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The map label for this gene is yneJ [H]

Identifier: 120608975

GI number: 120608975

Start: 302015

End: 302899

Strand: Reverse

Name: yneJ [H]

Synonym: Aave_0272

Alternate gene names: 120608975

Gene position: 302899-302015 (Counterclockwise)

Preceding gene: 120608977

Following gene: 120608974

Centisome position: 5.66

GC content: 75.25

Gene sequence:

>885_bases
ATGGACCTGTCCGCCCTGGAAATCTTTCGCGCCGTCGCAGCCGAAGGCAGCGTCACCCGCGCGGCCGAGCGGCTGGGCCG
GGTGCAGTCGAACGTGACCACCCGCGTGCAGCAGTTGGAGGAACAGCTGGGTGCCACGCTGTTCCTGCGCGAAGGCCGGC
GCATGGCGCTGACCCCGGCCGGTACCTCGCTGGTGGCCTATGCCGACCGCCTGCTGGCGCTGGCGGAGGAAGCCCGCCAG
GCCCTGCACCCCGGCACCCCCGGCGGCAGGCTGCGCCTGGGCGCCATGGAAAGCACCGCCGCCGCCCGCCTGCCCGCCCC
GCTCGCCCGCCTGCACGCCCGATGGCCCGGCCTGCAACTGGAACTGCGCACGGGCGCGTCGCGCGAGTTGGTGGACCAGG
TCCTGGCCCACGCCAGCGACTGCGCCCTGGTCGCCTGGCCGCCGCCGGGCGTGGATCCGGAAGCGCCGGTGGACTGCACT
CCCGTCTTCACCGAATCGCTGCTCATCGCCCTGCCGGCGGGCCATCCACCGGTGGAGCGCCCGGGAGACCTGCAGGTGGA
CACCCTGGCCGCGTTCTCTCCGGGCTGCACCTACCGCCGCCTGGGCGAGGACTGGATGCGCGCGCAGCGGCACGGCGCCG
CGCCGCACATACTGGAGCTGGCCTCCTACCATGCCATCCTGGCCTGCGTGGCCGCGGGGCGCTGCGCCGGCGTGGTGCCG
CAGTCCGTCATCGACCTGATGCGCGAACCGCCCGCGCTGCGCTGGGTGCCGCTGGCCGGGCGCGAGACGGTGCTGGTGCG
CCGCCGCGGCTGGCACACCCCGGCCCTGGATGCGCTGCTGGATGCGCTGCGCGACGTCCCTGCCGGCATGCCGCCGGGCG
CCTGA

Upstream 100 bases:

>100_bases
GGGGCCGGGCGGAGAGGTATCGATGGCCATGGAGTTTTCGCTTCTTTAATTCTCTTTTTAAGATTAACATTCAATCGATT
CATCTTCAAACAAGATATTC

Downstream 100 bases:

>100_bases
AACCGACCACCCGCTGTTCCCAGCCCCCCCGGAGACCCGCCATGCCGACGCCCGTCCCTCCATCCCGCCGCACTCCGCTG
TTCGACCGCCTGGGCCTCAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDLSALEIFRAVAAEGSVTRAAERLGRVQSNVTTRVQQLEEQLGATLFLREGRRMALTPAGTSLVAYADRLLALAEEARQ
ALHPGTPGGRLRLGAMESTAAARLPAPLARLHARWPGLQLELRTGASRELVDQVLAHASDCALVAWPPPGVDPEAPVDCT
PVFTESLLIALPAGHPPVERPGDLQVDTLAAFSPGCTYRRLGEDWMRAQRHGAAPHILELASYHAILACVAAGRCAGVVP
QSVIDLMREPPALRWVPLAGRETVLVRRRGWHTPALDALLDALRDVPAGMPPGA

Sequences:

>Translated_294_residues
MDLSALEIFRAVAAEGSVTRAAERLGRVQSNVTTRVQQLEEQLGATLFLREGRRMALTPAGTSLVAYADRLLALAEEARQ
ALHPGTPGGRLRLGAMESTAAARLPAPLARLHARWPGLQLELRTGASRELVDQVLAHASDCALVAWPPPGVDPEAPVDCT
PVFTESLLIALPAGHPPVERPGDLQVDTLAAFSPGCTYRRLGEDWMRAQRHGAAPHILELASYHAILACVAAGRCAGVVP
QSVIDLMREPPALRWVPLAGRETVLVRRRGWHTPALDALLDALRDVPAGMPPGA
>Mature_294_residues
MDLSALEIFRAVAAEGSVTRAAERLGRVQSNVTTRVQQLEEQLGATLFLREGRRMALTPAGTSLVAYADRLLALAEEARQ
ALHPGTPGGRLRLGAMESTAAARLPAPLARLHARWPGLQLELRTGASRELVDQVLAHASDCALVAWPPPGVDPEAPVDCT
PVFTESLLIALPAGHPPVERPGDLQVDTLAAFSPGCTYRRLGEDWMRAQRHGAAPHILELASYHAILACVAAGRCAGVVP
QSVIDLMREPPALRWVPLAGRETVLVRRRGWHTPALDALLDALRDVPAGMPPGA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=41.7808219178082, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI87082132, Length=236, Percent_Identity=29.6610169491525, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=27.8169014084507, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787879, Length=273, Percent_Identity=27.1062271062271, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788626, Length=148, Percent_Identity=31.7567567567568, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994962, Length=189, Percent_Identity=31.7460317460317, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=26.510067114094, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790208, Length=295, Percent_Identity=25.0847457627119, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788481, Length=165, Percent_Identity=31.5151515151515, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788887, Length=177, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=180, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=205, Percent_Identity=25.3658536585366, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=216, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31439; Mature: 31439

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSALEIFRAVAAEGSVTRAAERLGRVQSNVTTRVQQLEEQLGATLFLREGRRMALTPA
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GTSLVAYADRLLALAEEARQALHPGTPGGRLRLGAMESTAAARLPAPLARLHARWPGLQL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHCCHHHHHHHHCCCCCEE
ELRTGASRELVDQVLAHASDCALVAWPPPGVDPEAPVDCTPVFTESLLIALPAGHPPVER
EECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCC
PGDLQVDTLAAFSPGCTYRRLGEDWMRAQRHGAAPHILELASYHAILACVAAGRCAGVVP
CCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHCCCH
QSVIDLMREPPALRWVPLAGRETVLVRRRGWHTPALDALLDALRDVPAGMPPGA
HHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLSALEIFRAVAAEGSVTRAAERLGRVQSNVTTRVQQLEEQLGATLFLREGRRMALTPA
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GTSLVAYADRLLALAEEARQALHPGTPGGRLRLGAMESTAAARLPAPLARLHARWPGLQL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHCCHHHHHHHHCCCCCEE
ELRTGASRELVDQVLAHASDCALVAWPPPGVDPEAPVDCTPVFTESLLIALPAGHPPVER
EECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCC
PGDLQVDTLAAFSPGCTYRRLGEDWMRAQRHGAAPHILELASYHAILACVAAGRCAGVVP
CCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHCCCH
QSVIDLMREPPALRWVPLAGRETVLVRRRGWHTPALDALLDALRDVPAGMPPGA
HHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]