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The map label for this gene is estB [H]

Identifier: 120599000

GI number: 120599000

Start: 2534487

End: 2535680

Strand: Direct

Name: estB [H]

Synonym: Sputw3181_2193

Alternate gene names: 120599000

Gene position: 2534487-2535680 (Clockwise)

Preceding gene: 120598992

Following gene: 120599001

Centisome position: 53.83

GC content: 56.28

Gene sequence:

>1194_bases
ATGACAACCAACACATTTCCCCTGCCTCGAAGCGTAGTGCCCATACCGCTCTCAGCCCGCATCCAAAACGTTATTGAGCA
AGCATTACATGAGCAACGATTAGTGGGTGCTGTCGTGCTGGTCGCTCGGAACTGTGAGCTTATCTACCAGCAGGCAGCAG
GTTATGCCGACCGGGAAAGTGCAAGGCCGATGGCGGTGGATACGGTTTTTCGCCTGGCCTCGGTGAGTAAACCTATTGTC
TCTGCCGCCGCACTGGTATTGGTAGCACAAGGTCGACTAGAGCTGGACGAGAACATTGGCAACTGGCTGCCTGATTTTCA
GCCAAGGTTGGCCGATGGCCGCCTAGCCCGGATCACCGTTCGGCAGTTGCTTAGCCATACGGCCGGCTTGGGTTATCGCT
TTTTCGAAGCCGACGAAAAGAGCCCCTACGCGTTGGTGGGTGTATCGGATGGCATGGATGCGTCGGGCATTAGCCTGCAG
GAAAACCTTAAGCGCATCGCCAGTGTGTCGCTGTTGTATGAGCCGGGCACAGCTTGGGGCTATTCGCTGGCAACCGATGT
GCTGGGTGCATTGATTGAGCGCGTTTATGGTACCTCGCTAAACGAAGCAATACACCATTTGGTGACCGGCCCCTTAGGCA
TGAATGATACCGGTTTTGTCGCAGGTGATCCGCAGCGCATGGCCACCGCTTATGTTAACGATGCACCACAGCCACATCAA
CTGGCAGAAGGGGAAACCGTCTCACCTTTTGAGGGCACAATTGGTATCAAATACAGCCCAGCTCGTATCTTCGATGCACA
GTCTTTTCCATCCGCAGGTGCAGGCATGGCGGGCACGGCCGGGGATTTTCTGCGCCTGTTGGAAGCCCTGCGTCAAGAAG
GCGGCGGGCTATTGCCCAAAGAACTGGTGGCAGAAATGGCGCGGGATCAGACAGGAGGATTGGAACTGCCTAATTCACCG
GGTTTTGGTTTTGGCCTGGGGTTCTCGGTGCTGCGTGACCCAAAACTTGCCGCTTCTCCCGAGTCAGCAGGCACATGGCG
CTGGGGCGGTGCTTACGGCCATTCCTGGTTTGTGGATCGGGCCCAGGGGCTTAGTATGGTGGCTTTTACCAATACCCTGT
ATGAGGGCATGTGTGGCCGTTTCGTTACTGGGCTACGGGATGCCGTGTATGACGGGTCTGAGGTGCAGCGATGA

Upstream 100 bases:

>100_bases
CGGACACAAAGCTCCGTATATAACGGAACTATACCCTACTTTTTCTTTAATCTAAGGCTTTATATGATCATGGCATAACC
AATGAATAAAGGACTTTGCT

Downstream 100 bases:

>100_bases
AGTCCACTGCACCAGATCGTCTGCCACTGGCCGCTCTGCTGGCGCTGGCCTTAGCTGGTTTTGTTACCATCCTGACCGAG
GCGTTACCCGCCGGCCTTTT

Product: beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTTNTFPLPRSVVPIPLSARIQNVIEQALHEQRLVGAVVLVARNCELIYQQAAGYADRESARPMAVDTVFRLASVSKPIV
SAAALVLVAQGRLELDENIGNWLPDFQPRLADGRLARITVRQLLSHTAGLGYRFFEADEKSPYALVGVSDGMDASGISLQ
ENLKRIASVSLLYEPGTAWGYSLATDVLGALIERVYGTSLNEAIHHLVTGPLGMNDTGFVAGDPQRMATAYVNDAPQPHQ
LAEGETVSPFEGTIGIKYSPARIFDAQSFPSAGAGMAGTAGDFLRLLEALRQEGGGLLPKELVAEMARDQTGGLELPNSP
GFGFGLGFSVLRDPKLAASPESAGTWRWGGAYGHSWFVDRAQGLSMVAFTNTLYEGMCGRFVTGLRDAVYDGSEVQR

Sequences:

>Translated_397_residues
MTTNTFPLPRSVVPIPLSARIQNVIEQALHEQRLVGAVVLVARNCELIYQQAAGYADRESARPMAVDTVFRLASVSKPIV
SAAALVLVAQGRLELDENIGNWLPDFQPRLADGRLARITVRQLLSHTAGLGYRFFEADEKSPYALVGVSDGMDASGISLQ
ENLKRIASVSLLYEPGTAWGYSLATDVLGALIERVYGTSLNEAIHHLVTGPLGMNDTGFVAGDPQRMATAYVNDAPQPHQ
LAEGETVSPFEGTIGIKYSPARIFDAQSFPSAGAGMAGTAGDFLRLLEALRQEGGGLLPKELVAEMARDQTGGLELPNSP
GFGFGLGFSVLRDPKLAASPESAGTWRWGGAYGHSWFVDRAQGLSMVAFTNTLYEGMCGRFVTGLRDAVYDGSEVQR
>Mature_396_residues
TTNTFPLPRSVVPIPLSARIQNVIEQALHEQRLVGAVVLVARNCELIYQQAAGYADRESARPMAVDTVFRLASVSKPIVS
AAALVLVAQGRLELDENIGNWLPDFQPRLADGRLARITVRQLLSHTAGLGYRFFEADEKSPYALVGVSDGMDASGISLQE
NLKRIASVSLLYEPGTAWGYSLATDVLGALIERVYGTSLNEAIHHLVTGPLGMNDTGFVAGDPQRMATAYVNDAPQPHQL
AEGETVSPFEGTIGIKYSPARIFDAQSFPSAGAGMAGTAGDFLRLLEALRQEGGGLLPKELVAEMARDQTGGLELPNSPG
FGFGLGFSVLRDPKLAASPESAGTWRWGGAYGHSWFVDRAQGLSMVAFTNTLYEGMCGRFVTGLRDAVYDGSEVQR

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=377, Percent_Identity=24.4031830238727, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533026, Length=207, Percent_Identity=27.536231884058, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17550934, Length=199, Percent_Identity=28.643216080402, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 42575; Mature: 42444

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNTFPLPRSVVPIPLSARIQNVIEQALHEQRLVGAVVLVARNCELIYQQAAGYADRES
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCC
ARPMAVDTVFRLASVSKPIVSAAALVLVAQGRLELDENIGNWLPDFQPRLADGRLARITV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
RQLLSHTAGLGYRFFEADEKSPYALVGVSDGMDASGISLQENLKRIASVSLLYEPGTAWG
HHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEECCCCCCH
YSLATDVLGALIERVYGTSLNEAIHHLVTGPLGMNDTGFVAGDPQRMATAYVNDAPQPHQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCH
LAEGETVSPFEGTIGIKYSPARIFDAQSFPSAGAGMAGTAGDFLRLLEALRQEGGGLLPK
HCCCCCCCCCCCCEEEEECCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHH
ELVAEMARDQTGGLELPNSPGFGFGLGFSVLRDPKLAASPESAGTWRWGGAYGHSWFVDR
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQGLSMVAFTNTLYEGMCGRFVTGLRDAVYDGSEVQR
HCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
TTNTFPLPRSVVPIPLSARIQNVIEQALHEQRLVGAVVLVARNCELIYQQAAGYADRES
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCC
ARPMAVDTVFRLASVSKPIVSAAALVLVAQGRLELDENIGNWLPDFQPRLADGRLARITV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
RQLLSHTAGLGYRFFEADEKSPYALVGVSDGMDASGISLQENLKRIASVSLLYEPGTAWG
HHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEECCCCCCH
YSLATDVLGALIERVYGTSLNEAIHHLVTGPLGMNDTGFVAGDPQRMATAYVNDAPQPHQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCH
LAEGETVSPFEGTIGIKYSPARIFDAQSFPSAGAGMAGTAGDFLRLLEALRQEGGGLLPK
HCCCCCCCCCCCCEEEEECCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHH
ELVAEMARDQTGGLELPNSPGFGFGLGFSVLRDPKLAASPESAGTWRWGGAYGHSWFVDR
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQGLSMVAFTNTLYEGMCGRFVTGLRDAVYDGSEVQR
HCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]