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The map label for this gene is pilS [H]

Identifier: 120608881

GI number: 120608881

Start: 192671

End: 194158

Strand: Reverse

Name: pilS [H]

Synonym: Aave_0173

Alternate gene names: 120608881

Gene position: 194158-192671 (Counterclockwise)

Preceding gene: 120608882

Following gene: 120608880

Centisome position: 3.63

GC content: 74.93

Gene sequence:

>1488_bases
ATGCGCACGTCTTCCACCCGGATCCCCCCCGTGCGCTCCCTGCGTATCCAACTCACCCTCTTCTGGACCCTGCTGCTGGG
CGCCTGCGCCCTGGTGGCGGTCGTCCTCACGGTGCTCTACCAGTCGGGCTCCGGCGCGCAGGTGGCCGCCGGCCGCACCC
GCGTGGCGCAGGCCTGCACCGACGTCGCCGGGCGCTACGCCCAGTCCGCGCCCGGCGTGGCGGCCGACGCCCCGCGCGAG
GACCTGCTGCGGGTGCTCCTGCACATGGTCCTGCTGGAGGCGCCGCGCGTCGAAGGCGGCGCCTGGAGCGCCAGCGGCGG
CATGCTGGCCTATGCCTACCCCACCTACGAAGGCAGCGGCGTGAAAAGCGACGTGCCCTCCGCCGAGGAACCCCTGATCG
TGGAGATGGCGCGACAGGCCAGCGCCGGGCGCCAGCCGCTGGTGAACCTGGTGCGCGGCAGCCGCGAGGTGCTCATCGTG
GCGGCCTGCCCGCTGGCCGCGCACCCCGGCACGGCCGTCTGGACCATGACCCGCGTGCATGCCGGCGCCATCGCCGCGCA
GGACAGCCTGCGCGCGGGCATCGCCATCCTGGCCCTGCTGGTGGCGGTGTCGGGCGCATGGCTCGGATGGATGCTGCAGC
GCGGCCTGCGGCAGGTGCAGCGCCTGGAGGACGCCCTGGCGCAGGCCGGCGGCGACACCGTCCCCGTGCTCCAGCGCACC
GGAATTCGCGAGCTCGACCGGATCGTGGAAGGCTTCAACAGCTTCGGCGCCCGGCTGCAGGAAGCGCGCGAGCGCCTGCG
CGAGGCGCTCGCCCAGCAGCATCGCGACATGCGCCTCACGGCCCTGGGCCGCATGACGGCCTCGGTGGCGCACGAAATCC
GCAACCCCATCGCCGCCATGCGGCTGAAGGCCGACAACGCCCTGGCGGCGCCCCCCGCCCGCCATGCCGACGCCCTGGCC
GCCATCGGCACGCAGATCGACCGGCTGGAGGGCCTGGTGCAGAGCCTGCTGACCCTGGTGCAGCCCCTGCAACTCGAACC
CCACCCGGTGGACCTGCCCGCCTGGCTGCAGGAGCGGCTGGACACCGCCCGGGCCGCGGCCGAGGCCCGGGGCGTGCGCC
TGTCGCTGCGCGGCGATGTACCGGCCACGGCCGTCTTCGACCCCGTGCACGCGGCCCGCGCCATCGACAACCTGCTGGAC
AACGCCGTGCGCCATGCGCCGCGGGGAGGCGAGGCCGTGCTCTCGGCCCAGGTGGATGCCGCCCGTTCCCTGTGCGTGAT
CGCGGTGGAGGACGACGGCCCGGGCGTGCCCGAAGCATTGCGCGCCCAGCTCTTCGAGCCCTTCGCCACCGGCCGCACCG
ACGGCAACGGCCTGGGCCTGGCCCTGGCGCGCGAGGTGGCGCTGGCGCACGGGGGCGACCTGCACTACGCGCCGCGCGAA
GGCGGCGGCGCCCGCTTCCTCCTGGAGCTGCCATGGCACGCATCCTAG

Upstream 100 bases:

>100_bases
CTGTTCTACCCCGAAGCGGCCTGATCCCGCCCGGCCGGCCCACCCCGGAGCCGGCGGATGGGCTGCTCTCCTACCGCCGG
CTCATCCGTGGCCCTTTAGG

Downstream 100 bases:

>100_bases
CCGTCGATGACGACGCGGCCTTCCGCGAATCGCTGGTCGAAACCCTGCAGGGCCTGGGGCACGAGGTGCACGAGGCCGCC
AGCGGCGACGAGGCGCTGCG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRTSSTRIPPVRSLRIQLTLFWTLLLGACALVAVVLTVLYQSGSGAQVAAGRTRVAQACTDVAGRYAQSAPGVAADAPRE
DLLRVLLHMVLLEAPRVEGGAWSASGGMLAYAYPTYEGSGVKSDVPSAEEPLIVEMARQASAGRQPLVNLVRGSREVLIV
AACPLAAHPGTAVWTMTRVHAGAIAAQDSLRAGIAILALLVAVSGAWLGWMLQRGLRQVQRLEDALAQAGGDTVPVLQRT
GIRELDRIVEGFNSFGARLQEARERLREALAQQHRDMRLTALGRMTASVAHEIRNPIAAMRLKADNALAAPPARHADALA
AIGTQIDRLEGLVQSLLTLVQPLQLEPHPVDLPAWLQERLDTARAAAEARGVRLSLRGDVPATAVFDPVHAARAIDNLLD
NAVRHAPRGGEAVLSAQVDAARSLCVIAVEDDGPGVPEALRAQLFEPFATGRTDGNGLGLALAREVALAHGGDLHYAPRE
GGGARFLLELPWHAS

Sequences:

>Translated_495_residues
MRTSSTRIPPVRSLRIQLTLFWTLLLGACALVAVVLTVLYQSGSGAQVAAGRTRVAQACTDVAGRYAQSAPGVAADAPRE
DLLRVLLHMVLLEAPRVEGGAWSASGGMLAYAYPTYEGSGVKSDVPSAEEPLIVEMARQASAGRQPLVNLVRGSREVLIV
AACPLAAHPGTAVWTMTRVHAGAIAAQDSLRAGIAILALLVAVSGAWLGWMLQRGLRQVQRLEDALAQAGGDTVPVLQRT
GIRELDRIVEGFNSFGARLQEARERLREALAQQHRDMRLTALGRMTASVAHEIRNPIAAMRLKADNALAAPPARHADALA
AIGTQIDRLEGLVQSLLTLVQPLQLEPHPVDLPAWLQERLDTARAAAEARGVRLSLRGDVPATAVFDPVHAARAIDNLLD
NAVRHAPRGGEAVLSAQVDAARSLCVIAVEDDGPGVPEALRAQLFEPFATGRTDGNGLGLALAREVALAHGGDLHYAPRE
GGGARFLLELPWHAS
>Mature_495_residues
MRTSSTRIPPVRSLRIQLTLFWTLLLGACALVAVVLTVLYQSGSGAQVAAGRTRVAQACTDVAGRYAQSAPGVAADAPRE
DLLRVLLHMVLLEAPRVEGGAWSASGGMLAYAYPTYEGSGVKSDVPSAEEPLIVEMARQASAGRQPLVNLVRGSREVLIV
AACPLAAHPGTAVWTMTRVHAGAIAAQDSLRAGIAILALLVAVSGAWLGWMLQRGLRQVQRLEDALAQAGGDTVPVLQRT
GIRELDRIVEGFNSFGARLQEARERLREALAQQHRDMRLTALGRMTASVAHEIRNPIAAMRLKADNALAAPPARHADALA
AIGTQIDRLEGLVQSLLTLVQPLQLEPHPVDLPAWLQERLDTARAAAEARGVRLSLRGDVPATAVFDPVHAARAIDNLLD
NAVRHAPRGGEAVLSAQVDAARSLCVIAVEDDGPGVPEALRAQLFEPFATGRTDGNGLGLALAREVALAHGGDLHYAPRE
GGGARFLLELPWHAS

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=32.0675105485232, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=31.0483870967742, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787894, Length=249, Percent_Identity=33.7349397590361, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786783, Length=313, Percent_Identity=27.7955271565495, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87081816, Length=309, Percent_Identity=29.4498381877023, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=319, Percent_Identity=29.153605015674, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1789808, Length=329, Percent_Identity=27.0516717325228, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87082128, Length=252, Percent_Identity=26.5873015873016, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789149, Length=284, Percent_Identity=25, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=250, Percent_Identity=25.2, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994928, Length=274, Percent_Identity=24.4525547445255, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=27.5261324041812, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790346, Length=227, Percent_Identity=28.6343612334802, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786600, Length=220, Percent_Identity=30, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789403, Length=297, Percent_Identity=31.6498316498317, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790300, Length=203, Percent_Identity=32.0197044334975, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786912, Length=260, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790551, Length=277, Percent_Identity=25.6317689530686, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52474; Mature: 52474

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSSTRIPPVRSLRIQLTLFWTLLLGACALVAVVLTVLYQSGSGAQVAAGRTRVAQACT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHH
DVAGRYAQSAPGVAADAPREDLLRVLLHMVLLEAPRVEGGAWSASGGMLAYAYPTYEGSG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCC
VKSDVPSAEEPLIVEMARQASAGRQPLVNLVRGSREVLIVAACPLAAHPGTAVWTMTRVH
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEHHHHH
AGAIAAQDSLRAGIAILALLVAVSGAWLGWMLQRGLRQVQRLEDALAQAGGDTVPVLQRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
GIRELDRIVEGFNSFGARLQEARERLREALAQQHRDMRLTALGRMTASVAHEIRNPIAAM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHH
RLKADNALAAPPARHADALAAIGTQIDRLEGLVQSLLTLVQPLQLEPHPVDLPAWLQERL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
DTARAAAEARGVRLSLRGDVPATAVFDPVHAARAIDNLLDNAVRHAPRGGEAVLSAQVDA
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ARSLCVIAVEDDGPGVPEALRAQLFEPFATGRTDGNGLGLALAREVALAHGGDLHYAPRE
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
GGGARFLLELPWHAS
CCCCEEEEECCCCCC
>Mature Secondary Structure
MRTSSTRIPPVRSLRIQLTLFWTLLLGACALVAVVLTVLYQSGSGAQVAAGRTRVAQACT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHH
DVAGRYAQSAPGVAADAPREDLLRVLLHMVLLEAPRVEGGAWSASGGMLAYAYPTYEGSG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCCCCC
VKSDVPSAEEPLIVEMARQASAGRQPLVNLVRGSREVLIVAACPLAAHPGTAVWTMTRVH
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEHHHHH
AGAIAAQDSLRAGIAILALLVAVSGAWLGWMLQRGLRQVQRLEDALAQAGGDTVPVLQRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
GIRELDRIVEGFNSFGARLQEARERLREALAQQHRDMRLTALGRMTASVAHEIRNPIAAM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHH
RLKADNALAAPPARHADALAAIGTQIDRLEGLVQSLLTLVQPLQLEPHPVDLPAWLQERL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
DTARAAAEARGVRLSLRGDVPATAVFDPVHAARAIDNLLDNAVRHAPRGGEAVLSAQVDA
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ARSLCVIAVEDDGPGVPEALRAQLFEPFATGRTDGNGLGLALAREVALAHGGDLHYAPRE
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
GGGARFLLELPWHAS
CCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]