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The map label for this gene is gatB [H]

Identifier: 120608994

GI number: 120608994

Start: 322440

End: 323894

Strand: Reverse

Name: gatB [H]

Synonym: Aave_0291

Alternate gene names: 120608994

Gene position: 323894-322440 (Counterclockwise)

Preceding gene: 120608995

Following gene: 120608991

Centisome position: 6.05

GC content: 68.04

Gene sequence:

>1455_bases
ATGACGACCAGCAAACTGATCCATGGCTACGAAGTCGTCATCGGCTTCGAGACCCACGCCCAGCTCGCCACGCAATCGAA
GATCTTCAGCCGCGCGCCCACGGCCTTCGGCGCCGAGCCCAACACCCAGGCCTGCGCCGTGGACCTGGCCCTGCCCGGCA
CGCTGCCCGTGATGAACCGCGAGGCCGTGGCCTGCGCCATCCGCCTGGGCCTGGCCCTGGGCTCCAGGGTCGCGCCCGAG
AGCATCTTCGCGCGCAAGAACTACTTCTATCCCGACCTGCCCAAGGGCTACCAGATCAGCCAGTTCGAGATCCCGGTGGT
GCAGGGCGGCGAGGTGTCGTTCTATCTCGGCGACGAGAAGAAGACCGTGCGCCTGGTGCGCGCCCACCTGGAGGAAGACG
CGGGCAAGTCGCTGCACGAGGATTTCATCGGCCAGTCGGGCATCGACCTGAACCGTGCCGGCACGCCGCTGCTGGAGATC
GTGACCGAGCCCGACATCCGCAGCAGCGAGGAGGCCGTGGCCTATGCCCGCGAGCTGCACAAGATCGTGACCTGGATCGG
CATCTGCGACGGCAACATGCAGGAAGGCTCCTTCCGCTGCGACGCCAACGTCTCCGTGCGCAGGCCAGGGGCGCCGCTGG
GCACGCGCCGCGAGATCAAGAACCTGAACAGCTTCAGGTTCATGCAGCAGGCGATCGACTACGAGATCCGCTGGCAGATC
GAACAGATCGAGGACGGCCACGCGATCCAGCAGGCCACCGTGCTGTTCGACCCGGACACGGGCGAGACGCGCGCCATGCG
CACCAAGGAAGACGCGGCCGACTACCGCTACTTCCCCGACCCGGACCTGCCGCCGCTGCGGATTTCGGAGCAGTGGGTCC
AGGAAGAGCGCGCGCGCATGCCCGAACTACCGCGCACCATGGCCGCGCGCTTCGTGGCCGACTACGGCCTGCCGGAATAC
GACGCGACCACGCTCACGCAGTCCAAGGCCATGGCGGCCTACTTCGAGGAGGCGGCCAGGGCCAGCGGCAGCCCCAAGCT
CGCCAGCAACTGGATCATGGGCGAGGTGTCGCGCCGCGTGAACGCGGGCGAGACCGACATCGCCGCCGCGCCGGTGACGG
CCGCGCTGCTGGCGAAGCTGATCCAGCGCATCGCCGACGGCACCATCTCCAACGCCGCCGCCCGGCAGGTGTTCGAGGCG
CTCTGGACCGGCGAAGGCACTGACGTGGACGCGGTGATCGAGACCAGGGGCCTCAAGCAGATGAACGACTCCGGCGCGCT
GGAGAAGATCATCGACGAGGTGATCGCCGCCAATCCCGACAACGTGGCGCAGTTCAAGGCCGGCAAGGGCAAGGCGTTCA
ACGCCCTGGTGGGCCAGGCCATGAAGGCCAGCAAGGGCAAGGCGAACCCGCAGCAGGTCAACGAGATGCTGCGCGCGCGG
CTCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CTCATCGGCAACTATTTCCAGGAAAGCCGGCTGCTGAACGCCGCGCACCGCCTGCAGCAGGCGACCGATTTCCACCTCCG
CACGCCCGGAGGCCTGTGAC

Downstream 100 bases:

>100_bases
CGCTGCTGCGTATCGGGGCCGGCCGGAGCCCCGGTCAGCGAGAGGCCGGCACGGCCGGCATGCCCTGCTGCGCGGCCCAG
AGCTGGCGCAAATGGGCCAG

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTTSKLIHGYEVVIGFETHAQLATQSKIFSRAPTAFGAEPNTQACAVDLALPGTLPVMNREAVACAIRLGLALGSRVAPE
SIFARKNYFYPDLPKGYQISQFEIPVVQGGEVSFYLGDEKKTVRLVRAHLEEDAGKSLHEDFIGQSGIDLNRAGTPLLEI
VTEPDIRSSEEAVAYARELHKIVTWIGICDGNMQEGSFRCDANVSVRRPGAPLGTRREIKNLNSFRFMQQAIDYEIRWQI
EQIEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLRISEQWVQEERARMPELPRTMAARFVADYGLPEY
DATTLTQSKAMAAYFEEAARASGSPKLASNWIMGEVSRRVNAGETDIAAAPVTAALLAKLIQRIADGTISNAAARQVFEA
LWTGEGTDVDAVIETRGLKQMNDSGALEKIIDEVIAANPDNVAQFKAGKGKAFNALVGQAMKASKGKANPQQVNEMLRAR
LAAA

Sequences:

>Translated_484_residues
MTTSKLIHGYEVVIGFETHAQLATQSKIFSRAPTAFGAEPNTQACAVDLALPGTLPVMNREAVACAIRLGLALGSRVAPE
SIFARKNYFYPDLPKGYQISQFEIPVVQGGEVSFYLGDEKKTVRLVRAHLEEDAGKSLHEDFIGQSGIDLNRAGTPLLEI
VTEPDIRSSEEAVAYARELHKIVTWIGICDGNMQEGSFRCDANVSVRRPGAPLGTRREIKNLNSFRFMQQAIDYEIRWQI
EQIEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLRISEQWVQEERARMPELPRTMAARFVADYGLPEY
DATTLTQSKAMAAYFEEAARASGSPKLASNWIMGEVSRRVNAGETDIAAAPVTAALLAKLIQRIADGTISNAAARQVFEA
LWTGEGTDVDAVIETRGLKQMNDSGALEKIIDEVIAANPDNVAQFKAGKGKAFNALVGQAMKASKGKANPQQVNEMLRAR
LAAA
>Mature_483_residues
TTSKLIHGYEVVIGFETHAQLATQSKIFSRAPTAFGAEPNTQACAVDLALPGTLPVMNREAVACAIRLGLALGSRVAPES
IFARKNYFYPDLPKGYQISQFEIPVVQGGEVSFYLGDEKKTVRLVRAHLEEDAGKSLHEDFIGQSGIDLNRAGTPLLEIV
TEPDIRSSEEAVAYARELHKIVTWIGICDGNMQEGSFRCDANVSVRRPGAPLGTRREIKNLNSFRFMQQAIDYEIRWQIE
QIEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLRISEQWVQEERARMPELPRTMAARFVADYGLPEYD
ATTLTQSKAMAAYFEEAARASGSPKLASNWIMGEVSRRVNAGETDIAAAPVTAALLAKLIQRIADGTISNAAARQVFEAL
WTGEGTDVDAVIETRGLKQMNDSGALEKIIDEVIAANPDNVAQFKAGKGKAFNALVGQAMKASKGKANPQQVNEMLRARL
AAA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=501, Percent_Identity=38.7225548902196, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI115534094, Length=295, Percent_Identity=41.6949152542373, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=512, Percent_Identity=31.640625, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=36.2348178137652, Blast_Score=316, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53021; Mature: 52889

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSKLIHGYEVVIGFETHAQLATQSKIFSRAPTAFGAEPNTQACAVDLALPGTLPVMNR
CCCCCEECCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCH
EAVACAIRLGLALGSRVAPESIFARKNYFYPDLPKGYQISQFEIPVVQGGEVSFYLGDEK
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEECEEEEEEEECCEEEEEECCCH
KTVRLVRAHLEEDAGKSLHEDFIGQSGIDLNRAGTPLLEIVTEPDIRSSEEAVAYARELH
HHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
KIVTWIGICDGNMQEGSFRCDANVSVRRPGAPLGTRREIKNLNSFRFMQQAIDYEIRWQI
HHHHHHHCCCCCCCCCCEEECCCCEEECCCCCCCCHHHHHCCHHHHHHHHHHCEEEEEEE
EQIEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLRISEQWVQEERARM
EECCCCCCEEEEEEEECCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
PELPRTMAARFVADYGLPEYDATTLTQSKAMAAYFEEAARASGSPKLASNWIMGEVSRRV
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NAGETDIAAAPVTAALLAKLIQRIADGTISNAAARQVFEALWTGEGTDVDAVIETRGLKQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCHH
MNDSGALEKIIDEVIAANPDNVAQFKAGKGKAFNALVGQAMKASKGKANPQQVNEMLRAR
CCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LAAA
HHCC
>Mature Secondary Structure 
TTSKLIHGYEVVIGFETHAQLATQSKIFSRAPTAFGAEPNTQACAVDLALPGTLPVMNR
CCCCEECCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCH
EAVACAIRLGLALGSRVAPESIFARKNYFYPDLPKGYQISQFEIPVVQGGEVSFYLGDEK
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEECEEEEEEEECCEEEEEECCCH
KTVRLVRAHLEEDAGKSLHEDFIGQSGIDLNRAGTPLLEIVTEPDIRSSEEAVAYARELH
HHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
KIVTWIGICDGNMQEGSFRCDANVSVRRPGAPLGTRREIKNLNSFRFMQQAIDYEIRWQI
HHHHHHHCCCCCCCCCCEEECCCCEEECCCCCCCCHHHHHCCHHHHHHHHHHCEEEEEEE
EQIEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLRISEQWVQEERARM
EECCCCCCEEEEEEEECCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCC
PELPRTMAARFVADYGLPEYDATTLTQSKAMAAYFEEAARASGSPKLASNWIMGEVSRRV
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NAGETDIAAAPVTAALLAKLIQRIADGTISNAAARQVFEALWTGEGTDVDAVIETRGLKQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCHH
MNDSGALEKIIDEVIAANPDNVAQFKAGKGKAFNALVGQAMKASKGKANPQQVNEMLRAR
CCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LAAA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA