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The map label for this gene is ywbI [H]

Identifier: 120599099

GI number: 120599099

Start: 2646833

End: 2647714

Strand: Direct

Name: ywbI [H]

Synonym: Sputw3181_2295

Alternate gene names: 120599099

Gene position: 2646833-2647714 (Clockwise)

Preceding gene: 120599097

Following gene: 120599103

Centisome position: 56.22

GC content: 44.22

Gene sequence:

>882_bases
ATGGATATACGTCAACTCAAACATTTAGACGCCCTTGCTAACACTGGCAGTTTTACTGCCGCGGCTAAAAAACTCAATAT
GGCGCAACCTGCGCTCAGTCAGAGTATTAAACGTCTTGAGCAATCCCTAGGTGTTACCTTAATCAATCGTACTAGCCATA
AAAATGACAAACTACTGGCCCTCACCGCTGAAGGTTTAGTACTCCATCAACATGCCACGCTTATCCTAAAGCAAATCAAA
CAGGCCGAGGCACAAATCCATGCAATGGCCAATCTCACCTCGGGTGAAGTGCGTATTGCAGTGCCGGGTATGCTCGGTTC
CTTCTATTTACCCTCGCGGTTAATGGCCTTTCGCCATCAATACCCCGAGCTAAAACTGTCACTGTTTGAAGGCGGCACCC
GCGATGCACTACGTATGTTACAGCAAGAAGAAGTGGATATTGCGATTATTACCGCCCAAGATCTACAGAGTGATTTTGAC
AGTCAACTGTTAATCCGTGAACAAATGGTGATTGCAATGGGGACAGAACATCCATTAGCAGAACATAATGCTGTTAGCTT
GAGCGATTTTTTTGACCATGAATTAGTGATGTTTAAGACAGGTTATTTTCACCGTGAATGGATACTGTCACAGGCAAAAG
TGTTAGGGAAAAAGCCCAATATTGCCTTTGAAACCAATCTGATTAACTTGATAAAACAAATTGTATCCCAAGGCTTTGGT
ATCACCAGTGTGCTGGAAATGGCGATCAGCCCGAAGGACGATATTTTAGCTAAACCCTTCGATCCACCCGTCTTTTTAAA
TCTGCATATTGCGTGGAAAAAACAGCGTCCAGTCAGTCAAGCCGACCGTGCTTTTGTCGAGTTTCTGATGAAGAATCGCT
AA

Upstream 100 bases:

>100_bases
TTAGCACTGGGCATAACATAACAATAATAGATAATTAGTATCGTAATTATTGATTTTTGTTATAGATAGATCTCATTAAT
AACTCTGTGTGAGCCAATAG

Downstream 100 bases:

>100_bases
TAAAAAAGCCCTTTCAAATCTAAAAGGGCTTAATCACTTATCAGTTTTATCGGTTAGCCATAACGGCTTTCATCAACACT
TCAGTAGTGTATTTATCATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDIRQLKHLDALANTGSFTAAAKKLNMAQPALSQSIKRLEQSLGVTLINRTSHKNDKLLALTAEGLVLHQHATLILKQIK
QAEAQIHAMANLTSGEVRIAVPGMLGSFYLPSRLMAFRHQYPELKLSLFEGGTRDALRMLQQEEVDIAIITAQDLQSDFD
SQLLIREQMVIAMGTEHPLAEHNAVSLSDFFDHELVMFKTGYFHREWILSQAKVLGKKPNIAFETNLINLIKQIVSQGFG
ITSVLEMAISPKDDILAKPFDPPVFLNLHIAWKKQRPVSQADRAFVEFLMKNR

Sequences:

>Translated_293_residues
MDIRQLKHLDALANTGSFTAAAKKLNMAQPALSQSIKRLEQSLGVTLINRTSHKNDKLLALTAEGLVLHQHATLILKQIK
QAEAQIHAMANLTSGEVRIAVPGMLGSFYLPSRLMAFRHQYPELKLSLFEGGTRDALRMLQQEEVDIAIITAQDLQSDFD
SQLLIREQMVIAMGTEHPLAEHNAVSLSDFFDHELVMFKTGYFHREWILSQAKVLGKKPNIAFETNLINLIKQIVSQGFG
ITSVLEMAISPKDDILAKPFDPPVFLNLHIAWKKQRPVSQADRAFVEFLMKNR
>Mature_293_residues
MDIRQLKHLDALANTGSFTAAAKKLNMAQPALSQSIKRLEQSLGVTLINRTSHKNDKLLALTAEGLVLHQHATLILKQIK
QAEAQIHAMANLTSGEVRIAVPGMLGSFYLPSRLMAFRHQYPELKLSLFEGGTRDALRMLQQEEVDIAIITAQDLQSDFD
SQLLIREQMVIAMGTEHPLAEHNAVSLSDFFDHELVMFKTGYFHREWILSQAKVLGKKPNIAFETNLINLIKQIVSQGFG
ITSVLEMAISPKDDILAKPFDPPVFLNLHIAWKKQRPVSQADRAFVEFLMKNR

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=263, Percent_Identity=31.9391634980989, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790399, Length=266, Percent_Identity=27.4436090225564, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=27.7966101694915, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787601, Length=199, Percent_Identity=30.1507537688442, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=26.7123287671233, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=29.1338582677165, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=27.1255060728745, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788481, Length=297, Percent_Identity=24.9158249158249, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788887, Length=257, Percent_Identity=27.6264591439689, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=27.4590163934426, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=23.046875, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI2367136, Length=276, Percent_Identity=25, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788296, Length=249, Percent_Identity=27.710843373494, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=236, Percent_Identity=27.9661016949153, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=22.4913494809689, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789639, Length=132, Percent_Identity=28.7878787878788, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789506, Length=159, Percent_Identity=27.6729559748428, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32931; Mature: 32931

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRQLKHLDALANTGSFTAAAKKLNMAQPALSQSIKRLEQSLGVTLINRTSHKNDKLLA
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEE
LTAEGLVLHQHATLILKQIKQAEAQIHAMANLTSGEVRIAVPGMLGSFYLPSRLMAFRHQ
EEECCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
YPELKLSLFEGGTRDALRMLQQEEVDIAIITAQDLQSDFDSQLLIREQMVIAMGTEHPLA
CCCEEEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHHHHHEEECCCCCCC
EHNAVSLSDFFDHELVMFKTGYFHREWILSQAKVLGKKPNIAFETNLINLIKQIVSQGFG
CCCCEEHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCC
ITSVLEMAISPKDDILAKPFDPPVFLNLHIAWKKQRPVSQADRAFVEFLMKNR
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIRQLKHLDALANTGSFTAAAKKLNMAQPALSQSIKRLEQSLGVTLINRTSHKNDKLLA
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEE
LTAEGLVLHQHATLILKQIKQAEAQIHAMANLTSGEVRIAVPGMLGSFYLPSRLMAFRHQ
EEECCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
YPELKLSLFEGGTRDALRMLQQEEVDIAIITAQDLQSDFDSQLLIREQMVIAMGTEHPLA
CCCEEEEEECCCCHHHHHHHHHCCCCEEEEEHHHHHCCCCHHHHHHHHHHEEECCCCCCC
EHNAVSLSDFFDHELVMFKTGYFHREWILSQAKVLGKKPNIAFETNLINLIKQIVSQGFG
CCCCEEHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCC
ITSVLEMAISPKDDILAKPFDPPVFLNLHIAWKKQRPVSQADRAFVEFLMKNR
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]