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The map label for this gene is gcvA [H]

Identifier: 120609347

GI number: 120609347

Start: 699035

End: 699940

Strand: Reverse

Name: gcvA [H]

Synonym: Aave_0647

Alternate gene names: 120609347

Gene position: 699940-699035 (Counterclockwise)

Preceding gene: 120609348

Following gene: 120609346

Centisome position: 13.08

GC content: 72.19

Gene sequence:

>906_bases
ATGGCCTCCGTCCACCCCGCTTCGCCGTCTTCCAAAAGCGCCGCCCAGCGCATCCCGCCGATCCAGTGCCTGCTGACCTT
CGAGGCGCTGGCGCGGTTGCGCAGCGTGACGCAGACGGCCGACGAGCTGTGCGTCACGCCCAGCGCGGTGAGCCACCGCG
TGCGCCAGCTGGAGCAGATCCTGGGCGCGCGGCTGTTCGGGCGGGCGGATTTCTCGCTCACCACCGAAGGCAGCGCCTAC
CTGGCCCACGTGCGCGAGGGCCTGGGCGCGCTGCAGCGCTTTCCGGGCACGGCGGCCGCGCCGGGCAAGCGGCGGCTCAA
GATCGCCGTCACGCCCACCTTCGCGCGGGCCATCCTGATCCCGCGCCTGCGCCAGTTCACCGAGGCCTACCCGGAGATCG
ACCTGGCGCTGCATGTCTCCATCCCCCTGCTCGACGTGGTCGCGGAGGATGCGGACCTCGTGGTGCGCTTCGGCCCCGGG
CACTACGCCGACATGGAGCACGTGGAGCTGTCGCGCGACGTCGTCACGCCCCTGGCCTCCCCCACCTTCCTGCGCGAACA
CGGCCCCTTCGAGCGCCCGGCGGACCTCGAAGGCACGCCCCTGCTGCGCAGCCCGCTGGAGCCGTGGCGCACCTGGTTCG
CCGCCGCCGGGCTCGGCTGGGCGGAGCCCTCGGAAGGCTCGCAGTTCAACGACATCGGCCTGATGTGCGACGCCGCATCG
GCCGGCATGGGCGTGGCGCTGGTGCGCCTCAAGCTCGGTGCGTCCTGGCTGGAGAACGGCACCCTCGTGCGGCTGTTCGC
GCTCGATGCGCCCAGCCCCCATGCCCACTACCTCTGCTGGCGCACCGGCACGATGGATCGCTGGGAATGCGCCGCCTTCG
CCGAATGGCTCCGGCGAGTCACTTGA

Upstream 100 bases:

>100_bases
ACACCGAGCACCGTGTGCTGCTCAACGGCCACAAGACGGTCATCTTCAAATAAAGTGGAACACCCCCCCGAGGCGCTGCG
CGCCTCGGGGAATCCATGTG

Downstream 100 bases:

>100_bases
CGTCACTCTCCTGAGGCCCTGCGGGCCTCCTGCATTGGTTCCAATGGCCCAGCCCCCCTCCCCCGCCGCCCCCTTCATCC
AGCGCCGCCCGAACGACGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MASVHPASPSSKSAAQRIPPIQCLLTFEALARLRSVTQTADELCVTPSAVSHRVRQLEQILGARLFGRADFSLTTEGSAY
LAHVREGLGALQRFPGTAAAPGKRRLKIAVTPTFARAILIPRLRQFTEAYPEIDLALHVSIPLLDVVAEDADLVVRFGPG
HYADMEHVELSRDVVTPLASPTFLREHGPFERPADLEGTPLLRSPLEPWRTWFAAAGLGWAEPSEGSQFNDIGLMCDAAS
AGMGVALVRLKLGASWLENGTLVRLFALDAPSPHAHYLCWRTGTMDRWECAAFAEWLRRVT

Sequences:

>Translated_301_residues
MASVHPASPSSKSAAQRIPPIQCLLTFEALARLRSVTQTADELCVTPSAVSHRVRQLEQILGARLFGRADFSLTTEGSAY
LAHVREGLGALQRFPGTAAAPGKRRLKIAVTPTFARAILIPRLRQFTEAYPEIDLALHVSIPLLDVVAEDADLVVRFGPG
HYADMEHVELSRDVVTPLASPTFLREHGPFERPADLEGTPLLRSPLEPWRTWFAAAGLGWAEPSEGSQFNDIGLMCDAAS
AGMGVALVRLKLGASWLENGTLVRLFALDAPSPHAHYLCWRTGTMDRWECAAFAEWLRRVT
>Mature_300_residues
ASVHPASPSSKSAAQRIPPIQCLLTFEALARLRSVTQTADELCVTPSAVSHRVRQLEQILGARLFGRADFSLTTEGSAYL
AHVREGLGALQRFPGTAAAPGKRRLKIAVTPTFARAILIPRLRQFTEAYPEIDLALHVSIPLLDVVAEDADLVVRFGPGH
YADMEHVELSRDVVTPLASPTFLREHGPFERPADLEGTPLLRSPLEPWRTWFAAAGLGWAEPSEGSQFNDIGLMCDAASA
GMGVALVRLKLGASWLENGTLVRLFALDAPSPHAHYLCWRTGTMDRWECAAFAEWLRRVT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=32.7526132404181, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=30.5263157894737, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788706, Length=285, Percent_Identity=29.8245614035088, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=28.673835125448, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=24.0677966101695, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787128, Length=251, Percent_Identity=28.6852589641434, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081978, Length=192, Percent_Identity=27.6041666666667, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32938; Mature: 32807

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVHPASPSSKSAAQRIPPIQCLLTFEALARLRSVTQTADELCVTPSAVSHRVRQLEQI
CCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGARLFGRADFSLTTEGSAYLAHVREGLGALQRFPGTAAAPGKRRLKIAVTPTFARAILI
HHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHH
PRLRQFTEAYPEIDLALHVSIPLLDVVAEDADLVVRFGPGHYADMEHVELSRDVVTPLAS
HHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
PTFLREHGPFERPADLEGTPLLRSPLEPWRTWFAAAGLGWAEPSEGSQFNDIGLMCDAAS
CHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCC
AGMGVALVRLKLGASWLENGTLVRLFALDAPSPHAHYLCWRTGTMDRWECAAFAEWLRRV
CCCHHHHHHHHHCHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHC
T
C
>Mature Secondary Structure 
ASVHPASPSSKSAAQRIPPIQCLLTFEALARLRSVTQTADELCVTPSAVSHRVRQLEQI
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGARLFGRADFSLTTEGSAYLAHVREGLGALQRFPGTAAAPGKRRLKIAVTPTFARAILI
HHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHH
PRLRQFTEAYPEIDLALHVSIPLLDVVAEDADLVVRFGPGHYADMEHVELSRDVVTPLAS
HHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
PTFLREHGPFERPADLEGTPLLRSPLEPWRTWFAAAGLGWAEPSEGSQFNDIGLMCDAAS
CHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCC
AGMGVALVRLKLGASWLENGTLVRLFALDAPSPHAHYLCWRTGTMDRWECAAFAEWLRRV
CCCHHHHHHHHHCHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]