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The map label for this gene is metR [H]

Identifier: 120608911

GI number: 120608911

Start: 227632

End: 228651

Strand: Reverse

Name: metR [H]

Synonym: Aave_0204

Alternate gene names: 120608911

Gene position: 228651-227632 (Counterclockwise)

Preceding gene: 120608914

Following gene: 120608910

Centisome position: 4.27

GC content: 70.78

Gene sequence:

>1020_bases
ATGAATAAAAGCGATGAATCGACGCCTGCCATGATGACCTTCAAGCAACTGGAAGCCCTGTACTGGATCGGCCAGCTCGG
CGGCTTCGCCCCCGCCGCCCGCAAGCTGCACACCACGCAGTCCGCCATCTCCAAGCGGGTGCACGAGCTGGAACAGGTCT
TCGACACCGCCCTGTTCGACCGCAGCCAGCGCAGCGCGCGCCTGACGGAAAAAGGCGAGGAGATGTTCATGCTGGCGCGC
AAGCTGCTGCGCGAGCGCGATGCGGCGGTGGAGCAGTTCGGCAAGCCCGGGGTGATCGAACGGCGCCTGCGCATCGGCGT
GACGGAGCTCACGGCCATGACCTGGCTGCCGCGCCTGGTGGGCCTGGTGCGCGCGCACTACCCCAAGGTCACGCTGGAGC
CCGACGTGGACGGCAGCCTGCCCCTGCGCGACAAGCTGCTGGCCGACGCGCTGGACCTCGTCATCGTGCCCGACGCTTTT
GCCGACAGCCGCCTGCCCGCCAAGCCCGTGGGCCAGGTCGAGAGCGCCTGGATGTGCCGCCCCGGCCTCGTGCAGCCCCC
CGCCCCCGGCCGCCCCGTGCGCCTGCACGACCTGGCCGCCCACCGGCTGCTGGTGCAGGGCAACGCCTCCGGCACCGGCC
GCATCTACGACGGCTGGATGCGCGAGCGCGGCGTGGAGCCCGCGGACACTCTCGTGGTCAGCAACCTGATCGCGCTGGCC
GGCCTCACCATCTCCGGCCTGGGCGTGAGCTACCTGCCCCGCCACTGCCTCGCCCCCCTGGTCGCCGCCGGCATGCTGGC
CGAGATCGAGGTGCAGCCACCGCTGCCGCCCGTGCCCTATGTGGCGATGGTGCGAGGGGAGCAGCGCAGCGCCCTGGTGG
CGTCGGTGATCATGCTGGCGCAGTCGTGCTGCGACTTCACGCGGGTGTTCCAGGCGGGGGTGGATGTGGCCAGTGCAGAG
CCCGGTCGCATGGAATCTCGACCAGGACCATTCCGGCAGGGCCGCGGTGCGAGCGCGTGA

Upstream 100 bases:

>100_bases
ACGGAGGAAAAGCCGTGGGCGGTCAGCGCCACGGCCGGGATGCATCCCATTGTGAAAGCCGCGTTATTTCCCGAAAAGCG
ATTTTTTGTGCTCGAATCGC

Downstream 100 bases:

>100_bases
CAACCTTCTGAAAGCGCTTCCTGCCCTGCCCCATGCCAACGATGGCCACCCCATCGACATCCTCGCCCACGACCAGGCCG
CCTGGGACCGCCAGGCCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNKSDESTPAMMTFKQLEALYWIGQLGGFAPAARKLHTTQSAISKRVHELEQVFDTALFDRSQRSARLTEKGEEMFMLAR
KLLRERDAAVEQFGKPGVIERRLRIGVTELTAMTWLPRLVGLVRAHYPKVTLEPDVDGSLPLRDKLLADALDLVIVPDAF
ADSRLPAKPVGQVESAWMCRPGLVQPPAPGRPVRLHDLAAHRLLVQGNASGTGRIYDGWMRERGVEPADTLVVSNLIALA
GLTISGLGVSYLPRHCLAPLVAAGMLAEIEVQPPLPPVPYVAMVRGEQRSALVASVIMLAQSCCDFTRVFQAGVDVASAE
PGRMESRPGPFRQGRGASA

Sequences:

>Translated_339_residues
MNKSDESTPAMMTFKQLEALYWIGQLGGFAPAARKLHTTQSAISKRVHELEQVFDTALFDRSQRSARLTEKGEEMFMLAR
KLLRERDAAVEQFGKPGVIERRLRIGVTELTAMTWLPRLVGLVRAHYPKVTLEPDVDGSLPLRDKLLADALDLVIVPDAF
ADSRLPAKPVGQVESAWMCRPGLVQPPAPGRPVRLHDLAAHRLLVQGNASGTGRIYDGWMRERGVEPADTLVVSNLIALA
GLTISGLGVSYLPRHCLAPLVAAGMLAEIEVQPPLPPVPYVAMVRGEQRSALVASVIMLAQSCCDFTRVFQAGVDVASAE
PGRMESRPGPFRQGRGASA
>Mature_339_residues
MNKSDESTPAMMTFKQLEALYWIGQLGGFAPAARKLHTTQSAISKRVHELEQVFDTALFDRSQRSARLTEKGEEMFMLAR
KLLRERDAAVEQFGKPGVIERRLRIGVTELTAMTWLPRLVGLVRAHYPKVTLEPDVDGSLPLRDKLLADALDLVIVPDAF
ADSRLPAKPVGQVESAWMCRPGLVQPPAPGRPVRLHDLAAHRLLVQGNASGTGRIYDGWMRERGVEPADTLVVSNLIALA
GLTISGLGVSYLPRHCLAPLVAAGMLAEIEVQPPLPPVPYVAMVRGEQRSALVASVIMLAQSCCDFTRVFQAGVDVASAE
PGRMESRPGPFRQGRGASA

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=296, Percent_Identity=26.0135135135135, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082132, Length=260, Percent_Identity=25, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788626, Length=263, Percent_Identity=28.5171102661597, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789639, Length=197, Percent_Identity=30.4568527918782, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=25.6944444444444, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=259, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=223, Percent_Identity=26.9058295964126, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=21.4285714285714, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36927; Mature: 36927

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSDESTPAMMTFKQLEALYWIGQLGGFAPAARKLHTTQSAISKRVHELEQVFDTALFD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSQRSARLTEKGEEMFMLARKLLRERDAAVEQFGKPGVIERRLRIGVTELTAMTWLPRLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLVRAHYPKVTLEPDVDGSLPLRDKLLADALDLVIVPDAFADSRLPAKPVGQVESAWMCR
HHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCHHHHHCCC
PGLVQPPAPGRPVRLHDLAAHRLLVQGNASGTGRIYDGWMRERGVEPADTLVVSNLIALA
CCCCCCCCCCCCEEHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
GLTISGLGVSYLPRHCLAPLVAAGMLAEIEVQPPLPPVPYVAMVRGEQRSALVASVIMLA
CCHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHH
QSCCDFTRVFQAGVDVASAEPGRMESRPGPFRQGRGASA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKSDESTPAMMTFKQLEALYWIGQLGGFAPAARKLHTTQSAISKRVHELEQVFDTALFD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSQRSARLTEKGEEMFMLARKLLRERDAAVEQFGKPGVIERRLRIGVTELTAMTWLPRLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLVRAHYPKVTLEPDVDGSLPLRDKLLADALDLVIVPDAFADSRLPAKPVGQVESAWMCR
HHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCHHHHHCCC
PGLVQPPAPGRPVRLHDLAAHRLLVQGNASGTGRIYDGWMRERGVEPADTLVVSNLIALA
CCCCCCCCCCCCEEHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
GLTISGLGVSYLPRHCLAPLVAAGMLAEIEVQPPLPPVPYVAMVRGEQRSALVASVIMLA
CCHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHH
QSCCDFTRVFQAGVDVASAEPGRMESRPGPFRQGRGASA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]