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The map label for this gene is nylA [H]

Identifier: 120608899

GI number: 120608899

Start: 215415

End: 216893

Strand: Reverse

Name: nylA [H]

Synonym: Aave_0191

Alternate gene names: 120608899

Gene position: 216893-215415 (Counterclockwise)

Preceding gene: 120608900

Following gene: 120608898

Centisome position: 4.05

GC content: 72.14

Gene sequence:

>1479_bases
ATGGGCTTGGCGATGGGTTGGGACGAATGGGCCGCGCACGACGCGGTGGGGCTGGCCGGACTGGTGCGCCAGGGCGAGGT
GACACCCGTCGAACTGGCCGCCCAGGCGGCGGCCGGCGTGGCGAAGGTCAATCCCGCCCTGAGCGCGGTGGTCGAGGTCT
TCGCCGATGCGGTGGCCGATCCGCAGCAGGCCGGCATGCAGGCCGGCGGCCCGCTGGCCGGCGTACCCTTCTTCATGAAG
GACCTCGGCCCCACCCTGGCCGGGCGGCTGCAGGAACAGGGCTCGCTCTTCATGCGCGGCCACCGCCCCACCGAAGACGC
CTGGCTCACGCAGCAGATGCGCCGCGCGGGCCTGAACCTGATGGGCCGCACCACCACCCCGGAATTCGGCGTGTGCAGCT
CGGCCGAGAACCCGGCCGTCTATGTCACGCGCAACCCCTGGAACACCGGCTACACCACCTGCGGCTCGTCCGCCGGCACG
GCGGCCATCGTGGCGGCGGGCGTGGTGCCGATGTCGCATGCCACCGACGGCGGCGGCTCCATCCGCATCCCCGCGGGCTT
CAACGGCAACATCGGCCTCAAGCCTTCGCGCGGCGTGTTCTCCATCGCGCCCCATGCCTCGGACATCACGGGCTACGTCT
CCACCCAGGGCTGCCACAGCCGCACGGTGCGCGACACGGCGGCCTTCGTCGATGCCTGCCGCGGCGGCGCGCCGGGCGAG
TTCATGCCCTACTGGACGGCGCCCGAGCCCTACACGGAACTGATCCGGCGCGACCCGGCCCGGCTGCGCATCGCCCTCTC
GCACGAATGGGGCGACTACCGCGCCACGCCCGCCATCGTGGCGGAACTGGAAAAGGCGGGCCGCTTCCTCGAAGGCCTGG
GCCACCAGGTGGAGTGGGCGCTGCCGCAGGTGGACCTGCGCGCCGCCTTCCGCGCCCAGACCACCTGCTACATCAGCAAC
TTCGCCCAGGTCATCAACGGCCTGCTGGAGCGGCGCGGCCTGGAGCGCCCGCCCGCCGACCTGATCGAGCCCATCAACAT
CCGCATCTGGGAGGCGGGCCGCGGACTGCCCTACGCCGAGCGCCACCGCATGCAGGCCGTGTTCAACACCACGGCGCGCG
GCTTCGGCGCGTTCTTCGAGGACTGGGACATCCTCCTGACGCCCATCACCGCGCGGCCCACGCCGCCCGTGGGCACCACC
GAATACCTCACCACCAGCGGCAACCCCGACGTGCTGGACTGGTTCGACAACCTCTGGCGGTTCTTCGCGTACACGCCGCT
GTCCAACCTCTGCGGCATCCCGGCCATCTCGCTGCCCATGGGCGCGCAGGACCACGGCCTGCCCTTCGGCATCCACGCCC
AGGCGCGGCAGGGCCACGACGGCCTGCTGCTGCAGCTGGCAGCGCAGATCGAGCGCGCGCTGGACGGGCGCTGGAACGGT
GGCCGGCGGCCAGGGGTGCACGTGGCGCAGCCGCAGTAA

Upstream 100 bases:

>100_bases
GGCAGCCTGCAGGGCGGCCGTCCCAGGCGGCGCCGGTGGGGGCCCTGCGCGCGATGCGCGGTAACCTTGCCGGCGTACGA
CGCGAACGGAAGGCTTGCAC

Downstream 100 bases:

>100_bases
CGGCGGGGAAGCACAGCCCGGCAGTGGCGCGTTTTTCCCACGGCAGGGCACGCGGGGAGCGGGGTACAGTGGCCCATCGC
CCAGGAGACACCCACCGATG

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MGLAMGWDEWAAHDAVGLAGLVRQGEVTPVELAAQAAAGVAKVNPALSAVVEVFADAVADPQQAGMQAGGPLAGVPFFMK
DLGPTLAGRLQEQGSLFMRGHRPTEDAWLTQQMRRAGLNLMGRTTTPEFGVCSSAENPAVYVTRNPWNTGYTTCGSSAGT
AAIVAAGVVPMSHATDGGGSIRIPAGFNGNIGLKPSRGVFSIAPHASDITGYVSTQGCHSRTVRDTAAFVDACRGGAPGE
FMPYWTAPEPYTELIRRDPARLRIALSHEWGDYRATPAIVAELEKAGRFLEGLGHQVEWALPQVDLRAAFRAQTTCYISN
FAQVINGLLERRGLERPPADLIEPINIRIWEAGRGLPYAERHRMQAVFNTTARGFGAFFEDWDILLTPITARPTPPVGTT
EYLTTSGNPDVLDWFDNLWRFFAYTPLSNLCGIPAISLPMGAQDHGLPFGIHAQARQGHDGLLLQLAAQIERALDGRWNG
GRRPGVHVAQPQ

Sequences:

>Translated_492_residues
MGLAMGWDEWAAHDAVGLAGLVRQGEVTPVELAAQAAAGVAKVNPALSAVVEVFADAVADPQQAGMQAGGPLAGVPFFMK
DLGPTLAGRLQEQGSLFMRGHRPTEDAWLTQQMRRAGLNLMGRTTTPEFGVCSSAENPAVYVTRNPWNTGYTTCGSSAGT
AAIVAAGVVPMSHATDGGGSIRIPAGFNGNIGLKPSRGVFSIAPHASDITGYVSTQGCHSRTVRDTAAFVDACRGGAPGE
FMPYWTAPEPYTELIRRDPARLRIALSHEWGDYRATPAIVAELEKAGRFLEGLGHQVEWALPQVDLRAAFRAQTTCYISN
FAQVINGLLERRGLERPPADLIEPINIRIWEAGRGLPYAERHRMQAVFNTTARGFGAFFEDWDILLTPITARPTPPVGTT
EYLTTSGNPDVLDWFDNLWRFFAYTPLSNLCGIPAISLPMGAQDHGLPFGIHAQARQGHDGLLLQLAAQIERALDGRWNG
GRRPGVHVAQPQ
>Mature_491_residues
GLAMGWDEWAAHDAVGLAGLVRQGEVTPVELAAQAAAGVAKVNPALSAVVEVFADAVADPQQAGMQAGGPLAGVPFFMKD
LGPTLAGRLQEQGSLFMRGHRPTEDAWLTQQMRRAGLNLMGRTTTPEFGVCSSAENPAVYVTRNPWNTGYTTCGSSAGTA
AIVAAGVVPMSHATDGGGSIRIPAGFNGNIGLKPSRGVFSIAPHASDITGYVSTQGCHSRTVRDTAAFVDACRGGAPGEF
MPYWTAPEPYTELIRRDPARLRIALSHEWGDYRATPAIVAELEKAGRFLEGLGHQVEWALPQVDLRAAFRAQTTCYISNF
AQVINGLLERRGLERPPADLIEPINIRIWEAGRGLPYAERHRMQAVFNTTARGFGAFFEDWDILLTPITARPTPPVGTTE
YLTTSGNPDVLDWFDNLWRFFAYTPLSNLCGIPAISLPMGAQDHGLPFGIHAQARQGHDGLLLQLAAQIERALDGRWNGG
RRPGVHVAQPQ

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=517, Percent_Identity=24.5647969052224, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI195972892, Length=195, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=347, Percent_Identity=26.2247838616715, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=206, Percent_Identity=29.126213592233, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=276, Percent_Identity=25.7246376811594, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=305, Percent_Identity=27.5409836065574, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=422, Percent_Identity=24.4075829383886, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356731, Length=498, Percent_Identity=25.1004016064257, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24644968, Length=206, Percent_Identity=32.0388349514563, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=25, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45550774, Length=204, Percent_Identity=28.4313725490196, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648435, Length=204, Percent_Identity=28.4313725490196, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648437, Length=204, Percent_Identity=28.4313725490196, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=470, Percent_Identity=24.0425531914894, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652985, Length=207, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19922090, Length=207, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24652981, Length=207, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24652983, Length=207, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648441, Length=140, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648439, Length=140, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 52879; Mature: 52748

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAMGWDEWAAHDAVGLAGLVRQGEVTPVELAAQAAAGVAKVNPALSAVVEVFADAVAD
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHCC
PQQAGMQAGGPLAGVPFFMKDLGPTLAGRLQEQGSLFMRGHRPTEDAWLTQQMRRAGLNL
HHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCE
MGRTTTPEFGVCSSAENPAVYVTRNPWNTGYTTCGSSAGTAAIVAAGVVPMSHATDGGGS
EECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCEECCCCCCCCCE
IRIPAGFNGNIGLKPSRGVFSIAPHASDITGYVSTQGCHSRTVRDTAAFVDACRGGAPGE
EEECCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
FMPYWTAPEPYTELIRRDPARLRIALSHEWGDYRATPAIVAELEKAGRFLEGLGHQVEWA
CCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEC
LPQVDLRAAFRAQTTCYISNFAQVINGLLERRGLERPPADLIEPINIRIWEAGRGLPYAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCCCCHHH
RHRMQAVFNTTARGFGAFFEDWDILLTPITARPTPPVGTTEYLTTSGNPDVLDWFDNLWR
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
FFAYTPLSNLCGIPAISLPMGAQDHGLPFGIHAQARQGHDGLLLQLAAQIERALDGRWNG
HHHHCCHHHHCCCCEEECCCCCCCCCCCEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCCC
GRRPGVHVAQPQ
CCCCCEECCCCC
>Mature Secondary Structure 
GLAMGWDEWAAHDAVGLAGLVRQGEVTPVELAAQAAAGVAKVNPALSAVVEVFADAVAD
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHCC
PQQAGMQAGGPLAGVPFFMKDLGPTLAGRLQEQGSLFMRGHRPTEDAWLTQQMRRAGLNL
HHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCE
MGRTTTPEFGVCSSAENPAVYVTRNPWNTGYTTCGSSAGTAAIVAAGVVPMSHATDGGGS
EECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCEECCCCCCCCCE
IRIPAGFNGNIGLKPSRGVFSIAPHASDITGYVSTQGCHSRTVRDTAAFVDACRGGAPGE
EEECCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
FMPYWTAPEPYTELIRRDPARLRIALSHEWGDYRATPAIVAELEKAGRFLEGLGHQVEWA
CCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEC
LPQVDLRAAFRAQTTCYISNFAQVINGLLERRGLERPPADLIEPINIRIWEAGRGLPYAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCCCCHHH
RHRMQAVFNTTARGFGAFFEDWDILLTPITARPTPPVGTTEYLTTSGNPDVLDWFDNLWR
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
FFAYTPLSNLCGIPAISLPMGAQDHGLPFGIHAQARQGHDGLLLQLAAQIERALDGRWNG
HHHHCCHHHHCCCCEEECCCCCCCCCCCEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCCC
GRRPGVHVAQPQ
CCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]