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The map label for this gene is ppx [H]

Identifier: 120599097

GI number: 120599097

Start: 2643757

End: 2645310

Strand: Direct

Name: ppx [H]

Synonym: Sputw3181_2293

Alternate gene names: 120599097

Gene position: 2643757-2645310 (Clockwise)

Preceding gene: 120599096

Following gene: 120599099

Centisome position: 56.15

GC content: 44.34

Gene sequence:

>1554_bases
TTGGTTGCCACACATTCACAACCGCGACACTTTGTCGCCATCGACATGGGATCAAATAGTTTTCACCTTGTTATTGCCCG
TGAGCAAGATGGCAGTTTGCAGATATTACACAAAGAAAAACAACAAGTTCAGCTAGCTACTGGGTTAGATACACAAAATA
TGTTAAGCCAAGAGGCGATGGAACGAGGCCTTAATTGCCTGCGTGATTTTAATCAACGCTTTTCAAACCTTGACCAAGCA
CAAGTGCGTTTAGTGGCAACTCATACCCTTAGGGTGGCTAAAAATCGTGAGCAGTTTATTGAAGCAGCGCTTGCCATTTT
GCCCTATCCAGTGGAAGTCATTTCTGGTCATGAGGAAGCGCGCTTAATTTATAACGGTATTGCCCAAAGCCAAGTATTGG
GAAAACGTAATCTCGTTATCGATATTGGTGGTGGCTCTACCGAGGTCGTGCTTGGACAGAAAAATACTCCCACCCATTTA
TCCAGCTTGCGCTGTGGCTGTGTCAGTTTCAACGAACGCTTCTTCCCAAATGGACATATTACACCAGCCTCCTTTCGCGC
CGCACAAAGTGCTGCAGATAAGCAGTTTGCTTCCTTATCAAAAGAATACTTCAGCGGTCATTGGGACTTAGTCCTGGGCA
GTTCGGGAACCGTCAAAGCCATATGTGAAGCCGTTCAAGAAGATCACGGTGATGAAGTCATTACCTTAACGCGGCTGAAA
CAACTCAAGTTGAAATTAATCAGATTTGGGCACATTGATCATGTCCAATTCACCCATGTGGATAACAAACGCACACCGTT
AATCTCCGCAGGTCTTGCTATTTTAATTAGTTTTTTTAGACGCTTACCTGTTGAAAAGTTGGAGTTTAGCCCTGGGGCGC
TGCGAGAAGGCGTGTTATATGAGTTAGCTAAAATTGGCCAATATCAAGATATACGCCACCGCACTGTGGATAGCATTGCC
CAGCTTTACCATATCGATATGCCCCACGCGATTAAGGTACGAGATACTGCGATGGCATTGTTTGAACAGGTTGCCGATGA
ATGGCAATTAAGGCCCCATGCTCGATTACTCGCCTATGCCGCCACCTTACATGAGATAGGTTTACACATAAATTCAAGGG
CATTGCATAAACACGGCGCCTACATCATTAGTAATAGCGATATGCCAGGGTTTAGTGAGGCATTACAGCAAGATCTAGCG
CGACTGATTGTCAACCATCGTAAAAAACCCTATGAGTTAATGCTTGCTGAATTAGAGCCAAATCATAAATTGACGCTCAT
TCGCTTACTCTGTCTATTACGCCTTGCTGTTCTGCTCAATCTTGGCCGTATCGCCAAATCATTGATTTTGGAACAAATTC
AAGTTATTCCAGATGGCGTTATGTTAGTGCTACCCAGCCATAAACGAAAAATAAGCCTTTTTATGAAAGACTTGCAGCGA
GAGCAAAAGCAAATGGCCGCTCTTGGAATAAATCTGCGTTTAGTGTCGAGTATCGCCTCCCACAACGATAAACACTCACT
TGAACCAGAAATCACCACTGACACTGTGGGATAA

Upstream 100 bases:

>100_bases
TCCTCCGGGGAGTTTACCCTAGAGAAAAAACCAGAATTACAGGCCAAAGCCAGTTAATCACTGGCTTTATTATCATCTCG
ATTGCCTAAGGATAATCCGA

Downstream 100 bases:

>100_bases
TCATCGCCATTCTCCCAAGAGAAAAAGGCTCGTATTACAAAACTAGCGCCGCATCATACGAGCCTTTGGATGACATTTGT
TTAGTATTGCAACGGCTTAT

Product: Ppx/GppA phosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MVATHSQPRHFVAIDMGSNSFHLVIAREQDGSLQILHKEKQQVQLATGLDTQNMLSQEAMERGLNCLRDFNQRFSNLDQA
QVRLVATHTLRVAKNREQFIEAALAILPYPVEVISGHEEARLIYNGIAQSQVLGKRNLVIDIGGGSTEVVLGQKNTPTHL
SSLRCGCVSFNERFFPNGHITPASFRAAQSAADKQFASLSKEYFSGHWDLVLGSSGTVKAICEAVQEDHGDEVITLTRLK
QLKLKLIRFGHIDHVQFTHVDNKRTPLISAGLAILISFFRRLPVEKLEFSPGALREGVLYELAKIGQYQDIRHRTVDSIA
QLYHIDMPHAIKVRDTAMALFEQVADEWQLRPHARLLAYAATLHEIGLHINSRALHKHGAYIISNSDMPGFSEALQQDLA
RLIVNHRKKPYELMLAELEPNHKLTLIRLLCLLRLAVLLNLGRIAKSLILEQIQVIPDGVMLVLPSHKRKISLFMKDLQR
EQKQMAALGINLRLVSSIASHNDKHSLEPEITTDTVG

Sequences:

>Translated_517_residues
MVATHSQPRHFVAIDMGSNSFHLVIAREQDGSLQILHKEKQQVQLATGLDTQNMLSQEAMERGLNCLRDFNQRFSNLDQA
QVRLVATHTLRVAKNREQFIEAALAILPYPVEVISGHEEARLIYNGIAQSQVLGKRNLVIDIGGGSTEVVLGQKNTPTHL
SSLRCGCVSFNERFFPNGHITPASFRAAQSAADKQFASLSKEYFSGHWDLVLGSSGTVKAICEAVQEDHGDEVITLTRLK
QLKLKLIRFGHIDHVQFTHVDNKRTPLISAGLAILISFFRRLPVEKLEFSPGALREGVLYELAKIGQYQDIRHRTVDSIA
QLYHIDMPHAIKVRDTAMALFEQVADEWQLRPHARLLAYAATLHEIGLHINSRALHKHGAYIISNSDMPGFSEALQQDLA
RLIVNHRKKPYELMLAELEPNHKLTLIRLLCLLRLAVLLNLGRIAKSLILEQIQVIPDGVMLVLPSHKRKISLFMKDLQR
EQKQMAALGINLRLVSSIASHNDKHSLEPEITTDTVG
>Mature_517_residues
MVATHSQPRHFVAIDMGSNSFHLVIAREQDGSLQILHKEKQQVQLATGLDTQNMLSQEAMERGLNCLRDFNQRFSNLDQA
QVRLVATHTLRVAKNREQFIEAALAILPYPVEVISGHEEARLIYNGIAQSQVLGKRNLVIDIGGGSTEVVLGQKNTPTHL
SSLRCGCVSFNERFFPNGHITPASFRAAQSAADKQFASLSKEYFSGHWDLVLGSSGTVKAICEAVQEDHGDEVITLTRLK
QLKLKLIRFGHIDHVQFTHVDNKRTPLISAGLAILISFFRRLPVEKLEFSPGALREGVLYELAKIGQYQDIRHRTVDSIA
QLYHIDMPHAIKVRDTAMALFEQVADEWQLRPHARLLAYAATLHEIGLHINSRALHKHGAYIISNSDMPGFSEALQQDLA
RLIVNHRKKPYELMLAELEPNHKLTLIRLLCLLRLAVLLNLGRIAKSLILEQIQVIPDGVMLVLPSHKRKISLFMKDLQR
EQKQMAALGINLRLVSSIASHNDKHSLEPEITTDTVG

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=483, Percent_Identity=41.8219461697723, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI48994966, Length=431, Percent_Identity=41.0672853828306, Blast_Score=317, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 58012; Mature: 58012

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVATHSQPRHFVAIDMGSNSFHLVIAREQDGSLQILHKEKQQVQLATGLDTQNMLSQEAM
CCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECHHHHEEHHCCCCHHHHHHHHHH
ERGLNCLRDFNQRFSNLDQAQVRLVATHTLRVAKNREQFIEAALAILPYPVEVISGHEEA
HHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHH
RLIYNGIAQSQVLGKRNLVIDIGGGSTEVVLGQKNTPTHLSSLRCGCVSFNERFFPNGHI
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHCCEEECCCCCCCCCCC
TPASFRAAQSAADKQFASLSKEYFSGHWDLVLGSSGTVKAICEAVQEDHGDEVITLTRLK
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHH
QLKLKLIRFGHIDHVQFTHVDNKRTPLISAGLAILISFFRRLPVEKLEFSPGALREGVLY
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHH
ELAKIGQYQDIRHRTVDSIAQLYHIDMPHAIKVRDTAMALFEQVADEWQLRPHARLLAYA
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHH
ATLHEIGLHINSRALHKHGAYIISNSDMPGFSEALQQDLARLIVNHRKKPYELMLAELEP
HHHHHHCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEECCC
NHKLTLIRLLCLLRLAVLLNLGRIAKSLILEQIQVIPDGVMLVLPSHKRKISLFMKDLQR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
EQKQMAALGINLRLVSSIASHNDKHSLEPEITTDTVG
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVATHSQPRHFVAIDMGSNSFHLVIAREQDGSLQILHKEKQQVQLATGLDTQNMLSQEAM
CCCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEECHHHHEEHHCCCCHHHHHHHHHH
ERGLNCLRDFNQRFSNLDQAQVRLVATHTLRVAKNREQFIEAALAILPYPVEVISGHEEA
HHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHH
RLIYNGIAQSQVLGKRNLVIDIGGGSTEVVLGQKNTPTHLSSLRCGCVSFNERFFPNGHI
HHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHCCEEECCCCCCCCCCC
TPASFRAAQSAADKQFASLSKEYFSGHWDLVLGSSGTVKAICEAVQEDHGDEVITLTRLK
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHH
QLKLKLIRFGHIDHVQFTHVDNKRTPLISAGLAILISFFRRLPVEKLEFSPGALREGVLY
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHH
ELAKIGQYQDIRHRTVDSIAQLYHIDMPHAIKVRDTAMALFEQVADEWQLRPHARLLAYA
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHH
ATLHEIGLHINSRALHKHGAYIISNSDMPGFSEALQQDLARLIVNHRKKPYELMLAELEP
HHHHHHCCCCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEECCC
NHKLTLIRLLCLLRLAVLLNLGRIAKSLILEQIQVIPDGVMLVLPSHKRKISLFMKDLQR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
EQKQMAALGINLRLVSSIASHNDKHSLEPEITTDTVG
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]