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The map label for this gene is rnt [H]

Identifier: 120599226

GI number: 120599226

Start: 2794881

End: 2795552

Strand: Direct

Name: rnt [H]

Synonym: Sputw3181_2422

Alternate gene names: 120599226

Gene position: 2794881-2795552 (Clockwise)

Preceding gene: 120599216

Following gene: 120599228

Centisome position: 59.36

GC content: 44.94

Gene sequence:

>672_bases
ATGAGCGACATTTGCGACGCGAACAAATTGAAACACCGATTTCGAGGCTACTTTCCGGTAGTCATTGATGTCGAAACGGC
GGGCTTCAACTCTCAAACGGATGCGTTGCTGGAAATAGCCGTCACCCTGCTGAAAATGGATGATGAGGGCATGCTAGGCA
TTGATAAAACCTTGCACTTCCATATAGAACCATTTGAAGGCGCCAATTTAGAACCTGCAGCACTCGCTTTTAATGGGATA
GATCCTAACAATCCATTACGTGGCGCTGTCAGCGAAAAAGAGGCATTTTTAGACATTTTTAAAGCTGTCAAAAAGGCACA
AAAAGCTGCTGATTGTCATCGCTGTATTATTGTGGCCCATAATGCCGCTTTTGATCATGGTTTTGTCAGTAAAGCGATTG
AGCGTTGTGACTTAAAACGCTCACCATTTCATCCTTTTGCCACCTTCGATACCGCTACACTCGCAGGACTTGCTATTGGT
CATACGGTTTTAGCGAAAGCATGTATGATGGCAGGTATTCCCTTTGACAATAAAGAAGCCCACTCAGCGTTATATGATAC
TGAACGCACAGCGGAGCTCTTTTGCTACATAGTCAATCGCTGGAAACAATTAGGCGGTTGGCCTTTACTGGCTGCAGGTG
CGCAAGATGCTGAAAGTGACACAGAAGAATAA

Upstream 100 bases:

>100_bases
AACTATTTAGTTCCTTTAGCTTATAAAACCACCAATCAAGCATGACACACCCAAGTGCATTTAGCATAATAGCGCCCTGT
GAATTTTATTGGGTATCTTA

Downstream 100 bases:

>100_bases
ACTCAATCACTGAAGCTAGATTTATTCAATAAAAACAAAAAGCTGCCAATTGGCAGCTTTTTTATTCACTCGCTCTATTC
GATTACAGAGAGCTTGCGTT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSDICDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGMLGIDKTLHFHIEPFEGANLEPAALAFNGI
DPNNPLRGAVSEKEAFLDIFKAVKKAQKAADCHRCIIVAHNAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIG
HTVLAKACMMAGIPFDNKEAHSALYDTERTAELFCYIVNRWKQLGGWPLLAAGAQDAESDTEE

Sequences:

>Translated_223_residues
MSDICDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGMLGIDKTLHFHIEPFEGANLEPAALAFNGI
DPNNPLRGAVSEKEAFLDIFKAVKKAQKAADCHRCIIVAHNAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIG
HTVLAKACMMAGIPFDNKEAHSALYDTERTAELFCYIVNRWKQLGGWPLLAAGAQDAESDTEE
>Mature_222_residues
SDICDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGMLGIDKTLHFHIEPFEGANLEPAALAFNGID
PNNPLRGAVSEKEAFLDIFKAVKKAQKAADCHRCIIVAHNAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIGH
TVLAKACMMAGIPFDNKEAHSALYDTERTAELFCYIVNRWKQLGGWPLLAAGAQDAESDTEE

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=212, Percent_Identity=69.811320754717, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24468; Mature: 24337

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDICDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGMLGIDKTLHF
CCCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEE
HIEPFEGANLEPAALAFNGIDPNNPLRGAVSEKEAFLDIFKAVKKAQKAADCHRCIIVAH
EECCCCCCCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
NAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIGHTVLAKACMMAGIPFDNKEA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTERTAELFCYIVNRWKQLGGWPLLAAGAQDAESDTEE
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SDICDANKLKHRFRGYFPVVIDVETAGFNSQTDALLEIAVTLLKMDDEGMLGIDKTLHF
CCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEE
HIEPFEGANLEPAALAFNGIDPNNPLRGAVSEKEAFLDIFKAVKKAQKAADCHRCIIVAH
EECCCCCCCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
NAAFDHGFVSKAIERCDLKRSPFHPFATFDTATLAGLAIGHTVLAKACMMAGIPFDNKEA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HSALYDTERTAELFCYIVNRWKQLGGWPLLAAGAQDAESDTEE
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA