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The map label for this gene is lipA

Identifier: 120609065

GI number: 120609065

Start: 389995

End: 390993

Strand: Reverse

Name: lipA

Synonym: Aave_0362

Alternate gene names: 120609065

Gene position: 390993-389995 (Counterclockwise)

Preceding gene: 120609066

Following gene: 120609053

Centisome position: 7.3

GC content: 66.57

Gene sequence:

>999_bases
ATGAGCACTCCCGAAGTCGTGCGCGAAGCGCAATCCACCGTCGCCTACAACCCGCTCGCCAAGCAGAAGGCCGCCGCGAA
GCTTTCCCGCATCCCCATCAAGGTGGAGCAGGGCGAGGTGCTGAAGAAACCCGAGTGGATCCGCGTCAAGGCCGGCTCGC
CCACCACGCGTTTCTACGAGATCAAGGAGATCCTGCGCGAGCACAAGCTGCACACGGTGTGCGAGGAGGCCTCGTGCCCC
AACATCGGCGAGTGCTTCGGCAAGGGCACGGCCACGTTCATGATCATGGGCGACAAGTGCACGCGCCGCTGCCCGTTCTG
CGACGTGGGCCACGGCCGGCCGGACCCGCTGGACAAGGACGAACCGCTGAACCTCGCGCGCACCATCGCCGCGCTCAAGC
TGAAGTACGTGGTGATCACCAGCGTGGACCGCGACGACCTGCGCGACGGCGGCAGCGGCCACTTCGTGGAGTGCATCCAG
AACATCCGCGCGCTCTCGCCCGCCACGCAGATCGAGATCCTCGTGCCCGACTTCCGCGGCCGCGACGACCGCGCGCTGGA
GATCCTCAAGGCCGCGCCGCCCGACGTGATGAACCACAACCTGGAGACCGCGCCGCGCCTGTACAAGGAAGCGCGCCCGG
GATCGGACTACCAGTTCAGCCTGAACCTGCTCAAGAAGTTCAAGGCGCTGCACCCCGGCGTGCCCACCAAGAGCGGCATC
ATGGTCGGCCTGGGCGAGACCGATGAAGAGATCCTGCAGGTGATGCGCGACATGCGCGCGCACGACATCGACATGCTGAC
CATCGGCCAGTACCTCGCGCCGTCCAACAGCCACCTGCCGGTGCGCCGCTACGTGCACCCCGATACCTTCAAGATGTATG
AAGAGGAAGCCTACAAGATGGGCTTCACCCACGCCGCGGTGGGTGCGATGGTGCGTTCGAGCTACCACGCGGACCAGCAG
GCGCACGCGGCCGGCCTGCAGGCTGGCCCGCAGGGCTGA

Upstream 100 bases:

>100_bases
ACCTTTCTACAATCGGCGTGCACACCACCTGGGACGAAGCGGCTTCCGTGCTGGCCAGCCAATTGGCCATCCGCCTCGCG
CCCTGACCTCAGCACCAGCC

Downstream 100 bases:

>100_bases
ACCGCGGCGGCTGCGCGCTGGCGACGGGCCGGCGGGGGCCGCCGCCGCGATGGAAACGGAACGCGCGCCTCAGGAAGGCG
CCGTGATCCTCACGTAGCAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSTPEVVREAQSTVAYNPLAKQKAAAKLSRIPIKVEQGEVLKKPEWIRVKAGSPTTRFYEIKEILREHKLHTVCEEASCP
NIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDKDEPLNLARTIAALKLKYVVITSVDRDDLRDGGSGHFVECIQ
NIRALSPATQIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLNLLKKFKALHPGVPTKSGI
MVGLGETDEEILQVMRDMRAHDIDMLTIGQYLAPSNSHLPVRRYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHADQQ
AHAAGLQAGPQG

Sequences:

>Translated_332_residues
MSTPEVVREAQSTVAYNPLAKQKAAAKLSRIPIKVEQGEVLKKPEWIRVKAGSPTTRFYEIKEILREHKLHTVCEEASCP
NIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDKDEPLNLARTIAALKLKYVVITSVDRDDLRDGGSGHFVECIQ
NIRALSPATQIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLNLLKKFKALHPGVPTKSGI
MVGLGETDEEILQVMRDMRAHDIDMLTIGQYLAPSNSHLPVRRYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHADQQ
AHAAGLQAGPQG
>Mature_331_residues
STPEVVREAQSTVAYNPLAKQKAAAKLSRIPIKVEQGEVLKKPEWIRVKAGSPTTRFYEIKEILREHKLHTVCEEASCPN
IGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDKDEPLNLARTIAALKLKYVVITSVDRDDLRDGGSGHFVECIQN
IRALSPATQIEILVPDFRGRDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLNLLKKFKALHPGVPTKSGIM
VGLGETDEEILQVMRDMRAHDIDMLTIGQYLAPSNSHLPVRRYVHPDTFKMYEEEAYKMGFTHAAVGAMVRSSYHADQQA
HAAGLQAGPQG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=295, Percent_Identity=45.0847457627119, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=45.9143968871595, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1786846, Length=304, Percent_Identity=62.171052631579, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32564533, Length=297, Percent_Identity=42.0875420875421, Blast_Score=227, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=44.3298969072165, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI221513272, Length=297, Percent_Identity=46.4646464646465, Blast_Score=254, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_ACIAC (A1TJ31)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_968743.1
- ProteinModelPortal:   A1TJ31
- STRING:   A1TJ31
- GeneID:   4665325
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_0362
- NMPDR:   fig|397945.5.peg.326
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   A1TJ31
- ProtClustDB:   PRK05481
- BioCyc:   AAVE397945:AAVE_0362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36948; Mature: 36817

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPEVVREAQSTVAYNPLAKQKAAAKLSRIPIKVEQGEVLKKPEWIRVKAGSPTTRFYE
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEECCCCCHHHHH
IKEILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDKD
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCC
EPLNLARTIAALKLKYVVITSVDRDDLRDGGSGHFVECIQNIRALSPATQIEILVPDFRG
CCHHHHHHHHHHHEEEEEEEECCHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
RDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLNLLKKFKALHPGVPTKSGI
CCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCE
MVGLGETDEEILQVMRDMRAHDIDMLTIGQYLAPSNSHLPVRRYVHPDTFKMYEEEAYKM
EEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GFTHAAVGAMVRSSYHADQQAHAAGLQAGPQG
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
STPEVVREAQSTVAYNPLAKQKAAAKLSRIPIKVEQGEVLKKPEWIRVKAGSPTTRFYE
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEECCCCCHHHHH
IKEILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDKD
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCC
EPLNLARTIAALKLKYVVITSVDRDDLRDGGSGHFVECIQNIRALSPATQIEILVPDFRG
CCHHHHHHHHHHHEEEEEEEECCHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
RDDRALEILKAAPPDVMNHNLETAPRLYKEARPGSDYQFSLNLLKKFKALHPGVPTKSGI
CCHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCE
MVGLGETDEEILQVMRDMRAHDIDMLTIGQYLAPSNSHLPVRRYVHPDTFKMYEEEAYKM
EEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GFTHAAVGAMVRSSYHADQQAHAAGLQAGPQG
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA