Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 120609066

GI number: 120609066

Start: 391008

End: 391703

Strand: Reverse

Name: lipB

Synonym: Aave_0363

Alternate gene names: 120609066

Gene position: 391703-391008 (Counterclockwise)

Preceding gene: 120609067

Following gene: 120609065

Centisome position: 7.32

GC content: 70.26

Gene sequence:

>696_bases
ATGCAGGTGCGGATGCTCGGGCGGGTGGACTTGCGCGAAACGGTGGAGGCCATGCAGGCGTTCACCGCGCAGCGCACCGG
CGACACGCCCGATGTCCTGTGGGTGTGCGAGCACGCCCCCCATTTCACGCAGGGCCTCGCGGGCCACGCGGACCACCTGC
TGGCCCCTGGCGACATCCCCGTGGTCGCCACCAACCGCGGTGGGCAGGTCACCTTCCACGGCCCCGGGCAGGTGGTGGCC
TATCCGCTCGTCGATCTGCGGCGTGCCGGCTACTACGTGAAGGAATACGTCCACCGCGTGGAAGAGGCCGCGATCCGCAC
GCTCGCGCATTTCGGCGTCACGGGCCACCGCGTGGCCGGCGCGCCGGGCATCTACGTGCGGCTGGACGATCCGCGCAGCC
ACGCCCTGCTGCCCCAGCGCCCGCGGAAGGCCGACCCGCTGGAGCCCGCGGCGGCCGTGCCCGACTTCACCGGGCTGGGC
AAGATCGCCGCGCTCGGCATCAAGGTTTCGCGCCACTGCACCTACCACGGCGTGGCACTCAACGTGGACATGGACCTGGA
ACCCTTCTCGCGCATCAACCCTTGCGGCTACGCAGGGTTGCCGACCGTGGACCTTTCTACAATCGGCGTGCACACCACCT
GGGACGAAGCGGCTTCCGTGCTGGCCAGCCAATTGGCCATCCGCCTCGCGCCCTGA

Upstream 100 bases:

>100_bases
TCACCGTGACGGCGACCAGCCGCGAACAGCTCGACGACCTGTACCGCGCGCTTTCCTCGCACCCGATGGTGAAGGTGGTG
CTCTGATCCGGGACGCGGCG

Downstream 100 bases:

>100_bases
CCTCAGCACCAGCCATGAGCACTCCCGAAGTCGTGCGCGAAGCGCAATCCACCGTCGCCTACAACCCGCTCGCCAAGCAG
AAGGCCGCCGCGAAGCTTTC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQVRMLGRVDLRETVEAMQAFTAQRTGDTPDVLWVCEHAPHFTQGLAGHADHLLAPGDIPVVATNRGGQVTFHGPGQVVA
YPLVDLRRAGYYVKEYVHRVEEAAIRTLAHFGVTGHRVAGAPGIYVRLDDPRSHALLPQRPRKADPLEPAAAVPDFTGLG
KIAALGIKVSRHCTYHGVALNVDMDLEPFSRINPCGYAGLPTVDLSTIGVHTTWDEAASVLASQLAIRLAP

Sequences:

>Translated_231_residues
MQVRMLGRVDLRETVEAMQAFTAQRTGDTPDVLWVCEHAPHFTQGLAGHADHLLAPGDIPVVATNRGGQVTFHGPGQVVA
YPLVDLRRAGYYVKEYVHRVEEAAIRTLAHFGVTGHRVAGAPGIYVRLDDPRSHALLPQRPRKADPLEPAAAVPDFTGLG
KIAALGIKVSRHCTYHGVALNVDMDLEPFSRINPCGYAGLPTVDLSTIGVHTTWDEAASVLASQLAIRLAP
>Mature_231_residues
MQVRMLGRVDLRETVEAMQAFTAQRTGDTPDVLWVCEHAPHFTQGLAGHADHLLAPGDIPVVATNRGGQVTFHGPGQVVA
YPLVDLRRAGYYVKEYVHRVEEAAIRTLAHFGVTGHRVAGAPGIYVRLDDPRSHALLPQRPRKADPLEPAAAVPDFTGLG
KIAALGIKVSRHCTYHGVALNVDMDLEPFSRINPCGYAGLPTVDLSTIGVHTTWDEAASVLASQLAIRLAP

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=230, Percent_Identity=41.7391304347826, Blast_Score=175, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_ACIAC (A1TJ32)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_968744.1
- ProteinModelPortal:   A1TJ32
- SMR:   A1TJ32
- STRING:   A1TJ32
- GeneID:   4665327
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_0363
- NMPDR:   fig|397945.5.peg.327
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   A1TJ32
- ProtClustDB:   PRK14346
- BioCyc:   AAVE397945:AAVE_0363-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24928; Mature: 24928

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVRMLGRVDLRETVEAMQAFTAQRTGDTPDVLWVCEHAPHFTQGLAGHADHLLAPGDIP
CCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCCCCCCEECCCCCE
VVATNRGGQVTFHGPGQVVAYPLVDLRRAGYYVKEYVHRVEEAAIRTLAHFGVTGHRVAG
EEEECCCCEEEECCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
APGIYVRLDDPRSHALLPQRPRKADPLEPAAAVPDFTGLGKIAALGIKVSRHCTYHGVAL
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEECCEEEEEEEE
NVDMDLEPFSRINPCGYAGLPTVDLSTIGVHTTWDEAASVLASQLAIRLAP
EECCCCCCHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MQVRMLGRVDLRETVEAMQAFTAQRTGDTPDVLWVCEHAPHFTQGLAGHADHLLAPGDIP
CCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCCCCCCEECCCCCE
VVATNRGGQVTFHGPGQVVAYPLVDLRRAGYYVKEYVHRVEEAAIRTLAHFGVTGHRVAG
EEEECCCCEEEECCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
APGIYVRLDDPRSHALLPQRPRKADPLEPAAAVPDFTGLGKIAALGIKVSRHCTYHGVAL
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEEECCEEEEEEEE
NVDMDLEPFSRINPCGYAGLPTVDLSTIGVHTTWDEAASVLASQLAIRLAP
EECCCCCCHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA