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The map label for this gene is yafJ [C]

Identifier: 120609106

GI number: 120609106

Start: 433416

End: 434183

Strand: Reverse

Name: yafJ [C]

Synonym: Aave_0403

Alternate gene names: 120609106

Gene position: 434183-433416 (Counterclockwise)

Preceding gene: 120609107

Following gene: 120609105

Centisome position: 8.11

GC content: 66.8

Gene sequence:

>768_bases
ATGTGCCAGTTGCTCGGAATGAACGCCAACACGCCCACCGACGTGACCTTCAGCTTCACGGGGTTCGCGCAGCGCGGCGG
CAGGACGGGCGACCACTCCGACGGCTGGGGCATCGCCTTCTTCGAGGACAAGGGCCTGCGGCATTTCGTGGACCACGAAC
GGGCCGCGGATTCGCCGATCGCGGAACTGATACGCCGCTATCCCATCCGCAGCCGCAACGTCATCGCGCACATCCGCAAG
GCCACCCAGGGCAGCGTGGCCCTGCAGAACTGCCACCCGTTCGTGCGCGAACTCTGGGGCCGCTACTGGGTGTTCGCGCA
CAACGGCGACCTGAAGGACTTTCGTCCCCGCCTGCATTCGCACTTCCATCCCGTCGGCGCCACCGACAGCGAGCACGCGT
TCTGCTGGATCATGCAGGAGCTCTCCAAATCCCATGCCGACGTGCCCAGCGTGCAGGAACTCACCCTCACCCTGCGAGAA
CTGGCGGCGCGCATCGCGCCGCATGGCACCTTCAACTTCCTGCTGTCCAACGGGCAGGCCCTCTGGGCCCATGCATCCAC
CCACCTCTGCTACATCGAGCGCCGGCATCCGTTCTCGCAGGCCTGCCTGTGCGACGAGGACCTGAGCGTGGACTTCTCGG
CCCACACCACGCCGCACGACCGCGTGGCGGTGGTGGTGACGGCGCCCCTGACCAGCGACGAAGTCTGGACGCCGTTCCGC
AGCGGCGAACTCAAGGTGTTCGTGGACGGCGCCTGCCAGGCCTTCTGA

Upstream 100 bases:

>100_bases
CGCGCGAAGGCTGACCGCCGCCACGGCGAAGGCCCCCGCTCCGCGGGCCCCGCCGCCAGGCAGCAGCCCCTCCGATATTT
CTCTGCCTCCTCCATCGACC

Downstream 100 bases:

>100_bases
CCCTGGGCAGGCGGCGCCCCGCGCGCTTCAGCCCAGCGGCTTCCACACCGGCGGTTCGGGATCGGGAAAGCAGGGCGCGT
GGCCTCCCGGCCGCAGCATG

Product: class-II glutamine amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCQLLGMNANTPTDVTFSFTGFAQRGGRTGDHSDGWGIAFFEDKGLRHFVDHERAADSPIAELIRRYPIRSRNVIAHIRK
ATQGSVALQNCHPFVRELWGRYWVFAHNGDLKDFRPRLHSHFHPVGATDSEHAFCWIMQELSKSHADVPSVQELTLTLRE
LAARIAPHGTFNFLLSNGQALWAHASTHLCYIERRHPFSQACLCDEDLSVDFSAHTTPHDRVAVVVTAPLTSDEVWTPFR
SGELKVFVDGACQAF

Sequences:

>Translated_255_residues
MCQLLGMNANTPTDVTFSFTGFAQRGGRTGDHSDGWGIAFFEDKGLRHFVDHERAADSPIAELIRRYPIRSRNVIAHIRK
ATQGSVALQNCHPFVRELWGRYWVFAHNGDLKDFRPRLHSHFHPVGATDSEHAFCWIMQELSKSHADVPSVQELTLTLRE
LAARIAPHGTFNFLLSNGQALWAHASTHLCYIERRHPFSQACLCDEDLSVDFSAHTTPHDRVAVVVTAPLTSDEVWTPFR
SGELKVFVDGACQAF
>Mature_255_residues
MCQLLGMNANTPTDVTFSFTGFAQRGGRTGDHSDGWGIAFFEDKGLRHFVDHERAADSPIAELIRRYPIRSRNVIAHIRK
ATQGSVALQNCHPFVRELWGRYWVFAHNGDLKDFRPRLHSHFHPVGATDSEHAFCWIMQELSKSHADVPSVQELTLTLRE
LAARIAPHGTFNFLLSNGQALWAHASTHLCYIERRHPFSQACLCDEDLSVDFSAHTTPHDRVAVVVTAPLTSDEVWTPFR
SGELKVFVDGACQAF

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=251, Percent_Identity=49.800796812749, Blast_Score=269, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 28706; Mature: 28706

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNANTPTDVTFSFTGFAQRGGRTGDHSDGWGIAFFEDKGLRHFVDHERAADSPI
CCEEEECCCCCCCCEEEEEECHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCHH
AELIRRYPIRSRNVIAHIRKATQGSVALQNCHPFVRELWGRYWVFAHNGDLKDFRPRLHS
HHHHHHCCCCCCHHHHHHHHHCCCCEEHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHH
HFHPVGATDSEHAFCWIMQELSKSHADVPSVQELTLTLRELAARIAPHGTFNFLLSNGQA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LWAHASTHLCYIERRHPFSQACLCDEDLSVDFSAHTTPHDRVAVVVTAPLTSDEVWTPFR
EEEECCCEEEEEECCCCCHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCC
SGELKVFVDGACQAF
CCCEEEEECCCCCCC
>Mature Secondary Structure
MCQLLGMNANTPTDVTFSFTGFAQRGGRTGDHSDGWGIAFFEDKGLRHFVDHERAADSPI
CCEEEECCCCCCCCEEEEEECHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCHH
AELIRRYPIRSRNVIAHIRKATQGSVALQNCHPFVRELWGRYWVFAHNGDLKDFRPRLHS
HHHHHHCCCCCCHHHHHHHHHCCCCEEHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHH
HFHPVGATDSEHAFCWIMQELSKSHADVPSVQELTLTLRELAARIAPHGTFNFLLSNGQA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
LWAHASTHLCYIERRHPFSQACLCDEDLSVDFSAHTTPHDRVAVVVTAPLTSDEVWTPFR
EEEECCCEEEEEECCCCCHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCC
SGELKVFVDGACQAF
CCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]