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The map label for this gene is polC [H]

Identifier: 120599313

GI number: 120599313

Start: 2873716

End: 2874612

Strand: Direct

Name: polC [H]

Synonym: Sputw3181_2509

Alternate gene names: 120599313

Gene position: 2873716-2874612 (Clockwise)

Preceding gene: 120599312

Following gene: 120599315

Centisome position: 61.03

GC content: 43.26

Gene sequence:

>897_bases
ATGGATAAAGAAACCGCAGAGTTTTTAGCAAAACACCCCTACATCCCTACCAAATTTATCGCATTAGATGCCGAGACCAC
GGGGCTTGAGGCTGAAACGGACAAGATAATAGAGATTGCCGCAATCAAATTTGATCTCCTATCTAACGATCACCCTGTAT
TTGAGTCATTGATCAATCCTGCGGTAAAAGTACCGCGCAAAATAACCAATATCACAGGCATCACCAACAAGATGCTGGTA
GACCAGCCGCCCTTTGCAGAGCTCGCGCAAGACTTAAAAGCATTTATCGGTGATCTACCTATCGTGGCCTACAATGCCAA
GTTTGATAAAGGCTTTTTAGATGCTGAGTTCGCTAATGTTGGCATCGTGCTCAATAACCAGTATCACTGTGCATTAAATC
TGGCAAAGGCAGCATTTAGTTTACCTAACTACAAATTAGCTACGGTAGCCAAGCACTGTAATATTCCGCTTGATGAAGCA
CATCGCGCTAAAGCTGATGCCATTGCCGCTGGCCGAGTGTTTATGTGTGCCGCAGTCACCCTTGGGCATATTAACGAAAT
CAAACCTAAATCAGCATTTGGTAAAACCAAAGGCGCTGATCATAAAGCTTACCCACCCAATGAATATGGTGAACTATACG
GCCAAACCATCGTCTTTACGGGCGAACTATCAATGACCAGAGCCGAAGCCTTTGAAGCCGCCGCAGAACTCGGGCTTGAA
ATTAAATCAGGCGTATCTAAAAAGACTGATTATCTTGTCGTTGGTGAACAGGATGAAAGTCTAGTTGGCCCAAGCGGCAT
ATCTAGTAAGCAACTAAAAGCACAAGATCTGATTGATGAAGGCTTTGATATTCAAATTTTAGATGAAGATGAATTTATGG
AGCTAATTGAAGGATAA

Upstream 100 bases:

>100_bases
ATTAGATAAGCATAAAATATACAGAATTCATGTTAACTATGATGGTGCAGGTCTGCAAATATTAGCTGGCGGTACTTATA
AAGACTAAAGGAATAGTAAA

Downstream 100 bases:

>100_bases
AACCTAAATAAAATTTATTTTTAAACCCGCCTAAGCGGGTTTATCAAAAAAAGATAATGTTATGGCTCTTGTGTACCATT
GCCTCCATTGCCACCGCCTG

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDKETAEFLAKHPYIPTKFIALDAETTGLEAETDKIIEIAAIKFDLLSNDHPVFESLINPAVKVPRKITNITGITNKMLV
DQPPFAELAQDLKAFIGDLPIVAYNAKFDKGFLDAEFANVGIVLNNQYHCALNLAKAAFSLPNYKLATVAKHCNIPLDEA
HRAKADAIAAGRVFMCAAVTLGHINEIKPKSAFGKTKGADHKAYPPNEYGELYGQTIVFTGELSMTRAEAFEAAAELGLE
IKSGVSKKTDYLVVGEQDESLVGPSGISSKQLKAQDLIDEGFDIQILDEDEFMELIEG

Sequences:

>Translated_298_residues
MDKETAEFLAKHPYIPTKFIALDAETTGLEAETDKIIEIAAIKFDLLSNDHPVFESLINPAVKVPRKITNITGITNKMLV
DQPPFAELAQDLKAFIGDLPIVAYNAKFDKGFLDAEFANVGIVLNNQYHCALNLAKAAFSLPNYKLATVAKHCNIPLDEA
HRAKADAIAAGRVFMCAAVTLGHINEIKPKSAFGKTKGADHKAYPPNEYGELYGQTIVFTGELSMTRAEAFEAAAELGLE
IKSGVSKKTDYLVVGEQDESLVGPSGISSKQLKAQDLIDEGFDIQILDEDEFMELIEG
>Mature_298_residues
MDKETAEFLAKHPYIPTKFIALDAETTGLEAETDKIIEIAAIKFDLLSNDHPVFESLINPAVKVPRKITNITGITNKMLV
DQPPFAELAQDLKAFIGDLPIVAYNAKFDKGFLDAEFANVGIVLNNQYHCALNLAKAAFSLPNYKLATVAKHCNIPLDEA
HRAKADAIAAGRVFMCAAVTLGHINEIKPKSAFGKTKGADHKAYPPNEYGELYGQTIVFTGELSMTRAEAFEAAAELGLE
IKSGVSKKTDYLVVGEQDESLVGPSGISSKQLKAQDLIDEGFDIQILDEDEFMELIEG

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2176

COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1786409, Length=110, Percent_Identity=36.3636363636364, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 32461; Mature: 32461

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50172 BRCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKETAEFLAKHPYIPTKFIALDAETTGLEAETDKIIEIAAIKFDLLSNDHPVFESLINP
CCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AVKVPRKITNITGITNKMLVDQPPFAELAQDLKAFIGDLPIVAYNAKFDKGFLDAEFANV
HHHCCHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECEE
GIVLNNQYHCALNLAKAAFSLPNYKLATVAKHCNIPLDEAHRAKADAIAAGRVFMCAAVT
EEEECCCEEEEEEHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHH
LGHINEIKPKSAFGKTKGADHKAYPPNEYGELYGQTIVFTGELSMTRAEAFEAAAELGLE
HHHHHCCCCCHHCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCH
IKSGVSKKTDYLVVGEQDESLVGPSGISSKQLKAQDLIDEGFDIQILDEDEFMELIEG
HHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHCC
>Mature Secondary Structure
MDKETAEFLAKHPYIPTKFIALDAETTGLEAETDKIIEIAAIKFDLLSNDHPVFESLINP
CCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHH
AVKVPRKITNITGITNKMLVDQPPFAELAQDLKAFIGDLPIVAYNAKFDKGFLDAEFANV
HHHCCHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECEE
GIVLNNQYHCALNLAKAAFSLPNYKLATVAKHCNIPLDEAHRAKADAIAAGRVFMCAAVT
EEEECCCEEEEEEHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHH
LGHINEIKPKSAFGKTKGADHKAYPPNEYGELYGQTIVFTGELSMTRAEAFEAAAELGLE
HHHHHCCCCCHHCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCH
IKSGVSKKTDYLVVGEQDESLVGPSGISSKQLKAQDLIDEGFDIQILDEDEFMELIEG
HHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]