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The map label for this gene is adhE [H]

Identifier: 120599019

GI number: 120599019

Start: 2558687

End: 2561290

Strand: Direct

Name: adhE [H]

Synonym: Sputw3181_2214

Alternate gene names: 120599019

Gene position: 2558687-2561290 (Clockwise)

Preceding gene: 120599015

Following gene: 120599024

Centisome position: 54.34

GC content: 47.93

Gene sequence:

>2604_bases
ATGTTAGTGACCAATGCACAAGAACTGGATCTGATGGTCGAGCGTGTCGCCAAGGCACAGGCCGAGTATGCAACCTTCAG
TCAAGAACAAGTGGATGCGATATTTCGCGCAGCCGCCCTCGCCGCTGCGGATGCGCGTATCTCGCTAGCCAAAATGGCAG
CGGCAGAAACACGTATGGGTGTTATTGAGGACAAGGTGATCAAAAACCACTTTGCCTCTGAATATATTTACAACAAGTAT
AAAGATGAGAAAACCTGCGGTATTTTGTCTGAAGATCTCACCTTTGGCACAATCACTATCGCCGAGCCAGTTGGCATTAT
TTGCGGCATAGTGCCCACGACTAACCCAACATCTACGGCAATATTCAAGGCCTTGATTAGCCTTAAAACCCGCAATGCCA
TTATTTTTTCGCCCCATCCAAGGGCTAAAGCCTCAACCACCACTGCCGCAAAATTAGTGCTTGATGCTGCCGTGGCCGCT
GGCGCACCGAAAGATATTATTGGCTGGATTGATGAGCCTAGCGTCGCACTATCAAATCAGCTAATGACTCACCCTAAGGT
TAATTTGATCCTTGCCACGGGCGGCCCTGGTATGGTGAAAGCCGCCTACTCCTCAGGTAAACCTGCCATTGGTGTGGGCG
CAGGAAATACCCCTATCGTTATCGATGAAACCGCCGATATAAAACGCGCGGTGAGTTCTATATTGATGTCCAAAACCTTC
GATAACGGGGTGGTTTGCGCATCTGAGCAAGCCGTGATCGTTGTCGATAGCATTTATGAGCAAGTGAAAGAACGCTTCGC
AAGCCATGGGGGTTATATCTTATCGGCAAGTGAAACCGCGGCAATGCAAAACGTCATCCTCAAAAATGGTGGCTTAAATG
CCGATATCGTGGGGCAAAGTGCGGCGAGTATTGCCAAAATGGCGGGGGTTGAAGTACCTCATTGGACTAAGGTGCTGATT
GGTGAAGTGACTGATATTAGTGAAAGCGAAGCCTTCGCCCATGAAAAATTATCGCCACTGCTTGGTATGTACCGCGCTAA
AGACTTTAACGATGCCGTCGATCAAGCCGAAGCCTTAGTTGCACTTGGGGGGATTGGCCATACATCAGGACTTTACACCG
ACCAAGACACTCAAACAGAACGCGTTAAAACCTTTGGTTTTAGAATGAAAACCGCACGAATTTTAATCAACACTCCCGCT
TCACAAGGTGGTATTGGCGATCTCTATAACTTTAAATTGGCCCCGTCTCTCACACTCGGTTGCGGTTCTTGGGGCGGCAA
CTCGATTTCAGAAAACGTGGGTCCAAGTCATTTGATCAACAAAAAAACCGTCGCTAAGAGGGCCGAAAACATGCTGTGGC
ACAAGCTTCCTTCATCTATTTATTTCCGCCGTGGCAGTCTTCCTATCGCCCTTGAGGAATTAAGTGATAAAAAACGCGCG
CTGATCGTCACAGATAGATTTTTGTTCAACCAAGGTTATTGCAACGAAACTATTCGCATTTTAAAGGCTCAAGGCTTAGA
AACTGAAATTTTCTATGATGTCGAAGCCGATCCCACCTTAGCCATTGTGCGCCAAGGGGCAAAGGTTGCCTGTAGTTTTA
AACCCGATGTCATCATCGCCCTCGGCGGTGGCTCACCCATGGATGCCGCCAAGATTATTTGGGTGATGTACGAACATCCT
GAGGTTGATTTTGCCGATCTCGCATTGCGCTTTATGGATATCCGTAAACGTATCTACAAATTCCCAAAAATGGGCACAAA
AGCCATGATGGTGGCTATTCCGACAACCTCAGGCACAGGCTCAGAAGTCACACCTTTTGCGGTAGTTACCGATGAGCAAA
CAGGTAAAAAATACCCCATTGCTGATTATCAACTCACGCCCAATATGGCGATTGTTGACCCCAATCTTGTGATGGATATG
CCTAAGTCACTCACCGCCTTTGGTGGAATTGATGCGGTGACCCATGCACTTGAAGCCTATGTTAGCGTAATGGCGAATGA
GTACAGCGATGGCCAAGCACTGCAAGCCCTCGATTTATTGTGCAAATATTTGCCGGATGCCTATAACTTAGGTGCACAAT
CCCCAGTCGCCCGTGAAAAGGTTCATAATGGTGCCACCATTGCGGGTATCGCCTTTGCAAACGCATTTCTTGGGATTTGC
CACTCGATGGCGCACAAACTCGGTGCCGAGTTTCATCTCGCCCACGGCTTAGCCAATGCGCTGCTGATCAACAATGTGAT
ACGTTTTAACGCAACGGATCTGCCCACAAAGCAAGCGGCGTTTAGCCAATATGACCGACCCAAAGCCTTGTGCCGTTATG
CAGCCATCGCCGACCATTTGAAACTAGGCGGAAAATCGGATGAAGAGAAAGTGGAAAAACTGCTTGAGAAAATCACACAG
CTCAAGAAGACGATTGGCATTCCTGCTTCTATCCAAGAAGCCGGTGTCAACGAAGCCGACTTCCTCGCCAAGTTAGATGT
ACTCGCCGAAGATGCCTTTGACGATCAATGCACAGGTGCCAACCCACGTTATCCATTGATTAACGAGTTAAAACAGTTAT
TACTCGATAGTTACTATGGTCGCGATTATAGCGATAACAACTAG

Upstream 100 bases:

>100_bases
GTATGCTCACACTTTATGAGTGAGTTTAAGTGTAATTTTCTACCATAAAGAAGATTTAGTGAACATGAGCATTCCCTAAA
TCTTTTTAGGAGTGTTAATC

Downstream 100 bases:

>100_bases
GCTATCACCTAGATAAAAGCAGCACTATATCAACGAAGAAGCCCTCCAATGAGGGCTTTTTAATGCATGTCTTACCATAA
GGATGAATACTTGCGTAACG

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 867; Mature: 867

Protein sequence:

>867_residues
MLVTNAQELDLMVERVAKAQAEYATFSQEQVDAIFRAAALAAADARISLAKMAAAETRMGVIEDKVIKNHFASEYIYNKY
KDEKTCGILSEDLTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNAIIFSPHPRAKASTTTAAKLVLDAAVAA
GAPKDIIGWIDEPSVALSNQLMTHPKVNLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
DNGVVCASEQAVIVVDSIYEQVKERFASHGGYILSASETAAMQNVILKNGGLNADIVGQSAASIAKMAGVEVPHWTKVLI
GEVTDISESEAFAHEKLSPLLGMYRAKDFNDAVDQAEALVALGGIGHTSGLYTDQDTQTERVKTFGFRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
LIVTDRFLFNQGYCNETIRILKAQGLETEIFYDVEADPTLAIVRQGAKVACSFKPDVIIALGGGSPMDAAKIIWVMYEHP
EVDFADLALRFMDIRKRIYKFPKMGTKAMMVAIPTTSGTGSEVTPFAVVTDEQTGKKYPIADYQLTPNMAIVDPNLVMDM
PKSLTAFGGIDAVTHALEAYVSVMANEYSDGQALQALDLLCKYLPDAYNLGAQSPVAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLAHGLANALLINNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIADHLKLGGKSDEEKVEKLLEKITQ
LKKTIGIPASIQEAGVNEADFLAKLDVLAEDAFDDQCTGANPRYPLINELKQLLLDSYYGRDYSDNN

