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The map label for this gene is emrB [H]

Identifier: 120609025

GI number: 120609025

Start: 354091

End: 355659

Strand: Reverse

Name: emrB [H]

Synonym: Aave_0322

Alternate gene names: 120609025

Gene position: 355659-354091 (Counterclockwise)

Preceding gene: 120609026

Following gene: 120609023

Centisome position: 6.64

GC content: 68.2

Gene sequence:

>1569_bases
ATGTCCTCTCCCACCCACACACCGCCCCTGGCGCCGCCGCCGCTCGAAGGCAGCGCGCGCATGTGGGGCACGCTGGCCCT
CTCGGCGGCCACATTCATGAACGTGCTGGACACGTCGATCGCCAACGTGTCGCTGCCCGCCATCGCGGGCGACCTGGGCG
TGAGCCCCACGCAGGGCACCTGGGTCATCACCAGCTTCGCGGTGGCCAACGCCATCGCGGTGCCGCTCACCGGCTGGCTG
TCGCAGCGCTTCGGGCAGGTGCGCCTGTTCGTGGCCAGCGTGACGCTCTTCGTCATCGCATCGCTGCTGTGCGGGCTGGC
GCCCAACATGGCCACGCTCATCGCCTTCCGCGCACTGCAGGGTTTCGTGGCCGGGCCGATGATCCCGCTGTCGCAGTCGC
TGCTGCTTTCCAGCTACCCCAAGGCGCTGGCCGGGCTGGCCATGGCGATGTGGTCGATGACCACGCTCATCGCGCCGGTC
ATGGGGCCGTTGCTGGGCGGCTGGATCACGGACAACATGACCTGGCCGTGGATCTTCTACATCAACGTGCCGGTGGGCAT
CGTGGCGGTGCTCGTCACCTGGGGCATATTCCACAAGCGCGAATCCGAGCGGCGCGCGCTGCCGATCGACTCCGTCGGCC
TGGCCCTGCTCGTCATCTGGGTGGCGGCGATGCAGGTGATGCTGGACATCGGCAAGGAGCATGACTGGTTCGCTTCGCCC
TGGGTCGTGGCCTGCTGTGTGGTGGCCGTGGTGGGCTTCATCACCTTCATGGTCTGGGAGCGCGGCGAGGAGCACCCCGT
GGTGGACCTGTCGCTCTTCAAGATCCGGAATTTCTGGGCGGGCGCGCTGGCCACCTCGGTGGGCTACGGGCTGTTCTTCG
GCAACGTGGTGCTGCTGCCGCTCTGGCTGCAGCAGTACATGGGCTACACGGCCACGCAGGCGGGCATGGTGATGGCGCCG
GTGGGCCTGCTGGCGCTGGTGCTCTCCCCGGTGGTGGGCAAGAACATCGCCAAGGTGGACCCGCGCCGTTTCGCGACCTT
CGCCTTCCTCGTGTTCGCGCTGGTGCTGTGGATGCGCTCGCGGTTCAATACGCAGGCGGACCTGGCGACCATCATGGTGC
CCACCATCATCCAGGGCATCGCCATGGCGTTCTTCTTCATCCCGCTGGTGACGCTCACGCTGTCGGAGATCGCGCCGGCG
CGCATTCCGTCGGCGTCCGGGCTGTCGAACTTCATGCGCATCACGGCCGGGGCCATCGGCACGTCCGTGTCCACCACGCT
GTGGGAGCGGCGTGCCACGCTGCACCATGCGCAGTTGACCGAGGGCCTGCAGCAGGGCGGGGTGGCCGTCACGCAGACGC
TGCAAGGGCTGCAGGCGGGCGGATTGACGGCGGAGCAGGCGCTCGCGCAGGTCGAGCGGCTGGTGAACCAGCAGGCCTTC
ATGCTGGCGGCCAACGACATCTTTTATGCGTCGGCCGTGCTGTTCCTGCTGCTGATTCCGCTGGTCTGGCTGGCCCACCC
GGTACGTGCCAGTGCCGGCAGCGCGGACGCGGCGGCGGGTGCGCATTGA

Upstream 100 bases:

>100_bases
TGGCCCGCATCGTCGCAGCCAATGCAGGGCCGGCCCCCCGTGCCGGCAGCCGGACGCAGGCCCACTGACCGCGGCGGCGC
CCTCCCCTTTCGGAGCCTCC

Downstream 100 bases:

