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The map label for this gene is rstB [H]

Identifier: 120608977

GI number: 120608977

Start: 304282

End: 305523

Strand: Reverse

Name: rstB [H]

Synonym: Aave_0274

Alternate gene names: 120608977

Gene position: 305523-304282 (Counterclockwise)

Preceding gene: 120608978

Following gene: 120608975

Centisome position: 5.71

GC content: 72.79

Gene sequence:

>1242_bases
ATGTCCAATCCCTTTTCCCGCCGCCTCTACCTGCGCATCTGGCTCGCCGTGGTCGGCGGCATGGCGGTGCTCACGCTGGC
CGTGGGCTGGGCGTGGCGCATCGCGTCGGAGCAGAACGCGCTGACCCAGCCGGCGCCGCCGCCGCGCGAGATGGTGCTGC
TGGACGCCGCGGGCCAGCCCGTGCTGCGCGGTCTCGCGATCCGCGTGCCGCCGACGGCCGGCGGGCCACCGGGCGGGCTG
GAGTTCAGCGTGGAGGCGGAGAACGGCCAGTTGCCCTATACGCTGCAGTTCTCGCCGCGCATCCGCCCCCCCGGCGAGCG
GGACCGGCGCGGCGGTCCCGAGGCCGCTTTCTGGACGCGGCCGCCCTTCGGCTTCCTCTGGATGCTCGGCATCGTGGGCG
TCGCGGTGGCCGTGGGGGTGTTCCCCATCATCCGCCGGCTGCTCAAGCGGCTGGAGAACCTGCAGCGCGGCGTGCAGCGC
TTCGGCGAGGGCGACCTGTCGGTGCGCGTGGCCGAGCATGGCCAGGACGAGGTGGCCGACCTCGCACGGCAGTTCAACGC
CGCGGCCGCGCGCATCGAGACCCTGGTGAAGTCGCACAAGTCGCTGCTGGCGAATGCGTCGCACGAGCTGCGTTCGCCGC
TCACGCGCATCCGCATGGGGCTGGAGCTGATGCAGGGCAGCACACCCTCGCCGGCGTTCCGCTCCGAGATCCTGCGCAAC
ATCGCCGAGCTCGACCAGCTGGTGGACGAGATCCTGCTCGCCAGTCGCCTGGATGCGCGCGAGGCCGACATGGGCACCGT
GGAAACGGTGGATTTGATGGGCCTGGCCGCCGAGGAATGCGTGCGCGTGGACGCCGAACTGCAGGCCGCCGATGGCACCG
CCGCCGATGCGCTGGAAGTGCGCGGCATCGCCAAGCTGCTGCGCCGCGCGCTGCGCAACCTGCTGGAGAACGCGCGGCGC
TACAGCCAGGGCGACATCACCGTCACGCTGCGCAGCACGGGGACGCAGGTGGAGGTGAGCGTGTGCGACCAGGGCGCCGG
CGTGCCGCCGGCGCAGCGCGAACGCATCTTCGAGCCCTTCTACCGCCTGCCCGGCGCCAGTGAACGCTCGGGCGGCGTCG
GCCTGGGGCTGGCCCTGGTGCGCTCCATCGCGGAGCGGCACGACGGCAGTGTGTACTGCACGGACCGGCCCGACGGCGCG
GAGGGCGCCTGCTTCGTTCTGCGGCTGCCGCGCGCCACCTGA

Upstream 100 bases:

>100_bases
CGCACGGGTGGCGGCGGGGCGCGGTTCACGGGCCGCCTGCAATGCCGCAGGCCGCAGCATCAGCCGCCATGGCGTGACAT
CGCCACATTCTGAAACGCCC

Downstream 100 bases:

>100_bases
CCACCGCCCGCCCGCGCGGTGCTGCGCGTCAGTCCCCTGCCGGCAGCGGCTCCGGCGGCCAGCGCCACGCCATCGCGCCC
AGCACCGCCGCTCCCAGCCC

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSNPFSRRLYLRIWLAVVGGMAVLTLAVGWAWRIASEQNALTQPAPPPREMVLLDAAGQPVLRGLAIRVPPTAGGPPGGL
EFSVEAENGQLPYTLQFSPRIRPPGERDRRGGPEAAFWTRPPFGFLWMLGIVGVAVAVGVFPIIRRLLKRLENLQRGVQR
FGEGDLSVRVAEHGQDEVADLARQFNAAAARIETLVKSHKSLLANASHELRSPLTRIRMGLELMQGSTPSPAFRSEILRN
IAELDQLVDEILLASRLDAREADMGTVETVDLMGLAAEECVRVDAELQAADGTAADALEVRGIAKLLRRALRNLLENARR
YSQGDITVTLRSTGTQVEVSVCDQGAGVPPAQRERIFEPFYRLPGASERSGGVGLGLALVRSIAERHDGSVYCTDRPDGA
EGACFVLRLPRAT

Sequences:

>Translated_413_residues
MSNPFSRRLYLRIWLAVVGGMAVLTLAVGWAWRIASEQNALTQPAPPPREMVLLDAAGQPVLRGLAIRVPPTAGGPPGGL
EFSVEAENGQLPYTLQFSPRIRPPGERDRRGGPEAAFWTRPPFGFLWMLGIVGVAVAVGVFPIIRRLLKRLENLQRGVQR
FGEGDLSVRVAEHGQDEVADLARQFNAAAARIETLVKSHKSLLANASHELRSPLTRIRMGLELMQGSTPSPAFRSEILRN
IAELDQLVDEILLASRLDAREADMGTVETVDLMGLAAEECVRVDAELQAADGTAADALEVRGIAKLLRRALRNLLENARR
YSQGDITVTLRSTGTQVEVSVCDQGAGVPPAQRERIFEPFYRLPGASERSGGVGLGLALVRSIAERHDGSVYCTDRPDGA
EGACFVLRLPRAT
>Mature_412_residues
SNPFSRRLYLRIWLAVVGGMAVLTLAVGWAWRIASEQNALTQPAPPPREMVLLDAAGQPVLRGLAIRVPPTAGGPPGGLE
FSVEAENGQLPYTLQFSPRIRPPGERDRRGGPEAAFWTRPPFGFLWMLGIVGVAVAVGVFPIIRRLLKRLENLQRGVQRF
GEGDLSVRVAEHGQDEVADLARQFNAAAARIETLVKSHKSLLANASHELRSPLTRIRMGLELMQGSTPSPAFRSEILRNI
AELDQLVDEILLASRLDAREADMGTVETVDLMGLAAEECVRVDAELQAADGTAADALEVRGIAKLLRRALRNLLENARRY
SQGDITVTLRSTGTQVEVSVCDQGAGVPPAQRERIFEPFYRLPGASERSGGVGLGLALVRSIAERHDGSVYCTDRPDGAE
GACFVLRLPRAT

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=294, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789808, Length=270, Percent_Identity=31.4814814814815, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790346, Length=245, Percent_Identity=33.0612244897959, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=30.0724637681159, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786783, Length=299, Percent_Identity=29.7658862876254, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082128, Length=306, Percent_Identity=28.1045751633987, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=32.7433628318584, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786600, Length=234, Percent_Identity=30.3418803418803, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790861, Length=313, Percent_Identity=29.3929712460064, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790551, Length=316, Percent_Identity=26.2658227848101, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=30.6382978723404, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081816, Length=252, Percent_Identity=29.3650793650794, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789403, Length=225, Percent_Identity=33.7777777777778, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=28.310502283105, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789149, Length=261, Percent_Identity=26.4367816091954, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI145693157, Length=262, Percent_Identity=25.9541984732824, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44870; Mature: 44739

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPFSRRLYLRIWLAVVGGMAVLTLAVGWAWRIASEQNALTQPAPPPREMVLLDAAGQP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEECCCCH
VLRGLAIRVPPTAGGPPGGLEFSVEAENGQLPYTLQFSPRIRPPGERDRRGGPEAAFWTR
HHCCEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PPFGFLWMLGIVGVAVAVGVFPIIRRLLKRLENLQRGVQRFGEGDLSVRVAEHGQDEVAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCHHHHH
LARQFNAAAARIETLVKSHKSLLANASHELRSPLTRIRMGLELMQGSTPSPAFRSEILRN
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IAELDQLVDEILLASRLDAREADMGTVETVDLMGLAAEECVRVDAELQAADGTAADALEV
HHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHH
RGIAKLLRRALRNLLENARRYSQGDITVTLRSTGTQVEVSVCDQGAGVPPAQRERIFEPF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHH
YRLPGASERSGGVGLGLALVRSIAERHDGSVYCTDRPDGAEGACFVLRLPRAT
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SNPFSRRLYLRIWLAVVGGMAVLTLAVGWAWRIASEQNALTQPAPPPREMVLLDAAGQP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEECCCCH
VLRGLAIRVPPTAGGPPGGLEFSVEAENGQLPYTLQFSPRIRPPGERDRRGGPEAAFWTR
HHCCEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PPFGFLWMLGIVGVAVAVGVFPIIRRLLKRLENLQRGVQRFGEGDLSVRVAEHGQDEVAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCHHHHH
LARQFNAAAARIETLVKSHKSLLANASHELRSPLTRIRMGLELMQGSTPSPAFRSEILRN
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IAELDQLVDEILLASRLDAREADMGTVETVDLMGLAAEECVRVDAELQAADGTAADALEV
HHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHH
RGIAKLLRRALRNLLENARRYSQGDITVTLRSTGTQVEVSVCDQGAGVPPAQRERIFEPF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHH
YRLPGASERSGGVGLGLALVRSIAERHDGSVYCTDRPDGAEGACFVLRLPRAT
HHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]