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The map label for this gene is yhcA [H]

Identifier: 120609107

GI number: 120609107

Start: 434270

End: 435718

Strand: Reverse

Name: yhcA [H]

Synonym: Aave_0404

Alternate gene names: 120609107

Gene position: 435718-434270 (Counterclockwise)

Preceding gene: 120609108

Following gene: 120609106

Centisome position: 8.14

GC content: 70.74

Gene sequence:

>1449_bases
ATGACGGGTTCCGCGGCGCTGCTGCGCGAGCGCTACGGCGAACGCTACCGGTGGCTGCTGCTGCTCTCGGTCATGGTCGG
CACCATGGCGTCGATCATGTCCTCCACCATCGTGAACGTGGCGATCCCGGACATGAGCCACCACTTCACGCTGGGGCAGG
CGCGGGCCCAGTGGGTCACCTCGGGCTTCATGGTGGCCACCACCGTGGCCATGCTCACCACGCCCTGGCTGCTGGCGCGC
TACGGCTACCGGGCCACCTACGTGGGCGCCATGGTGCTGCTGCTGGTGGGCGGCATCGGCGGCGGCGTCGCCGGCCAGTA
CGAATGGGTGCTGCTGGCCCGCGTGCTCGAGGGCCTGGCGGCCGGGGTGGTGCAGCCCATCCCGGCGGTGATCATCCTGC
ATGCGTTCCAGCCGCACGAGCAGGGCCGCGCCAGCGGCATCTTCGGCATGGGCGTGGTGCTGGCGCCCGCCATCGGCCCG
AGCGTCGGCGGCCTGCTCGTGGACTGGTTCGGCTGGCGCTCCATCTTCTTCATGGTGGTGCCGTTCTGCATCGCCTCCCT
CGCCCTCGCGTACAGGTACGTGCCGGTCAGCAGTCCGGGCGGCGTGGCCGCGGACCGCTCCGGCGCCTCGCTCGACTCGG
CCGGCCTGCTGCTCGGCGCGGTGGGCACGCTGTGCCTGCTCAACGGGCTGGTATCGCTGCGCGACGGCCCCGCATTCACG
TCGGCCGCCCTGCTCCTGTCCGCGCTCGCAGCCCTGGCCGCGTTCATCGCCTGGGAACGCCGCATGCTGGCACGCCAGGG
CAGGCCGCTGATGGACCTGCGGCTGTTCGAGGTGCGGCAGTTCGCCATGGGCAGCATCGTGGCGTTCATCTACGGCACCG
CGCTTTTCGGGTCCACCTACCTGCTGCCGGTGTTCCTGCAACTGGGCCTGCAAATGTCGGCATCGCACGTCGGCACGCTG
ATGCTGCCGGCGGGCATCGTGCTGGCGATCACCATTCCCCTGGTCGGGCGCATGGCCGACCGCCAGCCCACGCACCTGCT
CGTGGGCACCGGACTGGCTTTGCTGGCGCTGTCCTTCGGCCTGATGGCGTTCGTGGAACTGCACACCGCCCTGTGGCTGC
TGGTGGCGTTCGCGGTGCTGGGCCGCATCGGCCTGGGCTTCATCCTGCCGTCCCTCAACCTGGGCGCGATGCGGCCGCTG
CCCAAGCCGCTCATCGCGCAGGGCTCGAGCGCCATCAGCTTCGTGCGCATGCTCGGCGGCGCGGCCGGGGTGAGCCTGTG
CGGCATCGTGCTCGAATGGCGCATCGCCGCGCACGGCGATTCGCTGCAGAATGCGGTCAGCAGCCCGGAACGCATCGCCG
CCTTCGGTGATACCTTCCTCCTGCTCACCGGGCTGTGCCTGCTGGCCCTGGTGGCAGCCTGGCAGTTGCGCCCGCCGCGC
GAAGGCTGA

Upstream 100 bases:

>100_bases
GCGGCTGCGCGGCGGGGCGGCCCTGGCGCGGCGTCTGCTGGCAGGGGCCGCGCCTGCCGCACAGCGTGGTGCGCCGGCCA
GCCCCGTTCCGGGGCATGGC

Downstream 100 bases:

>100_bases
CCGCCGCCACGGCGAAGGCCCCCGCTCCGCGGGCCCCGCCGCCAGGCAGCAGCCCCTCCGATATTTCTCTGCCTCCTCCA
TCGACCATGTGCCAGTTGCT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTGSAALLRERYGERYRWLLLLSVMVGTMASIMSSTIVNVAIPDMSHHFTLGQARAQWVTSGFMVATTVAMLTTPWLLAR
YGYRATYVGAMVLLLVGGIGGGVAGQYEWVLLARVLEGLAAGVVQPIPAVIILHAFQPHEQGRASGIFGMGVVLAPAIGP
SVGGLLVDWFGWRSIFFMVVPFCIASLALAYRYVPVSSPGGVAADRSGASLDSAGLLLGAVGTLCLLNGLVSLRDGPAFT
SAALLLSALAALAAFIAWERRMLARQGRPLMDLRLFEVRQFAMGSIVAFIYGTALFGSTYLLPVFLQLGLQMSASHVGTL
MLPAGIVLAITIPLVGRMADRQPTHLLVGTGLALLALSFGLMAFVELHTALWLLVAFAVLGRIGLGFILPSLNLGAMRPL
PKPLIAQGSSAISFVRMLGGAAGVSLCGIVLEWRIAAHGDSLQNAVSSPERIAAFGDTFLLLTGLCLLALVAAWQLRPPR
EG

Sequences:

>Translated_482_residues
MTGSAALLRERYGERYRWLLLLSVMVGTMASIMSSTIVNVAIPDMSHHFTLGQARAQWVTSGFMVATTVAMLTTPWLLAR
YGYRATYVGAMVLLLVGGIGGGVAGQYEWVLLARVLEGLAAGVVQPIPAVIILHAFQPHEQGRASGIFGMGVVLAPAIGP
SVGGLLVDWFGWRSIFFMVVPFCIASLALAYRYVPVSSPGGVAADRSGASLDSAGLLLGAVGTLCLLNGLVSLRDGPAFT
SAALLLSALAALAAFIAWERRMLARQGRPLMDLRLFEVRQFAMGSIVAFIYGTALFGSTYLLPVFLQLGLQMSASHVGTL
MLPAGIVLAITIPLVGRMADRQPTHLLVGTGLALLALSFGLMAFVELHTALWLLVAFAVLGRIGLGFILPSLNLGAMRPL
PKPLIAQGSSAISFVRMLGGAAGVSLCGIVLEWRIAAHGDSLQNAVSSPERIAAFGDTFLLLTGLCLLALVAAWQLRPPR
EG
>Mature_481_residues
TGSAALLRERYGERYRWLLLLSVMVGTMASIMSSTIVNVAIPDMSHHFTLGQARAQWVTSGFMVATTVAMLTTPWLLARY
GYRATYVGAMVLLLVGGIGGGVAGQYEWVLLARVLEGLAAGVVQPIPAVIILHAFQPHEQGRASGIFGMGVVLAPAIGPS
VGGLLVDWFGWRSIFFMVVPFCIASLALAYRYVPVSSPGGVAADRSGASLDSAGLLLGAVGTLCLLNGLVSLRDGPAFTS
AALLLSALAALAAFIAWERRMLARQGRPLMDLRLFEVRQFAMGSIVAFIYGTALFGSTYLLPVFLQLGLQMSASHVGTLM
LPAGIVLAITIPLVGRMADRQPTHLLVGTGLALLALSFGLMAFVELHTALWLLVAFAVLGRIGLGFILPSLNLGAMRPLP
KPLIAQGSSAISFVRMLGGAAGVSLCGIVLEWRIAAHGDSLQNAVSSPERIAAFGDTFLLLTGLCLLALVAAWQLRPPRE
G

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=439, Percent_Identity=25.7403189066059, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788710, Length=437, Percent_Identity=26.0869565217391, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=23.5294117647059, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790195, Length=405, Percent_Identity=23.7037037037037, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788392, Length=438, Percent_Identity=27.8538812785388, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50836; Mature: 50705

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSAALLRERYGERYRWLLLLSVMVGTMASIMSSTIVNVAIPDMSHHFTLGQARAQWVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEHHHHHHHHHH
SGFMVATTVAMLTTPWLLARYGYRATYVGAMVLLLVGGIGGGVAGQYEWVLLARVLEGLA
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AGVVQPIPAVIILHAFQPHEQGRASGIFGMGVVLAPAIGPSVGGLLVDWFGWRSIFFMVV
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
PFCIASLALAYRYVPVSSPGGVAADRSGASLDSAGLLLGAVGTLCLLNGLVSLRDGPAFT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SAALLLSALAALAAFIAWERRMLARQGRPLMDLRLFEVRQFAMGSIVAFIYGTALFGSTY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPVFLQLGLQMSASHVGTLMLPAGIVLAITIPLVGRMADRQPTHLLVGTGLALLALSFG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
LMAFVELHTALWLLVAFAVLGRIGLGFILPSLNLGAMRPLPKPLIAQGSSAISFVRMLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCC
AAGVSLCGIVLEWRIAAHGDSLQNAVSSPERIAAFGDTFLLLTGLCLLALVAAWQLRPPR
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCC
EG
CC
>Mature Secondary Structure 
TGSAALLRERYGERYRWLLLLSVMVGTMASIMSSTIVNVAIPDMSHHFTLGQARAQWVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEHHHHHHHHHH
SGFMVATTVAMLTTPWLLARYGYRATYVGAMVLLLVGGIGGGVAGQYEWVLLARVLEGLA
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AGVVQPIPAVIILHAFQPHEQGRASGIFGMGVVLAPAIGPSVGGLLVDWFGWRSIFFMVV
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
PFCIASLALAYRYVPVSSPGGVAADRSGASLDSAGLLLGAVGTLCLLNGLVSLRDGPAFT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SAALLLSALAALAAFIAWERRMLARQGRPLMDLRLFEVRQFAMGSIVAFIYGTALFGSTY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPVFLQLGLQMSASHVGTLMLPAGIVLAITIPLVGRMADRQPTHLLVGTGLALLALSFG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
LMAFVELHTALWLLVAFAVLGRIGLGFILPSLNLGAMRPLPKPLIAQGSSAISFVRMLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCC
AAGVSLCGIVLEWRIAAHGDSLQNAVSSPERIAAFGDTFLLLTGLCLLALVAAWQLRPPR
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCC
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]