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The map label for this gene is garR [C]

Identifier: 120609333

GI number: 120609333

Start: 682865

End: 683803

Strand: Reverse

Name: garR [C]

Synonym: Aave_0633

Alternate gene names: 120609333

Gene position: 683803-682865 (Counterclockwise)

Preceding gene: 120609335

Following gene: 120609329

Centisome position: 12.77

GC content: 71.67

Gene sequence:

>939_bases
ATGCCCCCCAGCACCGCTCCGCGCAGCTACGACCCCACCCCCTCGCGCAAGGTCGCCTTCCTGGGACTGGGCGTGATGGG
CCACCCCATGGCGGGCCACCTCGCGCTCGCCGGCCACCAGGTCACCGTCTTCAACCGCACCGCCGCCAAGGCCGCGGCAT
GGTGCGAGGAGTTCGCTGCATCCGCCGGCGCACGGAAGGCAGCCACGCCGCGCGAAGCCGCGCAGGGGGCGGAGATCGTG
TTCTGCTGCGTGGGCAATGACGATGACCTGCGCTCGGTCGTGCTCGGGGCCGACGGTGCCTTCGCCGGCATGCAGCCCGG
CGCGGTCTTCGTGGACCACACCACCGCCTCCGCCGAAGTCGCGCGCGAACTGGCCCGGGCCGCACGCACCCTGGGCCTGC
GCTTCATCGACGCACCCGTCTCCGGCGGCCAGGCCGGCGCGCAGAACGGCCAGCTCACCGTCATGTGCGGCGGCGATGCG
GACGCCTTCGACGCCGTGCGGCCCGTCGCCATGGCATTCTCGCGCGCCTTCACGCGCATCGGCGACAGCGGCGCCGGGCA
GCTCGCCAAGATGGTCAACCAGATCTGCATCGCCGGCATCGTCCAGGGCCTCTCGGAAGCGGTCGCCTTCGGCCAGCATG
CCGGCCTGGACATGCCGCTGGTGCTGGACGTGATCGGCAAGGGCGCCGCCCAGAGCTGGCAGATGGACAACCGCGGCAAG
ACCATGGTCGAAGGCCGCTTCGACTTCGGCTTCGCCGTGGACTGGATGCGCAAGGACCTGGGCCTGGTGCTGGAAGAGGC
CCACCGCAACGGCGCGCGCCTGCCCCTCACCGCCCTCGTGGACCAGTTCTACGCCGACGTCCAGCAGGCCGGCGGCGGGC
GCTGGGACACGTCCAGCCTGATCACCCGCCTGGGCGTGAAAAAACCGCAGAACGGCTGA

Upstream 100 bases:

>100_bases
GCTGCGCGGCGGTGAGTCAACCCGTTCAGTGGCCAAGGCCGGCCGGCCTGCCTTGACGACTGAAGTTTGAACCAACTGAG
ACATATAAGGAGACATCCAC

Downstream 100 bases:

>100_bases
ATACGGCGCACGAAACCCGCACCGCCTCGCCCGTCGGAAGGCCCCGGAGCAGGCCACGCCAGCAGACGCCGCGGGACTGG
CTCTGCCAGGCCTCAGGGGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPPSTAPRSYDPTPSRKVAFLGLGVMGHPMAGHLALAGHQVTVFNRTAAKAAAWCEEFAASAGARKAATPREAAQGAEIV
FCCVGNDDDLRSVVLGADGAFAGMQPGAVFVDHTTASAEVARELARAARTLGLRFIDAPVSGGQAGAQNGQLTVMCGGDA
DAFDAVRPVAMAFSRAFTRIGDSGAGQLAKMVNQICIAGIVQGLSEAVAFGQHAGLDMPLVLDVIGKGAAQSWQMDNRGK
TMVEGRFDFGFAVDWMRKDLGLVLEEAHRNGARLPLTALVDQFYADVQQAGGGRWDTSSLITRLGVKKPQNG

Sequences:

>Translated_312_residues
MPPSTAPRSYDPTPSRKVAFLGLGVMGHPMAGHLALAGHQVTVFNRTAAKAAAWCEEFAASAGARKAATPREAAQGAEIV
FCCVGNDDDLRSVVLGADGAFAGMQPGAVFVDHTTASAEVARELARAARTLGLRFIDAPVSGGQAGAQNGQLTVMCGGDA
DAFDAVRPVAMAFSRAFTRIGDSGAGQLAKMVNQICIAGIVQGLSEAVAFGQHAGLDMPLVLDVIGKGAAQSWQMDNRGK
TMVEGRFDFGFAVDWMRKDLGLVLEEAHRNGARLPLTALVDQFYADVQQAGGGRWDTSSLITRLGVKKPQNG
>Mature_311_residues
PPSTAPRSYDPTPSRKVAFLGLGVMGHPMAGHLALAGHQVTVFNRTAAKAAAWCEEFAASAGARKAATPREAAQGAEIVF
CCVGNDDDLRSVVLGADGAFAGMQPGAVFVDHTTASAEVARELARAARTLGLRFIDAPVSGGQAGAQNGQLTVMCGGDAD
AFDAVRPVAMAFSRAFTRIGDSGAGQLAKMVNQICIAGIVQGLSEAVAFGQHAGLDMPLVLDVIGKGAAQSWQMDNRGKT
MVEGRFDFGFAVDWMRKDLGLVLEEAHRNGARLPLTALVDQFYADVQQAGGGRWDTSSLITRLGVKKPQNG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=31.3167259786477, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=29.2517006802721, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=33.8028169014084, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1786719, Length=288, Percent_Identity=33.6805555555556, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790315, Length=296, Percent_Identity=28.7162162162162, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=33.5664335664336, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=28.9377289377289, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574115, Length=295, Percent_Identity=25.4237288135593, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=31.0580204778157, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=31.0580204778157, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 32557; Mature: 32426

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSTAPRSYDPTPSRKVAFLGLGVMGHPMAGHLALAGHQVTVFNRTAAKAAAWCEEFAA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCEEEEEECHHHHHHHHHHHHHH
SAGARKAATPREAAQGAEIVFCCVGNDDDLRSVVLGADGAFAGMQPGAVFVDHTTASAEV
HCCCCCCCCCHHHHCCCCEEEEEECCCCCHHEEEEECCCCCCCCCCCEEEEECCCHHHHH
ARELARAARTLGLRFIDAPVSGGQAGAQNGQLTVMCGGDADAFDAVRPVAMAFSRAFTRI
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHC
GDSGAGQLAKMVNQICIAGIVQGLSEAVAFGQHAGLDMPLVLDVIGKGAAQSWQMDNRGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCC
TMVEGRFDFGFAVDWMRKDLGLVLEEAHRNGARLPLTALVDQFYADVQQAGGGRWDTSSL
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
ITRLGVKKPQNG
HHHHCCCCCCCC
>Mature Secondary Structure 
PPSTAPRSYDPTPSRKVAFLGLGVMGHPMAGHLALAGHQVTVFNRTAAKAAAWCEEFAA
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCEEEEEECHHHHHHHHHHHHHH
SAGARKAATPREAAQGAEIVFCCVGNDDDLRSVVLGADGAFAGMQPGAVFVDHTTASAEV
HCCCCCCCCCHHHHCCCCEEEEEECCCCCHHEEEEECCCCCCCCCCCEEEEECCCHHHHH
ARELARAARTLGLRFIDAPVSGGQAGAQNGQLTVMCGGDADAFDAVRPVAMAFSRAFTRI
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHC
GDSGAGQLAKMVNQICIAGIVQGLSEAVAFGQHAGLDMPLVLDVIGKGAAQSWQMDNRGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCC
TMVEGRFDFGFAVDWMRKDLGLVLEEAHRNGARLPLTALVDQFYADVQQAGGGRWDTSSL
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
ITRLGVKKPQNG
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA