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The map label for this gene is gatA [H]

Identifier: 120608995

GI number: 120608995

Start: 323896

End: 325395

Strand: Reverse

Name: gatA [H]

Synonym: Aave_0292

Alternate gene names: 120608995

Gene position: 325395-323896 (Counterclockwise)

Preceding gene: 120608996

Following gene: 120608994

Centisome position: 6.08

GC content: 71.4

Gene sequence:

>1500_bases
ATGAGCGAACCCTCCTCTTCCCTGCACACCCTGGGCGTGGCCGAACTGGCCGCGGCGCTGCGCGGCAAGCGCATCTCCGC
CGTCGAGGCCGCCCGGCATTTCCTGGCCCGCATCCAGCAGCACACCGACCTCGGCGCCTTCGTGAGCGTCAACGAGGAGG
CCACCCTGGCCCGGGCCCGCGTCCAGGATGCGGCCATCGCCGCCGGCACGGCCGGCCCGCTGGCGGGCGTGCCCATCGCC
CACAAGGACATCTTCGTCACGCGCGACTTCCCCAGCACGGCCGGCTCGAAGATGCTGGCCGGCTACCAGTCGCCCTTCGA
CGCCACGGTCGTCACGCGGCTGGCCGACGCCGGCGCCGTGACGCTGGGCAAGCTCAACTGCGATGAATTCGCCATGGGCT
CGGCCAACGAGAACTCGGCCGTCGCCCCCCTGGGCGCCGATGCGCCCGCCCCGGTACGCAACCCCTGGGCCACCGACCGC
GTGCCGGGCGGCTCCTCCGGCGGCAGCGCCGTGGCCGTGGCCGCGCGCCTCGCCCCGGCCGTGACGGGCACCGACACCGG
CGGCTCCATCCGCCAGCCGGCCTCGTTCTGCGGCATCACCGGCATCAAGCCCACCTACGGCCGTGCCAGCCGCTACGGCA
TGATCGCCTTCGCCTCCAGCCTGGACCAGGCCGGCCCGATGGCCCGCTCGGCCGAGGACTGCGCCCTGCTGCTCTCGGCG
ATCTGCGGCCCCGACCCGGACCGCGACTCCACCAGCCTGGACGTGCCGGCCGAGGACTTCAGCGCGAAGCTGGGCGACAG
CATCGACGGCCTGCGCATCGGCATCCCGGCAGAGTTCTTCGGCGAGGGCCTGGCGCCCGACGTGCGCGCCGCGGTGGATG
GCGCGCTCAAGGAATACGAGAAGCTCGGCGCGAAGCTCGTGCCGATCACGCTGCCGCGCACCGAGCTGTCCATCCCGGTG
TACTACATCATCGCGCCGGCCGAGGCCTCGTCCAACCTGAGCCGCTTCGACGGCGTGAAGTTCGGCCACCGCGCCAGGGA
CTACACCGACCTGGTGGACATGTACAAGAAGACCCGCGCCGAGGGCTTCGGCGACGAGGTCAAGCGCCGCATCATGATCG
GCACCTACGTGCTCTCGCATGGCTACTACGACGCCTACTACCTGCAGGCGCAGAAGATCCGCCGCATGATCGCGGATGAC
TTCCAGAACGCCTTCAGGGACTGCGACCTGATCGCCGGCCCCGTGGCGCCCACGGTGGCCTGGAAGCTCGGCGAGCACGG
CAACGACCCGCTGGCCGACTACCTGGCCGACATCTTCACGCTGCCCGCGTCGCTCGCCGGCCTGCCCGGCATGAGCGTGC
CCGCCGGTTTCGGCGAAGGCGGCATGCCCGTGGGCCTGCAGCTCATCGGCAACTATTTCCAGGAAAGCCGGCTGCTGAAC
GCCGCGCACCGCCTGCAGCAGGCGACCGATTTCCACCTCCGCACGCCCGGAGGCCTGTGA

Upstream 100 bases:

>100_bases
GACCAGCGCGAGGCCAACCAGCGCAGCGCGCCCGCCGTGGAGCGGGGCCTGTTCCTGGTGCCCAAGGTGATCGAATAACA
AGAATAAAAGTACGTAATCC

Downstream 100 bases:

>100_bases
CATGACGACCAGCAAACTGATCCATGGCTACGAAGTCGTCATCGGCTTCGAGACCCACGCCCAGCTCGCCACGCAATCGA
AGATCTTCAGCCGCGCGCCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSEPSSSLHTLGVAELAAALRGKRISAVEAARHFLARIQQHTDLGAFVSVNEEATLARARVQDAAIAAGTAGPLAGVPIA
HKDIFVTRDFPSTAGSKMLAGYQSPFDATVVTRLADAGAVTLGKLNCDEFAMGSANENSAVAPLGADAPAPVRNPWATDR
VPGGSSGGSAVAVAARLAPAVTGTDTGGSIRQPASFCGITGIKPTYGRASRYGMIAFASSLDQAGPMARSAEDCALLLSA
ICGPDPDRDSTSLDVPAEDFSAKLGDSIDGLRIGIPAEFFGEGLAPDVRAAVDGALKEYEKLGAKLVPITLPRTELSIPV
YYIIAPAEASSNLSRFDGVKFGHRARDYTDLVDMYKKTRAEGFGDEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRMIADD
FQNAFRDCDLIAGPVAPTVAWKLGEHGNDPLADYLADIFTLPASLAGLPGMSVPAGFGEGGMPVGLQLIGNYFQESRLLN
AAHRLQQATDFHLRTPGGL

Sequences:

>Translated_499_residues
MSEPSSSLHTLGVAELAAALRGKRISAVEAARHFLARIQQHTDLGAFVSVNEEATLARARVQDAAIAAGTAGPLAGVPIA
HKDIFVTRDFPSTAGSKMLAGYQSPFDATVVTRLADAGAVTLGKLNCDEFAMGSANENSAVAPLGADAPAPVRNPWATDR
VPGGSSGGSAVAVAARLAPAVTGTDTGGSIRQPASFCGITGIKPTYGRASRYGMIAFASSLDQAGPMARSAEDCALLLSA
ICGPDPDRDSTSLDVPAEDFSAKLGDSIDGLRIGIPAEFFGEGLAPDVRAAVDGALKEYEKLGAKLVPITLPRTELSIPV
YYIIAPAEASSNLSRFDGVKFGHRARDYTDLVDMYKKTRAEGFGDEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRMIADD
FQNAFRDCDLIAGPVAPTVAWKLGEHGNDPLADYLADIFTLPASLAGLPGMSVPAGFGEGGMPVGLQLIGNYFQESRLLN
AAHRLQQATDFHLRTPGGL
>Mature_498_residues
SEPSSSLHTLGVAELAAALRGKRISAVEAARHFLARIQQHTDLGAFVSVNEEATLARARVQDAAIAAGTAGPLAGVPIAH
KDIFVTRDFPSTAGSKMLAGYQSPFDATVVTRLADAGAVTLGKLNCDEFAMGSANENSAVAPLGADAPAPVRNPWATDRV
PGGSSGGSAVAVAARLAPAVTGTDTGGSIRQPASFCGITGIKPTYGRASRYGMIAFASSLDQAGPMARSAEDCALLLSAI
CGPDPDRDSTSLDVPAEDFSAKLGDSIDGLRIGIPAEFFGEGLAPDVRAAVDGALKEYEKLGAKLVPITLPRTELSIPVY
YIIAPAEASSNLSRFDGVKFGHRARDYTDLVDMYKKTRAEGFGDEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRMIADDF
QNAFRDCDLIAGPVAPTVAWKLGEHGNDPLADYLADIFTLPASLAGLPGMSVPAGFGEGGMPVGLQLIGNYFQESRLLNA
AHRLQQATDFHLRTPGGL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=35.9840954274354, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=25.2485089463221, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI166795287, Length=278, Percent_Identity=32.7338129496403, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=449, Percent_Identity=38.9755011135858, Blast_Score=284, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI71990152, Length=305, Percent_Identity=28.5245901639344, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=315, Percent_Identity=25.3968253968254, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=25.6578947368421, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=326, Percent_Identity=26.0736196319018, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=314, Percent_Identity=27.0700636942675, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=325, Percent_Identity=24.9230769230769, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=35.4988399071926, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=425, Percent_Identity=31.0588235294118, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=37.6753507014028, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24652985, Length=504, Percent_Identity=26.1904761904762, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922090, Length=504, Percent_Identity=26.1904761904762, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652981, Length=504, Percent_Identity=26.1904761904762, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652983, Length=504, Percent_Identity=26.1904761904762, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24644968, Length=513, Percent_Identity=25.7309941520468, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078093, Length=511, Percent_Identity=26.027397260274, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24648435, Length=521, Percent_Identity=24.3761996161228, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24648437, Length=521, Percent_Identity=24.3761996161228, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI45550774, Length=521, Percent_Identity=24.3761996161228, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356731, Length=512, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=445, Percent_Identity=24.0449438202247, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=445, Percent_Identity=24.0449438202247, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52432; Mature: 52301

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPSSSLHTLGVAELAAALRGKRISAVEAARHFLARIQQHTDLGAFVSVNEEATLARAR
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
VQDAAIAAGTAGPLAGVPIAHKDIFVTRDFPSTAGSKMLAGYQSPFDATVVTRLADAGAV
HHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCE
TLGKLNCDEFAMGSANENSAVAPLGADAPAPVRNPWATDRVPGGSSGGSAVAVAARLAPA
EEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
VTGTDTGGSIRQPASFCGITGIKPTYGRASRYGMIAFASSLDQAGPMARSAEDCALLLSA
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
ICGPDPDRDSTSLDVPAEDFSAKLGDSIDGLRIGIPAEFFGEGLAPDVRAAVDGALKEYE
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCHHHHCCCCCCHHHHHHHHHHHHHH
KLGAKLVPITLPRTELSIPVYYIIAPAEASSNLSRFDGVKFGHRARDYTDLVDMYKKTRA
HCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EGFGDEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRMIADDFQNAFRDCDLIAGPVAPTVA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHH
WKLGEHGNDPLADYLADIFTLPASLAGLPGMSVPAGFGEGGMPVGLQLIGNYFQESRLLN
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AAHRLQQATDFHLRTPGGL
HHHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
SEPSSSLHTLGVAELAAALRGKRISAVEAARHFLARIQQHTDLGAFVSVNEEATLARAR
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
VQDAAIAAGTAGPLAGVPIAHKDIFVTRDFPSTAGSKMLAGYQSPFDATVVTRLADAGAV
HHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCE
TLGKLNCDEFAMGSANENSAVAPLGADAPAPVRNPWATDRVPGGSSGGSAVAVAARLAPA
EEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
VTGTDTGGSIRQPASFCGITGIKPTYGRASRYGMIAFASSLDQAGPMARSAEDCALLLSA
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
ICGPDPDRDSTSLDVPAEDFSAKLGDSIDGLRIGIPAEFFGEGLAPDVRAAVDGALKEYE
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCHHHHCCCCCCHHHHHHHHHHHHHH
KLGAKLVPITLPRTELSIPVYYIIAPAEASSNLSRFDGVKFGHRARDYTDLVDMYKKTRA
HCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EGFGDEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRMIADDFQNAFRDCDLIAGPVAPTVA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHH
WKLGEHGNDPLADYLADIFTLPASLAGLPGMSVPAGFGEGGMPVGLQLIGNYFQESRLLN
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AAHRLQQATDFHLRTPGGL
HHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA