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The map label for this gene is purU [H]

Identifier: 120609348

GI number: 120609348

Start: 699988

End: 700836

Strand: Reverse

Name: purU [H]

Synonym: Aave_0648

Alternate gene names: 120609348

Gene position: 700836-699988 (Counterclockwise)

Preceding gene: 120609352

Following gene: 120609347

Centisome position: 13.09

GC content: 65.72

Gene sequence:

>849_bases
ATGACCCAAGCCTATATTCTCACCCTCTCCTGCCCGGACAAGCTCGGGCTGGTGCATGCGGTCTCCGGATTCCTGCTCGA
GCATGGCGGCAACATCGAGGAAGCCGCCCAGTACAACGACCACGCCACCGGCCTGTTCTTCATGCGCGTGCGCTTCGCCT
GCGACACCCACGACCAGGCCGCGCTGCGCGCACGCCTCACCGAGTTCGCCGAGCCGCACCGCATGCACTGGAGCCTGCAC
GCCGCCAGCGAGCCCATGAAGACCGCCATCATGGTCAGCCGCGAAGGCCACTGCCTCAACGACCTGCTGTTCCGCTGGAA
ATCCGGGCTGCTGCCGGTGCACATCTGCGCCATCATCAGCAACCACCGCGACTTCTACCAGCTCGCGGCCAGCTACAACG
TGCCCTTCCACCACATCCCGGTCACGAAGGACAACAAGCCCCAGGCCGAGGCGCGCCAGTACGAGATCATCCAGCAGGAA
GGCGCCGAGCTGGTCGTGCTGGCCCGCTACATGCAGGTGCTGTCGGACGACCTGTGCCGCAAGCTGGAGGGCCGGGCCAT
CAACATCCACCACAGCTTCCTGCCCAGCTTCAAGGGCGCCAAGCCCTACTACCAGGCGCACGACCGCGGCGTGAAACTGA
TCGGTGCCACCGCCCACTACGTGACGGCCGACCTGGACGAAGGCCCGATCATCGAGCAGGACGTGGCCCGCGCCGACCAC
ACCGACACCGTGGAAGACCTGACGGCCCGCGGCCGCGACACCGAAAGCCAGGTACTGGCCCGCGCCGTGAAGTGGCACAC
CGAGCACCGTGTGCTGCTCAACGGCCACAAGACGGTCATCTTCAAATAA

Upstream 100 bases:

>100_bases
CCGTCCTCCCGGGAGCGTTGCGGGGGGCGCCCCCCTCCTCCTCATGCATCAATTCCTTGCTCCGCGTTGCTGCGGGCCGT
GCTGCGACAATCGCCCCGCT

Downstream 100 bases:

>100_bases
AGTGGAACACCCCCCCGAGGCGCTGCGCGCCTCGGGGAATCCATGTGATGGCCTCCGTCCACCCCGCTTCGCCGTCTTCC
AAAAGCGCCGCCCAGCGCAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTQAYILTLSCPDKLGLVHAVSGFLLEHGGNIEEAAQYNDHATGLFFMRVRFACDTHDQAALRARLTEFAEPHRMHWSLH
AASEPMKTAIMVSREGHCLNDLLFRWKSGLLPVHICAIISNHRDFYQLAASYNVPFHHIPVTKDNKPQAEARQYEIIQQE
GAELVVLARYMQVLSDDLCRKLEGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQDVARADH
TDTVEDLTARGRDTESQVLARAVKWHTEHRVLLNGHKTVIFK

Sequences:

>Translated_282_residues
MTQAYILTLSCPDKLGLVHAVSGFLLEHGGNIEEAAQYNDHATGLFFMRVRFACDTHDQAALRARLTEFAEPHRMHWSLH
AASEPMKTAIMVSREGHCLNDLLFRWKSGLLPVHICAIISNHRDFYQLAASYNVPFHHIPVTKDNKPQAEARQYEIIQQE
GAELVVLARYMQVLSDDLCRKLEGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQDVARADH
TDTVEDLTARGRDTESQVLARAVKWHTEHRVLLNGHKTVIFK
>Mature_281_residues
TQAYILTLSCPDKLGLVHAVSGFLLEHGGNIEEAAQYNDHATGLFFMRVRFACDTHDQAALRARLTEFAEPHRMHWSLHA
ASEPMKTAIMVSREGHCLNDLLFRWKSGLLPVHICAIISNHRDFYQLAASYNVPFHHIPVTKDNKPQAEARQYEIIQQEG
AELVVLARYMQVLSDDLCRKLEGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQDVARADHT
DTVEDLTARGRDTESQVLARAVKWHTEHRVLLNGHKTVIFK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI209869995, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI209869993, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=40.1433691756272, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=27.5132275132275, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=32.2222222222222, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31904; Mature: 31773

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAYILTLSCPDKLGLVHAVSGFLLEHGGNIEEAAQYNDHATGLFFMRVRFACDTHDQA
CCCEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCHHH
ALRARLTEFAEPHRMHWSLHAASEPMKTAIMVSREGHCLNDLLFRWKSGLLPVHICAIIS
HHHHHHHHHCCCHHEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHH
NHRDFYQLAASYNVPFHHIPVTKDNKPQAEARQYEIIQQEGAELVVLARYMQVLSDDLCR
CCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KLEGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQDVARADH
HHCCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEEEEECCCCCCCHHHHHCCCC
TDTVEDLTARGRDTESQVLARAVKWHTEHRVLLNGHKTVIFK
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC
>Mature Secondary Structure 
TQAYILTLSCPDKLGLVHAVSGFLLEHGGNIEEAAQYNDHATGLFFMRVRFACDTHDQA
CCEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCHHH
ALRARLTEFAEPHRMHWSLHAASEPMKTAIMVSREGHCLNDLLFRWKSGLLPVHICAIIS
HHHHHHHHHCCCHHEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHH
NHRDFYQLAASYNVPFHHIPVTKDNKPQAEARQYEIIQQEGAELVVLARYMQVLSDDLCR
CCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KLEGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQDVARADH
HHCCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEEEEECCCCCCCHHHHHCCCC
TDTVEDLTARGRDTESQVLARAVKWHTEHRVLLNGHKTVIFK
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]