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The map label for this gene is melA [H]

Identifier: 120599159

GI number: 120599159

Start: 2716213

End: 2717253

Strand: Direct

Name: melA [H]

Synonym: Sputw3181_2355

Alternate gene names: 120599159

Gene position: 2716213-2717253 (Clockwise)

Preceding gene: 120599158

Following gene: 120599160

Centisome position: 57.69

GC content: 43.23

Gene sequence:

>1041_bases
ATGGCAAGCGAACTCAATCCACTGGGCTTATTAGGTATCGAATTCACGGAATTCGCCAGCCCTGATACCGATTTTATGCA
CAAAGTCTTTATCGACTTTGGTTTTTCACTGCTGAAAAAAACCAAGCACAAAGACATCCTGTACTACAAACAGAATGATA
TTAATTTTCTGCTCAACACTGAGCGCGAAGGTTTCTCAGCTAAGTTTGCTAAATCCCACGGCCCAGCCATTTGTTCTATG
GGCTGGCGTGTAGAAGATGCCGGCTTTGCCTATCGTGTTGCCGTTGAACGTGGTGCAAAACCCGCCGATGATGCCAATAA
AGATCTGCCTTATCCTGCGATTTACGGCATTGGCGACAGCTTAATTTATTTCATCGACACCTTCGGTGCAGATAACAATA
TCTATGCCACTGATTTTGAAGATTTAGATGAGCAAATTATCACTCAAGAGAAAGGCTTTATCGAGGTCGATCACTTAACC
AACAACGTCTACAAAGGCACAATGGAACATTGGGCTAACTTCTATAAAAACATTTTTGGTTTTACTGAGGTGCGTTACTT
TGACATCAGCGGCGTACAAACCGCGCTAGTGTCCTACGCCCTGCGCTCACCCGATGGCAGCTTCTGTATCCCGATTAACG
AAGGTAAAGGCAACGATAAAAACCAAATCGATGAATATCTGAAGGAATACAATGGCCCAGGTGTGCAGCATTTAGCCTTT
AGAAGCCGCGATATCGTTAAATCCTTAGATGCGATGGAAGGCAGCTCAATCCAATGCCTAGACATCATTCCTGAATACTA
CGACACTATTTTCGATAAAGTGCCACAAGTCACTGAGAATCGTGAGCGCATTAAACATCACCAAATTCTGGTGGATGGTG
ATGATACAGGCTATTTACTGCAGATATTTACTAAAAACCTGTTTGGACCGATTTTCATTGAAATCATCCAACGTAAGAAT
AACTTAGGTTTTGGTGAAGGCAACTTTACTGCCCTGTTTGAATCAATTGAACGGGACCAAATGCGCCGCGGCGTTCTATA
A

Upstream 100 bases:

>100_bases
AACCGCTCTTGGCTAGAAGACTAGCCCCAAGCCACCAGCACTCCCATGCGGGAGTCATTAGAGTGACGGCGTGTTGCACT
AAAATAACGAGGAAAACATC

Downstream 100 bases:

>100_bases
ATATCATCCCATCCATAAGAACGGTACGCACTGCGTACCGTTTCTGTTTTTACGCATAGCAAAAATCCATTTGATGAAGC
GGTTTAAACAAGCCCATTGG

Product: 4-hydroxyphenylpyruvate dioxygenase

Products: homogentisate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MASELNPLGLLGIEFTEFASPDTDFMHKVFIDFGFSLLKKTKHKDILYYKQNDINFLLNTEREGFSAKFAKSHGPAICSM
GWRVEDAGFAYRVAVERGAKPADDANKDLPYPAIYGIGDSLIYFIDTFGADNNIYATDFEDLDEQIITQEKGFIEVDHLT
NNVYKGTMEHWANFYKNIFGFTEVRYFDISGVQTALVSYALRSPDGSFCIPINEGKGNDKNQIDEYLKEYNGPGVQHLAF
RSRDIVKSLDAMEGSSIQCLDIIPEYYDTIFDKVPQVTENRERIKHHQILVDGDDTGYLLQIFTKNLFGPIFIEIIQRKN
NLGFGEGNFTALFESIERDQMRRGVL

Sequences:

>Translated_346_residues
MASELNPLGLLGIEFTEFASPDTDFMHKVFIDFGFSLLKKTKHKDILYYKQNDINFLLNTEREGFSAKFAKSHGPAICSM
GWRVEDAGFAYRVAVERGAKPADDANKDLPYPAIYGIGDSLIYFIDTFGADNNIYATDFEDLDEQIITQEKGFIEVDHLT
NNVYKGTMEHWANFYKNIFGFTEVRYFDISGVQTALVSYALRSPDGSFCIPINEGKGNDKNQIDEYLKEYNGPGVQHLAF
RSRDIVKSLDAMEGSSIQCLDIIPEYYDTIFDKVPQVTENRERIKHHQILVDGDDTGYLLQIFTKNLFGPIFIEIIQRKN
NLGFGEGNFTALFESIERDQMRRGVL
>Mature_345_residues
ASELNPLGLLGIEFTEFASPDTDFMHKVFIDFGFSLLKKTKHKDILYYKQNDINFLLNTEREGFSAKFAKSHGPAICSMG
WRVEDAGFAYRVAVERGAKPADDANKDLPYPAIYGIGDSLIYFIDTFGADNNIYATDFEDLDEQIITQEKGFIEVDHLTN
NVYKGTMEHWANFYKNIFGFTEVRYFDISGVQTALVSYALRSPDGSFCIPINEGKGNDKNQIDEYLKEYNGPGVQHLAFR
SRDIVKSLDAMEGSSIQCLDIIPEYYDTIFDKVPQVTENRERIKHHQILVDGDDTGYLLQIFTKNLFGPIFIEIIQRKNN
LGFGEGNFTALFESIERDQMRRGVL

Specific function: Unknown

COG id: COG3185

COG function: function code ER; 4-hydroxyphenylpyruvate dioxygenase and related hemolysins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4HPPD family [H]

Homologues:

Organism=Homo sapiens, GI4504477, Length=320, Percent_Identity=29.375, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI285002264, Length=320, Percent_Identity=29.375, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI14249394, Length=212, Percent_Identity=25.9433962264151, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555220, Length=203, Percent_Identity=37.9310344827586, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17550752, Length=206, Percent_Identity=36.4077669902913, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI21356105, Length=299, Percent_Identity=32.7759197324415, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24667510, Length=299, Percent_Identity=32.7759197324415, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005956
- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 1.13.11.27

Molecular weight: Translated: 39436; Mature: 39305

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASELNPLGLLGIEFTEFASPDTDFMHKVFIDFGFSLLKKTKHKDILYYKQNDINFLLNT
CCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEC
EREGFSAKFAKSHGPAICSMGWRVEDAGFAYRVAVERGAKPADDANKDLPYPAIYGIGDS
CCCCCCHHHHHCCCCEEEECCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCEEECCCCC
LIYFIDTFGADNNIYATDFEDLDEQIITQEKGFIEVDHLTNNVYKGTMEHWANFYKNIFG
EEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEECHHCHHHHHHHHHHHHHHHHHHC
FTEVRYFDISGVQTALVSYALRSPDGSFCIPINEGKGNDKNQIDEYLKEYNGPGVQHLAF
HHEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHH
RSRDIVKSLDAMEGSSIQCLDIIPEYYDTIFDKVPQVTENRERIKHHQILVDGDDTGYLL
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEECCCCCHHH
QIFTKNLFGPIFIEIIQRKNNLGFGEGNFTALFESIERDQMRRGVL
HHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASELNPLGLLGIEFTEFASPDTDFMHKVFIDFGFSLLKKTKHKDILYYKQNDINFLLNT
CCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEC
EREGFSAKFAKSHGPAICSMGWRVEDAGFAYRVAVERGAKPADDANKDLPYPAIYGIGDS
CCCCCCHHHHHCCCCEEEECCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCEEECCCCC
LIYFIDTFGADNNIYATDFEDLDEQIITQEKGFIEVDHLTNNVYKGTMEHWANFYKNIFG
EEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEECHHCHHHHHHHHHHHHHHHHHHC
FTEVRYFDISGVQTALVSYALRSPDGSFCIPINEGKGNDKNQIDEYLKEYNGPGVQHLAF
HHEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHH
RSRDIVKSLDAMEGSSIQCLDIIPEYYDTIFDKVPQVTENRERIKHHQILVDGDDTGYLL
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEECCCCCHHH
QIFTKNLFGPIFIEIIQRKNNLGFGEGNFTALFESIERDQMRRGVL
HHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylpyruvate; O2

Specific reaction: 4-hydroxyphenylpyruvate + O2 = homogentisate + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1756973 [H]