Sequences:

>Translated_867_residues
MLVTNAQELDLMVERVAKAQAEYATFSQEQVDAIFRAAALAAADARISLAKMAAAETRMGVIEDKVIKNHFASEYIYNKY
KDEKTCGILSEDLTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNAIIFSPHPRAKASTTTAAKLVLDAAVAA
GAPKDIIGWIDEPSVALSNQLMTHPKVNLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
DNGVVCASEQAVIVVDSIYEQVKERFASHGGYILSASETAAMQNVILKNGGLNADIVGQSAASIAKMAGVEVPHWTKVLI
GEVTDISESEAFAHEKLSPLLGMYRAKDFNDAVDQAEALVALGGIGHTSGLYTDQDTQTERVKTFGFRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
LIVTDRFLFNQGYCNETIRILKAQGLETEIFYDVEADPTLAIVRQGAKVACSFKPDVIIALGGGSPMDAAKIIWVMYEHP
EVDFADLALRFMDIRKRIYKFPKMGTKAMMVAIPTTSGTGSEVTPFAVVTDEQTGKKYPIADYQLTPNMAIVDPNLVMDM
PKSLTAFGGIDAVTHALEAYVSVMANEYSDGQALQALDLLCKYLPDAYNLGAQSPVAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLAHGLANALLINNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIADHLKLGGKSDEEKVEKLLEKITQ
LKKTIGIPASIQEAGVNEADFLAKLDVLAEDAFDDQCTGANPRYPLINELKQLLLDSYYGRDYSDNN
>Mature_867_residues
MLVTNAQELDLMVERVAKAQAEYATFSQEQVDAIFRAAALAAADARISLAKMAAAETRMGVIEDKVIKNHFASEYIYNKY
KDEKTCGILSEDLTFGTITIAEPVGIICGIVPTTNPTSTAIFKALISLKTRNAIIFSPHPRAKASTTTAAKLVLDAAVAA
GAPKDIIGWIDEPSVALSNQLMTHPKVNLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
DNGVVCASEQAVIVVDSIYEQVKERFASHGGYILSASETAAMQNVILKNGGLNADIVGQSAASIAKMAGVEVPHWTKVLI
GEVTDISESEAFAHEKLSPLLGMYRAKDFNDAVDQAEALVALGGIGHTSGLYTDQDTQTERVKTFGFRMKTARILINTPA
SQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
LIVTDRFLFNQGYCNETIRILKAQGLETEIFYDVEADPTLAIVRQGAKVACSFKPDVIIALGGGSPMDAAKIIWVMYEHP
EVDFADLALRFMDIRKRIYKFPKMGTKAMMVAIPTTSGTGSEVTPFAVVTDEQTGKKYPIADYQLTPNMAIVDPNLVMDM
PKSLTAFGGIDAVTHALEAYVSVMANEYSDGQALQALDLLCKYLPDAYNLGAQSPVAREKVHNGATIAGIAFANAFLGIC
HSMAHKLGAEFHLAHGLANALLINNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIADHLKLGGKSDEEKVEKLLEKITQ
LKKTIGIPASIQEAGVNEADFLAKLDVLAEDAFDDQCTGANPRYPLINELKQLLLDSYYGRDYSDNN

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=313, Percent_Identity=26.517571884984, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1787493, Length=867, Percent_Identity=77.8546712802768, Blast_Score=1373, Evalue=0.0,
Organism=Escherichia coli, GI1789163, Length=400, Percent_Identity=32.5, Blast_Score=203, Evalue=5e-53,
Organism=Escherichia coli, GI48994951, Length=391, Percent_Identity=36.5728900255754, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1788797, Length=336, Percent_Identity=33.9285714285714, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI87082107, Length=382, Percent_Identity=31.151832460733, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1789386, Length=388, Percent_Identity=24.7422680412371, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=280, Percent_Identity=28.2142857142857, Blast_Score=107, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321181, Length=391, Percent_Identity=34.5268542199489, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24657991, Length=420, Percent_Identity=24.047619047619, Blast_Score=80, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 93418; Mature: 93418

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTNAQELDLMVERVAKAQAEYATFSQEQVDAIFRAAALAAADARISLAKMAAAETRMG
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIEDKVIKNHFASEYIYNKYKDEKTCGILSEDLTFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCHHCEEECCCCEEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNAIIFSPHPRAKASTTTAAKLVLDAAVAAGAPKDIIGWIDEPSVALSNQ
HHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMTHPKVNLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
HHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVVCASEQAVIVVDSIYEQVKERFASHGGYILSASETAAMQNVILKNGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCEECCCH
AASIAKMAGVEVPHWTKVLIGEVTDISESEAFAHEKLSPLLGMYRAKDFNDAVDQAEALV
HHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALGGIGHTSGLYTDQDTQTERVKTFGFRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
HCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCCE
LIVTDRFLFNQGYCNETIRILKAQGLETEIFYDVEADPTLAIVRQGAKVACSFKPDVIIA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHEECCCCEEEEECCCCEEEE
LGGGSPMDAAKIIWVMYEHPEVDFADLALRFMDIRKRIYKFPKMGTKAMMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGKKYPIADYQLTPNMAIVDPNLVMDMPKSLTAFGGIDAVTHALEAY
CCCCCEEEEECCCCCCCCCCCCEEECCCEEEECCHHHHHCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLCKYLPDAYNLGAQSPVAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLAHGLANALLINNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIADHL
HHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
KLGGKSDEEKVEKLLEKITQLKKTIGIPASIQEAGVNEADFLAKLDVLAEDAFDDQCTGA
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
NPRYPLINELKQLLLDSYYGRDYSDNN
CCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLVTNAQELDLMVERVAKAQAEYATFSQEQVDAIFRAAALAAADARISLAKMAAAETRMG
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIEDKVIKNHFASEYIYNKYKDEKTCGILSEDLTFGTITIAEPVGIICGIVPTTNPTSTA
HHHHHHHHHHHHHHHHHHHCCCCCHHCEEECCCCEEEEEECCCCCEEEECCCCCCCHHHH
IFKALISLKTRNAIIFSPHPRAKASTTTAAKLVLDAAVAAGAPKDIIGWIDEPSVALSNQ
HHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMTHPKVNLILATGGPGMVKAAYSSGKPAIGVGAGNTPIVIDETADIKRAVSSILMSKTF
HHCCCCEEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVVCASEQAVIVVDSIYEQVKERFASHGGYILSASETAAMQNVILKNGGLNADIVGQS
CCCEEEECCCCEEEHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCEECCCH
AASIAKMAGVEVPHWTKVLIGEVTDISESEAFAHEKLSPLLGMYRAKDFNDAVDQAEALV
HHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALGGIGHTSGLYTDQDTQTERVKTFGFRMKTARILINTPASQGGIGDLYNFKLAPSLTLG
HCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNSISENVGPSHLINKKTVAKRAENMLWHKLPSSIYFRRGSLPIALEELSDKKRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEHHHCCCCCCE
LIVTDRFLFNQGYCNETIRILKAQGLETEIFYDVEADPTLAIVRQGAKVACSFKPDVIIA
EEEEEHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHEECCCCEEEEECCCCEEEE
LGGGSPMDAAKIIWVMYEHPEVDFADLALRFMDIRKRIYKFPKMGTKAMMVAIPTTSGTG
ECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
SEVTPFAVVTDEQTGKKYPIADYQLTPNMAIVDPNLVMDMPKSLTAFGGIDAVTHALEAY
CCCCCEEEEECCCCCCCCCCCCEEECCCEEEECCHHHHHCCHHHHHHCCHHHHHHHHHHH
VSVMANEYSDGQALQALDLLCKYLPDAYNLGAQSPVAREKVHNGATIAGIAFANAFLGIC
HHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHHHHHH
HSMAHKLGAEFHLAHGLANALLINNVIRFNATDLPTKQAAFSQYDRPKALCRYAAIADHL
HHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
KLGGKSDEEKVEKLLEKITQLKKTIGIPASIQEAGVNEADFLAKLDVLAEDAFDDQCTGA
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
NPRYPLINELKQLLLDSYYGRDYSDNN
CCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]