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The map label for this gene is pcrA [H]

Identifier: 120609372

GI number: 120609372

Start: 734279

End: 736078

Strand: Reverse

Name: pcrA [H]

Synonym: Aave_0675

Alternate gene names: 120609372

Gene position: 736078-734279 (Counterclockwise)

Preceding gene: 120609373

Following gene: 120609367

Centisome position: 13.75

GC content: 53.0

Gene sequence:

>1800_bases
ATGTTCACGTGGGACAAGGAAGATCTGAATCCGGAGCAGGAGGCTGCCATTCTGGAGCCTGGAAGCGTTTTTCTCATCGC
ATGCCCTGGCAGCGGAAAGACCCGCACGCTGACCTACAAGATCGCCTATGAGTTGTCCAGACTGAAATCGAACAAGCAGT
TCGTTGTTGCGATCACATATACGCACCGCGCGGCCGACGAAATCCATGAGCGCATTGAGGGCCTTGGAGTGGACACATCC
CGACTATGGATAGGCACGATCCACTCATTCTGTCTTGAATGGATACTGAAGCCCTACGGCATCTATCACGAGGCATTGGA
GCGGGGCTACCGCGTGATCGATCAGCATGAGCGCGAGAAGATTTTGGAGGCTCTGTGCAAGCCCTACCAGAAGCCAAAGA
TAACTTTCTGGGATTGCGAGTTTTATTTCACCGAAACTGGCTATGTGCTTTCCTGTCCACAAGCGTGGAAACACACCGGC
TTGCACGCCATATTGGGAAAGTATTTCGAGAAGCTGCAGGAAAGCCGGCAGATAGACTTTGAACTCATTCTCTACTATGC
GTATCAACTGATCGCCAGCCGTCCTGCGATCAGCGTCCTTCTAGGGCAGCTATTTTCTTTCGTGCTGATCGACGAGTATC
AGGACACCAAGCGAATTCAGTACTCGATCGTCACGGCCATCTTGAAAGCTGGCCAAGGTGCCACCAAGGCCTTCATCGTC
GGTGATCCGAATCAGGCAATTTACCAGTCGCTCGGGGGATACCCGATTGCCTTCGAGGATTTCAAGACGATGGCTGGCAT
TGACCTGAAGAAGCTCGAACTGTCGAAGAACTACCGTTCCTCTGAGCGCATCATCGACTATTTCGGAAATTACAACGTCC
ACGACACGTGCATTGAAGCTGCATCCGACGAAAAATCTTATCCGAGTCTTGTTTCGTTCGATGCCACGGTGAACAAGACA
GGCTTGGAAGCCGAGTTGATCCGGCTCATCCGTTTCAACATCGAGACTGTCGGTATCGCGCCTCACGAGGTGTGCGTGCT
TGCACCTCAGTGGACGCATCTTGCCAGCATGACACGCCGTCTGTGCGCGAGCATGCCCGAGTATTCGTTCGATGGCCCGG
GGATGGTTCCGTTTGCGCGTGACACTGAGAATTTTTGGTACAGGCTTTCCAAGATCGCACTTACCCAGGCATCGCCGGGA
ATGTATGTGCGCAGACTTCGTTGGGCCGGTGAAATCCTTACTGACCTGGAGGCGGCTGGCGCCAGCGTATCCAAGCTCAC
TCGAAAGTCGTTGTTGAGAGAATGCAATGCGATCAAGATCGATGAGACGGATGGACTCACCTACCTCAGCACATTCTTCG
AGAGGCTGTTTTCAAGTTTGGCCATCGATTTTCGACTCTTCACCACCCTTCGGGAGCATCACACTGCGTTCTTCGACAGT
TCGCAAGCGCGGGTTGCCCGATTGAAAAGTGAGGGAAGCGAATTCATCGGAGACATCGGAACCTTCAGGAAGGTTTTTCA
GAGCCGGACGGGCATCACTATCTCAACGATCCACGGCGTGAAGGGGGCAGAGTTCGATGTGGTGATCGCCTATGCCTTGC
TGGAGGACATGGTGCCGCACTTCAACGATCCGAACGGCCAAGAAAGTGCCATGAAACTCCTTTATGTCATTGGCTCGCGA
GCGAGGAAGAACTTGCACCTGATCTCGGAACGAGGTCGTATTCGCCAGTATTCTGGCGACGAGTACCAAGTGACGCGAAA
GCTCGCCGCATGTAGTTTTGGTTACGATCAAGTTCCATAG

Upstream 100 bases:

>100_bases
CCTTCAGAAAGGGAGATATCGATTTCGTTGGCATCAGGAACGAAATGCTGGCCACCTTCCCCGACGATCGTATCAACGAC
GTTCTCAAGGTCTTCTAGCC

Downstream 100 bases:

>100_bases
AACTTAGGATCGATGCTGCAGACGCCCGGTTACTGCGGGGGTTTCTTGGGCTGCTTCGAGGTGCGCGATTCCAGCAAGCG
CCGCGTAGCCTCCAGTTCCT

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MFTWDKEDLNPEQEAAILEPGSVFLIACPGSGKTRTLTYKIAYELSRLKSNKQFVVAITYTHRAADEIHERIEGLGVDTS
RLWIGTIHSFCLEWILKPYGIYHEALERGYRVIDQHEREKILEALCKPYQKPKITFWDCEFYFTETGYVLSCPQAWKHTG
LHAILGKYFEKLQESRQIDFELILYYAYQLIASRPAISVLLGQLFSFVLIDEYQDTKRIQYSIVTAILKAGQGATKAFIV
GDPNQAIYQSLGGYPIAFEDFKTMAGIDLKKLELSKNYRSSERIIDYFGNYNVHDTCIEAASDEKSYPSLVSFDATVNKT
GLEAELIRLIRFNIETVGIAPHEVCVLAPQWTHLASMTRRLCASMPEYSFDGPGMVPFARDTENFWYRLSKIALTQASPG
MYVRRLRWAGEILTDLEAAGASVSKLTRKSLLRECNAIKIDETDGLTYLSTFFERLFSSLAIDFRLFTTLREHHTAFFDS
SQARVARLKSEGSEFIGDIGTFRKVFQSRTGITISTIHGVKGAEFDVVIAYALLEDMVPHFNDPNGQESAMKLLYVIGSR
ARKNLHLISERGRIRQYSGDEYQVTRKLAACSFGYDQVP

Sequences:

>Translated_599_residues
MFTWDKEDLNPEQEAAILEPGSVFLIACPGSGKTRTLTYKIAYELSRLKSNKQFVVAITYTHRAADEIHERIEGLGVDTS
RLWIGTIHSFCLEWILKPYGIYHEALERGYRVIDQHEREKILEALCKPYQKPKITFWDCEFYFTETGYVLSCPQAWKHTG
LHAILGKYFEKLQESRQIDFELILYYAYQLIASRPAISVLLGQLFSFVLIDEYQDTKRIQYSIVTAILKAGQGATKAFIV
GDPNQAIYQSLGGYPIAFEDFKTMAGIDLKKLELSKNYRSSERIIDYFGNYNVHDTCIEAASDEKSYPSLVSFDATVNKT
GLEAELIRLIRFNIETVGIAPHEVCVLAPQWTHLASMTRRLCASMPEYSFDGPGMVPFARDTENFWYRLSKIALTQASPG
MYVRRLRWAGEILTDLEAAGASVSKLTRKSLLRECNAIKIDETDGLTYLSTFFERLFSSLAIDFRLFTTLREHHTAFFDS
SQARVARLKSEGSEFIGDIGTFRKVFQSRTGITISTIHGVKGAEFDVVIAYALLEDMVPHFNDPNGQESAMKLLYVIGSR
ARKNLHLISERGRIRQYSGDEYQVTRKLAACSFGYDQVP
>Mature_599_residues
MFTWDKEDLNPEQEAAILEPGSVFLIACPGSGKTRTLTYKIAYELSRLKSNKQFVVAITYTHRAADEIHERIEGLGVDTS
RLWIGTIHSFCLEWILKPYGIYHEALERGYRVIDQHEREKILEALCKPYQKPKITFWDCEFYFTETGYVLSCPQAWKHTG
LHAILGKYFEKLQESRQIDFELILYYAYQLIASRPAISVLLGQLFSFVLIDEYQDTKRIQYSIVTAILKAGQGATKAFIV
GDPNQAIYQSLGGYPIAFEDFKTMAGIDLKKLELSKNYRSSERIIDYFGNYNVHDTCIEAASDEKSYPSLVSFDATVNKT
GLEAELIRLIRFNIETVGIAPHEVCVLAPQWTHLASMTRRLCASMPEYSFDGPGMVPFARDTENFWYRLSKIALTQASPG
MYVRRLRWAGEILTDLEAAGASVSKLTRKSLLRECNAIKIDETDGLTYLSTFFERLFSSLAIDFRLFTTLREHHTAFFDS
SQARVARLKSEGSEFIGDIGTFRKVFQSRTGITISTIHGVKGAEFDVVIAYALLEDMVPHFNDPNGQESAMKLLYVIGSR
ARKNLHLISERGRIRQYSGDEYQVTRKLAACSFGYDQVP