>100_bases
CTGGACCTCTCCCCTGAGCGGCTGCAGGGGGGACGACGCCAGTGGCCCGGCAGAGCCGGTTCCACGGCGTCGGTAGCCCT
CAGCCCAGGTACTTCGCGAA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSSPTHTPPLAPPPLEGSARMWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPTQGTWVITSFAVANAIAVPLTGWL
SQRFGQVRLFVASVTLFVIASLLCGLAPNMATLIAFRALQGFVAGPMIPLSQSLLLSSYPKALAGLAMAMWSMTTLIAPV
MGPLLGGWITDNMTWPWIFYINVPVGIVAVLVTWGIFHKRESERRALPIDSVGLALLVIWVAAMQVMLDIGKEHDWFASP
WVVACCVVAVVGFITFMVWERGEEHPVVDLSLFKIRNFWAGALATSVGYGLFFGNVVLLPLWLQQYMGYTATQAGMVMAP
VGLLALVLSPVVGKNIAKVDPRRFATFAFLVFALVLWMRSRFNTQADLATIMVPTIIQGIAMAFFFIPLVTLTLSEIAPA
RIPSASGLSNFMRITAGAIGTSVSTTLWERRATLHHAQLTEGLQQGGVAVTQTLQGLQAGGLTAEQALAQVERLVNQQAF
MLAANDIFYASAVLFLLLIPLVWLAHPVRASAGSADAAAGAH

Sequences:

>Translated_522_residues
MSSPTHTPPLAPPPLEGSARMWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPTQGTWVITSFAVANAIAVPLTGWL
SQRFGQVRLFVASVTLFVIASLLCGLAPNMATLIAFRALQGFVAGPMIPLSQSLLLSSYPKALAGLAMAMWSMTTLIAPV
MGPLLGGWITDNMTWPWIFYINVPVGIVAVLVTWGIFHKRESERRALPIDSVGLALLVIWVAAMQVMLDIGKEHDWFASP
WVVACCVVAVVGFITFMVWERGEEHPVVDLSLFKIRNFWAGALATSVGYGLFFGNVVLLPLWLQQYMGYTATQAGMVMAP
VGLLALVLSPVVGKNIAKVDPRRFATFAFLVFALVLWMRSRFNTQADLATIMVPTIIQGIAMAFFFIPLVTLTLSEIAPA
RIPSASGLSNFMRITAGAIGTSVSTTLWERRATLHHAQLTEGLQQGGVAVTQTLQGLQAGGLTAEQALAQVERLVNQQAF
MLAANDIFYASAVLFLLLIPLVWLAHPVRASAGSADAAAGAH
>Mature_521_residues
SSPTHTPPLAPPPLEGSARMWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPTQGTWVITSFAVANAIAVPLTGWLS
QRFGQVRLFVASVTLFVIASLLCGLAPNMATLIAFRALQGFVAGPMIPLSQSLLLSSYPKALAGLAMAMWSMTTLIAPVM
GPLLGGWITDNMTWPWIFYINVPVGIVAVLVTWGIFHKRESERRALPIDSVGLALLVIWVAAMQVMLDIGKEHDWFASPW
VVACCVVAVVGFITFMVWERGEEHPVVDLSLFKIRNFWAGALATSVGYGLFFGNVVLLPLWLQQYMGYTATQAGMVMAPV
GLLALVLSPVVGKNIAKVDPRRFATFAFLVFALVLWMRSRFNTQADLATIMVPTIIQGIAMAFFFIPLVTLTLSEIAPAR
IPSASGLSNFMRITAGAIGTSVSTTLWERRATLHHAQLTEGLQQGGVAVTQTLQGLQAGGLTAEQALAQVERLVNQQAFM
LAANDIFYASAVLFLLLIPLVWLAHPVRASAGSADAAAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=495, Percent_Identity=50.3030303030303, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=44.3339960238569, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=29.8507462686567, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788392, Length=420, Percent_Identity=27.6190476190476, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081983, Length=403, Percent_Identity=25.8064516129032, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789152, Length=210, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=132, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=167, Percent_Identity=26.3473053892216, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55843; Mature: 55711

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTHTPPLAPPPLEGSARMWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPTQGT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC
WVITSFAVANAIAVPLTGWLSQRFGQVRLFVASVTLFVIASLLCGLAPNMATLIAFRALQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GFVAGPMIPLSQSLLLSSYPKALAGLAMAMWSMTTLIAPVMGPLLGGWITDNMTWPWIFY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEE
INVPVGIVAVLVTWGIFHKRESERRALPIDSVGLALLVIWVAAMQVMLDIGKEHDWFASP
EECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
WVVACCVVAVVGFITFMVWERGEEHPVVDLSLFKIRNFWAGALATSVGYGLFFGNVVLLP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWLQQYMGYTATQAGMVMAPVGLLALVLSPVVGKNIAKVDPRRFATFAFLVFALVLWMRS
HHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHH
RFNTQADLATIMVPTIIQGIAMAFFFIPLVTLTLSEIAPARIPSASGLSNFMRITAGAIG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
TSVSTTLWERRATLHHAQLTEGLQQGGVAVTQTLQGLQAGGLTAEQALAQVERLVNQQAF
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHH
MLAANDIFYASAVLFLLLIPLVWLAHPVRASAGSADAAAGAH
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSPTHTPPLAPPPLEGSARMWGTLALSAATFMNVLDTSIANVSLPAIAGDLGVSPTQGT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCC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HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]