Click here to switch to the map view.

The map label for this gene is thlA [H]

Identifier: 120608744

GI number: 120608744

Start: 34220

End: 35416

Strand: Reverse

Name: thlA [H]

Synonym: Aave_0031

Alternate gene names: 120608744

Gene position: 35416-34220 (Counterclockwise)

Preceding gene: 120608745

Following gene: 120608743

Centisome position: 0.66

GC content: 71.43

Gene sequence:

>1197_bases
ATGACTTCTGCGCACCGCGACATCCTCATCGCCTCCATCGCCCGCACCGCCATCGGCACCTTCGGCGGCTCGCTCAAGGA
CATTCCCAACACCCAGCTCGCCACCACCGCCGTGCGCGCGGCCATCGAGCGCAGCGGCATCGCGGCCGACGCGGTCGGCC
ACGTGGTGATGGGCAACGTGATCCCCACCGACACGAAGGACGCGTACCTCTCGCGCGTGGCCGCCATCGACGCGGGCTGC
CCCGTGGAGACGCCGGCCTTCAACGTCAACCGCCTGTGCGGCTCGGGCCTGCAGGCCATCGTGTCGGCGGCGCAGGCGAT
CGCGCTGGGCGACTGCGACGTGGCCATCGGCGGCGGCAGCGAATCGATGAGCCGCGGCCCCTACTTCGACACCGCTTCGC
GCTGGGGCGCGCGCATGGGCGATGCGAAGAGCATCGACTACATGCTGGGCATCCTGCACGATCCGTGGCAGAAGATGCAC
ATGGGCATCACCGCCGAGAACGTGGCCGAGCGCTACCGCATCGGCCGCGAAGCCCAGGACCAGCTGGCCGCGGAGAGCCA
GCGCCGCGCCGCCGCCGCCATCGCGGCGGGCTATTTCAAGGAGCAGATCGTCCCGGTGGAAATCCCCGGCCGCAAGGGCA
CGGCCACGCTGTTCGACACCGACGAGCACGTGCGCGGCGGCACCACGGTGGAGGTGCTGGCCGGCATGAAGCCCGCGTTC
AGGAAGGACGGCGGCACGGTCACCGCGGGCAACGCCTCGGGCATCAACGACGGCGCGGCCGCCGTGGTGCTGGTGGCGGG
CGACCGCGCTGCCGCGCTGGGCGCCAGGCCGCTGGCGCGCCTGGTCGGCTACGCGCATGCCGGGGTGGAACCGGCCTACA
TGGGCATCGGCCCGGTGCCCGCCACGCGCGCGGTGCTGCAGCGCACGGGCCTGTCGGTGCAGGACATGGATGTGATCGAA
GCCAACGAAGCCTTCGCGGCCCAGGCGTGCGCCGTGATCCAGGAACTGGGGCTGGACCCGGCCAAGGTGAACCCGAACGG
CTCGGGCATCTCGCTCGGCCACCCCGTCGGCGCCACGGGCGCCATCATCACCACCAAGGCCATCGCCGAGCTGCACCGCA
CGGGCGGCCGCTATGCGCTGGTCACCATGTGCATCGGTGGCGGCCAGGGCATCGCAGCCATTTTCGAGCGCGTTTGA

Upstream 100 bases:

>100_bases
CCTCGCGGCAGGACCGGGCCGTGCAATGCGATATACCTGACGCCATCACCCGCAGGGGCCCGGACGTGCATCACGGCCCC
TTGTTCCACCGGAGACCTCC

Downstream 100 bases:

>100_bases
AACGATTGGAAACATCCACGGCAAACCGGGCGAGGCCAACTAGGATCGCCTCCTTTGCGCGACCACCACACAACCGATGC
CATACCTCTACCAGAATGCC

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTSAHRDILIASIARTAIGTFGGSLKDIPNTQLATTAVRAAIERSGIAADAVGHVVMGNVIPTDTKDAYLSRVAAIDAGC
PVETPAFNVNRLCGSGLQAIVSAAQAIALGDCDVAIGGGSESMSRGPYFDTASRWGARMGDAKSIDYMLGILHDPWQKMH
MGITAENVAERYRIGREAQDQLAAESQRRAAAAIAAGYFKEQIVPVEIPGRKGTATLFDTDEHVRGGTTVEVLAGMKPAF
RKDGGTVTAGNASGINDGAAAVVLVAGDRAAALGARPLARLVGYAHAGVEPAYMGIGPVPATRAVLQRTGLSVQDMDVIE
ANEAFAAQACAVIQELGLDPAKVNPNGSGISLGHPVGATGAIITTKAIAELHRTGGRYALVTMCIGGGQGIAAIFERV

Sequences:

>Translated_398_residues
MTSAHRDILIASIARTAIGTFGGSLKDIPNTQLATTAVRAAIERSGIAADAVGHVVMGNVIPTDTKDAYLSRVAAIDAGC
PVETPAFNVNRLCGSGLQAIVSAAQAIALGDCDVAIGGGSESMSRGPYFDTASRWGARMGDAKSIDYMLGILHDPWQKMH
MGITAENVAERYRIGREAQDQLAAESQRRAAAAIAAGYFKEQIVPVEIPGRKGTATLFDTDEHVRGGTTVEVLAGMKPAF
RKDGGTVTAGNASGINDGAAAVVLVAGDRAAALGARPLARLVGYAHAGVEPAYMGIGPVPATRAVLQRTGLSVQDMDVIE
ANEAFAAQACAVIQELGLDPAKVNPNGSGISLGHPVGATGAIITTKAIAELHRTGGRYALVTMCIGGGQGIAAIFERV
>Mature_397_residues
TSAHRDILIASIARTAIGTFGGSLKDIPNTQLATTAVRAAIERSGIAADAVGHVVMGNVIPTDTKDAYLSRVAAIDAGCP
VETPAFNVNRLCGSGLQAIVSAAQAIALGDCDVAIGGGSESMSRGPYFDTASRWGARMGDAKSIDYMLGILHDPWQKMHM
GITAENVAERYRIGREAQDQLAAESQRRAAAAIAAGYFKEQIVPVEIPGRKGTATLFDTDEHVRGGTTVEVLAGMKPAFR
KDGGTVTAGNASGINDGAAAVVLVAGDRAAALGARPLARLVGYAHAGVEPAYMGIGPVPATRAVLQRTGLSVQDMDVIEA
NEAFAAQACAVIQELGLDPAKVNPNGSGISLGHPVGATGAIITTKAIAELHRTGGRYALVTMCIGGGQGIAAIFERV

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=45.1530612244898, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=42.3558897243108, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=39.5939086294416, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=41.8546365914787, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=32.3943661971831, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI194353979, Length=392, Percent_Identity=31.3775510204082, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=47.5247524752475, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788554, Length=396, Percent_Identity=48.2323232323232, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI87082165, Length=393, Percent_Identity=45.0381679389313, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=38.7909319899244, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=32.4390243902439, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI133906874, Length=396, Percent_Identity=46.7171717171717, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=35.8395989974937, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI25147385, Length=396, Percent_Identity=34.3434343434343, Blast_Score=227, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17551802, Length=425, Percent_Identity=30.1176470588235, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=27.970297029703, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=37.7171215880893, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=34.5, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24655093, Length=393, Percent_Identity=45.29262086514, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=43.75, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=37.6237623762376, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=29.5081967213115, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40867; Mature: 40736

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAHRDILIASIARTAIGTFGGSLKDIPNTQLATTAVRAAIERSGIAADAVGHVVMGNV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
IPTDTKDAYLSRVAAIDAGCPVETPAFNVNRLCGSGLQAIVSAAQAIALGDCDVAIGGGS
CCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEECCCC
ESMSRGPYFDTASRWGARMGDAKSIDYMLGILHDPWQKMHMGITAENVAERYRIGREAQD
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHHH
QLAAESQRRAAAAIAAGYFKEQIVPVEIPGRKGTATLFDTDEHVRGGTTVEVLAGMKPAF
HHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCCCCCCCEEHHHCCCHHH
RKDGGTVTAGNASGINDGAAAVVLVAGDRAAALGARPLARLVGYAHAGVEPAYMGIGPVP
HCCCCEEEECCCCCCCCCCEEEEEEECCCHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCH
ATRAVLQRTGLSVQDMDVIEANEAFAAQACAVIQELGLDPAKVNPNGSGISLGHPVGATG
HHHHHHHHCCCCEECCHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCC
AIITTKAIAELHRTGGRYALVTMCIGGGQGIAAIFERV
HHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
TSAHRDILIASIARTAIGTFGGSLKDIPNTQLATTAVRAAIERSGIAADAVGHVVMGNV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
IPTDTKDAYLSRVAAIDAGCPVETPAFNVNRLCGSGLQAIVSAAQAIALGDCDVAIGGGS
CCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEECCCC
ESMSRGPYFDTASRWGARMGDAKSIDYMLGILHDPWQKMHMGITAENVAERYRIGREAQD
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHHH
QLAAESQRRAAAAIAAGYFKEQIVPVEIPGRKGTATLFDTDEHVRGGTTVEVLAGMKPAF
HHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCCCCCCCEEHHHCCCHHH
RKDGGTVTAGNASGINDGAAAVVLVAGDRAAALGARPLARLVGYAHAGVEPAYMGIGPVP
HCCCCEEEECCCCCCCCCCEEEEEEECCCHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCH
ATRAVLQRTGLSVQDMDVIEANEAFAAQACAVIQELGLDPAKVNPNGSGISLGHPVGATG
HHHHHHHHCCCCEECCHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCCCC
AIITTKAIAELHRTGGRYALVTMCIGGGQGIAAIFERV
HHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]