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=295, Percent_Identity=28.135593220339, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=318, Percent_Identity=28.6163522012579, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 68178; Mature: 68178

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTWDKEDLNPEQEAAILEPGSVFLIACPGSGKTRTLTYKIAYELSRLKSNKQFVVAITY
CCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEEEHHHHHHHHCCCCCEEEEEEE
THRAADEIHERIEGLGVDTSRLWIGTIHSFCLEWILKPYGIYHEALERGYRVIDQHEREK
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEALCKPYQKPKITFWDCEFYFTETGYVLSCPQAWKHTGLHAILGKYFEKLQESRQIDF
HHHHHHCCCCCCCEEEEEEEEEEECCCCEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCH
ELILYYAYQLIASRPAISVLLGQLFSFVLIDEYQDTKRIQYSIVTAILKAGQGATKAFIV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
GDPNQAIYQSLGGYPIAFEDFKTMAGIDLKKLELSKNYRSSERIIDYFGNYNVHDTCIEA
ECCCHHHHHHCCCCCEEHHHHHHHHCCCHHEEHHHCCCCHHHHHHHHHCCCCCHHHHHHH
ASDEKSYPSLVSFDATVNKTGLEAELIRLIRFNIETVGIAPHEVCVLAPQWTHLASMTRR
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCEEECCCCCCEEEECCCHHHHHHHHHH
LCASMPEYSFDGPGMVPFARDTENFWYRLSKIALTQASPGMYVRRLRWAGEILTDLEAAG
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCC
ASVSKLTRKSLLRECNAIKIDETDGLTYLSTFFERLFSSLAIDFRLFTTLREHHTAFFDS
CHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SQARVARLKSEGSEFIGDIGTFRKVFQSRTGITISTIHGVKGAEFDVVIAYALLEDMVPH
CHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
FNDPNGQESAMKLLYVIGSRARKNLHLISERGRIRQYSGDEYQVTRKLAACSFGYDQVP
CCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFTWDKEDLNPEQEAAILEPGSVFLIACPGSGKTRTLTYKIAYELSRLKSNKQFVVAITY
CCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEEEEHHHHHHHHCCCCCEEEEEEE
THRAADEIHERIEGLGVDTSRLWIGTIHSFCLEWILKPYGIYHEALERGYRVIDQHEREK
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEALCKPYQKPKITFWDCEFYFTETGYVLSCPQAWKHTGLHAILGKYFEKLQESRQIDF
HHHHHHCCCCCCCEEEEEEEEEEECCCCEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCH
ELILYYAYQLIASRPAISVLLGQLFSFVLIDEYQDTKRIQYSIVTAILKAGQGATKAFIV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
GDPNQAIYQSLGGYPIAFEDFKTMAGIDLKKLELSKNYRSSERIIDYFGNYNVHDTCIEA
ECCCHHHHHHCCCCCEEHHHHHHHHCCCHHEEHHHCCCCHHHHHHHHHCCCCCHHHHHHH
ASDEKSYPSLVSFDATVNKTGLEAELIRLIRFNIETVGIAPHEVCVLAPQWTHLASMTRR
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCEEECCCCCCEEEECCCHHHHHHHHHH
LCASMPEYSFDGPGMVPFARDTENFWYRLSKIALTQASPGMYVRRLRWAGEILTDLEAAG
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCC
ASVSKLTRKSLLRECNAIKIDETDGLTYLSTFFERLFSSLAIDFRLFTTLREHHTAFFDS
CHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SQARVARLKSEGSEFIGDIGTFRKVFQSRTGITISTIHGVKGAEFDVVIAYALLEDMVPH
CHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
FNDPNGQESAMKLLYVIGSRARKNLHLISERGRIRQYSGDEYQVTRKLAACSFGYDQVP
CCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]