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The map label for this gene is pilT [H]

Identifier: 120609335

GI number: 120609335

Start: 685079

End: 686215

Strand: Reverse

Name: pilT [H]

Synonym: Aave_0635

Alternate gene names: 120609335

Gene position: 686215-685079 (Counterclockwise)

Preceding gene: 120609336

Following gene: 120609333

Centisome position: 12.82

GC content: 65.17

Gene sequence:

>1137_bases
ATGGAACGCGATCAGGCCAGCAAATTCATCAACGACCTGCTGAAGCTCATGGTGGGGCGCGGCGGAAGCGATCTTTTCAT
CACGGCGGAGTTCCCTCCCGCCATCAAGGTGGACGGAAAGGTCACGAAGGTATCCCCCCAGCCGCTCACGCCGGCGCACA
CCCTCACGCTCGCGCGCGCCATCATGAGCGACAAGCAGGTGGCGGACTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCCCCCGCGGGCATCGGCCGCTTCCGGGTGAACGCCTTCATCCAGCAGGGCCGCGTCGGCATGGTGCTGCGGACCATCCC
GCTCACGCTGCCCACCATCGACGGGCTGGGCGTGCCGCAGGTCCTCAAGGAAATCACCATGTCCAAGCGCGGGCTGTGCA
TCCTCGTGGGTGCCACCGGCTCGGGCAAGTCCACCACCCTCGCGGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACCGTGGAAGACCCGATCGAGTTCGTGCACCCGCACAAGAACTGCGTCGTCACCCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCCGCCCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTCATGGGCGAGATCCGCG
ACCGCGAAACCATGGACCATGCCATCGCGTTCTCCGAGACCGGCCACCTGTGCCTCGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAACGGCGCGCGCAACTGCTCATGGACCTGTCGCTCAACCT
GCGCGCCATGGTGTCGCAGCGCCTCATCCCCAAGCAGGACGGCAAGGGCCGCGCCGCGGCGGTGGAAGTGATGCTCAACA
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTTCGAGGCCAACGTCATCAGCTACGAAGACGCGCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGGCTGCAGATCAAGCTCGGCAGCCAGCGCGCCCGCAGCACCGACCTCTCATCGGGTACCGAGC
ACTTCGCCATCGTCTGA

Upstream 100 bases:

>100_bases
GCCCTGCTCGCGCGCAAGCTATCGCAGCGCCTGCGGGTCGTCAGCGCCCGCCTCAGCGACAGCCAGCGCTGACGCGGCGG
CGCACCGACCGGAGCACTGA

Downstream 100 bases:

>100_bases
GGCCAACCCGCGCCGCGCCCGCCGCCATCCGGCGGGCGCTGTCTTTTTCATCGTTTTCCGGAACCACGACAGCAACCATC
TTGAATGACTAGCTCAAAGA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVISYEDALRNADSLNDLRLQIKLGSQRARSTDLSSGTEHFAIV

Sequences:

>Translated_378_residues
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVISYEDALRNADSLNDLRLQIKLGSQRARSTDLSSGTEHFAIV
>Mature_378_residues
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVISYEDALRNADSLNDLRLQIKLGSQRARSTDLSSGTEHFAIV

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=37.6175548589342, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=39.2405063291139, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786296, Length=333, Percent_Identity=27.6276276276276, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41749; Mature: 41749

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARA
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCEEEEECCCCCCCHHHHHHHHH
IMSDKQVADFERTKECNFAISPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQ
HHCCCHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEEECCEECCCCCCCHHH
VLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFGHIITVEDPIEFVHPHKNCVV
HHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHCCCCCEEE
TQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
EHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHEEECCCCCEEEEEEECCCH
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADL
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHH
IFKGEVAEIKEIMKKSRNLGMQTFDQSLFDLFEANVISYEDALRNADSLNDLRLQIKLGS
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEEECC
QRARSTDLSSGTEHFAIV
CCCCCCCCCCCCCCEEEC
>Mature Secondary Structure
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARA
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCEEEEECCCCCCCHHHHHHHHH
IMSDKQVADFERTKECNFAISPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQ
HHCCCHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEEECCEECCCCCCCHHH
VLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFGHIITVEDPIEFVHPHKNCVV
HHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHCCCCCEEE
TQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
EHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHEEECCCCCEEEEEEECCCH
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADL
HHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHH
IFKGEVAEIKEIMKKSRNLGMQTFDQSLFDLFEANVISYEDALRNADSLNDLRLQIKLGS
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEEECC
QRARSTDLSSGTEHFAIV
CCCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]