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The map label for this gene is pilT [H]

Identifier: 120609337

GI number: 120609337

Start: 686971

End: 688014

Strand: Reverse

Name: pilT [H]

Synonym: Aave_0637

Alternate gene names: 120609337

Gene position: 688014-686971 (Counterclockwise)

Preceding gene: 120609339

Following gene: 120609336

Centisome position: 12.85

GC content: 65.42

Gene sequence:

>1044_bases
GTGGACATCACCCAACTGCTCGCCTTCAGCGTGAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGCCTGCCTCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAACGTCGATGCCCTCGACCACAAGACCGTGCACGCCATGGTGTACGACA
TCATGAGCGACGCCCAGCGCAAGGTGTACGAGGAGTTCCTGGAAGTGGACTTCTCCTTCGAGATCGAGGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGGACCATCCCGAGCAAGATCCTCACGCTCGA
GCAGCTCAACGCCCCGCGCATCTTCGCCGACCTTGCCCTCAAGCCGCGCGGCCTCGTGCTCGTCACCGGCCCCACCGGCT
CGGGCAAGTCCACCACGCTCGCGGCCATGGTGAACCACCTCAACGAGACCGAATACGGGCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCCTTCGCCGC
CGCGCTGAAATCCGCGCTGCGCGAAGATCCGGACGCCATCCTCGTGGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CCATGACCGCCGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACTTCCAGCGCGGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCCCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACGAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAGCAGCGTCGGCATGCAGACGCTGGACCAGAAC
CTCACCGACCTCGTGCGGCGCAACATCATCAGCCCCGCGGAAGCCCGCGGCAAGGCCAAGATTCCCGAGAACTTCCCGGG
CTGA

Upstream 100 bases:

>100_bases
GCGCAGGCTCGCGCGATGCGGTCCAGGACCCCTTGGAGGTTGTTACCAATCGTCGTCATAATGTTTGTTCTCAAGCGTAC
CAAACGACCAAGGGTTTTTC

Downstream 100 bases:

>100_bases
CACCGGCCCGCTCGCACCGCACCGTTCACCCATCCCCCAACACACCGCGCGGCAGACATCACCCCGCCGCGCGCACCGGA
GCACCTCCATGAAAGGCATC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSSSVGMQTLDQN
LTDLVRRNIISPAEARGKAKIPENFPG

Sequences:

>Translated_347_residues
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSSSVGMQTLDQN
LTDLVRRNIISPAEARGKAKIPENFPG
>Mature_347_residues
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSSSVGMQTLDQN
LTDLVRRNIISPAEARGKAKIPENFPG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=320, Percent_Identity=47.5, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789723, Length=347, Percent_Identity=31.9884726224784, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786296, Length=294, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38133; Mature: 38133

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KVYEEFLEVDFSFEIEGLARFRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLAL
HHHHHHHHCCCCEEECCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHCC
KPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVEDPIEFVHDSKKCLINQREVGP
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCHHHHCCCCCCCC
MTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QMYSTIQTSSSVGMQTLDQNLTDLVRRNIISPAEARGKAKIPENFPG
HHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHH
KVYEEFLEVDFSFEIEGLARFRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLAL
HHHHHHHHCCCCEEECCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHCC
KPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVEDPIEFVHDSKKCLINQREVGP
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCHHHHCCCCCCCC
MTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QMYSTIQTSSSVGMQTLDQNLTDLVRRNIISPAEARGKAKIPENFPG
HHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]