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The map label for this gene is yhaR [H]

Identifier: 120599112

GI number: 120599112

Start: 2661739

End: 2662605

Strand: Direct

Name: yhaR [H]

Synonym: Sputw3181_2308

Alternate gene names: 120599112

Gene position: 2661739-2662605 (Clockwise)

Preceding gene: 120599111

Following gene: 120599113

Centisome position: 56.53

GC content: 51.56

Gene sequence:

>867_bases
ATGACTGACACACAACACCAGAACACTAGCACTCAATCCTTAGGCAATTTGCAGCACGCCTTAGACCATTTGCAACATGT
AAGTTACGCGCTGAATAACGGCGTAGGCGAGTTAATCCTTAACCGAGCCGAAGTGCACAACGCCTTCGATGAGGTGATGA
TTAGCGAAATGATCGTCGTCCTCGGTTATTTTGCCGAGCACAAAGACTGCAAACTGTTGGTATTAAAAGCCAATGGCAAA
AACTTCAGCGCTGGCGCCGACTTAAATTGGATGCGCAAACAAGCAAAGATGGATTTCGATCAAAACCTCAATGACGCCAA
AGCACTGGCTAAGCTGATGCAGGATTTAGATACCTTTCCAAAACCCACTATCGCCTTAATCCAAGGTGCGGCCTTTGGCG
GCGCGCTTGGGTTAATCTGCGCCAGTGATATTGCCATCGCCACCGAGCGTGCCAGTTTCTGCTTAAGCGAAGTCAAACTA
GGGCTGATCCCTGCGGTGATTAGCCCCTACGTTGCCCGCGCTATGGGCAATCGCGCCTCACGCCGCTATATGCTGACCGC
AGAGCGTTTTGATGCGCAAACAGCGCTCAAACTCAATGTTATCCATGAGATAAACGACGATTTAGCGGCCGCAGCCCAAC
CCATTATCACCGCGCTGCTGGCCAACAGTCCGCAGGGAATGGCATGGGTAAAGACCCTACTCGCCCGCCTCGAAGACGGC
GTGATAGACCAAGATACGATTGATTACACCAGCGAGCGCATCGCCCGCATTCGGGTATCTGATGAAGGCCAAGAGGGCTT
AAACGCCTTTTTTGAGAAACGTCAGCCGAACTGGCACACGCCAACCGATACCCAAGGAGCACTTTAA

Upstream 100 bases:

>100_bases
CCTATCGAAGACACCCGCTTCGGCGTCTTCCGTATGTAACGCATCTTGCGATGCCTTACGTATCACTCATAGAGGGAATA
ACGATGACGGGAATAAAATA

Downstream 100 bases:

>100_bases
TGTTGACCAAGCAGATGTTGACTAACAGCATGTTCACTAAGCTTTTAATTGCTAACCGTGGTGAAATTGCCTGCCGTATT
ATCAAAACCGCGCAGGCCAT

Product: enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTDTQHQNTSTQSLGNLQHALDHLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIVVLGYFAEHKDCKLLVLKANGK
NFSAGADLNWMRKQAKMDFDQNLNDAKALAKLMQDLDTFPKPTIALIQGAAFGGALGLICASDIAIATERASFCLSEVKL
GLIPAVISPYVARAMGNRASRRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALLANSPQGMAWVKTLLARLEDG
VIDQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGAL

Sequences:

>Translated_288_residues
MTDTQHQNTSTQSLGNLQHALDHLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIVVLGYFAEHKDCKLLVLKANGK
NFSAGADLNWMRKQAKMDFDQNLNDAKALAKLMQDLDTFPKPTIALIQGAAFGGALGLICASDIAIATERASFCLSEVKL
GLIPAVISPYVARAMGNRASRRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALLANSPQGMAWVKTLLARLEDG
VIDQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGAL
>Mature_287_residues
TDTQHQNTSTQSLGNLQHALDHLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIVVLGYFAEHKDCKLLVLKANGKN
FSAGADLNWMRKQAKMDFDQNLNDAKALAKLMQDLDTFPKPTIALIQGAAFGGALGLICASDIAIATERASFCLSEVKLG
LIPAVISPYVARAMGNRASRRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALLANSPQGMAWVKTLLARLEDGV
IDQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGAL

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=270, Percent_Identity=23.7037037037037, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=30.46875, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=27.4193548387097, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=27.7551020408163, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=25.9842519685039, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=26.7924528301887, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=26.615969581749, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=30.0751879699248, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790281, Length=175, Percent_Identity=26.8571428571429, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=214, Percent_Identity=30.8411214953271, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=278, Percent_Identity=25.5395683453237, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=22.1343873517787, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=25, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=28, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=173, Percent_Identity=31.2138728323699, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24653477, Length=173, Percent_Identity=31.2138728323699, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=22.7091633466135, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 31604; Mature: 31473

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQHQNTSTQSLGNLQHALDHLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LGYFAEHKDCKLLVLKANGKNFSAGADLNWMRKQAKMDFDQNLNDAKALAKLMQDLDTFP
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
KPTIALIQGAAFGGALGLICASDIAIATERASFCLSEVKLGLIPAVISPYVARAMGNRAS
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALLANSPQGMAWVKTLLARLEDG
CEEEEEHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
VIDQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGAL
CCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTQHQNTSTQSLGNLQHALDHLQHVSYALNNGVGELILNRAEVHNAFDEVMISEMIVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LGYFAEHKDCKLLVLKANGKNFSAGADLNWMRKQAKMDFDQNLNDAKALAKLMQDLDTFP
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
KPTIALIQGAAFGGALGLICASDIAIATERASFCLSEVKLGLIPAVISPYVARAMGNRAS
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RRYMLTAERFDAQTALKLNVIHEINDDLAAAAQPIITALLANSPQGMAWVKTLLARLEDG
CEEEEEHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
VIDQDTIDYTSERIARIRVSDEGQEGLNAFFEKRQPNWHTPTDTQGAL
CCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061 [H]