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The map label for this gene is thrS [H]

Identifier: 120598808

GI number: 120598808

Start: 2310374

End: 2312302

Strand: Direct

Name: thrS [H]

Synonym: Sputw3181_1994

Alternate gene names: 120598808

Gene position: 2310374-2312302 (Clockwise)

Preceding gene: 120598806

Following gene: 120598809

Centisome position: 49.07

GC content: 45.57

Gene sequence:

>1929_bases
ATGCCTGTAATTACACTTCCCGATGGTAGCAAACGCGAGTTTGCTCATGCTGTATCGACTCTCGATGTCGCCGCCGATAT
CGGCCCTGGTCTTGCTAAAGCTTGTATCGCTGGCCGCGTTAATGGCGAATTAAAAGATGCTTGCGATCTCATTGAAACCG
ATGCTGAGCTATCAATCATTACCGCCAAAGACGAAGAAGGTGTTGAAATTCTTCGCCATTCTTGCGCGCATTTATTAGGT
CACGCGATTAAGCAAATGTGGCCAGAAACCAAGATGGCGATTGGTCCTGTTATCGATAATGGCTTTTATTATGATATCGA
CCTTGAGCACAAGCTGACTCAAGATGACATCGAAGCCTTAGAAAAGCGCATGCTTCAACTGGCAAAAACCAACTATGACG
TTGTAAAACGTGTCGTTAGCTGGCAAGAAGCCCGTGATACATTCGCGGCGCGCGGTGAAGAGTATAAGATTGCCATTCTG
GATGAAAATATCAGCAAAGACGCGACACCTGCGCTATATCATCACGAAGAATACACAGACATGTGTCGTGGACCGCACGT
ACCTAACATGCGTTTCTGCCAACATTTCAAATTAATGAGCATTGCCGGCGCTTATTGGCGTGGCAACTCAGAAAATAAGA
TGTTGCAACGTATCTATGGTACCGCATGGGCCGACAAAAAAGCCCTGAGTACACACTTAACCCGTCTTGAAGAAGCGGCT
AAGCGTGACCACCGTAAAATCGGTAAGCAGCTCGACTTGTATCATATGCAAGAAGAAGCGCCTGGTATGGTGTTTTGGCA
TAACGACGGTTGGAGCATCTTCCTCGAATTAGAAAGATTCATTCGCCGTAAGCTAAACCAATACACTTACCAAGAGGTGA
AAGGCCCATTAATGATGGATCGCGTGTTGTGGGAGCGTTCTGGTCACTGGGATAAATACTCAGAAGCCATGTTCACGACC
AGCAGTGAAAACCGCGAATATGCTATCAAGCCGATGAACTGCCCAGGCCACGTGCAGATCTTCAACCAAGGCTTGAAATC
TTACCGCGACTTGCCACTGCGTATGGCGGAATTTGGTTGCTGCCACCGTAATGAACCTTCAGGCTCATTGCATGGATTAA
TGCGTGTACGTGGTTTTACCCAAGATGATGCCCATATTTTCTGTACTGATGATCAAGTTCAAGAAGAAGTGAGTGCCTGT
ATCCAAATGGTATACGACACATATTCTACTTTCGGTTTTGAAAACATCGTCGTAAAACTGTCGACGCGCCCAGAAAAACG
TATCGGTGACGATGCCATGTGGGACAGAGCAGAAGAAGCATTAAAACAGGCTTTACGTGCAAACAACATTGAGTTCACCC
TTTTACCAGGTGAAGGTGCGTTCTACGGCCCCAAAATCGAATTTACTTTACACGATTGTTTGGACCGTGCATGGCAATGT
GGTACTGTGCAGCTCGATTATGCTTTACCAAACCGTCTTGGTGCAACTTATGTTGCCGAAGATAACAGCCGTCAGACCCC
TGTCATGATCCATCGTGCTATTTTAGGCTCTTTAGAACGATTCTTGGGTATCTTGATAGAAGAATATGCAGGACGTTTCC
CAACGTGGTTGGCCCCAATGCAAGTTGTTGTGATGAATATCACCGACAAACAGGCTGATTATGTTCAAGAAATCGTCAAA
TTCTTCAAAGAACAAGGTATTCGAGCGTCTTTTGACTTGAGGAATGAGAAAATAGGCTTTAAAATACGCGAGCACACCTT
AAGGCGTGTCCCTTATTTGTTGGTCGTTGGCGATCAAGAAATGGAAAATAAGGAAGTAGCGGTGCGTACCCGTGATGGTA
TCGATTTAGGTAAGATGCGACTTGAAGATTTCGCCACTAAAATCCATCAACAGATTTCGCTCCGTAGTCTCAAATTGTTG
GAGGAATAG

Upstream 100 bases:

>100_bases
GGCTAAGACTCCTGTACTGTCTTAACCTAGCGAGCATTTCTCTTACATTAACTAAAACCAAGTGGCCCTACGTGTGGGTC
ACCACTGGAAAGGACATTTC

Downstream 100 bases:

>100_bases
GTCATAAAGATCAAAAAAACAGCAGGGCGTCAGCCGGCCCCTAATAGAATCAATGAAGAAATCACAGGCGTACCTGAAGT
ACGTTTAACAGGCATTGATG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MPVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSIITAKDEEGVEILRHSCAHLLG
HAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIEALEKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEEYKIAIL
DENISKDATPALYHHEEYTDMCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMDRVLWERSGHWDKYSEAMFTT
SSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGCCHRNEPSGSLHGLMRVRGFTQDDAHIFCTDDQVQEEVSAC
IQMVYDTYSTFGFENIVVKLSTRPEKRIGDDAMWDRAEEALKQALRANNIEFTLLPGEGAFYGPKIEFTLHDCLDRAWQC
GTVQLDYALPNRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPMQVVVMNITDKQADYVQEIVK
FFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQEMENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLL
EE

Sequences:

>Translated_642_residues
MPVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSIITAKDEEGVEILRHSCAHLLG
HAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIEALEKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEEYKIAIL
DENISKDATPALYHHEEYTDMCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMDRVLWERSGHWDKYSEAMFTT
SSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGCCHRNEPSGSLHGLMRVRGFTQDDAHIFCTDDQVQEEVSAC
IQMVYDTYSTFGFENIVVKLSTRPEKRIGDDAMWDRAEEALKQALRANNIEFTLLPGEGAFYGPKIEFTLHDCLDRAWQC
GTVQLDYALPNRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPMQVVVMNITDKQADYVQEIVK
FFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQEMENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLL
EE
>Mature_641_residues
PVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSIITAKDEEGVEILRHSCAHLLGH
AIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIEALEKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEEYKIAILD
ENISKDATPALYHHEEYTDMCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAAK
RDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMDRVLWERSGHWDKYSEAMFTTS
SENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGCCHRNEPSGSLHGLMRVRGFTQDDAHIFCTDDQVQEEVSACI
QMVYDTYSTFGFENIVVKLSTRPEKRIGDDAMWDRAEEALKQALRANNIEFTLLPGEGAFYGPKIEFTLHDCLDRAWQCG
TVQLDYALPNRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPMQVVVMNITDKQADYVQEIVKF
FKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQEMENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLLE
E

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=618, Percent_Identity=39.8058252427184, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI38202255, Length=643, Percent_Identity=37.9471228615863, Blast_Score=441, Evalue=1e-123,
Organism=Homo sapiens, GI20070344, Length=646, Percent_Identity=35.4489164086687, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=77.5700934579439, Blast_Score=1076, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=629, Percent_Identity=38.4737678855326, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984192, Length=631, Percent_Identity=38.351822503962, Blast_Score=455, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=628, Percent_Identity=37.5796178343949, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322654, Length=446, Percent_Identity=36.322869955157, Blast_Score=300, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24583842, Length=630, Percent_Identity=38.7301587301587, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI28574141, Length=630, Percent_Identity=38.7301587301587, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=38.7301587301587, Blast_Score=465, Evalue=1e-131,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73815; Mature: 73684

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSII
CCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEE
TAKDEEGVEILRHSCAHLLGHAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIEAL
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEECCEECCCEEEEEECCCCCCHHHHHHH
EKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEEYKIAILDENISKDATPALYHHEEYTD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCHHHHH
MCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMD
HHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
RVLWERSGHWDKYSEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGC
HHHHHCCCCCCHHHHHHEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTDDQVQEEVSACIQMVYDTYSTFGFENIVVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPEKRIGDDAMWDRAEEALKQALRANNIEFTLLPGEGAFYGPKIEFTLHDCLDRAWQC
CCCCCHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEHHHHHHHHHCC
GTVQLDYALPNRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPM
CCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
QVVVMNITDKQADYVQEIVKFFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCC
MENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLLEE
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSII
CEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEE
TAKDEEGVEILRHSCAHLLGHAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIEAL
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEECCEECCCEEEEEECCCCCCHHHHHHH
EKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEEYKIAILDENISKDATPALYHHEEYTD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCHHHHH
MCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMD
HHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
RVLWERSGHWDKYSEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGC
HHHHHCCCCCCHHHHHHEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTDDQVQEEVSACIQMVYDTYSTFGFENIVVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPEKRIGDDAMWDRAEEALKQALRANNIEFTLLPGEGAFYGPKIEFTLHDCLDRAWQC
CCCCCHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEHHHHHHHHHCC
GTVQLDYALPNRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPM
CCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
QVVVMNITDKQADYVQEIVKFFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCC
MENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLLEE
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA