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The map label for this gene is metH [H]

Identifier: 120608882

GI number: 120608882

Start: 194235

End: 195299

Strand: Reverse

Name: metH [H]

Synonym: Aave_0174

Alternate gene names: 120608882

Gene position: 195299-194235 (Counterclockwise)

Preceding gene: 120608883

Following gene: 120608881

Centisome position: 3.65

GC content: 68.83

Gene sequence:

>1065_bases
ATGCAACCGCTCCACTACACCCGCGCCCGGCAATTGCCTGGCATCCTGGCCGAACGCATCGCCATCCTCGACGGCGCCAT
GGGCACCATGATCCAGCGCTTCAAGCTGGGCGAGGCCCAGTACCGCGGCGAGGGCTACAGCGGCCCCGACGGTGCAGGCG
ACCGGTTCAAGGATTTCCCGCGGGACGTGAAAGGCAACAACGAGCTGCTCTCGCTCACGCGGCCCGACGTGATCCGCGAC
ATCCACGAGCGCTACCTGGCCGCAGGCGCCGACCTGATCGAGACCAACACCTTCGGCGCCACCACGATCGCGCAGGAGGA
CTACCACATGGCCGACCTGGCGCGGGAGATGAACCTCGCCTCGGCCCGGTTGGCGCGCGCCGCCTGCGACAAATACTCCA
CGCCCGACAAGCCGCGTTTCGTGGCCGGGGCCCTCGGGCCGACGCCCAAGACAGCGAGCATCAGCCCGGACGTGAACGAC
CCCGGCGCACGCAACGTCGATTTCGAGCAACTACGTGCCGCGTACTACGAGCAGACCCAGGCGCTGGTCGAAGGCGGTGC
CGACGTGCTGCTGGTGGAAACCATCTTCGACACGCTCAACGCCAAGGCCGCGCTCTTCGCCATCGACGAATACTTCGAGG
CGAGCGGCGAGCGCCTGCCGCTCATCATCAGCGGCACCGTCACCGATGCCTCCGGCCGCATCCTCTCGGGCCAGACCGTG
ACGGCCTTCTGGCACAGCGTGCGCCACGCGCGCCCGCTGGCCATCGGCCTGAACTGCGCCCTGGGCGCCACGCTGATGCG
CCCCTACATCCAGGAACTGAACCGGGTGGCGGAAGACACCTTCATCAGCTGCTACCCCAACGCCGGCCTGCCCAACCCCA
TGAGCGACACCGGCTTCGACGAGACGCCCGAGGTCACCAGCCGCCTGGTGCACGAGTTCGCCGCCGAGGGCCTGGTGAAC
ATCGTGGGCGGCTGCTGCGGCACCACGCCGGACCACATCGCCGCCATCGGCCGCGCCGTGGCGCCGGTGCCCACGCGCAA
GCTGTTCTACCCCGAAGCGGCCTGA

Upstream 100 bases:

>100_bases
AACCGGTGGTTGTGCAAAGCCATGGCGGAGTCAGGAGCAAAGGGGCGGAACCAGCACGGACAGGCCGGGGTGCGGGACGC
ACTTTCTACAATGGAGGCTT

Downstream 100 bases:

>100_bases
TCCCGCCCGGCCGGCCCACCCCGGAGCCGGCGGATGGGCTGCTCTCCTACCGCCGGCTCATCCGTGGCCCTTTAGGATGC
GCACGTCTTCCACCCGGATC

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MQPLHYTRARQLPGILAERIAILDGAMGTMIQRFKLGEAQYRGEGYSGPDGAGDRFKDFPRDVKGNNELLSLTRPDVIRD
IHERYLAAGADLIETNTFGATTIAQEDYHMADLAREMNLASARLARAACDKYSTPDKPRFVAGALGPTPKTASISPDVND
PGARNVDFEQLRAAYYEQTQALVEGGADVLLVETIFDTLNAKAALFAIDEYFEASGERLPLIISGTVTDASGRILSGQTV
TAFWHSVRHARPLAIGLNCALGATLMRPYIQELNRVAEDTFISCYPNAGLPNPMSDTGFDETPEVTSRLVHEFAAEGLVN
IVGGCCGTTPDHIAAIGRAVAPVPTRKLFYPEAA

Sequences:

>Translated_354_residues
MQPLHYTRARQLPGILAERIAILDGAMGTMIQRFKLGEAQYRGEGYSGPDGAGDRFKDFPRDVKGNNELLSLTRPDVIRD
IHERYLAAGADLIETNTFGATTIAQEDYHMADLAREMNLASARLARAACDKYSTPDKPRFVAGALGPTPKTASISPDVND
PGARNVDFEQLRAAYYEQTQALVEGGADVLLVETIFDTLNAKAALFAIDEYFEASGERLPLIISGTVTDASGRILSGQTV
TAFWHSVRHARPLAIGLNCALGATLMRPYIQELNRVAEDTFISCYPNAGLPNPMSDTGFDETPEVTSRLVHEFAAEGLVN
IVGGCCGTTPDHIAAIGRAVAPVPTRKLFYPEAA
>Mature_354_residues
MQPLHYTRARQLPGILAERIAILDGAMGTMIQRFKLGEAQYRGEGYSGPDGAGDRFKDFPRDVKGNNELLSLTRPDVIRD
IHERYLAAGADLIETNTFGATTIAQEDYHMADLAREMNLASARLARAACDKYSTPDKPRFVAGALGPTPKTASISPDVND
PGARNVDFEQLRAAYYEQTQALVEGGADVLLVETIFDTLNAKAALFAIDEYFEASGERLPLIISGTVTDASGRILSGQTV
TAFWHSVRHARPLAIGLNCALGATLMRPYIQELNRVAEDTFISCYPNAGLPNPMSDTGFDETPEVTSRLVHEFAAEGLVN
IVGGCCGTTPDHIAAIGRAVAPVPTRKLFYPEAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=344, Percent_Identity=52.6162790697674, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI1790450, Length=344, Percent_Identity=56.6860465116279, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535405, Length=338, Percent_Identity=52.9585798816568, Blast_Score=347, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24585077, Length=282, Percent_Identity=25.531914893617, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38290; Mature: 38290

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLHYTRARQLPGILAERIAILDGAMGTMIQRFKLGEAQYRGEGYSGPDGAGDRFKDFP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHCC
RDVKGNNELLSLTRPDVIRDIHERYLAAGADLIETNTFGATTIAQEDYHMADLAREMNLA
CCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHHHH
SARLARAACDKYSTPDKPRFVAGALGPTPKTASISPDVNDPGARNVDFEQLRAAYYEQTQ
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALVEGGADVLLVETIFDTLNAKAALFAIDEYFEASGERLPLIISGTVTDASGRILSGQTV
HHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEECEECCCCCEECCCHH
TAFWHSVRHARPLAIGLNCALGATLMRPYIQELNRVAEDTFISCYPNAGLPNPMSDTGFD
HHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCC
ETPEVTSRLVHEFAAEGLVNIVGGCCGTTPDHIAAIGRAVAPVPTRKLFYPEAA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPLHYTRARQLPGILAERIAILDGAMGTMIQRFKLGEAQYRGEGYSGPDGAGDRFKDFP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHCC
RDVKGNNELLSLTRPDVIRDIHERYLAAGADLIETNTFGATTIAQEDYHMADLAREMNLA
CCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHHHH
SARLARAACDKYSTPDKPRFVAGALGPTPKTASISPDVNDPGARNVDFEQLRAAYYEQTQ
HHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALVEGGADVLLVETIFDTLNAKAALFAIDEYFEASGERLPLIISGTVTDASGRILSGQTV
HHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEECEECCCCCEECCCHH
TAFWHSVRHARPLAIGLNCALGATLMRPYIQELNRVAEDTFISCYPNAGLPNPMSDTGFD
HHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCC
ETPEVTSRLVHEFAAEGLVNIVGGCCGTTPDHIAAIGRAVAPVPTRKLFYPEAA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]