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The map label for this gene is rnhA

Identifier: 120599050

GI number: 120599050

Start: 2593844

End: 2594314

Strand: Direct

Name: rnhA

Synonym: Sputw3181_2246

Alternate gene names: 120599050

Gene position: 2593844-2594314 (Clockwise)

Preceding gene: 120599047

Following gene: 120599053

Centisome position: 55.09

GC content: 46.28

Gene sequence:

>471_bases
ATGACTGAACGAAAACTGATCCACATCTTCACTGACGGCTCTTGCTTAGGCAACCCCGGCCCCGGTGGCTATGGCATTGT
AATGAATTATAAGGGTCATACAAAGGAGATGTCCGACGGTTTTGCACTTACCACCAATAATCGCATGGAATTGTTGGCAC
CAATCATCGCCCTAGAAAGTCTTAAAGAACCTTGCAGAGTCGTTCTTACCAGTGATAGTCAATATATGCGCCAAGGCATA
ATGACGTGGATCCACGGTTGGAAGAAAAAAGGGTGGATGACCTCAAACCGTACGCCTGTCAAAAATGTGGATCTTTGGAA
ACGCTTAGATAAAGTATCGCAAATGCACACTATCGATTGGCAATGGGTAAAAGGCCACGCTGGCCATGCTGAAAATGAGC
GTTGTGATATCCTAGCACGCTCCGCGGCTGAAGCGAATCCAACCCAAATCGATGAAGGTTATCAACCTTAA

Upstream 100 bases:

>100_bases
CTGGCGGCTGGCGTTTGAAATAATATTCATACTTTTATGCGCTTCTTATGACAATTTTTGGTTATACTCGGCCGAAATTC
ACGGCAATGGTAACAACTTG

Downstream 100 bases:

>100_bases
GCACACTCCTCTAAATCACTAGAGATAACGAGATAATAAATATTCTGTTTTACGCACTCACTTATCGTAAACATTGTGTG
GTATCAATCTCTAAAAGCCT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTERKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALESLKEPCRVVLTSDSQYMRQGI
MTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKVSQMHTIDWQWVKGHAGHAENERCDILARSAAEANPTQIDEGYQP

Sequences:

>Translated_156_residues
MTERKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALESLKEPCRVVLTSDSQYMRQGI
MTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKVSQMHTIDWQWVKGHAGHAENERCDILARSAAEANPTQIDEGYQP
>Mature_155_residues
TERKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALESLKEPCRVVLTSDSQYMRQGIM
TWIHGWKKKGWMTSNRTPVKNVDLWKRLDKVSQMHTIDWQWVKGHAGHAENERCDILARSAAEANPTQIDEGYQP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=144, Percent_Identity=34.0277777777778, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=65.5629139072848, Blast_Score=218, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=36.1702127659575, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=34.7222222222222, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=34.7222222222222, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17685; Mature: 17554

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALES
CCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKEPCRVVLTSDSQYMRQGIMTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKVSQMHTIDW
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
QWVKGHAGHAENERCDILARSAAEANPTQIDEGYQP
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TERKLIHIFTDGSCLGNPGPGGYGIVMNYKGHTKEMSDGFALTTNNRMELLAPIIALES
CCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LKEPCRVVLTSDSQYMRQGIMTWIHGWKKKGWMTSNRTPVKNVDLWKRLDKVSQMHTIDW
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
QWVKGHAGHAENERCDILARSAAEANPTQIDEGYQP
EEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA