTCCTGGCCTGGGCCGCGTTCAGCC
CGGCATCGCGCATCCGTGCCCGCGCCTGGAGCTTCGACGAGGCGCAGCGCATCGACCCCGCGTTCTTCGCCGCGGCCGTG
GAGCGCGCGGTCCGGGCACGGGAACGCTTCGCCATCGAGAGCGACGGCGTGCGCCTGGTGCATGGGGAATCCGACGGCCT
GCCCGGCCTGGTCGTGGACCGCTACGGCGACACCCTGGTGGCGCAGTTCACCAGCGCCGGCACCGAGCGCTGGAAGGCCG
TCCTCGCCGATGCCCTGCTGAAGGCCACCGGCCTGCAGAAGCTCTACGAACGTTCCGACGCCAGCGTGCGCCAGCTGGAG
GGCCTGGAGCCCGCCACGGGCTGGCTGCGCGGCGAAGGCCCCACGGAGCTGTCCATCCGCGAACATGGCTGGCGCCTCAC
CCTGGACATCGCCGAAGGCCACAAGACCGGCTTCTACCTCGACCAGCGCGACAGCCGCCACCGCTTCTCCGAGGCCGTTC
GCCGGCTCGGCGCGCGCCAGGTGCTCAACTGCTACTGCTACACCGGCGGCTTCACCGTCGCGGCGCTCGCGGGCGGAGCC
TCGCAGGTGACCTCCATCGATTCGTCGGGACCGGCGCTGGAACGCGCGCGCTCGCATGCCGCCCTCAACGGCTTCGACGC
CGCCCGCACGGAGTTCCTCGATGCCGACGTGAACGCCTCCCTGCGGCGCTTCCTGCAGGAGGGCCGCCGCTTCGACGCCA
TCGTGCTCGATCCGCCGAAGTTCGCCCCCACGGCCGCGCATGCCGAGCGCGCCGCCCGGGCCTACAAGGACATCAACCGC
CTGGGCCTGCAATTGCTGTCTCCCGGCGGATTGCTCTATACCTTTTCCTGCTCCGGTGGCATCAGCGCCGACCTGTTCCA
CAAGATCGTGGCCTCGGCCGGCGCCGACGCGGGCGTGGACGGCTACATCCTCGAGCGCCTGGGCGGCGCGCCCGACCATC
CCATGACCCTGGAATTCCCGGAGGGCGAATACCTCAAGGGCCTGGCGGTGATGCGCAAGGCAGCGTGA

Upstream 100 bases:

>100_bases
GCGCTCTACCCGGCGCGCGCAGCCACCGCGGAGCCTGCCGCCCGCTGAACCGCCCCGGATGCCGGTCCGGGCGCACCGCG
GCTCCCGGACAATACGGCCC

Downstream 100 bases:

>100_bases
CGGGCGCCTGCGGGCCGCTGCCTGCGCCGGCCATCTCGACGCGCCCGCGGCCGCCGCGCTTGGCGGCGTAGCAGGCCGCG
TCCGCATCGGCCATGAGCTT

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKTIRLRPGKERSLLRRHPWIFESAIAKGGGDGGETVRVESADGRFLAWAAFSPASRIRARAWSFDEAQRIDPAFFAAAV
ERAVRARERFAIESDGVRLVHGESDGLPGLVVDRYGDTLVAQFTSAGTERWKAVLADALLKATGLQKLYERSDASVRQLE
GLEPATGWLRGEGPTELSIREHGWRLTLDIAEGHKTGFYLDQRDSRHRFSEAVRRLGARQVLNCYCYTGGFTVAALAGGA
SQVTSIDSSGPALERARSHAALNGFDAARTEFLDADVNASLRRFLQEGRRFDAIVLDPPKFAPTAAHAERAARAYKDINR
LGLQLLSPGGLLYTFSCSGGISADLFHKIVASAGADAGVDGYILERLGGAPDHPMTLEFPEGEYLKGLAVMRKAA

Sequences:

>Translated_395_residues
MKTIRLRPGKERSLLRRHPWIFESAIAKGGGDGGETVRVESADGRFLAWAAFSPASRIRARAWSFDEAQRIDPAFFAAAV
ERAVRARERFAIESDGVRLVHGESDGLPGLVVDRYGDTLVAQFTSAGTERWKAVLADALLKATGLQKLYERSDASVRQLE
GLEPATGWLRGEGPTELSIREHGWRLTLDIAEGHKTGFYLDQRDSRHRFSEAVRRLGARQVLNCYCYTGGFTVAALAGGA
SQVTSIDSSGPALERARSHAALNGFDAARTEFLDADVNASLRRFLQEGRRFDAIVLDPPKFAPTAAHAERAARAYKDINR
LGLQLLSPGGLLYTFSCSGGISADLFHKIVASAGADAGVDGYILERLGGAPDHPMTLEFPEGEYLKGLAVMRKAA
>Mature_395_residues
MKTIRLRPGKERSLLRRHPWIFESAIAKGGGDGGETVRVESADGRFLAWAAFSPASRIRARAWSFDEAQRIDPAFFAAAV
ERAVRARERFAIESDGVRLVHGESDGLPGLVVDRYGDTLVAQFTSAGTERWKAVLADALLKATGLQKLYERSDASVRQLE
GLEPATGWLRGEGPTELSIREHGWRLTLDIAEGHKTGFYLDQRDSRHRFSEAVRRLGARQVLNCYCYTGGFTVAALAGGA
SQVTSIDSSGPALERARSHAALNGFDAARTEFLDADVNASLRRFLQEGRRFDAIVLDPPKFAPTAAHAERAARAYKDINR
LGLQLLSPGGLLYTFSCSGGISADLFHKIVASAGADAGVDGYILERLGGAPDHPMTLEFPEGEYLKGLAVMRKAA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=391, Percent_Identity=48.5933503836317, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1787181, Length=182, Percent_Identity=30.2197802197802, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 42958; Mature: 42958

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIRLRPGKERSLLRRHPWIFESAIAKGGGDGGETVRVESADGRFLAWAAFSPASRIRA
CCEEECCCCCHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHH
RAWSFDEAQRIDPAFFAAAVERAVRARERFAIESDGVRLVHGESDGLPGLVVDRYGDTLV
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCEEEECCCCHHH
AQFTSAGTERWKAVLADALLKATGLQKLYERSDASVRQLEGLEPATGWLRGEGPTELSIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCEEEEE
EHGWRLTLDIAEGHKTGFYLDQRDSRHRFSEAVRRLGARQVLNCYCYTGGFTVAALAGGA
CCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCC
SQVTSIDSSGPALERARSHAALNGFDAARTEFLDADVNASLRRFLQEGRRFDAIVLDPPK
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCC
FAPTAAHAERAARAYKDINRLGLQLLSPGGLLYTFSCSGGISADLFHKIVASAGADAGVD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
GYILERLGGAPDHPMTLEFPEGEYLKGLAVMRKAA
HHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIRLRPGKERSLLRRHPWIFESAIAKGGGDGGETVRVESADGRFLAWAAFSPASRIRA
CCEEECCCCCHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHH
RAWSFDEAQRIDPAFFAAAVERAVRARERFAIESDGVRLVHGESDGLPGLVVDRYGDTLV
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCEEEECCCCHHH
AQFTSAGTERWKAVLADALLKATGLQKLYERSDASVRQLEGLEPATGWLRGEGPTELSIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCEEEEE
EHGWRLTLDIAEGHKTGFYLDQRDSRHRFSEAVRRLGARQVLNCYCYTGGFTVAALAGGA
CCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCC
SQVTSIDSSGPALERARSHAALNGFDAARTEFLDADVNASLRRFLQEGRRFDAIVLDPPK
HHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCC
FAPTAAHAERAARAYKDINRLGLQLLSPGGLLYTFSCSGGISADLFHKIVASAGADAGVD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
GYILERLGGAPDHPMTLEFPEGEYLKGLAVMRKAA
HHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA