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The map label for this gene is rimK [H]

Identifier: 120599085

GI number: 120599085

Start: 2626403

End: 2627398

Strand: Direct

Name: rimK [H]

Synonym: Sputw3181_2281

Alternate gene names: 120599085

Gene position: 2626403-2627398 (Clockwise)

Preceding gene: 120599084

Following gene: 120599086

Centisome position: 55.78

GC content: 44.48

Gene sequence:

>996_bases
ATGCGCGGTTGGATTTTATATAAAGAAACCGCGACTCAGCTAAAACCTGAATGGTATGAAATTGAGCGTCTGCTGGCTGC
GGCTAAGGCTGACAATATAGAGCTAACAGTTTACGCCCCTGATGAATTTGATCTTACGGTCACTAGAGAAGATAACAAGA
GTATTTTATTGAATGGGCAAGCCGTTGAGCTGCCTGATTTTATCATTCCAAGAATGGGTTCAGGGACGACCTATTTTGCC
CTGGCCATTATTCGCCATTTAGAGCGTTTAGGGGTGTATTGCCTTAACCCGTCTAAAGCGATCGAAATTGTAAAAGACAA
ACTATTCTCCCAGCAGTTGCTGGCCGAGAAAAACTTGCCAACACCCAAAACCATGTTGGTTAAGTTTCCGGTCGATATCG
ATCTCGTGGAACGCCATTTGGGCTTTCCAGTGGTGATAAAAACGCTGTCAGGATCGCAGGGCAGCGGCGTGTTTTTATCC
CATAAGGCACGCGAATTTGACGATTTGATGCAATTGATTGAAGCGACAAATAAAAACGCTAACATCATTTTGCAGGAGTT
TATTGCCAATAGCCATGGCCGCGATTTACGGGTCTTTACTATCGGTGGCAGAGTGGCAGGTTGTTATGAAAGGCGTGGTC
AGGAAGATAGCTTTAAAGCCAATGTTAGTGCTGGTGGCTCGGCGCGCCCCTTTCAGATAACCCCTGAAATTGAATGGCTA
GCCACACAAACAGCAAATATTCTCGATCTTGATGTAGCAGGCATCGATCTCTTATTTGATAACGGCCATTATAAAATTTG
TGAGGCAAACTCATCTCCGGGATTTGAAGGGTTAGAATCTTGTTTGAATGTCGATATTGCCGCGCAGATTTTACACTTTA
TTCGCATCCGTCTAGGAATGTTCAATAAAGATGACTTGAGCAGTACTGATGGCTCGATGACTCAGACTGAGCCAACTTCA
CCCGAGGCAAAACTCGTATCACTCAAGACGAAATAA

Upstream 100 bases:

>100_bases
AAGTCGAGCCAGAAGTGATATTTGATGTTGATATTGAAGATGACGTTCAGGATGAGCCTGAACCCGAGGATAAAGCATGA
AAATTAAGGTTTGCGACTAA

Downstream 100 bases:

>100_bases
CCTCCATTAAGTTTTGCTCTAATAATCTGCGATGATTAGCAATGTGATCATCGCAGATTGCTATCAATCCAGTTAACCTC
CCTGATTTCTATCCTTTGGC

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIELTVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIDLVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDDLSSTDGSMTQTEPTS
PEAKLVSLKTK

Sequences:

>Translated_331_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIELTVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIDLVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDDLSSTDGSMTQTEPTS
PEAKLVSLKTK
>Mature_331_residues
MRGWILYKETATQLKPEWYEIERLLAAAKADNIELTVYAPDEFDLTVTREDNKSILLNGQAVELPDFIIPRMGSGTTYFA
LAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLPTPKTMLVKFPVDIDLVERHLGFPVVIKTLSGSQGSGVFLS
HKAREFDDLMQLIEATNKNANIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGMFNKDDLSSTDGSMTQTEPTS
PEAKLVSLKTK

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI134254462, Length=298, Percent_Identity=30.5369127516779, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI164663810, Length=298, Percent_Identity=28.5234899328859, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1787076, Length=242, Percent_Identity=36.7768595041322, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGWILYKETATQLKPEWYEIERLLAAAKADNIELTVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECC
AVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
EEECCHHHCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIDLVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEEECCCHHHHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
CEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDDLSSTDGSMTQTEPTSPEAKLVSLKTK
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRGWILYKETATQLKPEWYEIERLLAAAKADNIELTVYAPDEFDLTVTREDNKSILLNGQ
CCCEEEEECHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEECC
AVELPDFIIPRMGSGTTYFALAIIRHLERLGVYCLNPSKAIEIVKDKLFSQQLLAEKNLP
EEECCHHHCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCC
TPKTMLVKFPVDIDLVERHLGFPVVIKTLSGSQGSGVFLSHKAREFDDLMQLIEATNKNA
CCCEEEEEECCCHHHHHHHCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCC
NIILQEFIANSHGRDLRVFTIGGRVAGCYERRGQEDSFKANVSAGGSARPFQITPEIEWL
CEEHHHHHCCCCCCEEEEEEECCCEEHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEE
ATQTANILDLDVAGIDLLFDNGHYKICEANSSPGFEGLESCLNVDIAAQILHFIRIRLGM
EECCCCEEEEEECCEEEEEECCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
FNKDDLSSTDGSMTQTEPTSPEAKLVSLKTK
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA